Resistance mutations reveal the atovaquone-binding domain of cytochrome b in malaria parasites.

abstract：:Expression of the 987P gene cluster is activated by the adjacent IS1 element of an STpa transposon. Nucleotide sequence analysis of the 987P-DNA region contiguous with this IS1 element revealed the presence of an open reading frame designated fapR, encoding a basic protein of 260 amino acid residues with a molecular m...

journal_title：Molecular microbiology

authors： Klaasen P,de Graaf FK

更新日期：1990-10-01 00:00:00

abstract：:Iron-sulfur (Fe-S) clusters are prosthetic groups on proteins that function in a range of enzymatic and electron transfer reactions. Fe-S cluster synthesis is essential for the survival of all eukaryotes. Independent Fe-S cluster biosynthesis pathways occur in the mitochondrion, plastid, and cytosolic compartments of ...

journal_title：Molecular microbiology

authors： Aw YTV,Seidi A,Hayward JA,Lee J,Makota FV,Rug M,van Dooren GG

更新日期：2020-11-22 00:00:00

abstract：:We report the cloning and determination of the nucleotide sequence of the gene encoding nucleoside diphosphate kinase (Ndk) from Pseudomonas aeruginosa. The amino acid sequence of Ndk was highly homologous with other known bacterial and eukaryotic Ndks (39.9 to 58.3% amino acid identity). We have previously reported t...

journal_title：Molecular microbiology

authors： Sundin GW,Shankar S,Chugani SA,Chopade BA,Kavanaugh-Black A,Chakrabarty AM

更新日期：1996-06-01 00:00:00

abstract：:We have cloned, characterized and inactivated genes encoding putative UspA paralogues in Escherichia coli. The yecG (uspC), yiiT (uspD) and ydaA (uspE) genes were demonstrated to encode protein pro-ducts and these were mapped to spots in the E. coli proteomic database. Expression analysis using chromosomal transcripti...

journal_title：Molecular microbiology

authors： Gustavsson N,Diez A,Nyström T

更新日期：2002-01-01 00:00:00

abstract：:Successful pathogens must be able to swiftly respond to and repair DNA damages inflicted by the host defence. The replication protein A (RPA) complex plays multiple roles in DNA damage response and is regulated by phosphorylation. However, the regulators of RPA phosphorylation remain unclear. Here, we investigated Rfa...

journal_title：Molecular microbiology

authors： Gao J,Wang H,Wong AH,Zeng G,Huang Z,Wang Y,Sang J,Wang Y

更新日期：2014-10-01 00:00:00

abstract：:It has been previously reported that the three-dimensional structures of the NAD-binding and catalytic site of bacterial toxins with ADP-ribosylating activity are superimposable, and that the key amino acids for the enzymatic activity are conserved. The model includes an NAD-binding and catalytic site formed by an alp...

journal_title：Molecular microbiology

authors： Domenighini M,Rappuoli R

更新日期：1996-08-01 00:00:00

abstract：:A complete understanding of host-parasite interactions must necessarily include the identification and characterization of gene products expressed by both parties during the infectious process. We have developed a new screen to identify bacterial genes that are transcriptionally induced during infection of a host anim...

journal_title：Molecular microbiology

authors： Camilli A,Mekalanos JJ

更新日期：1995-11-01 00:00:00

abstract：:The threonine dehydratase IlvA is part of the isoleucine biosynthesis pathway in the Gram-positive model bacterium Bacillus subtilis. Consequently, deletion of ilvA causes isoleucine auxotrophy. It has been reported that ilvA pseudo-revertants having a derepressed hom-thrCB operon appear in the presence of threonine. ...

journal_title：Molecular microbiology

authors： Rosenberg J,Müller P,Lentes S,Thiele MJ,Zeigler DR,Tödter D,Paulus H,Brantl S,Stülke J,Commichau FM

更新日期：2016-09-01 00:00:00

abstract：:The rotA gene of Escherichia coli encodes a peptidyl-prolyl cis/trans isomerase (PPIase), which is supposed to catalyse protein folding in the periplasm. To investigate the importance of the enzyme, the rotA gene was cloned and a chromosomal deletion mutant was created. The rotA mutant was normally viable. No residual...

journal_title：Molecular microbiology

authors： Kleerebezem M,Heutink M,Tommassen J

更新日期：1995-10-01 00:00:00

abstract：:The genome of uropathogenic Escherichia coli isolate 536 contains five well-characterized pathogenicity islands (PAIs) encoding key virulence factors of this strain. Except PAI IV(536), the four other PAIs of strain 536 are flanked by direct repeats (DRs), carry intact integrase genes and are able to excise site-speci...

journal_title：Molecular microbiology

authors： Hochhut B,Wilde C,Balling G,Middendorf B,Dobrindt U,Brzuszkiewicz E,Gottschalk G,Carniel E,Hacker J

