Search for poly(A) polymerase targets in E. coli reveals its implication in surveillance of Glu tRNA processing and degradation of stable RNAs.

Abstract:

:Polyadenylation is a universal post-transcriptional modification involved in degradation and quality control of bacterial RNAs. In Escherichia coli, it is admitted that any accessible RNA 3' end can be tagged by a poly(A) tail for decay. However, we do not have yet an overall view of the population of polyadenylated molecules. The sampling of polyadenylated RNAs presented here demonstrates that rRNA fragments and tRNA precursors originating from the internal spacer regions of the rrn operons, in particular, rrnB are abundant poly(A) polymerase targets. Focused analysis showed that Glu tRNA precursors originating from the rrnB and rrnG transcripts exhibit long 3' trailers that are primarily removed by PNPase and to a lesser extent by RNase II and poly(A) polymerase. Moreover, 3' trimming by exoribonucleases precedes 5' end maturation by RNase P. Interestingly, characterization of RNA fragments that accumulate in a PNPase deficient strain showed that Glu tRNA precursors still harbouring the 5' leader can be degraded by a 3' to 5' quality control pathway involving poly(A) polymerase. This demonstrates that the surveillance of tRNA maturation described for a defective tRNA also applies to a wild-type tRNA.

journal_name

Mol Microbiol

journal_title

Molecular microbiology

authors

Maes A,Gracia C,Hajnsdorf E,Régnier P

doi

10.1111/j.1365-2958.2011.07943.x

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

436-51

issue

2

eissn

0950-382X

issn

1365-2958

journal_volume

83

pub_type

杂志文章
  • Towards an understanding of the structural basis of 'forbidden' transport pathways in the Escherichia coli lactose carrier: mutations probing the energy barriers to uncoupled transport.

    abstract::Recent progress in the analysis of mutants of the Escherichia coli lactose carrier function is reviewed, with special emphasis on the structural basis for energy barriers which prevent 'forbidden' conformational changes. Mutations which break down the barriers to forbidden isomerizations involving the binary carrier:s...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2958.1990.tb02053.x

    authors: King SC,Wilson TH

    更新日期:1990-09-01 00:00:00

  • Metabolic adaptation in Cryptococcus neoformans during early murine pulmonary infection.

    abstract:SUMMARY:The pathogenic fungus Cryptococcus neoformans generally initiates infection in mammalian lung tissue and subsequently disseminates to the brain. We performed serial analysis of gene expression (SAGE) on C. neoformans cells recovered from the lungs of mice and found elevated expression of genes for central carbo...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2008.06374.x

    authors: Hu G,Cheng PY,Sham A,Perfect JR,Kronstad JW

    更新日期:2008-09-01 00:00:00

  • Three-component-mediated serotype conversion in Pseudomonas aeruginosa by bacteriophage D3.

    abstract::Bacteriophage D3 is capable of lysogenizing Pseudomonas aeruginosa PAO1 (serotype O5), converting the O-antigen from O5 to O16 and O-acetylating the N-acetylfucosamine moiety. To investigate the mechanism of lysogenic conversion, a 3.6 kb fragment from the D3 genome was isolated capable of mediating serotypic conversi...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2001.02311.x

    authors: Newton GJ,Daniels C,Burrows LL,Kropinski AM,Clarke AJ,Lam JS

    更新日期:2001-03-01 00:00:00

  • The role of the NAC protein in the nitrogen regulation of Klebsiella aerogenes.

    abstract::The NAC (nitrogen assimilation control) protein from Klebsiella aerogenes is a LysR-like regulator for transcription of several operons involved in nitrogen metabolism, and couples the transcription of these sigma 70-dependent operons to regulation by the sigma 54-dependent NTR system. NAC activates expression of oper...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2958.1991.tb01965.x

    authors: Bender RA

    更新日期:1991-11-01 00:00:00

  • Phospholipases C are involved in the virulence of Mycobacterium tuberculosis.

    abstract::Phospholipases C play a role in the pathogenesis of several bacteria. Mycobacterium tuberculosis, the causative agent of tuberculosis, possesses four genes encoding putative phospholipases C, plcA, plcB, plcC and plcD. However, the contribution of these genes to virulence is unknown. We constructed four single mutants...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2002.03009.x

    authors: Raynaud C,Guilhot C,Rauzier J,Bordat Y,Pelicic V,Manganelli R,Smith I,Gicquel B,Jackson M

    更新日期:2002-07-01 00:00:00

  • The Rhodobacter capsulatus hupSLC promoter: identification of cis-regulatory elements and of trans-activating factors involved in H2 activation of hupSLC transcription.

