DNA replication-coupled inactivation of DnaA protein in vitro: a role for DnaA arginine-334 of the AAA+ Box VIII motif in ATP hydrolysis.

abstract：:The actin-like protein FtsA is present in many eubacteria, and genetic experiments have shown that it plays an important, sometimes essential, role in cell division. Here, we show that Bacillus subtilis FtsA is targeted to division sites in both vegetative and sporulating cells. As in other organisms FtsA is probably ...

journal_title：Molecular microbiology

authors： Feucht A,Lucet I,Yudkin MD,Errington J

更新日期：2001-04-01 00:00:00

abstract：:The genetic and biochemical mechanisms by which Mycobacterium tuberculosis senses and responds to the complex environment that it encounters during infection and persistence within the host remain unknown. In a number of bacterial species, the Kdp signal transduction pathway appears to be the primary response to envir...

journal_title：Molecular microbiology

authors： Steyn AJ,Joseph J,Bloom BR

更新日期：2003-02-01 00:00:00

abstract：:The S box transcription termination control system regulates expression of genes involved in methionine metabolism. Expression of the S box regulon, comprised of 11 transcriptional units in Bacillus subtilis, is induced in response to starvation for methionine. We previously demonstrated that S-adenosylmethionine (SAM...

journal_title：Molecular microbiology

authors： McDaniel BA,Grundy FJ,Henkin TM

更新日期：2005-08-01 00:00:00

abstract：:We used artificial chromosome inversions to investigate the chromosomal constraints that preserve genome organization in the Gram-positive bacterium Lactococcus lactis. Large inversions, 80-1260 kb in length, disturbing the symmetry of the origin and terminus of the replication axis to various extents, were constructe...

journal_title：Molecular microbiology

authors： Campo N,Dias MJ,Daveran-Mingot ML,Ritzenthaler P,Le Bourgeois P

更新日期：2004-01-01 00:00:00

abstract：:Renewed interest in gene amplification stems from its importance in evolution and a variety of medical problems ranging from drug resistance to cancer. However, amplified DNA segments (amplicons) are not fully characterized in any organism. Here we report a novel Acinetobacter baylyi system for genome-wide studies. Am...

journal_title：Molecular microbiology

authors： Seaton SC,Elliott KT,Cuff LE,Laniohan NS,Patel PR,Neidle EL

更新日期：2012-02-01 00:00:00

abstract：:Restart of arrested replication forks is an important process and PriA, the main Escherichia coli replication restart protein, is essential for viability under any condition that increases the frequency of fork arrest. In priA mutant, replication forks are arrested by spontaneously occurring roadblocks and blocked rep...

journal_title：Molecular microbiology

authors： Grompone G,Sanchez N,Dusko Ehrlich S,Michel B

更新日期：2004-04-01 00:00:00

abstract：:Like other bacterial species, Mycobacterium tuberculosis has multiple sigma (sigma) factors encoded in its genome. In previously published work, we and others have shown that mutations in some of these transcriptional activators render M. tuberculosis sensitive to various environmental stresses and, in some cases, cau...

journal_title：Molecular microbiology

authors： Manganelli R,Voskuil MI,Schoolnik GK,Dubnau E,Gomez M,Smith I

更新日期：2002-07-01 00:00:00

abstract：:Recently, sexual development in the heterothallic ascomycete Trichoderma reesei (anamorph of Hypocrea jecorina) has been achieved and thus initiated attempts to elucidate regulation and determinants of this process. While the α-type pheromone of this fungus fits the consensus known from other fungi, the assumed a-type...

journal_title：Molecular microbiology

authors： Schmoll M,Seibel C,Tisch D,Dorrer M,Kubicek CP

更新日期：2010-09-01 00:00:00

abstract：:5'-nucleotidases (5'-NTs) are enzymes that catalyze the hydrolysis of nucleoside monophosphates to produce nucleosides and phosphate. Since the identification of adenosine synthase A (AdsA) in Staphylococcus aureus in 2009, several other 5'-NTs have been discovered in Gram-positive cocci, mainly in streptococci. Despi...

journal_title：Molecular microbiology

authors： Soh KY,Loh JMS,Proft T

更新日期：2020-04-01 00:00:00

abstract：:Tst, the gene for toxic shock syndrome toxin-1 (TSST-1), is part of a 15.2 kb genetic element in Staphylococcus aureus that is absent in TSST-1-negative strains. The prototype, in RN4282, is flanked by a 17 nucleotide direct repeat and contains genes for a second possible superantigen toxin, a Dichelobacter nodosus Va...

