Combining reverse genetics and nuclear magnetic resonance-based metabolomics unravels trypanosome-specific metabolic pathways.

abstract：:In Escherichia coli, initiation of chromosome replication requires that DnaA binds to R boxes (9-mer repeats) in oriC, the unique chromosomal replication origin. At the time of initiation, integration host factor (IHF) also binds to a specific site in oriC. IHF stimulates open complex formation by DnaA on supercoiled ...

journal_title：Molecular microbiology

authors： Grimwade JE,Ryan VT,Leonard AC

更新日期：2000-02-01 00:00:00

abstract：:Pathogenic enterotoxigenic Escherichia coli (ETEC) are the major bacterial cause of diarrhea in young children in developing countries and in travelers, causing significant mortality in children. Adhesive fimbriae are a prime virulence factor for ETEC, initiating colonization of the small intestinal epithelium. Simila...

journal_title：Molecular microbiology

authors： Mortezaei N,Epler CR,Shao PP,Shirdel M,Singh B,McVeigh A,Uhlin BE,Savarino SJ,Andersson M,Bullitt E

更新日期：2015-01-01 00:00:00

abstract：:Deletions or substitutions of amino acids at the carboxyl-terminus of the heat-labile enterotoxin B subunit (EtxB) affect its assembly into pentamers in a temperature-dependent manner. At 42 degrees C, the mutations prevent the B subunits from achieving their final pentameric structure resulting in membrane associatio...

journal_title：Molecular microbiology

authors： Sandkvist M,Bagdasarian M

更新日期：1993-11-01 00:00:00

abstract：:Salmonella Typhimurium causes bacterial enterocolitis. The type III secretion system (TTSS)-1 is a key virulence determinant of S. Typhimurium mediating host cell invasion and acute enterocolitis. The TTSS-1 effector protein SipA is transported into host cells, accumulates in characteristic foci at the bacteria-host c...

journal_title：Molecular microbiology

authors： Schlumberger MC,Käppeli R,Wetter M,Müller AJ,Misselwitz B,Dilling S,Kremer M,Hardt WD

更新日期：2007-08-01 00:00:00

abstract：:The ferric uptake regulator (Fur) protein is known to act as a Fe2+-dependent transcriptional repressor of bacterial promoters. Here, we show that, in Helicobacter pylori, Fur can mediate the regulation of iron-activated genes in contrast to classical Fur regulation, in which iron acts as a co-repressor. Inactivation ...

journal_title：Molecular microbiology

authors： Delany I,Spohn G,Rappuoli R,Scarlato V

更新日期：2001-12-01 00:00:00

abstract：:Ail, a multifunctional outer membrane protein of Yersinia pestis, confers cell binding, Yop delivery and serum resistance activities. Resistance to complement proteins in serum is critical for the survival of Y. pestis during the septicemic stage of plague infections. Bacteria employ a variety of tactics to evade the ...

journal_title：Molecular microbiology

authors： Thomson JJ,Plecha SC,Krukonis ES

更新日期：2019-01-01 00:00:00

abstract：:The mechanistic details of the pathogenesis of Chlamydia, an obligate intracellular pathogen of global importance, have eluded scientists due to the scarcity of traditional molecular genetic tools to investigate this organism. Here we report a chemical biology strategy that has uncovered the first essential protease f...

journal_title：Molecular microbiology

authors： Gloeckl S,Ong VA,Patel P,Tyndall JD,Timms P,Beagley KW,Allan JA,Armitage CW,Turnbull L,Whitchurch CB,Merdanovic M,Ehrmann M,Powers JC,Oleksyszyn J,Verdoes M,Bogyo M,Huston WM

更新日期：2013-08-01 00:00:00

abstract：:Streptococcus pyogenes interacts with host fibronectin via distinct surface components. One of these components is the Sfbl protein (streptococcal fibronectin-binding protein, now specified as class I), an adhesin that represents a protein family with characteristic features. Here we present the complete structure of ...

journal_title：Molecular microbiology

authors： Kreikemeyer B,Talay SR,Chhatwal GS

更新日期：1995-07-01 00:00:00

abstract：:A respiratory quinol oxidase complex that is encoded by the soxABCD operon has been purified from the thermoacidophilic archaeon Sulfolobus acidocaldarius. The enzyme was solubilized with dodecyl maltoside and purified in the presence of this detergent and ethylene glycol. The complex is hydrodynamically homogeneous a...

journal_title：Molecular microbiology

authors： Lübben M,Warne A,Albracht SP,Saraste M

更新日期：1994-07-01 00:00:00

abstract：:The nucleotide sequence of a 2711bp DNA segment which contains the N-terminal coding sequence and the 5' flanking region of a crystal protein gene (bta) from Bacillus thuringiensis subsp. aizawai 7.29 has been determined. The coding region encodes an 824 amino-acid polypeptide corresponding to a carboxy-terminally tru...