    abstract::The [NiFe]hydrogenase of the photosynthetic bacterium Rhodobacter capsulatus is encoded by the structural hupSLC operon, the expression of which is induced by H2. H2 activation was no longer observable in chromosomal hupR mutants, an indication that HupR is implicated directly in the activation by H2 of hupS gene expr...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1997.6291996.x

    authors: Toussaint B,de Sury d'Aspremont R,Delic-Attree I,Berchet V,Elsen S,Colbeau A,Dischert W,Lazzaroni Y,Vignais PM

    更新日期:1997-12-01 00:00:00

  • The role of the yeast plasma membrane SPS nutrient sensor in the metabolic response to extracellular amino acids.

    abstract::In response to discrete environmental cues, Saccharomyces cerevisiae cells adjust patterns of gene expression and protein activity to optimize metabolism. Nutrient-sensing systems situated in the plasma membrane (PM) of yeast have only recently been discovered. Ssy1p is one of three identified components of the Ssy1p-...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2001.02627.x

    authors: Forsberg H,Gilstring CF,Zargari A,Martínez P,Ljungdahl PO

    更新日期:2001-10-01 00:00:00

  • Ureaplasma antigenic variation beyond MBA phase variation: DNA inversions generating chimeric structures and switching in expression of the MBA N-terminal paralogue UU172.

    abstract::Phase variation of the major ureaplasma surface membrane protein, the multiple-banded antigen (MBA), with its counterpart, the UU376 protein, was recently discussed as a result of DNA inversion occurring at specific inverted repeats. Two similar inverted repeats to the ones within the mba locus were found in the genom...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2010.07474.x

    authors: Zimmerman CU,Rosengarten R,Spergser J

    更新日期:2011-02-01 00:00:00

  • A new piperidinol derivative targeting mycolic acid transport in Mycobacterium abscessus.

    abstract::The natural resistance of Mycobacterium abscessus to most commonly available antibiotics seriously limits chemotherapeutic treatment options, which is particularly challenging for cystic fibrosis patients infected with this rapid-growing mycobacterium. New drugs with novel molecular targets are urgently needed against...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.13406

    authors: Dupont C,Viljoen A,Dubar F,Blaise M,Bernut A,Pawlik A,Bouchier C,Brosch R,Guérardel Y,Lelièvre J,Ballell L,Herrmann JL,Biot C,Kremer L

    更新日期:2016-08-01 00:00:00

  • A study of the DNA damage checkpoint in Candida albicans: uncoupling of the functions of Rad53 in DNA repair, cell cycle regulation and genotoxic stress-induced polarized growth.

    abstract::In response to genotoxic stress (GS), Candida albicans can undergo polarized growth and massive genome rearrangements including loss-of-heterozygosity (LOH) events. We evaluated the contribution of the CaRad53p and CaDun1p kinases of the DNA damage checkpoint (DDCP) in these processes. Characterization of C. albicans ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.12471

    authors: Loll-Krippleber R,d'Enfert C,Feri A,Diogo D,Perin A,Marcet-Houben M,Bougnoux ME,Legrand M

    更新日期:2014-02-01 00:00:00

  • Dissecting functional similarities of ribosome-associated chaperones from Saccharomyces cerevisiae and Escherichia coli.

    abstract::Ribosome-tethered chaperones that interact with nascent polypeptide chains have been identified in both prokaryotic and eukaryotic systems. However, these ribosome-associated chaperones share no sequence similarity: bacterial trigger factors (TF) form an independent protein family while the yeast machinery is Hsp70-ba...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2005.04690.x

    authors: Rauch T,Hundley HA,Pfund C,Wegrzyn RD,Walter W,Kramer G,Kim SY,Craig EA,Deuerling E

    更新日期:2005-07-01 00:00:00

  • Testing the '+2 rule' for lipoprotein sorting in the Escherichia coli cell envelope with a new genetic selection.

    abstract::We report a novel strategy for selecting mutations that mislocalize lipoproteins within the Escherichia coli cell envelope and describe the mutants obtained. A strain carrying a deletion of the chromosomal malE gene, coding for the periplasmic maltose-binding protein (MalE), cannot use maltose unless a wild-type copy ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1999.01647.x

    authors: Seydel A,Gounon P,Pugsley AP

    更新日期:1999-11-01 00:00:00

  • The transcription of bradyzoite genes in Toxoplasma gondii is controlled by autonomous promoter elements.

    abstract::Experimental evidence suggests that apicomplexan parasites possess bipartite promoters with basal and regulated cis-elements similar to other eukaryotes. Using a dual luciferase model adapted for recombinational cloning and use in Toxoplasma gondii, we show that genomic regions flanking 16 parasite genes, which encomp...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2008.06249.x

    authors: Behnke MS,Radke JB,Smith AT,Sullivan WJ Jr,White MW

    更新日期:2008-06-01 00:00:00

  • Characterization of a novel fibronectin-binding surface protein in group A streptococci.

