Identification of an HP1 phage protein required for site-specific excision.


:Transposon insertion mutagenesis and transformation were used to locate genes responsible for excision in the temperature phage HP1 of Haemophilus influenzae. A 6.5 kb segment of DNA near the left end of the phage genome was sequenced, and 11 new open reading frames were identified. Two face-to-face overlapping promoter sequences organized these open reading frames into two operons transcribed in opposite directions. Interruption of the first open reading frame in the rightward operon created lysogens unable to produce phages. Provision of the uninterrupted open reading frame in trans restored phage production. The gene identified by this procedure, cox, was cloned and the protein product was expressed at high levels in Escherichia coli. The Cox protein is a 79-residue basic protein with a predicted strong helix-turn-helix DNA-binding motif. Extracts induced to express high levels of Cox contained a 9 kDa protein. These extracts inhibited integrative recombination and were required for excisive recombination mediated by HP1 integrase. The HP1 cox gene location is similar to that of the homologous excisive and regulatory genes from coliphages P2 and 186. These phages appear to share a distinctive organization of recombination proteins and transcriptional domains differing markedly from phage lambda and its relatives.


Mol Microbiol


Molecular microbiology


Esposito D,Scocca JJ




Has Abstract


1994-08-01 00:00:00












  • Mutational and physical analysis of F plasmid traY protein binding to oriT.

    abstract::F plasmid traY protein binding to wild-type or deleted regions containing the TraY-binding site, sbyA, was studied in vitro. The principal DNA-protein complex was formed with DNA segments including the sbyA site defined by footprinting and (with lesser affinity) with truncated segments that retained the leftward two-t...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Luo Y,Gao Q,Deonier RC

    更新日期:1994-02-01 00:00:00

  • Overexpression and topology of the Shigella flexneri O-antigen polymerase (Rfc/Wzy).

    abstract::Lipopolysaccharides (LPS), particularly the O-antigen component, are one of many virulence determinants necessary for Shigella flexneri pathogenesis. O-antigen biosynthesis is determined mostly by genes located in the rfb region of the chromosome. The rfc/wzy gene encodes the O-antigen polymerase, an integral membrane...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Daniels C,Vindurampulle C,Morona R

    更新日期:1998-06-01 00:00:00

  • Structural insights into the inhibition mechanism of bacterial toxin LsoA by bacteriophage antitoxin Dmd.

    abstract::Bacteria have obtained a variety of resistance mechanisms including toxin-antitoxin (TA) systems against bacteriophages (phages), whereas phages have also evolved to overcome bacterial anti-phage mechanisms. Dmd from T4 phage can suppress the toxicities of homologous toxins LsoA and RnlA from Escherichia coli, represe...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Wan H,Otsuka Y,Gao ZQ,Wei Y,Chen Z,Masuda M,Yonesaki T,Zhang H,Dong YH

    更新日期:2016-09-01 00:00:00

  • Molecular analysis of a microaerobically induced operon required for hydrogenase synthesis in Rhizobium leguminosarum biovar viciae.

    abstract::The nucleotide sequence (6138 bp) of a microaerobically inducible region (hupV/VI) from the Rhizobium leguminosarum bv. viciae hydrogenase gene cluster has been determined. Six genes, arranged as a single operon, were identified, and designated hypA, B, F, C, D and E based on the sequence similarities of all of them, ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Rey L,Murillo J,Hernando Y,Hidalgo E,Cabrera E,Imperial J,Ruiz-Argüeso T

    更新日期:1993-05-01 00:00:00

  • Calcineurin responsive zinc-finger-1 binds to a unique promoter sequence to upregulate neuronal calcium sensor-1, whose interaction with MID-1 increases tolerance to calcium stress in Neurospora crassa.

    abstract::We studied the molecular mechanism of neuronal calcium sensor-1 (NCS-1) signaling pathway for tolerance to Ca2+ stress in Neurospora crassa. Increasing concentration of Ca2+ increased the expression of ncs-1; however, the calcineurin inhibitor FK506 severely reduced ncs-1 mRNA transcript levels. Chromatin immunoprecip...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Gohain D,Tamuli R

    更新日期:2019-06-01 00:00:00

  • Entamoeba histolytica: electron-dense granule secretion, collagenase activity and virulence are altered in the cytoskeleton mutant BG-3.