journal_title：Molecular microbiology

authors： Sanchis V,Lereclus D,Menou G,Chaufaux J,Guo S,Lecadet MM

更新日期：1989-02-01 00:00:00

abstract：:The largest subunit of RNA polymerase II, Rpb1, contains an unusual C-terminal domain (CTD) composed of numerous repeats of the YSPTSPS consensus sequence. This sequence is the target of post-translational modifications such as phosphorylation, glycosylation, methylation and transitions between stereoisomeric states, ...

journal_title：Molecular microbiology

authors： Drogat J,Hermand D

更新日期：2012-06-01 00:00:00

abstract：:Rhamnolipid acts as a virulence factor during Pseudomonas aeruginosa infection. Here, we show that deletion of the catabolite repression control (crc) gene in P. aeruginosa leads to a rhamnolipid-negative phenotype. This effect is mediated by the down-regulation of rhl quorum sensing (QS). We discover that a disruptio...

journal_title：Molecular microbiology

authors： Yang N,Ding S,Chen F,Zhang X,Xia Y,Di H,Cao Q,Deng X,Wu M,Wong CC,Tian XX,Yang CG,Zhao J,Lan L

更新日期：2015-05-01 00:00:00

abstract：:In almost all bacteria, cell division is co-ordinated by the essential tubulin homologue FtsZ and represents an attractive but as yet unexploited target for new antibiotics. The benzamides, e.g. PC190723, are potent FtsZ inhibitors that have the potential to yield an important new class of antibiotic. However, the evo...

journal_title：Molecular microbiology

authors： Adams DW,Wu LJ,Errington J

更新日期：2016-03-01 00:00:00

abstract：:Expression of the Aspergillus nidulans 22 kDa endoxylanase gene, xlnA, is controlled by at least three mechanisms: specific induction by xylan or xylose; carbon catabolite repression (CCR); and regulation by ambient pH. Deletion analysis of xlnA upstream sequences has identified two positively acting regions: one that...

journal_title：Molecular microbiology

authors： Orejas M,MacCabe AP,Pérez González JA,Kumar S,Ramón D

更新日期：1999-01-01 00:00:00

abstract：:This review assembles data from three bodies of literature (bacterial genetics, plastid biogenesis and parasitology) that seldom have much direct cross-talk. After overcoming terminological complications to sort out microbial nifS from sufS genes, we connect a bacterial operon, recently found to be involved in iron me...

journal_title：Molecular microbiology

authors： Ellis KE,Clough B,Saldanha JW,Wilson RJ

更新日期：2001-09-01 00:00:00

abstract：:The presence of very different sets of enzymes, and in particular the presence of RNase E and RNase J, has been used to explain significant differences in RNA metabolism between the two model organisms Escherichia coli and Bacillus subtilis. However, these studies might have somewhat polarized our view of RNA metaboli...

journal_title：Molecular microbiology

authors： Taverniti V,Forti F,Ghisotti D,Putzer H

更新日期：2011-12-01 00:00:00

abstract：:The utilization of several sugars in Escherichia coli is regulated by the Phosphotransferase System (PTS), in which diverse sugar utilization modules compete for phosphoryl flux from the general PTS proteins. Existing theoretical work predicts a winner-take-all outcome when this flux limits carbon uptake. To date, no ...

journal_title：Molecular microbiology

authors： Westermayer SA,Fritz G,Gutiérrez J,Megerle JA,Weißl MP,Schnetz K,Gerland U,Rädler JO

更新日期：2016-05-01 00:00:00

abstract：:Nitrogen metabolism genes of Bacillus subtilis are regulated by the availability of rapidly metabolizable nitrogen sources, but not by any mechanism analogous to the two-component Ntr regulatory system found in enteric bacteria. Instead, at least three regulatory proteins independently control the expression of gene p...

journal_title：Molecular microbiology

authors： Fisher SH

更新日期：1999-04-01 00:00:00

abstract：:Virulence factors secreted by Listeria monocytogenes are known to interfere with host cellular signalling pathways. We investigated whether L. monocytogenes modulates T-cell receptor signalling by examining surface expression of proteins known to be upregulated on activated T cells. In vitro culture of murine splenocy...

journal_title：Molecular microbiology

authors： Zenewicz LA,Skinner JA,Goldfine H,Shen H

更新日期：2004-03-01 00:00:00

abstract：:Phospholipases C play a role in the pathogenesis of several bacteria. Mycobacterium tuberculosis, the causative agent of tuberculosis, possesses four genes encoding putative phospholipases C, plcA, plcB, plcC and plcD. However, the contribution of these genes to virulence is unknown. We constructed four single mutants...

