Cellular levels and activity of the flagellar sigma factor FliA of Escherichia coli are controlled by FlgM-modulated proteolysis.

abstract：:A family of nine Salmonella typhimurium type III secretion effectors with a conserved amino-terminus have been defined. Three family members (SifA, SifB and SseJ) have previously been demonstrated to localize to the Salmonella-containing vacuole and to Salmonella-induced filaments. In contrast, we demonstrate that two...

journal_title：Molecular microbiology

authors： Miao EA,Brittnacher M,Haraga A,Jeng RL,Welch MD,Miller SI

更新日期：2003-04-01 00:00:00

abstract：:The earliest event in bacterial cell division is the formation of a Z ring, composed of the tubulin-like FtsZ protein, at the division site at midcell. This ring then recruits several other division proteins and together they drive the formation of a division septum between two replicated chromosomes. Here we show tha...

journal_title：Molecular microbiology

authors： Peters PC,Migocki MD,Thoni C,Harry EJ

更新日期：2007-04-01 00:00:00

abstract：:Small repeat sequences in bacterial genomes, which represent non-autonomous mobile elements, have close similarities to archaeon and eukaryotic miniature inverted repeat transposable elements. These repeat elements are found in both intergenic and intragenic chromosomal regions, and contain an array of diverse motifs....

journal_title：Molecular microbiology

authors： Delihas N

更新日期：2008-02-01 00:00:00

abstract：:Interactions between domains of ATP-binding cassette (ABC) transporters are of great functional importance and yet are poorly understood. To gain further knowledge of these protein-protein interactions, we studied the inner membrane complex of the maltose transporter of Escherichia coli. We focused on interactions bet...

journal_title：Molecular microbiology

authors： Mourez M,Jéhanno M,Schneider E,Dassa E

更新日期：1998-10-01 00:00:00

abstract：:A Rhizobium phaseoli cytochrome mutant, unable to oxidize N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD), was isolated after Mu-dl (Kan lac) mutagenesis of the wild-type strain CE-3. Mutant strain CFN4202 had sixfold less haem-c but similar levels of b type, o and aa3 cytochromes than the wild-type strain. CFN4202 st...

journal_title：Molecular microbiology

authors： Soberón M,Aguilar GR,Sánchez F

更新日期：1993-04-01 00:00:00

abstract：:The malaria parasite Plasmodium falciparum assembles knob structures underneath the erythrocyte membrane that help present the major virulence protein, P. falciparum erythrocyte membrane protein-1 (PfEMP1). Membranous structures called Maurer's clefts are established in the erythrocyte cytoplasm and function as sortin...

journal_title：Molecular microbiology

authors： Dixon MW,Kenny S,McMillan PJ,Hanssen E,Trenholme KR,Gardiner DL,Tilley L

更新日期：2011-08-01 00:00:00

abstract：:The DNA damage and stress response pathways interact to regulate cellular responses to genotoxins and environmental stresses. How these pathways interact in Schizosaccharomyces pombe is not well understood. We demonstrate that osmotic stress suppresses the DNA damage sensitivity of checkpoint mutants, and that this oc...

journal_title：Molecular microbiology

authors： Alao JP,Huis In 't Veld PJ,Buhse F,Sunnerhagen P

更新日期：2010-07-01 00:00:00

abstract：:Tuberculosis remains the greatest cause of death worldwide due to a single pathogen. In order to identify the genes required for the pathogenicity of Mycobacterium tuberculosis, a functional genomic approach was developed. A library of signature-tagged transposon mutants of this bacterium was constructed and screened ...

journal_title：Molecular microbiology

authors： Camacho LR,Ensergueix D,Perez E,Gicquel B,Guilhot C

更新日期：1999-10-01 00:00:00

abstract：:Protection against maternal malaria has been associated with the acquisition of a specific antibody response that prevents adhesion of Plasmodium falciparum-infected erythrocytes to the glycosaminoglycan chondroitin-4-sulphate (CSA), which is present in the placental intervillous space. These antibodies are directed a...

journal_title：Molecular microbiology

authors： Andrews KT,Pirrit LA,Przyborski JM,Sanchez CP,Sterkers Y,Ricken S,Wickert H,Lépolard C,Avril M,Scherf A,Gysin J,Lanzer M

更新日期：2003-08-01 00:00:00

abstract：:We used artificial chromosome inversions to investigate the chromosomal constraints that preserve genome organization in the Gram-positive bacterium Lactococcus lactis. Large inversions, 80-1260 kb in length, disturbing the symmetry of the origin and terminus of the replication axis to various extents, were constructe...