    abstract::HM-1:IMSS, a pathogenic strain of Entamoeba histolytica, and its mutant BG-3, identified by resistance to cytochalasin D, were tested for their capacity to: (i) secrete electron-dense granules; (ii) adhere and digest native type I collagen gels; and (iii) produce liver abscesses in new-born hamsters. The results demon...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Serrano JJ,de la Garza M,Moreno MA,Tovar R,León G,Tsutsumi V,Muñoz ML

    更新日期:1994-02-01 00:00:00

  • Iron and copper as virulence modulators in human fungal pathogens.

    abstract::Fungal pathogens have evolved sophisticated machinery to precisely balance the fine line between acquiring essential metals and defending against metal toxicity. Iron and copper are essential metals for many processes in both fungal pathogens and their mammalian hosts, but reduce viability when present in excess. Howe...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


    authors: Ding C,Festa RA,Sun TS,Wang ZY

    更新日期:2014-07-01 00:00:00

  • Metabolic adaptation in Cryptococcus neoformans during early murine pulmonary infection.

    abstract:SUMMARY:The pathogenic fungus Cryptococcus neoformans generally initiates infection in mammalian lung tissue and subsequently disseminates to the brain. We performed serial analysis of gene expression (SAGE) on C. neoformans cells recovered from the lungs of mice and found elevated expression of genes for central carbo...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Hu G,Cheng PY,Sham A,Perfect JR,Kronstad JW

    更新日期:2008-09-01 00:00:00

  • Comparative characterization of the iga gene encoding IgA1 protease in Neisseria meningitidis, Neisseria gonorrhoeae and Haemophilus influenzae.

    abstract::Cloning and sequencing of the IgA1 protease gene (iga) from Neisseria meningitidis strain HF13 showed an overall structure equivalent to iga genes from Neisseria gonorrhoeae and Haemophilus influenzae, although no region corresponding to the gonococcal alpha-peptide was evident. An additional 18 N. meningitidis and 3 ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Lomholt H,Poulsen K,Kilian M

    更新日期:1995-02-01 00:00:00

  • The FNR-type transcriptional regulator SinR controls maturation of Agrobacterium tumefaciens biofilms.

    abstract::Agrobacterium tumefaciens is a plant pathogen that persists as surface-associated populations on plants or soil particles. A genetic screen for A. tumefaciens mutants deficient for surface interactions identified a mutant that forms thin, sparsely populated biofilms, but is proficient for initial attachment. The mutan...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Ramey BE,Matthysse AG,Fuqua C

    更新日期:2004-06-01 00:00:00

  • MexAB-OprM hyperexpression in NalC-type multidrug-resistant Pseudomonas aeruginosa: identification and characterization of the nalC gene encoding a repressor of PA3720-PA3719.

    abstract::MexAB-OprM is a multidrug efflux system that contributes to intrinsic and acquired multidrug resistance in Pseudomonas aeruginosa, the latter as a result of mutational hyperexpression of the mexAB-oprM operon. While efflux gene hyperexpression typically results from mutations in the linked mexR repressor gene, it also...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Cao L,Srikumar R,Poole K

    更新日期:2004-09-01 00:00:00

  • Microtubule-severing proteins are involved in flagellar length control and mitosis in Trypanosomatids.

    abstract::Microtubules are key players in the biology of Trypanosomatid parasites, not only as classical components of the mitotic spindle, microtubule-organizing centres and flagellum but also as the essential constituent of the cytoskeleton. Their length dynamics are regulated by, among others, microtubule-severing proteins. ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Casanova M,Crobu L,Blaineau C,Bourgeois N,Bastien P,Pagès M

    更新日期:2009-03-01 00:00:00

  • Evidence for a rolling-circle mechanism of phage DNA synthesis from both replicative and integrated forms of CTXphi.

    abstract::The genes encoding cholera toxin, the principal virulence factor of Vibrio cholerae, are part of the circular single-stranded DNA genome of CTXphi. In toxigenic V. cholerae strains, the CTXphi genome is typically found in integrated arrays of tandemly arranged CTX prophages. Infected cells that lack a chromosomal inte...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Moyer KE,Kimsey HH,Waldor MK

    更新日期:2001-07-01 00:00:00

  • Structural, genetic and functional characterization of the flagellin glycosylation process in Helicobacter pylori.

