A network of net-workers: report of the Euresco conference on 'Bacterial Neural Networks' held at San Feliu (Spain) from 8 to 14 May 2004.


:In May 2004, over 100 bacteriologists from 19 different countries discussed recent progress in identification and understanding of individual signal transfer mechanisms in bacteria and in the mutual interactions between these systems to form a functional living cell. The meeting was held in San Feliu and supported by ESF and EMBO. In part through the extensive sequencing efforts of the past few years, the bulk of the bacterial signal transfer systems have been resolved and their detailed characterization is revealing such characteristics as signal specificity, signalling rate constants, molecular interaction affinities, subcellular localization, etc., which should provide a solid basis to a computational extension of this field of studies. In parallel, the new genomics techniques are providing tools to characterize the way a collection of such systems interact in an individual cell, to give rise to 'life'. Systems theory provides rational and convenient ways to bring order to the wide range of observables thus obtained. Ultimately, the performance of engineered design will have to prove whether or not we know enough about the processes involved.


Mol Microbiol


Molecular microbiology


Hellingwerf KJ




Has Abstract


2004-10-01 00:00:00














  • Characterization of a novel Neisseria meningitidis Fur and iron-regulated operon required for protection from oxidative stress: utility of DNA microarray in the assignment of the biological role of hypothetical genes.

    abstract::We have previously shown that in the human pathogen Neisseria meningitidis group B (MenB) more than 200 genes are regulated in response to growth with iron. Among the Fur-dependent, upregulated genes identified by microarray analysis was a putative operon constituted by three genes, annotated as NMB1436, NMB1437 and N...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Grifantini R,Frigimelica E,Delany I,Bartolini E,Giovinazzi S,Balloni S,Agarwal S,Galli G,Genco C,Grandi G

    更新日期:2004-11-01 00:00:00

  • Alkaline shock induces the Bacillus subtilis sigma(W) regulon.

    abstract::When confronted with a stress factor, bacteria react with a specific stress response, a genetically encoded programme resulting in the transiently enhanced expression of a subset of genes. One of these stress factors is a sudden increase in the external pH. As a first step to understand the response of Bacillus subtil...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Wiegert T,Homuth G,Versteeg S,Schumann W

    更新日期:2001-07-01 00:00:00

  • Disruption of the GABA shunt affects mitochondrial respiration and virulence in the cereal pathogen Fusarium graminearum.

    abstract::The cereal pathogen Fusarium graminearum threatens food and feed production worldwide. It reduces the yield and poisons the remaining kernels with mycotoxins, notably deoxynivalenol (DON). We analyzed the importance of gamma-aminobutanoic acid (GABA) metabolism for the life cycle of this fungal pathogen. GABA metaboli...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Bönnighausen J,Gebhard D,Kröger C,Hadeler B,Tumforde T,Lieberei R,Bergemann J,Schäfer W,Bormann J

    更新日期:2015-12-01 00:00:00

  • Three conserved consensus sequences identify the NAD-binding site of ADP-ribosylating enzymes, expressed by eukaryotes, bacteria and T-even bacteriophages.

    abstract::It has been previously reported that the three-dimensional structures of the NAD-binding and catalytic site of bacterial toxins with ADP-ribosylating activity are superimposable, and that the key amino acids for the enzymatic activity are conserved. The model includes an NAD-binding and catalytic site formed by an alp...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


    authors: Domenighini M,Rappuoli R

    更新日期:1996-08-01 00:00:00

  • Iron-sulfur protein maturation in Helicobacter pylori: identifying a Nfu-type cluster carrier protein and its iron-sulfur protein targets.

    abstract::Helicobacter pylori is anomalous among non nitrogen-fixing bacteria in containing an incomplete NIF system for Fe-S cluster assembly comprising two essential proteins, NifS (cysteine desulfurase) and NifU (scaffold protein). Although nifU deletion strains cannot be obtained via the conventional gene replacement, a Nif...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Benoit SL,Holland AA,Johnson MK,Maier RJ

    更新日期:2018-05-01 00:00:00

  • Functional stratification of the Spitzenkörper of Neurospora crassa.

    abstract::GS-1 (ncu04189) is a protein required for the synthesis of beta-1,3-glucan in Neurospora crassa. As chitin, beta-1,3-glucan is a morphogenetically relevant component of the fungal cell wall. Previously, we showed that chitin synthases are delivered to the growing hyphal tip of N. crassa by secretory microvesicles that...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Verdín J,Bartnicki-Garcia S,Riquelme M