journal_title：Molecular microbiology

authors： Campo N,Dias MJ,Daveran-Mingot ML,Ritzenthaler P,Le Bourgeois P

更新日期：2004-01-01 00:00:00

abstract：:Bacteria react to phosphate starvation by activating genes involved in the transport and assimilation of phosphate as well as other phosphorous compounds. Some soil bacteria have evolved an additional mechanism for saving phosphorous. Under phosphate-limiting conditions, they replace their membrane phospholipids by li...

journal_title：Molecular microbiology

authors： Geiger O,Röhrs V,Weissenmayer B,Finan TM,Thomas-Oates JE

更新日期：1999-04-01 00:00:00

abstract：:Proteus mirabilis is a common cause of urinary tract infection (UTI) in individuals with structural abnormalities or long-term catheterization. The expression of mannose-resistant/Proteus-like (MR/P) fimbria is phase variable because of the inversion of a 251 bp DNA fragment that carries the promoter for the mrp opero...

journal_title：Molecular microbiology

authors： Li X,Lockatell CV,Johnson DE,Mobley HL

更新日期：2002-08-01 00:00:00

abstract：:Site-specific recombination by the Cre recombinase takes place at a simple DNA site (loxP), requires no additional proteins and gives topologically simple recombination products. In contrast, cer and psi sites for Xer recombination contain approximately 150 bp of accessory sequences, require accessory proteins PepA, A...

journal_title：Molecular microbiology

authors： Gourlay SC,Colloms SD

更新日期：2004-04-01 00:00:00

abstract：:The DnaA protein, which initiates chromosomal replication in Escherichia coli, is negatively regulated by both the sliding clamp of DNA polymerase III holoenzyme and the IdaB protein. We have found that, when the amount of minichromosome is limited in an in vitro replication system, minichromosomal replication-stimula...

journal_title：Molecular microbiology

authors： Su'etsugu M,Kawakami H,Kurokawa K,Kubota T,Takata M,Katayama T

更新日期：2001-04-01 00:00:00

abstract：:The two-component regulatory system PhoPQ has been shown to regulate the expression of virulence factors in a number of bacterial species. For one such virulence factor, lipopolysaccharide (LPS), the PhoPQ system has been shown to regulate structural modifications in Salmonella enterica var Typhimurium. In Yersinia pe...

journal_title：Molecular microbiology

authors： Hitchen PG,Prior JL,Oyston PC,Panico M,Wren BW,Titball RW,Morris HR,Dell A

更新日期：2002-06-01 00:00:00

abstract：:Borrelia burgdorferi, the Lyme disease agent, has double-stranded linear plasmids with covalently closed ends. DNA at the ends, or telomeres, of two linear plasmids of B. burgdorferi strain B31 was examined. Telomeric sequences from both ends of a 16 kb linear plasmid and from one end of a 49 kb linear plasmid were cl...

journal_title：Molecular microbiology

authors： Hinnebusch J,Bergström S,Barbour AG

更新日期：1990-05-01 00:00:00

abstract：:IscU is a central component of the ISC machinery and serves as a scaffold for de novo assembly of Fe-S clusters. The dedicated chaperone system composed of the Hsp70-chaperone HscA and the J-protein cochaperone HscB synergistically interacts with IscU and facilitates cluster transfer from IscU to recipient apo-protein...

journal_title：Molecular microbiology

authors： Sato S,Matsushima Y,Kanazawa M,Tanaka N,Fujishiro T,Kunichika K,Nakamura R,Tomioka H,Wada K,Takahashi Y

更新日期：2020-11-17 00:00:00

abstract：:The cyanobacterium Anabaena responds to nitrogen deprivation by producing heterocysts, cells specialized for nitrogen fixation, at well-spaced intervals along its filaments. The gene hepA, required for heterocyst maturation, is expressed in response to nitrogen deprivation, prior to visible differentiation. A spatial ...

journal_title：Molecular microbiology

authors： Wolk CP,Elhai J,Kuritz T,Holland D

更新日期：1993-02-01 00:00:00

abstract：:To establish a lysogenic lifestyle, the temperate bacteriophage φC31 integrates its genome into the chromosome of its Streptomyces host, by site-specific recombination between attP (the attachment site in the phage DNA) and attB (the chromosomal attachment site). This reaction is promoted by a phage-encoded serine rec...

journal_title：Molecular microbiology

authors： Stark WM

更新日期：2011-06-01 00:00:00

abstract：:The formation of biofilm results in a major lifestyle switch that is thought to affect the expression of multiple genes and operons. We used DNA arrays to study the global effect of biofilm formation on gene expression in mature Escherichia coli K-12 biofilm. We show that, when biofilm is compared with the exponential...

journal_title：Molecular microbiology

authors： Beloin C,Valle J,Latour-Lambert P,Faure P,Kzreminski M,Balestrino D,Haagensen JA,Molin S,Prensier G,Arbeille B,Ghigo JM