更新日期：2006-08-01 00:00:00

abstract：:The creA gene of A. nidulans encodes a wide-domain regulatory protein mediating carbon catabolite repression. Northern blot analysis of creA mRNA revealed a complex expression profile: the addition of monosaccharides to a carbon-starved culture of A. nidulans provoked a strong transient stimulation of creA transcript ...

journal_title：Molecular microbiology

authors： Strauss J,Horvath HK,Abdallah BM,Kindermann J,Mach RL,Kubicek CP

更新日期：1999-04-01 00:00:00

abstract：:Phytopathogenic bacteria possess a large number of genes that allow them to grow and cause disease on plants. Many of these genes should be induced when the bacteria come in contact with plant tissue. We used a modified in vivo expression technology (IVET) approach to identify genes from the plant pathogen Pseudomonas...

journal_title：Molecular microbiology

authors： Boch J,Joardar V,Gao L,Robertson TL,Lim M,Kunkel BN

更新日期：2002-04-01 00:00:00

abstract：:The differences between archaeal and bacterial flagella are becoming more apparent as research on the archaeal structure progresses. One crucial difference is the presence of a leader peptide on archaeal preflagellins, which is removed from the flagellin prior to its incorporation into the flagellar filament. The enzy...

journal_title：Molecular microbiology

authors： Bardy SL,Jarrell KF

更新日期：2003-11-01 00:00:00

abstract：:External factors regulate the formation of pigment protein complexes in facultatively photosynthetic bacteria. The puf operon of Rhodobacter capsulatus encodes the pigment binding proteins of the reaction centre and light-harvesting I complex. Here we demonstrate that a single base-pair exchange within a sequence of d...

journal_title：Molecular microbiology

authors： Klug G,Gad'on N,Jock S,Narro ML

更新日期：1991-05-01 00:00:00

abstract：:Many fundamental cellular processes depend on enzymes that utilize chemical energy to catalyse unfavourable reactions. Certain classes of ATPases provide a particularly vivid example of the process of energy conversion, employing cycles of nucleotide turnover to move and/or rearrange biological polymers such as protei...

journal_title：Molecular microbiology

authors： Thomsen ND,Berger JM

更新日期：2008-09-01 00:00:00

abstract：:The interactions between pathogenic bacteria and the host need to be resolved at the molecular level in order to develop novel vaccines and drugs. We have previously identified strepadhesin, a novel glycoprotein-binding activity in Streptococcus pyogenes, which is regulated by Mga, a regulator of streptococcal virulen...

journal_title：Molecular microbiology

authors： Hytönen J,Haataja S,Gerlach D,Podbielski A,Finne J

更新日期：2001-01-01 00:00:00

abstract：:Pneumococcus has been shown to bind to epithelial cells of the nasopharynx and lung, and to endothelial cells of the peripheral vasculature. To characterize bacterial elements required for attachment to these cell types, a library of genetically altered pneumococci with defects in exported proteins was screened for th...

journal_title：Molecular microbiology

authors： Spellerberg B,Cundell DR,Sandros J,Pearce BJ,Idanpaan-Heikkila I,Rosenow C,Masure HR

更新日期：1996-02-01 00:00:00

abstract：:Spatio-temporal oscillations of the Min proteins are essential for selecting the cell division site in Escherichia coli. These oscillations are a key example of a biological phenomenon that can only be understood on a systems level rather than on the level of its individual components. Here, we review the key concepts...

journal_title：Molecular microbiology

authors： Kruse K,Howard M,Margolin W

更新日期：2007-03-01 00:00:00

abstract：:We report selective phosphorylation of the DNA-binding domain of the Streptococcus pneumoniae transcriptional regulator RitR. RitR is annotated as a two-component response regulator, but lacks a cognate His kinase as a neighbouring locus in the genome. In addition, Asn replaces Asp at the expected acceptor site. By th...

journal_title：Molecular microbiology

authors： Ulijasz AT,Falk SP,Weisblum B

更新日期：2009-01-01 00:00:00

abstract：:Rifampicin resistance, a defining attribute of multidrug-resistant tuberculosis, is conferred by mutations in the β subunit of RNA polymerase. Sequencing of rifampicin-resistant (RIF-R) clinical isolates of Mycobacterium tuberculosis revealed, in addition to RIF-R mutations, enrichment of potential compensatory mutati...

journal_title：Molecular microbiology

authors： Song T,Park Y,Shamputa IC,Seo S,Lee SY,Jeon HS,Choi H,Lee M,Glynne RJ,Barnes SW,Walker JR,Batalov S,Yusim K,Feng S,Tung CS,Theiler J,Via LE,Boshoff HI,Murakami KS,Korber B,Barry CE 3rd,Cho SN