    abstract::Mass spectrometry analyses of the complex polar flagella from Helicobacter pylori demonstrated that both FlaA and FlaB proteins are post-translationally modified with pseudaminic acid (Pse5Ac7Ac, 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno -n o n-ulosonic acid). Unlike Campylobacter, flagellar glycosylation i...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Schirm M,Soo EC,Aubry AJ,Austin J,Thibault P,Logan SM

    更新日期:2003-06-01 00:00:00

  • Visualization of the subcellular location of sporulation proteins in Bacillus subtilis using immunofluorescence microscopy.

    abstract::We describe the application of immunofluorescence microscopy to visualization of the subcellular localization of proteins involved in coat morphogenesis and chromosome packaging during the process of sporulation in Bacillus subtilis. In confirmation and extension of previous findings, we show that SpolVA, which is res...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Pogliano K,Harry E,Losick R

    更新日期:1995-11-01 00:00:00

  • Probing the structure-activity relationship of Escherichia coli LT-A by site-directed mutagenesis.

    abstract::Computer analysis of the crystallographic structure of the A subunit of Escherichia coli heat-labile toxin (LT) was used to predict residues involved in NAD binding, catalysis and toxicity. Following site-directed mutagenesis, the mutants obtained could be divided into three groups. The first group contained fully ass...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Pizza M,Domenighini M,Hol W,Giannelli V,Fontana MR,Giuliani MM,Magagnoli C,Peppoloni S,Manetti R,Rappuoli R

    更新日期:1994-10-01 00:00:00

  • Regulation of glutamate dehydrogenase expression in Pseudomonas putida results from its direct repression by NtrC under nitrogen-limiting conditions.

    abstract::Nitrogen-regulated genes in enterobacteria are positively controlled by the transcriptional activator of σ(N) -dependent promoters NtrC, either directly or indirectly, through the dual regulator Nac. Similar to enterobacteria, gdhA encoding glutamate dehydrogenase from Pseudomonas putida is one of the few genes that i...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Hervás AB,Canosa I,Santero E

    更新日期:2010-10-01 00:00:00

  • Differential RNA elongation controls the variant surface glycoprotein gene expression sites of Trypanosoma brucei.

    abstract::The protozoan parasite Trypanosoma brucei develops antigenic variation to escape the immune response of its host. To this end, the trypanosome genome contains multiple telomeric expression sites competent for transcription of variant surface glycoprotein genes, but as a rule only a single antigen is expressed at any t...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Vanhamme L,Poelvoorde P,Pays A,Tebabi P,Van Xong H,Pays E

    更新日期:2000-04-01 00:00:00

  • Cell wall-inhibitory antibiotics activate the alginate biosynthesis operon in Pseudomonas aeruginosa: Roles of sigma (AlgT) and the AlgW and Prc proteases.

    abstract::A bioassay was developed to identify stimuli that promote the transcriptional induction of the algD operon for alginate biosynthesis in Pseudomonas aeruginosa. Strain PAO1 carried the algD promoter fused to a chloramphenicol acetyl-transferase cartridge (PalgD-cat), and > 50 compounds were tested for promoting chloram...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Wood LF,Leech AJ,Ohman DE

    更新日期:2006-10-01 00:00:00

  • Hop-family Helicobacter outer membrane adhesins form a novel class of Type 5-like secretion proteins with an interrupted β-barrel domain.

    abstract::The human stomach pathogen Helicobacter pyloriattaches to healthy and inflamed gastric tissue through members of a paralogous family of 'Helicobacter outer membrane proteins' (Hops), including adhesins BabA, SabA, HopQ, LabA and HopZ. Hops share a conserved 25 kDa C-terminal region that is thought to form an autotrans...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Coppens F,Castaldo G,Debraekeleer A,Subedi S,Moonens K,Lo A,Remaut H

    更新日期:2018-10-01 00:00:00

  • Transmembrane signal transduction by the Escherichia coli osmotic sensor, EnvZ: intermolecular complementation of transmembrane signalling.

    abstract::The Escherichia coli regulatory proteins, EnvZ and OmpR, are crucially involved in expression of the outer membrane proteins OmpF/OmpC in response to the medium osmolarity. The EnvZ protein is presumably a membrane-located osmotic sensor (or signal transducer), which exhibits both kinase and phosphatase activities spe...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Tokishita S,Mizuno T