    abstract::Streptococcus pyogenes interacts with host fibronectin via distinct surface components. One of these components is the Sfbl protein (streptococcal fibronectin-binding protein, now specified as class I), an adhesin that represents a protein family with characteristic features. Here we present the complete structure of ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1995.mmi_17010137.x

    authors: Kreikemeyer B,Talay SR,Chhatwal GS

    更新日期:1995-07-01 00:00:00

  • Identification and characterization of genes encoding sex pheromone cAM373 activity in Enterococcus faecalis and Staphylococcus aureus.

    abstract::The sex pheromone cAM373 of Enterococcus faecalis and the related staph-cAM373 of Staphylococcus aureus were found to correspond to heptapeptides located within the C-termini of the signal sequences of putative prelipoproteins. The deduced mature forms of the lipoproteins share no detectable homology and presumably se...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2002.02922.x

    authors: Flannagan SE,Clewell DB

    更新日期:2002-05-01 00:00:00

  • The role of the sporulation gene spoIIIE in the regulation of prespore-specific gene expression in Bacillus subtilis.

    abstract::The spoIIIG gene encodes a sigma factor that determines prespore-specific gene expression during sporulation in Bacillus subtilis. Correct spatial and temporal expression of the spoIIIG gene depends on a number of other sporulation (spo) genes, but only one of these genes, spoIIIE, has a specific effect on spoIIIG exp...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1989.tb00275.x

    authors: Foulger D,Errington J

    更新日期:1989-09-01 00:00:00

  • Identification of Klebsiella pneumoniae virulence determinants using an intranasal infection model.

    abstract::Klebsiella pneumoniae is a Gram-negative enterobacterium that has historically been, and currently remains, a significant cause of human disease. It is a frequent cause of urinary tract infections and pneumonia, and subsequent systemic infections can have mortality rates as high as 60%. Despite its clinical significan...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2005.04918.x

    authors: Lawlor MS,Hsu J,Rick PD,Miller VL

    更新日期:2005-11-01 00:00:00

  • Incorporation of a polypeptide segment into the beta-domain pore during the assembly of a bacterial autotransporter.

    abstract::Bacterial autotransporters consist of an N-terminal 'passenger domain' that is transported into the extracellular space by an unknown mechanism and a C-terminal 'beta-domain' that forms a beta-barrel in the outer membrane. Recent studies have revealed that fully assembled autotransporters have an unusual architecture ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2007.06048.x

    authors: Ieva R,Skillman KM,Bernstein HD

    更新日期:2008-01-01 00:00:00

  • The CitST two-component system regulates the expression of the Mg-citrate transporter in Bacillus subtilis.

    abstract::citS and citT genes encoding a new two-component system were identified in the 71 degrees region between the pel and citM loci on the Bacillus subtilis chromosome. citS- and citT-deficient strains were unable to grow on minimal plates including citrate as a sole carbon source. In addition, a strain deficient in citM, ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2000.02055.x

    authors: Yamamoto H,Murata M,Sekiguchi J

    更新日期:2000-08-01 00:00:00

  • The inflammation-associated Salmonella SopA is a HECT-like E3 ubiquitin ligase.

    abstract::Salmonella translocate a group of type III effectors into the host cells to induce entry, promote survival and cause intestinal inflammation. Although the biochemical and cellular mechanisms of how bacterial effectors function inside host cells remain largely unknown, studies have indicated that a likely strategy is t...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2006.05407.x

    authors: Zhang Y,Higashide WM,McCormick BA,Chen J,Zhou D

    更新日期:2006-11-01 00:00:00

  • Another way to divide: the case of anammox bacteria.

    abstract::Anammox bacteria exhibit remarkable and characteristic metabolic features that enable them to oxidize ammonium under anoxic conditions. Their ultrastructure is complex, with three compartments, comprising paryphoplasm, the riboplasm and the anammoxosome. The latter is surrounded by ladderane lipids, creating a lipid-s...

    journal_title:Molecular microbiology

    pub_type: 评论,杂志文章

    doi:10.1111/j.1365-2958.2009.06839.x

    authors: Rachel R

    更新日期:2009-09-01 00:00:00

  • The transcriptional organization of the Bacillus subtilis 168 chromosome region between the spoVAF and serA genetic loci.