更新日期：2014-03-01 00:00:00

abstract：:A collection of 20 strains of Helicobacter pylori from several regions of the world was studied to better understand the population genetic structure and diversity of this species. Sequences of fragments from seven housekeeping genes (atpA, efp, mutY, ppa, trpC, ureI, yphC ) and two virulence-associated genes (cagA, v...

journal_title：Molecular microbiology

authors： Achtman M,Azuma T,Berg DE,Ito Y,Morelli G,Pan ZJ,Suerbaum S,Thompson SA,van der Ende A,van Doorn LJ

更新日期：1999-05-01 00:00:00

abstract：:The conserved cAMP-dependent protein kinase (PKA) plays critical roles in the regulation of morphological transitions and virulence in the human fungal pathogen Candida albicans. It has long been thought that the PKA catalytic subunit is essential for cell viability in this fungus. Paradoxically, the single adenylyl c...

journal_title：Molecular microbiology

authors： Cao C,Wu M,Bing J,Tao L,Ding X,Liu X,Huang G

更新日期：2017-07-01 00:00:00

abstract：:Gram-negative bacteria have evolved numerous pathways to secrete proteins across their complex cell envelopes. Here, we describe a protein secretion system that uses a holin membrane protein in tandem with a cell wall-editing enzyme to mediate the secretion of substrate proteins from the periplasm to the cell exterior...

journal_title：Molecular microbiology

authors： Palmer T,Finney AJ,Saha CK,Atkinson GC,Sargent F

更新日期：2020-09-04 00:00:00

abstract：:Cell division in prokaryotes initiates with assembly of the Z-ring at midcell, which, in Escherichia coli, is tethered to the inner leaflet of the cytoplasmic membrane through a direct interaction with FtsA, a widely conserved actin homolog. The Z-ring is comprised of polymers of tubulin-like FtsZ and has been suggest...

journal_title：Molecular microbiology

authors： Conti J,Viola MG,Camberg JL

更新日期：2018-02-01 00:00:00

abstract：:In bacteria, genes are often expressed from multiple promoters to allow for a greater spectrum of regulation. Transcription of rRNA genes in Escherichia coli uses two promoters, rrn P1 and rrn P2. Under the conditions examined previously, the P1 and P2 promoters were regulated in response to many of the same changes i...

journal_title：Molecular microbiology

authors： Murray HD,Gourse RL

更新日期：2004-06-01 00:00:00

abstract：:Protein quality control and proteolysis are involved in cell maintenance and environmental acclimatization in bacteria and eukaryotes. The AAA protease FtsH2 of the cyanobacterium Synechocystis sp. PCC 6803 was identified during a screening for mutants impaired in osmoregulation. The ftsH2(-) mutant was salt sensitive...

journal_title：Molecular microbiology

authors： Stirnberg M,Fulda S,Huckauf J,Hagemann M,Krämer R,Marin K

更新日期：2007-01-01 00:00:00

abstract：:Sinorhizobium meliloti and host legumes enter into a nitrogen-fixing, symbiotic relationship triggered by an exchange of signals between bacteria and plant. S. meliloti produces Nod factor, which elicits the formation of nodules on plant roots, and succinoglycan, an exopolysaccharide that allows for bacterial invasion...

journal_title：Molecular microbiology

authors： Wells DH,Long SR

更新日期：2002-03-01 00:00:00

abstract：:The export of large negatively charged capsular polysaccharides across the outer membrane represents a significant challenge to Gram negative bacteria. In the case of Escherichia coli group 2 capsular polysaccharides, the mechanism of export across the outer membrane was unknown, with no identified candidate outer mem...

journal_title：Molecular microbiology

authors： McNulty C,Thompson J,Barrett B,Lord L,Andersen C,Roberts IS

更新日期：2006-02-01 00:00:00

abstract：:The mitis group streptococci (MGS) are widespread in the oral cavity and are traditionally associated with oral health. However, these organisms have many attributes that contribute to the development of pathogenic oral communities. MGS adhere rapidly to saliva-coated tooth surfaces, thereby providing an attachment su...

journal_title：Molecular microbiology

authors： Whitmore SE,Lamont RJ

更新日期：2011-07-01 00:00:00

abstract：:Structural maintenance of chromosome (SMC) and the SMC-interacting kleisin protein families have key functions in the chromosome organization of most organisms. Here, we report that the Bacillus subtilis kleisin, ScpA, can form a ternary complex with the SMC and ScpB proteins in a yeast tri-hybrid assay, supporting th...

journal_title：Molecular microbiology

authors： Dervyn E,Noirot-Gros MF,Mervelet P,McGovern S,Ehrlich SD,Polard P,Noirot P

更新日期：2004-03-01 00:00:00