更新日期：2004-02-01 00:00:00

abstract：:Malaria transmission requires that the parasites differentiate into gametocytes prior to ingestion by a mosquito during a blood meal. Once in the mosquito midgut the gametocytes emerge from red blood cells (RBCs), fertilize, develop into ookinetes and finally infectious sporozoites. Gamete surface antigen, Pfs230, is ...

journal_title：Molecular microbiology

authors： Eksi S,Czesny B,van Gemert GJ,Sauerwein RW,Eling W,Williamson KC

更新日期：2006-08-01 00:00:00

abstract：:Differential gene expression is a key strategy adopted by the Lyme disease spirochaete, Borrelia burgdorferi, for adaptation and survival in the mammalian host and the tick vector. Many B. burgdorferi surface lipoproteins fall into two distinct groups according to their expression patterns: one group primarily express...

journal_title：Molecular microbiology

authors： Wang P,Dadhwal P,Cheng Z,Zianni MR,Rikihisa Y,Liang FT,Li X

更新日期：2013-09-01 00:00:00

abstract：:TonB protein functions as an energy transducer, coupling cytoplasmic membrane electrochemical potential to the active transport of vitamin B12 and Fe(III)-siderophore complexes across the outer membrane of Escherichia coli and other Gram-negative bacteria. Accumulated evidence indicates that TonB is anchored in the cy...

journal_title：Molecular microbiology

authors： Larsen RA,Wood GE,Postle K

更新日期：1993-12-01 00:00:00

abstract：:The bacterial flagellar motor accommodates ten stator units around the rotor to produce large torque at high load. But when external load is low, some previous studies showed that a single stator unit can spin the rotor at the maximum speed, suggesting that the maximum speed does not depend on the number of active sta...

journal_title：Molecular microbiology

authors： Nakamura S,Hanaizumi Y,Morimoto YV,Inoue Y,Erhardt M,Minamino T,Namba K

更新日期：2020-04-01 00:00:00

abstract：:We previously characterized a defective-folding mutant of maltose-binding protein of Escherichia coli, MalE31, which formed periplasmic inclusion bodies. Here, we show that MalE31 aggregation does not affect bacterial growth at 30 degrees C but is lethal at 37 degrees C. Surprisingly, under mild heat shock conditions ...

journal_title：Molecular microbiology

authors： Hunke S,Betton JM

更新日期：2003-12-01 00:00:00

abstract：:The genes encoding the three terminal enzymes in the threonine biosynthetic pathway, homoserine dehydrogenase (hom), homoserine kinase (thrB) and threonine synthase (thrC) have been isolated from Corynebacterium glutamicum. The C. glutamicum hom and thrB genes were subcloned on a 3.6 kb SalI-generated chromosomal frag...

journal_title：Molecular microbiology

authors： Follettie MT,Shin HK,Sinskey AJ

更新日期：1988-01-01 00:00:00

abstract：:The csiD-ygaF-gabDTP region in the Escherichia coli genome represents a cluster of sigma S-controlled genes. Here, we investigated promoter structures, sigma factor dependencies, potential co-regulation and environmental regulatory patterns for all of these genes. We find that this region constitutes a complex operon ...

journal_title：Molecular microbiology

authors： Metzner M,Germer J,Hengge R

更新日期：2004-02-01 00:00:00

abstract：:The DNA sequence of the structural gene (yopE) of one of the Yersinia pseudotuberculosis virulence plas-mid-plB1-encoded proteins, Yop5, is presented. The deduced protein showed a molecular weight of 22971 Daltons. A specific mutant, having a kanamycin-resistance fragment inserted within the yopE gene was no longer vi...

journal_title：Molecular microbiology

authors： Forsberg A,Wolf-Watz H

更新日期：1988-01-01 00:00:00

abstract：:An internal segment of the (p)ppGpp synthetase gene, relA, of Streptomyces coelicolor A3(2) was amplified from genomic DNA using the polymerase chain reaction and used as a hybridization probe to isolate the complete gene from a cosmid library. relA lies downstream of a gene (apt) that apparently encodes adenine phosp...

journal_title：Molecular microbiology

authors： Chakraburtty R,White J,Takano E,Bibb M

更新日期：1996-01-01 00:00:00

abstract：:We report here the cloning of the arginine repressor gene argR of Bacillus stearothermophilus and the characterization and purification to homogeneity of its product. The deduced amino acid sequence of the 16.8-kDa ArgR subunit shares 72% identity with its mesophilic homologue AhrC of Bacilus subtilis. Sequence analys...

journal_title：Molecular microbiology

authors： Dion M,Charlier D,Wang H,Gigot D,Savchenko A,Hallet JN,Glansdorff N,Sakanyan V

更新日期：1997-07-01 00:00:00