Abstract:
:The efficiency of conjugation of F-like plasmids is regulated by the FinOP fertility inhibition system. The transfer (tra) operon is under the direct control of the TraJ transcriptional activator which, in turn, is negatively regulated by FinP, an antisense RNA, and FinO, a 22 kDa protein. Recently, FinO has been shown to extend the chemical stability of FinP in vivo in the absence of traJ mRNA. The in vitro secondary structures of both the FinP and TraJ RNAs were determined by the use of single- and double-strand-specific nucleases; both RNAs were found to have double stem-loop structures that are complementary to each other and, therefore, FinP RNA and TraJ RNA have the potential to form a duplex with each other. This was verified by in vitro binding experiments. The reaction was shown to be biomolecular with an apparent rate constant (kapp) of 5 x 10(5)M-1s-1, a value that is similar to those found for other natural antisense RNA systems. Preliminary evidence for the in vivo formation of the FinP-TraJ RNA duplex was obtained by primer extension of the traJ mRNA; the presence of both FinO and FinP was required to cause a dramatic reduction in the steady-state level of traJ mRNA, perhaps as a result of RNase III degradation of the resulting RNA duplex.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
van Biesen T,Söderbom F,Wagner EG,Frost LSdoi
10.1111/j.1365-2958.1993.tb00901.xsubject
Has Abstractpub_date
1993-10-01 00:00:00pages
35-43issue
1eissn
0950-382Xissn
1365-2958journal_volume
10pub_type
杂志文章abstract::The SaPIs are chromosomal islands in staphylococci and other Gram-positive bacteria that carry genes for superantigens, virulence factors, resistance and certain metabolic functions. They have intimate relationships with certain temperate phages involving phage-induced excision, replication and efficient packaging in ...
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journal_title:Molecular microbiology
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pub_type: 杂志文章
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journal_title:Molecular microbiology
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2005-12-01 00:00:00
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更新日期:1993-01-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1111/j.1365-2958.1995.tb02290.x
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pub_type: 杂志文章
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更新日期:1991-11-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1999-01-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 评论,杂志文章
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1993.tb00935.x
更新日期:1993-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-11-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1996.tb02473.x
更新日期:1996-03-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.14236
更新日期:2019-06-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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