Abstract:
:The DNA damage and stress response pathways interact to regulate cellular responses to genotoxins and environmental stresses. How these pathways interact in Schizosaccharomyces pombe is not well understood. We demonstrate that osmotic stress suppresses the DNA damage sensitivity of checkpoint mutants, and that this occurs through three distinct cell cycle delays. A delay in G2/M is dependent on Srk1. Progression through mitosis is halted by the Mad2-dependent spindle checkpoint. Finally, cytokinesis is impaired by modulating Cdc25 expression. These three delays, imposed by osmotic stress, together compensate for the loss of checkpoint signalling.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Alao JP,Huis In 't Veld PJ,Buhse F,Sunnerhagen Pdoi
10.1111/j.1365-2958.2010.07193.xsubject
Has Abstractpub_date
2010-07-01 00:00:00pages
143-57issue
1eissn
0950-382Xissn
1365-2958pii
MMI7193journal_volume
77pub_type
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