journal_title：Molecular microbiology

authors： Raynaud C,Guilhot C,Rauzier J,Bordat Y,Pelicic V,Manganelli R,Smith I,Gicquel B,Jackson M

更新日期：2002-07-01 00:00:00

abstract：:Metal ions are crucial trace elements for bacteria infecting the human host. The LraI (lipoprotein receptor-associated antigen I) transporter in Streptococcus spp. belongs to the superfamily of ABC transporters. The transporter consists of a lipoprotein, an ATP-binding protein and a hydrophobic integral membrane prote...

journal_title：Molecular microbiology

authors： Janulczyk R,Pallon J,Björck L

更新日期：1999-11-01 00:00:00

abstract：:Pseudomonas syringae pv. tomato (Pst) strain DC3000 infects the model plants Arabidopsis thaliana and tomato, causing disease symptoms characterized by necrotic lesions surrounded by chlorosis. One mechanism used by Pst DC3000 to infect host plants is the type III protein secretion system, which is thought to deliver ...

journal_title：Molecular microbiology

authors： Zwiesler-Vollick J,Plovanich-Jones AE,Nomura K,Bandyopadhyay S,Joardar V,Kunkel BN,He SY

更新日期：2002-09-01 00:00:00

abstract：:The importance of methylthioadenosine/S-adenosylhomocysteine (MTA/SAH) nucleosidase in bacteria has started to be appreciated only in the past decade. A comprehensive analysis of its various roles here demonstrates that it is an integral component of the activated methyl cycle, which recycles adenine and methionine th...

journal_title：Molecular microbiology

authors： Parveen N,Cornell KA

更新日期：2011-01-01 00:00:00

abstract：:We have built a homology model of the AAA domain of the ATP-dependent protease FtsH of Escherichia coli based on the crystal structure of the hexamerization domain of N-ethylmaleimide-sensitive fusion protein. The resulting model of the hexameric ring of the ATP-bound form of the AAA ATPase suggests a plausible mechan...

journal_title：Molecular microbiology

authors： Karata K,Verma CS,Wilkinson AJ,Ogura T

更新日期：2001-02-01 00:00:00

abstract：:A search was performed for a periplasmic molecular chaperone which may assist outer membrane proteins of Escherichia coli on their way from the cytoplasmic to the outer membrane. Proteins of the periplasmic space were fractionated on an affinity column with sepharose-bound outer membrane porin OmpF. A 17 kDa polypepti...

journal_title：Molecular microbiology

authors： Chen R,Henning U

更新日期：1996-03-01 00:00:00

abstract：:The monocistronic transcript of rpsO undergoes an endonucleolytic cleavage downstream of the coding sequence, which removes the hairpin of the transcription terminator and initiates the rapid degradation of the message. We demonstrate here that the two rne-dependent cleavages, on both sides of the transcription termin...

journal_title：Molecular microbiology

authors： Braun F,Hajnsdorf E,Régnier P

更新日期：1996-03-01 00:00:00

abstract：:The expression of the structural genes for lysine (LYS) biosynthesis is controlled by a pathway-specific regulation mediated by the transcriptional activator Lys14 in the presence of alpha-aminoadipate semialdehyde, an intermediate of the pathway acting as a co-inducer. Owing to end product inhibition of the first ste...

journal_title：Molecular microbiology

authors： Becker B,Feller A,el Alami M,Dubois E,Piérard A

更新日期：1998-07-01 00:00:00

abstract：:The natural resistance of Mycobacterium abscessus to most commonly available antibiotics seriously limits chemotherapeutic treatment options, which is particularly challenging for cystic fibrosis patients infected with this rapid-growing mycobacterium. New drugs with novel molecular targets are urgently needed against...

journal_title：Molecular microbiology

authors： Dupont C,Viljoen A,Dubar F,Blaise M,Bernut A,Pawlik A,Bouchier C,Brosch R,Guérardel Y,Lelièvre J,Ballell L,Herrmann JL,Biot C,Kremer L

更新日期：2016-08-01 00:00:00

abstract：:The opportunistic pathogen Pseudomonas aeruginosa produces type 4 fimbriae which promote adhesion to epithelial cells and are associated with a form of surface translocation called twitching motility. Transposon mutagenesis was used to identify loci required for fimbrial assembly or function by screening for mutants t...

journal_title：Molecular microbiology

authors： Martin PR,Watson AA,McCaul TF,Mattick JS

更新日期：1995-05-01 00:00:00

abstract：:The DnaA protein of Escherichia coli is a multi-functional protein which, In addition to promoting initiation of replication, can regulate the initiation or termination of transcription of a variety of genes. It acts by binding to DNA at a defined sequence, termed a DnaA-box. Three candidate DnaA-boxes which occur wit...

journal_title：Molecular microbiology

authors： Smith RW,McAteer S,Masters M

更新日期：1996-07-01 00:00:00