    更新日期:1994-08-01 00:00:00

  • Genetic identification of exported proteins in Streptococcus pneumoniae.

    abstract::A strategy was developed to mutate and genetically identify exported proteins in Streptococcus pneumoniae. Vectors were created and used to screen pneumococcal DNA in Escherichia coli and S. pneumoniae for translational gene fusions to alkaline phosphatase (PhoA). Twenty five PhoA+ pneumococcal mutants were isolated a...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Pearce BJ,Yin YB,Masure HR

    更新日期:1993-09-01 00:00:00

  • The essential roles of cytidine diphosphate-diacylglycerol synthase in bloodstream form Trypanosoma brucei.

    abstract::Lipid metabolism in Trypanosoma brucei, the causative agent of African sleeping sickness, differs from its human host in several fundamental ways. This has lead to the validation of a plethora of novel drug targets, giving hope of novel chemical intervention against this neglected disease. Cytidine diphosphate diacylg...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Lilley AC,Major L,Young S,Stark MJ,Smith TK

    更新日期:2014-05-01 00:00:00

  • The transcriptional organization of the Bacillus subtilis 168 chromosome region between the spoVAF and serA genetic loci.

    abstract::The genetic organization of the spoVAF-serA area of the Bacillus subtilis chromosome and its putative transcription map have been derived from analysis of the nucleotide sequence. In order to confirm this transcription map as regards size of transcripts and to determine growth conditions for their appearance, we under...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Azevedo V,Sorokin A,Ehrlich SD,Serror P

    更新日期:1993-10-01 00:00:00

  • Cloning and characterization of a relA/spoT homologue from Bacillus subtilis.

    abstract::A PCR-amplified DNA fragment of the relA gene from genomic Bacillus subtilis DNA was used to isolate the entire relA/spoT homologue and two adjacent open reading frames (ORFs) from a lambda ZAP Express library. The relA gene, which encodes a protein of 734 amino acid residues (aa), is flanked by an ORF (170aa) that sh...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Wendrich TM,Marahiel MA

    更新日期:1997-10-01 00:00:00

  • Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation.

    abstract::mscL encodes a channel in Escherichia coli that is opened by membrane stretch force, probably serving as an osmotic gauge. Sequences more or less similar to mscL are found in other bacteria, but the degree of conserved function has been unclear. We subcloned and expressed these putative homologues in E. coli and exami...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Moe PC,Blount P,Kung C

    更新日期:1998-05-01 00:00:00

  • Reduced polysialic acid capsule expression in Escherichia coli K1 mutants with chromosomal defects in kpsF.

    abstract::Neuroinvasive Escherichia coli K1 synthesizes and assembles a polysialic acid capsule virulence factor on the external leaflet of the outer membrane. This capsule functions in pathogenesis by blocking non-immune host defence mechanisms and acting as a relatively non-immunogenic molecular mimic of the polysialic acid c...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Cieslewicz M,Vimr E

    更新日期:1997-10-01 00:00:00

  • Sense and antisense transcripts of traM, a conjugal transfer gene of the antibiotic resistance plasmid R100.

    abstract::The region of the antibiotic resistance plasmid R100 that encodes the plasmid-specific transfer gene traM has two tandemly aligned promoters separated by 145 nucleotides. The principal transcripts are 705 and 562 nucleotides long. Minor transcripts are 1550 and 1700 nucleotides long. The 705-base transcript appears to...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Dempsey WB

    更新日期:1989-04-01 00:00:00

  • Consequences of RNase E scarcity in Escherichia coli.

    abstract::The endoribonuclease RNase E plays an important role in RNA processing and degradation in Escherichia coli. The construction of an E. coli strain in which the cellular concentration of RNase E can be precisely controlled has made it possible to examine and quantify the effect of RNase E scarcity on RNA decay, gene reg...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Jain C,Deana A,Belasco JG

    更新日期:2002-02-01 00:00:00

  • New insights into the protective effect of manganese against oxidative stress.

    abstract::Manganese has emerged as an important trace element in bacterial physiology. The correlation between manganese accumulation and resistance to oxidative stress has led to the suggestion that, in addition to a role as a prosthetic group in superoxide dismutase, manganese could exert its antioxidant effect via non-enzyma...

    journal_title:Molecular microbiology

    pub_type: 评论,杂志文章


    authors: McEwan AG

    更新日期:2009-05-01 00:00:00