    更新日期:2009-12-01 00:00:00

  • A suf operon requirement for Fe-S cluster assembly during iron starvation in Escherichia coli.

    abstract::The suf and isc operons of Escherichia coli have been implicated in Fe-S cluster assembly. However, it has been unclear why E. coli has two systems for Fe-S cluster biosynthesis. We have examined the regulatory characteristics and mutant phenotypes of both operons to discern if the two operons have redundant functions...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Outten FW,Djaman O,Storz G

    更新日期:2004-05-01 00:00:00

  • Histone deacetylases in Trypanosoma brucei: two are essential and another is required for normal cell cycle progression.

    abstract::Reversible protein acetylation is established as a modification of major regulatory significance. In particular, histone acetylation regulates access to genetic information in eukaryotes. For example, class I and class II histone deacetylases are regulatory components of corepressor complexes involved in cell cycle pr...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Ingram AK,Horn D

    更新日期:2002-07-01 00:00:00

  • The non-ribosomal assembly and frequent occurrence of the protease inhibitors spumigins in the bloom-forming cyanobacterium Nodularia spumigena.

    abstract::Nodularia spumigena is a filamentous nitrogen-fixing cyanobacterium that forms toxic blooms in brackish water bodies worldwide. Spumigins are serine protease inhibitors reported from a single strain of N. spumigena isolated from the Baltic Sea. These linear tetrapeptides contain non-proteinogenic amino acids including...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Fewer DP,Jokela J,Rouhiainen L,Wahlsten M,Koskenniemi K,Stal LJ,Sivonen K

    更新日期:2009-09-01 00:00:00

  • Lytic transglycosylases RlpA and MltC assist in Vibrio cholerae daughter cell separation.

    abstract::The cell wall is a crucial structural feature in the vast majority of bacteria and comprises a covalently closed network of peptidoglycan (PG) strands. While PG synthesis is important for survival under many conditions, the cell wall is also a dynamic structure, undergoing degradation and remodeling by 'autolysins', e...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Weaver AI,Jiménez-Ruiz V,Tallavajhala SR,Ransegnola BP,Wong KQ,Dörr T

    更新日期:2019-10-01 00:00:00

  • The involvement of NAD(P)H dehydrogenase subunits, NdhD3 and NdhF3, in high-affinity CO2 uptake in Synechococcus sp. PCC7002 gives evidence for multiple NDH-1 complexes with specific roles in cyanobacteria.

    abstract::Random gene tagging was used to obtain new mutants of the marine cyanobacterium, Synechococcus sp. PCC7002, with defects in the CO2-concentrating mechanism (CCM). Two of these mutants, K22 and A41, showed poor growth at limiting CO2. Isolation and sequencing of a 6. 6 kb genomic region revealed the existence of five p...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Klughammer B,Sültemeyer D,Badger MR,Price GD

    更新日期:1999-06-01 00:00:00

  • Different residues in periplasmic domains of the CcmC inner membrane protein of Pseudomonas fluorescens ATCC 17400 are critical for cytochrome c biogenesis and pyoverdine-mediated iron uptake.

    abstract::The inner membrane protein CcmC (CytA) of Pseudomonas fluorescens ATCC17400, which has homologues in several bacteria and plant mitochondria, is needed for the biogenesis of cytochrome c. A CcmC-deficient mutant is also compromised in the production and utilization of pyoverdine, the high-affinity fluorescent sideroph...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Gaballa A,Baysse C,Koedam N,Muyldermans S,Cornelis P

    更新日期:1998-11-01 00:00:00

  • IS231D, E and F, three new insertion sequences in Bacillus thuringiensis: extension of the IS231 family.

    abstract::IS231 constitutes a family of insertion sequences widespread among Bacillus thuringiensis subspecies. Three new IS231 variants have been isolated from B. thuringiensis subspecies finitimus (IS231 D and E) and israelensis (IS231F). Like the previously described IS231A, B and C, these 1.7 kb elements display single open...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Rezsöhazy R,Hallet B,Delcour J

    更新日期:1992-07-01 00:00:00

  • RloC: a wobble nucleotide-excising and zinc-responsive bacterial tRNase.

    abstract:SUMMARY:The conserved bacterial protein RloC, a distant homologue of the tRNA(Lys) anticodon nuclease (ACNase) PrrC, is shown here to act as a wobble nucleotide-excising and Zn(++)-responsive tRNase. The more familiar PrrC is silenced by a genetically linked type I DNA restriction-modification (R-M) enzyme, activated b...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Davidov E,Kaufmann G

