Abstract：
:Trypanosoma cruzi, the etiologic agent of Chagas disease, has a complex life cycle in which four distinct developmental forms alternate between the insect vector and the mammalian host. It is assumed that replicating epimastigotes present in the insect gut are not infective to mammalian host, a paradigm corroborated by the widely acknowledged fact that only this stage is susceptible to the complement system. In the present work, we establish a T. cruzi in vitro and in vivo epimastigogenesis model to analyze the biological aspects of recently differentiated epimastigotes (rdEpi). We show that both trypomastigote stages of T. cruzi (cell-derived and metacyclic) are able to transform into epimastigotes (processes termed primary and secondary epimastigogenesis, respectively) and that rdEpi have striking properties in comparison to long-term cultured epimastigotes: resistance to complement-mediated lysis and both in vitro (cell culture) and in vivo (mouse) infectivity. Proteomics analysis of all T. cruzi stages reveled a cluster of proteins that were up-regulated only in rdEpi (including ABC transporters and ERO1), suggesting a role for them in rdEpi virulence. The present work introduces a new experimental model for the study of host-parasite interactions, showing that rdEpi can be infective to the mammalian host.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Kessler RL,Contreras VT,Marliére NP,Aparecida Guarneri A,Villamizar Silva LH,Mazzarotto GACA,Batista M,Soccol VT,Krieger MA,Probst CMdoi
10.1111/mmi.13653subject
Has Abstractpub_date
2017-06-01 00:00:00pages
712-736issue
5eissn
0950-382Xissn
1365-2958journal_volume
104pub_type
杂志文章abstract：:Amino acid sequence analyses have indicated that the amino-terminal part of bacteriophage PRD1 structural protein P7 carries a conserved transglycosylase domain. We analysed wild-type PRD1 and different mutant particles in zymograms and found a glycolytic activity that was associated with protein P7. This is the first...
journal_title：Molecular microbiology
pub_type： 杂志文章
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更新日期：2000-07-01 00:00:00
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更新日期：2020-10-04 00:00:00
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更新日期：2005-12-01 00:00:00
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更新日期：2006-09-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：1995-04-01 00:00:00
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pub_type： 杂志文章
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更新日期：1992-06-01 00:00:00
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更新日期：2007-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1046/j.1365-2958.1998.00858.x
更新日期：1998-06-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2010.07193.x
更新日期：2010-07-01 00:00:00
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doi：10.1111/j.1365-2958.2007.06015.x
更新日期：2007-12-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1994.tb00327.x
更新日期：1994-02-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2008.06567.x
更新日期：2009-02-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
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更新日期：2011-11-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1992.tb01496.x
更新日期：1992-02-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
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更新日期：2000-11-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.13988
更新日期：2018-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：1999-06-01 00:00:00
abstract：:The non-essential RGD1 gene from Saccharomyces cerevisiae encodes a protein that has been characterized in vitro as a Rho GTPase activating protein (RhoGAP) for the Rho3 and Rho4 proteins. Rgd1p, which displays a conserved FCH-coiled coil-Rho-GAP domain organization, showed a patch-like distribution in the cell, inclu...
journal_title：Molecular microbiology
pub_type： 杂志文章
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更新日期：2000-06-01 00:00:00
abstract：:The haemolysin of Haemophilus ducreyi is the newest member of the Proteus/Serratia family of pore-forming toxins. In order to assess the role of the haemolysin in virulence, we constructed an isogenic haemolysin-deficient mutant of H. ducreyi strain 35000 This strain, designated 35000-3, lacks detectable haemolytic ac...
journal_title：Molecular microbiology
pub_type： 杂志文章
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更新日期：1996-07-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
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更新日期：2007-07-01 00:00:00
abstract：:PspF bound to the psp enhancer activates E sigma54 holoenzyme-dependent transcription of the Escherichia coli phage-shock protein (psp) operon and autogenously represses its own sigma70-dependent transcription, thereby keeping its concentration at a low level. It has been demonstrated previously that integration host ...
journal_title：Molecular microbiology
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更新日期：1997-08-01 00:00:00
abstract：:Platelet binding by Streptococcus gordonii strain M99 is strongly correlated with the expression of the large surface glycoprotein GspB. A 14 kb chromosomal region downstream of gspB was previously shown to be required for the expression of this protein. The region encodes SecA2 and SecY2, which are components of an a...
journal_title：Molecular microbiology
pub_type： 杂志文章
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更新日期：2004-04-01 00:00:00
abstract：:Schizosaccharomyces pombe Rho1p regulates (1,3)beta-d-glucan synthesis and is required for cell integrity maintenance and actin cytoskeleton organization, but nothing is known about the regulation of this protein. At least nine different S. pombe genes code for proteins predicted to act as Rho GTPase-activating protei...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2003.03312.x
更新日期：2003-01-01 00:00:00
abstract：:Urinary tract infection (UTI) is a very common extraintestinal infection, and Escherichia coli is by far the most common causative organism. Uropathogenic E. coli possess traits that distinguish them from commensal strains of E. coli, such as secretion systems that allow virulence factors to be targeted to extracytopl...
journal_title：Molecular microbiology
pub_type： 杂志文章
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更新日期：2000-10-01 00:00:00
abstract：:The recombination of short homologous ends in Escherichia coli has been known for 30 years, and it is often used for both site-directed mutagenesis and in vivo cloning. For cloning, a plasmid and target DNA fragments were converted into linear DNA fragments with short homologous ends, which are joined via recombinatio...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.14677
更新日期：2020-12-29 00:00:00
