Abstract：
:Our understanding of how obligate intracellular pathogens co-opt eukaryotic cellular functions has been limited by their intractability to genetic manipulation and by the abundance of pathogen-specific genes with no known functional homologues. In this report we describe a gene expression system to characterize proteins of unknown function from the obligate intracellular bacterial pathogen Chlamydia trachomatis. We have devised a homologous recombination-based cloning strategy to construct an ordered array of Saccharomyces cerevisiae strains expressing all Chlamydia-specific genes. These strains were screened to identify chlamydial proteins that impaired various yeast cellular functions or that displayed tropism towards eukaryotic organelles. In addition, to identify bacterial factors that are secreted into the host cell, recombinant chlamydial proteins were screened for reactivity towards antisera raised against vacuolar membranes purified from infected mammalian cells. We report the identification of 34 C. trachomatis proteins that impact yeast cellular functions or are tropic for a range of eukaryotic organelles including mitochondria, nucleus and cytoplasmic lipid droplets, and a new family of Chlamydia-specific proteins that are exported from the parasitopherous vacuole. The versatility of molecular manipulations and protein expression in yeast allows for the rapid construction of comprehensive protein expression arrays to explore the function of pathogen-specific gene products from microorganisms that are difficult to genetically manipulate, grow in culture or too dangerous for routine analysis in the laboratory.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Sisko JL,Spaeth K,Kumar Y,Valdivia RHdoi
10.1111/j.1365-2958.2006.05074.xsubject
Has Abstractpub_date
2006-04-01 00:00:00pages
51-66issue
1eissn
0950-382Xissn
1365-2958pii
MMI5074journal_volume
60pub_type
杂志文章abstract：:Sigma28 controls the expression of flagella-related genes and is the most widely distributed alternative sigma factor, present in motile Gram-positive and Gram-negative bacteria. The distinguishing feature of sigma28 promoters is a long -10 region (GCCGATAA). Despite the fact that the upstream GC is highly conserved, ...
journal_title：Molecular microbiology
pub_type： 杂志文章
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journal_title：Molecular microbiology
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journal_title：Molecular microbiology
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doi：10.1111/j.1365-2958.2008.06402.x
更新日期：2008-10-01 00:00:00
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更新日期：2001-05-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2001-03-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1991.tb01858.x
更新日期：1991-12-01 00:00:00
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journal_title：Molecular microbiology
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更新日期：2011-07-01 00:00:00
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更新日期：2017-10-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2000.02040.x
更新日期：2000-08-01 00:00:00
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更新日期：2010-04-01 00:00:00
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更新日期：2008-01-01 00:00:00
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更新日期：2010-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1046/j.1365-2958.1996.00615.x
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pub_type： 杂志文章
doi：10.1111/mmi.14001
更新日期：2018-10-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
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更新日期：2011-09-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1995.tb02250.x
更新日期：1995-01-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 评论,杂志文章
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更新日期：2006-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：2005-11-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2009.06851.x
更新日期：2009-10-01 00:00:00
abstract：:An 18 633 bp region containing the replicon from the approximately 53 kb pBM400 plasmid of Bacillus megaterium QM B1551 has been sequenced and characterized. This region contained a complete rRNA operon plus 10 other potential open reading frames (ORFs). The replicon consisted of an upstream promoter and three contigu...
journal_title：Molecular microbiology
pub_type： 杂志文章
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更新日期：2001-02-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1990.tb00720.x
更新日期：1990-08-01 00:00:00
