Combining reverse genetics and nuclear magnetic resonance-based metabolomics unravels trypanosome-specific metabolic pathways.


:Numerous eukaryotes have developed specific metabolic traits that are not present in extensively studied model organisms. For instance, the procyclic insect form of Trypanosoma brucei, a parasite responsible for sleeping sickness in its mammalian-specific bloodstream form, metabolizes glucose into excreted succinate and acetate through pathways with unique features. Succinate is primarily produced from glucose-derived phosphoenolpyruvate in peroxisome-like organelles, also known as glycosomes, by a soluble NADH-dependent fumarate reductase only described in trypanosomes so far. Acetate is produced in the mitochondrion of the parasite from acetyl-CoA by a CoA-transferase, which forms an ATP-producing cycle with succinyl-CoA synthetase. The role of this cycle in ATP production was recently demonstrated in procyclic trypanosomes and has only been proposed so far for anaerobic organisms, in addition to trypanosomatids. We review how nuclear magnetic resonance spectrometry can be used to analyze the metabolic network perturbed by deletion (knockout) or downregulation (RNAi) of the candidate genes involved in these two particular metabolic pathways of procyclic trypanosomes. The role of succinate and acetate production in trypanosomes is discussed, as well as the connections between the succinate and acetate branches, which increase the metabolic flexibility probably required by the parasite to deal with environmental changes such as oxidative stress.


Mol Microbiol


Molecular microbiology


Bringaud F,Biran M,Millerioux Y,Wargnies M,Allmann S,Mazet M




Has Abstract


2015-06-01 00:00:00












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    abstract::The presence of capsular exopolysaccharide (EPS) in Mollicutes has been inferred from electron micrographs for over 50 years without conclusive data to support the production of complex carbohydrates by the organism. Mycoplasma pulmonis binds the lectin Griffonia simplicifolia I (GS-I), which is specific for terminal ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Daubenspeck JM,Bolland JR,Luo W,Simmons WL,Dybvig K

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  • Molecular surveillance of the subtle septum: discovering a new mode of peptidoglycan synthesis in streptococci.

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    journal_title:Molecular microbiology

    pub_type: 评论,杂志文章


    authors: Cadby IT,Lovering AL

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    abstract::A method called Muprinting has been developed that uses PCR to generate a detailed picture of the bacteriophage Mu transposition sites in chosen domains of the bacterial chromosome. Muprinting experiments in Escherichia coli show that the frequency of phage integration changes dramatically near two repressor binding s...

    journal_title:Molecular microbiology

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    authors: Wang X,Higgins NP

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  • The ObgE/CgtA GTPase influences the stringent response to amino acid starvation in Escherichia coli.

    abstract::The stringent response is important for bacterial survival under stressful conditions, such as amino acid starvation, and is characterized by the accumulation of ppGpp and pppGpp. ObgE (CgtA, YhbZ) is an essential conserved GTPase in Escherichia coli and several observations have implicated the protein in the control ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


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    abstract::Regulation of polar development and cell division in Caulobacter crescentus relies on the dynamic localization of several proteins to cell poles at specific stages of the cell cycle. The polar organelle development protein, PodJ, is required for the synthesis of the adhesive holdfast and pili. Here we show the cell cy...

    journal_title:Molecular microbiology

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    journal_title:Molecular microbiology

    pub_type: 评论,杂志文章


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    journal_title:Molecular microbiology

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    pub_type: 杂志文章


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    authors: Roberts SA,Scott JR

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Li X,Lockatell CV,Johnson DE,Mobley HL

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Follettie MT,Shin HK,Sinskey AJ

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Podbielski A,Leonard BA

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    abstract::Type II restriction enzymes are paired with modification enzymes that protect type II restriction sites from cleavage by methylating them. A plasmid carrying a type II restriction-modification gene complex is not easily replaced by an incompatible plasmid because loss of the former leads to cell death through chromoso...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Handa N,Nakayama Y,Sadykov M,Kobayashi I

    更新日期:2001-05-01 00:00:00

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    abstract::PrkC was shown to be a eukaryotic-like (Hanks-type) protein kinase from Bacillus subtilis with a structural organization similar to that of the eukaryotic sensor Ser/Thr or Tyr kinases (e.g. the TGF beta or PDGF receptors). The molecule consists of a catalytic domain located in the cytoplasm, joined by a single transm...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Madec E,Laszkiewicz A,Iwanicki A,Obuchowski M,Séror S

    更新日期:2002-10-01 00:00:00

  • Agrobacterium tumefaciens oncogenic suppressors inhibit T-DNA and VirE2 protein substrate binding to the VirD4 coupling protein.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Cascales E,Atmakuri K,Liu Z,Binns AN,Christie PJ

    更新日期:2005-10-01 00:00:00

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Gostick DO,Griffin HG,Shearman CA,Scott C,Green J,Gasson MJ,Guest JR

    更新日期:1999-03-01 00:00:00

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Safi H,Barnes PF,Lakey DL,Shams H,Samten B,Vankayalapati R,Howard ST

    更新日期:2004-05-01 00:00:00