journal_title：Molecular microbiology

authors： Lindsay JA,Ruzin A,Ross HF,Kurepina N,Novick RP

更新日期：1998-07-01 00:00:00

abstract：:The bacterial enhancer binding proteins (bEBP) are members of the AAA+ protein family and have a highly conserved 'DE' Walker B motif thought to be involved in the catalytic function of the protein with an active role in nucleotide hydrolysis. Based on detailed structural data, we analysed the functionality of the con...

journal_title：Molecular microbiology

authors： Joly N,Rappas M,Wigneshweraraj SR,Zhang X,Buck M

更新日期：2007-11-01 00:00:00

abstract：:Holins control the length of the infection cycle of tailed phages (the Caudovirales) by oligomerizing to form lethal holes in the cytoplasmic membrane at a time dictated by their primary structure. Nothing is currently known about the physical basis of their oligomerization or the structure of the oligomers formed by ...

journal_title：Molecular microbiology

authors： Savva CG,Dewey JS,Deaton J,White RL,Struck DK,Holzenburg A,Young R

更新日期：2008-08-01 00:00:00

abstract：:A decline in our ability to successfully treat patients with malaria infections of the parasitic protozoan Plasmodium falciparum with cheap quinoline drugs has led to a huge escalation in morbidity and mortality in recent years. Many approaches have been taken, including classical genetics, reverse genetics and molecu...

journal_title：Molecular microbiology

authors： Duraisingh MT,Refour P

更新日期：2005-08-01 00:00:00

abstract：:A number of strains of the widespread aerobic soil bacterium, Bacillus sphaericus, possess crystalline inclusions of a toxin lethal to a variety of insect (larvae) which are vectors of major tropical diseases. Partial amino acid sequence data from one strain, B. sphaericus 2362 have permitted us to design oligonucleot...

journal_title：Molecular microbiology

authors： Hindley J,Berry C

更新日期：1987-09-01 00:00:00

abstract：:Corynebacterium glutamicum is a Gram-positive soil bacterium that prefers the simultaneous catabolism of different carbon sources rather than their sequential utilization. This type of metabolism requires an adaptation of the utilization rates to the overall metabolic capacity. Here we show how two functionally redund...

journal_title：Molecular microbiology

authors： Frunzke J,Engels V,Hasenbein S,Gätgens C,Bott M

更新日期：2008-01-01 00:00:00

abstract：:Bacteria use quorum-sensing signals or autoinducers to communicate. The signals in Gram-positive bacteria are often peptides activated by proteolytic removal of an N-terminal leader sequence. While investigating stimulation of antimicrobial peptide production by the Streptococcus mutans synthetic competence stimulatin...

journal_title：Molecular microbiology

authors： Petersen FC,Fimland G,Scheie AA

更新日期：2006-09-01 00:00:00

abstract：:The cyanobacterium Anabaena responds to nitrogen deprivation by producing heterocysts, cells specialized for nitrogen fixation, at well-spaced intervals along its filaments. The gene hepA, required for heterocyst maturation, is expressed in response to nitrogen deprivation, prior to visible differentiation. A spatial ...

journal_title：Molecular microbiology

authors： Wolk CP,Elhai J,Kuritz T,Holland D

更新日期：1993-02-01 00:00:00

abstract：:We used DNA macroarray analysis to identify genes that respond to the status of the intracellular acetyl phosphate (acP) pool. Genes whose expression correlated negatively with the ability to synthesize acP (i.e. negatively regulated genes) function primarily in flagella biosynthesis, a result consistent with observat...

journal_title：Molecular microbiology

authors： Wolfe AJ,Chang DE,Walker JD,Seitz-Partridge JE,Vidaurri MD,Lange CF,Prüss BM,Henk MC,Larkin JC,Conway T

更新日期：2003-05-01 00:00:00

abstract：:Filamentous hemagglutinin (FHA) is a critically important virulence factor produced by Bordetella species that cause respiratory infections in humans and other animals. It is also a prototypical member of the widespread two partner secretion (TPS) pathway family of proteins. First synthesized as a ~370 kDa protein cal...

journal_title：Molecular microbiology

authors： Nash ZM,Cotter PA

更新日期：2019-09-01 00:00:00

abstract：:Visual inspection showed clear evidence of a history of intraspecies recombinational exchanges within the neighbouring meningococcal shikimate dehydrogenase (aroE) and glutamine synthetase (glnA) genes, which was supported by the non-congruence of the trees constructed from the sequences of these genes from different ...

journal_title：Molecular microbiology

authors： Zhou J,Bowler LD,Spratt BG

更新日期：1997-02-01 00:00:00

abstract：:Initiation and re-initiation of chromosomal DNA replication in bacteria rely on divergent multiprotein assemblies, which direct the functional delivery of the replicative helicase on single-stranded DNA (ssDNA) at specific sites. These two processes are triggered either at the single chromosomal origin oriC or at arre...