    更新日期:2008-09-01 00:00:00

  • In vitro reconstitution of a trimeric complex of DivIB, DivIC and FtsL, and their transient co-localization at the division site in Streptococcus pneumoniae.

    abstract::DivIB, DivIC and FtsL are bacterial proteins essential for cell division, which show interdependencies for their stabilities and localization. We have reconstituted in vitro a trimeric complex consisting of the recombinant extracellular domains of the three proteins from Streptococcus pneumoniae. The extracellular dom...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Noirclerc-Savoye M,Le Gouëllec A,Morlot C,Dideberg O,Vernet T,Zapun A

    更新日期:2005-01-01 00:00:00

  • Multiple hok genes on the chromosome of Escherichia coli.

    abstract::The hok/sok locus of plasmid R1 mediates plasmid stabilization by the killing of plasmid-free cells. Many bacterial plasmids carry similar loci. For example, the F plasmid carries two hok homologues, flm and srnB, that mediate plasmid stabilization by this specialized type of programmed cell death. Here, we show that ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Pedersen K,Gerdes K

    更新日期:1999-06-01 00:00:00

  • A member of the cAMP receptor protein family of transcription regulators in Mycobacterium tuberculosis is required for virulence in mice and controls transcription of the rpfA gene coding for a resuscitation promoting factor.

    abstract::Deletion of gene Rv3676 in Mycobacterium tuberculosis coding for a transcription factor belonging to the cAMP receptor protein (CRP) family caused growth defects in laboratory medium, in bone marrow-derived macrophages and in a mouse model of tuberculosis. Transcript profiling of M. tuberculosis grown in vitro identif...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Rickman L,Scott C,Hunt DM,Hutchinson T,Menéndez MC,Whalan R,Hinds J,Colston MJ,Green J,Buxton RS

    更新日期:2005-06-01 00:00:00

  • Translation elongation factor 3: a fungus-specific translation factor?

    abstract::Fungi appear to be unique in their requirement for a third soluble translation elongation factor. This factor, designated elongation factor 3 (EF-3), was first described in the yeast Saccharomyces cerevisiae and has subsequently been identified in a wide range of fungal species including Candida albicans and Schizosac...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


    authors: Belfield GP,Tuite MF

    更新日期:1993-08-01 00:00:00

  • Involvement of fixLJ in the regulation of nitrogen fixation in Azorhizobium caulinodans.

    abstract::A gene bank of Azorhizobium caulinodans DNA constructed in the bacteriophage lambda GEM11 was screened with Rhizobium meliloti fixL and fixJ genes as probes. One positive recombinant phage, ORS lambda L, was isolated. The nucleotide sequence of a 3.7 kb fragment was established. Two open reading frames of 1512bp and 6...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Kaminski PA,Elmerich C

    更新日期:1991-03-01 00:00:00

  • Identification and characterization of genes encoding sex pheromone cAM373 activity in Enterococcus faecalis and Staphylococcus aureus.

    abstract::The sex pheromone cAM373 of Enterococcus faecalis and the related staph-cAM373 of Staphylococcus aureus were found to correspond to heptapeptides located within the C-termini of the signal sequences of putative prelipoproteins. The deduced mature forms of the lipoproteins share no detectable homology and presumably se...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Flannagan SE,Clewell DB

    更新日期:2002-05-01 00:00:00

  • The rpsL gene and streptomycin resistance in single and multiple drug-resistant strains of Mycobacterium tuberculosis.

    abstract::The recent emergence of indolent and rapidly fatal drug-resistant strains of Mycobacterium tuberculosis has renewed interest in defining the molecular mechanisms of drug resistance in the tubercle bacilli. In this report, we have examined the mechanism of resistance to streptomycin (Sm) in M. tuberculosis through the ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Nair J,Rouse DA,Bai GH,Morris SL

    更新日期:1993-11-01 00:00:00

  • Rga5p is a specific Rho1p GTPase-activating protein that regulates cell integrity in Schizosaccharomyces pombe.

    abstract::Schizosaccharomyces pombe Rho1p regulates (1,3)beta-d-glucan synthesis and is required for cell integrity maintenance and actin cytoskeleton organization, but nothing is known about the regulation of this protein. At least nine different S. pombe genes code for proteins predicted to act as Rho GTPase-activating protei...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Calonge TM,Arellano M,Coll PM,Perez P

