Abstract:
:Messenger RNA decay in Bacillus subtilis is accomplished by a combination of exoribonucleases and endoribonucleases. Intermediates in the decay process have not been readily detectable, and previous studies on mRNA decay have used a handful of highly expressed transcripts as models. Here, we use RNA-Seq analysis to probe mRNA turnover globally. A significant fraction of messages showed differential accumulation of RNA fragments that mapped near the 5' or 3' end of the coding sequence, consistent with initiation of decay from either the 5' end or from an internal cleavage site. Patterns of mRNA decay in the wild type were compared with patterns in a mutant strain lacking polynucleotide phosphorylase (PNPase), which is considered the major 3' exonuclease activity in mRNA decay and which is one of four known 3' exonucleases in B. subtilis. The results showed a striking dependence on PNPase for mRNA turnover in many cases, suggesting specificity in the ability of 3' exonucleases to degrade from 3'-hydroxyl termini. RNA-Seq data demonstrated a sharp decrease in expression of Sigma D in the PNPase-deletion strain. Reduction in sigD regulon expression explained the chain growth phenotype of the PNPase mutant and also predicted a defect in swarming motility.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Liu B,Deikus G,Bree A,Durand S,Kearns DB,Bechhofer DHdoi
10.1111/mmi.12748subject
Has Abstractpub_date
2014-10-01 00:00:00pages
41-55issue
1eissn
0950-382Xissn
1365-2958journal_volume
94pub_type
杂志文章abstract::In enteric bacteria, proteins of the Hha/YmoA family play a role in the regulation of gene expression in response to environmental factors. Interaction of both Hha and YmoA with H-NS has been reported, and an Hha/H-NS complex has been shown to modulate expression in Escherichia coli of the haemolysin operon of plasmid...
journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2004-10-01 00:00:00
abstract::OmpR activates expression of the two-component regulatory system located on Salmonella pathogenicity island 2 (SPI-2) that controls the expression of a type III secretion system, as well as many other genes required for systemic infection in mice. Measurements of SsrA and SsrB protein levels under different growth con...
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pub_type: 杂志文章
doi:10.1111/j.1365-2958.2004.04317.x
更新日期:2004-11-01 00:00:00
abstract::GPI-anchoring is a universal and critical post-translational protein modification in eukaryotes. In fungi, many cell wall proteins are GPI-anchored, and disruption of GPI-anchored proteins impairs cell wall integrity. After being synthesized and attached to target proteins, GPI anchors undergo modification on lipid mo...
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journal_title:Molecular microbiology
pub_type: 杂志文章
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1993.tb01689.x
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abstract::A respiratory quinol oxidase complex that is encoded by the soxABCD operon has been purified from the thermoacidophilic archaeon Sulfolobus acidocaldarius. The enzyme was solubilized with dodecyl maltoside and purified in the presence of this detergent and ethylene glycol. The complex is hydrodynamically homogeneous a...
journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1994-07-01 00:00:00
abstract::The Salmonella flagellar motor consists of a rotor and about a dozen stator elements. Each stator element, consisting of MotA and MotB, acts as a proton channel to couple proton flow with torque generation. A highly conserved Asp33 residue of MotB is directly involved in the energy coupling mechanism, but it remains u...
journal_title:Molecular microbiology
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更新日期:2014-01-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1991-03-01 00:00:00
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journal_title:Molecular microbiology
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2014-11-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2006-11-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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journal_title:Molecular microbiology
pub_type: 杂志文章
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1993-01-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1991.tb00799.x
更新日期:1991-06-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1998.01043.x
更新日期:1998-10-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章,评审
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journal_title:Molecular microbiology
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abstract::The ZmpC zinc metalloproteinase of Streptococcus pneumoniae, annotated in the type 4 genome as SP0071, was found to cleave human matrix metalloproteinase 9 (MMP-9). The previously described IgA protease activity was confirmed to be specifically linked to the IgA1-protease/SP1154 zinc metalloproteinase. MMP-9 is a prot...
journal_title:Molecular microbiology
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2017-06-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1994-02-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2007-06-01 00:00:00
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pub_type: 杂志文章
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2001-02-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2015-10-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2011-11-01 00:00:00