    abstract::The genetic organization of the spoVAF-serA area of the Bacillus subtilis chromosome and its putative transcription map have been derived from analysis of the nucleotide sequence. In order to confirm this transcription map as regards size of transcripts and to determine growth conditions for their appearance, we under...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1993.tb02671.x

    authors: Azevedo V,Sorokin A,Ehrlich SD,Serror P

    更新日期:1993-10-01 00:00:00

  • Two essential FtsH proteases control the level of the Fur repressor during iron deficiency in the cyanobacterium Synechocystis sp. PCC 6803.

    abstract::The cyanobacterium Synechocystis sp. PCC 6803 expresses four different FtsH protease subunits (FtsH1-4) that assemble into specific homo- and heterocomplexes. The FtsH2/FtsH3 complex is involved in photoprotection but the physiological roles of the other complexes, notably the essential FtsH1/FtsH3 complex, remain unc...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.12782

    authors: Krynická V,Tichý M,Krafl J,Yu J,Kaňa R,Boehm M,Nixon PJ,Komenda J

    更新日期:2014-11-01 00:00:00

  • Cloning and characterization of a relA/spoT homologue from Bacillus subtilis.

    abstract::A PCR-amplified DNA fragment of the relA gene from genomic Bacillus subtilis DNA was used to isolate the entire relA/spoT homologue and two adjacent open reading frames (ORFs) from a lambda ZAP Express library. The relA gene, which encodes a protein of 734 amino acid residues (aa), is flanked by an ORF (170aa) that sh...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1997.5511919.x

    authors: Wendrich TM,Marahiel MA

    更新日期:1997-10-01 00:00:00

  • Phenotypic repertoire of the FNR regulatory network in Escherichia coli.

    abstract::The FNR protein in Escherichia coli is an O(2) sensor that modifies global gene expression to adapt the cell to anaerobic growth. Regulation of FNR involves continuous cycling of the protein between its active and inactive states under aerobic conditions without apparent function. This raises the question of what bene...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2010.07437.x

    authors: Tolla DA,Savageau MA

    更新日期:2011-01-01 00:00:00

  • The role of a stress-response protein in Salmonella typhimurium virulence.

    abstract::We recently described the use of selective transposon mutagenesis to generate a series of avirulent mutants of a pathogenic strain of Salmonella typhimurium. Cloning and sequencing of the insertion sites from two of these mutants reveals that both have identical locations within an open reading frame that is highly ho...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1991.tb02122.x

    authors: Johnson K,Charles I,Dougan G,Pickard D,O'Gaora P,Costa G,Ali T,Miller I,Hormaeche C

    更新日期:1991-02-01 00:00:00

  • The pAM beta 1 CopF repressor regulates plasmid copy number by controlling transcription of the repE gene.

    abstract::pAM beta 1 is a low-copy-number, promiscuous plasmid from Gram-positive bacteria that replicates by a unidirectional theta-type mode. Its replication is initiated by an original mechanism, involving the positive rate-limiting RepE protein. Here we show that the pAM beta 1-encoded CopF protein is involved in negative r...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1994.tb02181.x

    authors: Le Chatelier E,Ehrlich SD,Jannière L

    更新日期:1994-11-01 00:00:00

  • Differential transcriptome profiling identifies Plasmodium genes encoding pre-erythrocytic stage-specific proteins.

    abstract::Invasive sporozoite and merozoite stages of malaria parasites that infect mammals enter and subsequently reside in hepatocytes and red blood cells respectively. Each invasive stage may exhibit unique adaptations that allow it to interact with and survive in its distinct host cell environment, and these adaptations are...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2003.03909.x

    authors: Kaiser K,Matuschewski K,Camargo N,Ross J,Kappe SH

    更新日期:2004-03-01 00:00:00

  • Characterization of Helicobacter pylori HP0231 (DsbK): role in disulfide bond formation, redox homeostasis and production of Helicobacter cystein-rich protein HcpE.

    abstract::Helicobacter pylori is a human gastric pathogen that colonizes ∼ 50% of the world's population. It can cause gastritis, gastric or duodenal ulcers and also gastric cancer. The numerous side effects of available treatments and the emergence of antibiotic resistant strains are severe concerns that justify further resear...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.12923

    authors: Lester J,Kichler S,Oickle B,Fairweather S,Oberc A,Chahal J,Ratnayake D,Creuzenet C

    更新日期:2015-04-01 00:00:00

  • Genetic characterization of a Rhizobium meliloti lactose utilization locus.

    abstract::We identified several linked genes of a lactose regulon in Rhizobium meliloti. These were lacZ, the structural gene for beta-galactosidase; lacR, the lactose repressor gene; and two genes encoding proteins of unknown function, lacW and lacX. Insertion mutants in lacW and lacZ belonged to a single genetic complementati...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1994.tb00298.x

    authors: Jelesko JG,Leigh JA

    更新日期:1994-01-01 00:00:00