Abstract:
:Calcineurin phosphatase and cyclophilin A are cellular components involved in fungal morphogenesis and virulence. Their roles were investigated in the phytopathogenic fungus Botrytis cinerea using gene inactivation, drug inhibition and cDNA macroarrays approaches. First, the BCP1 gene coding for cyclophilin A was identified and inactivated by homologous recombination. The bcp1Delta null mutant obtained was still able to develop infection structures but was altered in symptom development on bean and tomato leaves. Opposite to this, calcineurin inhibition using cyclosporin A (CsA) modified hyphal morphology and prevented infection structure formation. CsA drug pattern signature on macroarrays allowed the identification of 18 calcineurin-dependent (CND) genes among 2839 B. cinerea genes. Among the co-regulated CND genes, three were shown to be organized as a physical cluster that could be involved in secondary metabolism. The signature of BCP1 inactivation on macroarrays allowed the identification of only three BCP1 cyclophilin-dependent (CPD) genes that were different from CND genes. Finally, no CsA drug pattern signature was observed in the bcp1Delta null mutant which provided a molecular target validation of the drug.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Viaud M,Brunet-Simon A,Brygoo Y,Pradier JM,Levis Cdoi
10.1046/j.1365-2958.2003.03798.xsubject
Has Abstractpub_date
2003-12-01 00:00:00pages
1451-65issue
5eissn
0950-382Xissn
1365-2958pii
3798journal_volume
50pub_type
杂志文章abstract::Helicobacter pylori is a human gastric pathogen that colonizes ∼ 50% of the world's population. It can cause gastritis, gastric or duodenal ulcers and also gastric cancer. The numerous side effects of available treatments and the emergence of antibiotic resistant strains are severe concerns that justify further resear...
journal_title:Molecular microbiology
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journal_title:Molecular microbiology
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pub_type: 评论,杂志文章
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pub_type: 杂志文章
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journal_title:Molecular microbiology
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journal_title:Molecular microbiology
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:2017-08-01 00:00:00
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journal_title:Molecular microbiology
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pub_type: 杂志文章
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更新日期:1992-08-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2020-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-05-01 00:00:00