journal_title：Molecular microbiology

authors： Bruand C,Velten M,McGovern S,Marsin S,Sérèna C,Ehrlich SD,Polard P

更新日期：2005-02-01 00:00:00

abstract：:The cytochrome c nitrite reductase complex (NrfHA) is the terminal enzyme in the electron transport chain catalysing nitrite respiration of Wolinella succinogenes. The catalytic subunit NrfA is a pentahaem cytochrome c containing an active site haem group which is covalently bound via the cysteine residues of a unique...

journal_title：Molecular microbiology

authors： Pisa R,Stein T,Eichler R,Gross R,Simon J

更新日期：2002-02-01 00:00:00

abstract：:To identify Rhodobacter capsulatus nif genes necessary for the alternative nitrogenase, strains carrying defined mutations in 32 genes and open reading frames of nif region A, B or C were constructed. The ability of these mutants to grow on nitrogen-free medium with molybdenum (Nif phenotype) or in a nifHDK deletion b...

journal_title：Molecular microbiology

authors： Schüddekopf K,Hennecke S,Liese U,Kutsche M,Klipp W

更新日期：1993-05-01 00:00:00

abstract：:Type IV secretion systems are virulence determinants in many bacteria and share extensive homology with many conjugal transfer systems. Although type IV systems and their homologues have been studied widely, the mechanism by which substrates are secreted remains unclear. In Agrobacterium, we show that type IV secretio...

journal_title：Molecular microbiology

authors： Pantoja M,Chen L,Chen Y,Nester EW

更新日期：2002-09-01 00:00:00

abstract：:Two distinct merR genes, which regulate expression of the mercuric ion resistance gene (mer), of Thiobacillus ferrooxidans strain E-15 have been cloned, sequenced and termed merR1 and merR2. As a result of gene walking around two merR genes, it was found that these two genes were quite close in distance. The nucleotid...

journal_title：Molecular microbiology

authors： Inoue C,Sugawara K,Kusano T

更新日期：1991-11-01 00:00:00

abstract：:Polymorphonuclear neutrophils are the most important mammalian host defence cells against infections with Pseudomonas aeruginosa. Screening of a signature tagged mutagenesis library of the non-piliated P. aeruginosa strain TBCF10839 uncovered that transposon inactivation of its pilY1 gene rendered the bacterium more r...

journal_title：Molecular microbiology

authors： Bohn YS,Brandes G,Rakhimova E,Horatzek S,Salunkhe P,Munder A,van Barneveld A,Jordan D,Bredenbruch F,Häussler S,Riedel K,Eberl L,Jensen PØ,Bjarnsholt T,Moser C,Hoiby N,Tümmler B,Wiehlmann L

更新日期：2009-02-01 00:00:00

abstract：:In vivo and in vitro analyses indicate that transcription of the argO gene coding for an arginine exporter is regulated by the global transcriptional regulator Lrp, an effect that went by unnoticed in previous genome-scale screenings of the Lrp regulatory network in Escherichia coli. Lrp activates the argO promoter fo...

journal_title：Molecular microbiology

authors： Peeters E,Nguyen Le Minh P,Foulquié-Moreno M,Charlier D

更新日期：2009-12-01 00:00:00

abstract：:The var multicopy gene family encodes Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) variant antigens, which, through their ability to adhere to a variety of host receptors, are thought to be important virulence factors. The predominant expression of a single cytoadherent PfEMP1 type on an infected red ...

journal_title：Molecular microbiology

authors： Kyes SA,Christodoulou Z,Raza A,Horrocks P,Pinches R,Rowe JA,Newbold CI

更新日期：2003-06-01 00:00:00

abstract：:The GATA factor AreA is a wide-domain regulator in Aspergillus nidulans with transcriptional activation and chromatin remodelling functions. AreA interacts with the nitrate-specific Zn(2)-C(6) cluster protein NirA and both proteins cooperate to synergistically activate nitrate-responsive genes. We have previously esta...

journal_title：Molecular microbiology

authors： Berger H,Pachlinger R,Morozov I,Goller S,Narendja F,Caddick M,Strauss J

更新日期：2006-01-01 00:00:00

abstract：:The cGvpE protein of Halobacterium salinarum PHH4 has been identified as transcriptional activator for the promoter of the c-gvpA gene encoding the major gas vesicle structural protein cGvpA. Molecular modelling of the carboxy-terminal region of cGvpE suggests that this protein resembles a basic leucine-zipper protein...

journal_title：Molecular microbiology

authors： Plösser P,Pfeifer F

更新日期：2002-07-01 00:00:00