    更新日期:2003-01-01 00:00:00

  • The essential cell division protein FtsN contains a critical disulfide bond in a non-essential domain.

    abstract::Disulfide bonds are found in many proteins associated with the cell wall of Escherichia coli, and for some of these proteins the disulfide bond is critical to their stability and function. One protein found to contain a disulfide bond is the essential cell division protein FtsN, but the importance of this bond to the ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Meehan BM,Landeta C,Boyd D,Beckwith J

    更新日期:2017-02-01 00:00:00

  • A holin/peptidoglycan hydrolase-dependent protein secretion system.

    abstract::Gram-negative bacteria have evolved numerous pathways to secrete proteins across their complex cell envelopes. Here, we describe a protein secretion system that uses a holin membrane protein in tandem with a cell wall-editing enzyme to mediate the secretion of substrate proteins from the periplasm to the cell exterior...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Palmer T,Finney AJ,Saha CK,Atkinson GC,Sargent F

    更新日期:2020-09-04 00:00:00

  • yst gene expression in Yersinia enterocolitica is positively regulated by a chromosomal region that is highly homologous to Escherichia coli host factor 1 gene (hfq).

    abstract::Yersinia enterocolitica produces heat-stable enterotoxin (Y-ST) as one of its virulence factors. The yst gene, however, frequently and spontaneously becomes inactive (silent) during storage, which is accompanied by concurrent changes in some biological properties such as colony morphology, growth rate, carbon fermenta...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Nakao H,Watanabe H,Nakayama S,Takeda T

    更新日期:1995-12-01 00:00:00

  • Four signalling domains in the hybrid histidine protein kinase RodK of Myxococcus xanthus are required for activity.

    abstract::In prokaryotes, the principal signal transduction systems operating at the level of protein phosphorylation are the two-component systems. A number of hybrid histidine protein kinases in these systems contain several receiver domains, however, the function of these receiver domains is unknown. The RodK kinase in Myxoc...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Rasmussen AA,Wegener-Feldbrügge S,Porter SL,Armitage JP,Søgaard-Andersen L

    更新日期:2006-04-01 00:00:00

  • Quorum regulatory small RNAs repress type VI secretion in Vibrio cholerae.

    abstract::Type VI secretion is critical for Vibrio cholerae to successfully combat phagocytic eukaryotes and to survive in the presence of competing bacterial species. V. cholerae type VI secretion system genes are encoded in one large and two small clusters. In V. cholerae, type VI secretion is controlled by quorum sensing, th...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Shao Y,Bassler BL

    更新日期:2014-06-01 00:00:00

  • Organization of the genes necessary for hydrogenase expression in Rhodobacter capsulatus. Sequence analysis and identification of two hyp regulatory mutants.

    abstract::A 25 kbp DNA fragment from the chromosome of Rhodobacter capsulatus B10 carrying hydrogenase (hup) determinants was completely sequenced. Coding regions corresponding to 20 open reading frames were identified. The R. capsulatus hydrogenase-specific gene (hup and hyp) products bear significant structural identity to hy...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Colbeau A,Richaud P,Toussaint B,Caballero FJ,Elster C,Delphin C,Smith RL,Chabert J,Vignais PM

    更新日期:1993-04-01 00:00:00

  • Promoter selectivity control of Escherichia coli RNA polymerase by ionic strength: differential recognition of osmoregulated promoters by E sigma D and E sigma S holoenzymes.

    abstract::Transcription in vitro of two osmoregulated promoters, for the Escherichia coli osmB and osmY genes, was analysed using two species of RNA polymerase holoenzyme reconstituted from purified core enzyme and either sigma D (sigma 70, the major sigma in exponentially growing cells) or sigma S (sigma 38, the principal sigm...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Ding Q,Kusano S,Villarejo M,Ishihama A

    更新日期:1995-05-01 00:00:00

  • Search for poly(A) polymerase targets in E. coli reveals its implication in surveillance of Glu tRNA processing and degradation of stable RNAs.

    abstract::Polyadenylation is a universal post-transcriptional modification involved in degradation and quality control of bacterial RNAs. In Escherichia coli, it is admitted that any accessible RNA 3' end can be tagged by a poly(A) tail for decay. However, we do not have yet an overall view of the population of polyadenylated m...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Maes A,Gracia C,Hajnsdorf E,Régnier P

    更新日期:2012-01-01 00:00:00