Multiple roles of Pseudomonas aeruginosa TBCF10839 PilY1 in motility, transport and infection.

abstract：:Disulfide bonds are found in many proteins associated with the cell wall of Escherichia coli, and for some of these proteins the disulfide bond is critical to their stability and function. One protein found to contain a disulfide bond is the essential cell division protein FtsN, but the importance of this bond to the ...

journal_title：Molecular microbiology

authors： Meehan BM,Landeta C,Boyd D,Beckwith J

更新日期：2017-02-01 00:00:00

abstract：:The threonine dehydratase IlvA is part of the isoleucine biosynthesis pathway in the Gram-positive model bacterium Bacillus subtilis. Consequently, deletion of ilvA causes isoleucine auxotrophy. It has been reported that ilvA pseudo-revertants having a derepressed hom-thrCB operon appear in the presence of threonine. ...

journal_title：Molecular microbiology

authors： Rosenberg J,Müller P,Lentes S,Thiele MJ,Zeigler DR,Tödter D,Paulus H,Brantl S,Stülke J,Commichau FM

更新日期：2016-09-01 00:00:00

abstract：:An operon including two new genes (nasS and nasT) has been defined, cloned and sequenced. The deduced NASS protein is homologous to NRTA from Synechococcus sp. and to NASF from Klebsiella pneumoniae, two proteins involved in nitrate uptake. The predicted NAST polypeptide is homologous to the regulator proteins of the ...

journal_title：Molecular microbiology

authors： Gutierrez JC,Ramos F,Ortner L,Tortolero M

更新日期：1995-11-01 00:00:00

abstract：:A 2.6 kb ClaI-BamHI DNA fragment of megaplasmid 2 of Rhizobium meliloti 2011 was found to carry genes involved in exopolysaccharide synthesis and infection of alfalfa nodules. The analysis of the nucleotide sequence of this DNA fragment revealed the existence of two open reading frames (ORFs) running in opposite direc...

journal_title：Molecular microbiology

authors： Buendia AM,Enenkel B,Köplin R,Niehaus K,Arnold W,Pühler A

更新日期：1991-06-01 00:00:00

abstract：:A 4.0 kb EcoRI fragment of Bacillus subtilis conferring thiostrepton resistance was cloned and characterized. By nucleotide sequencing of the relevant region, six open reading frames were established, which corresponded to a part of spo0H, a ribosomal protein gene (rpmG), an unidentified open reading frame (orfE), a t...

journal_title：Molecular microbiology

authors： Jeong SM,Yoshikawa H,Takahashi H

更新日期：1993-10-01 00:00:00

abstract：:Eukaryotic cells have evolved a complex set of intracellular organelles, each of which possesses a specific complement of enzymes and performs unique metabolic functions. This compartmentalization of cellular functions provides a level of metabolic control not available to prokaryotes. However, it presents the eukaryo...

journal_title：Molecular microbiology

authors： Aitchison JD,Nuttley WM,Szilard RK,Brade AM,Glover JR,Rachubinski RA

更新日期：1992-12-01 00:00:00

abstract：:ICP is a chagasin-family natural tight binding inhibitor of Clan CA, family C1 cysteine peptidases (CPs). We investigated the role of ICP in Trypanosoma brucei by generating bloodstream form ICP-deficient mutants (Deltaicp). A threefold increase in CP activity was detected in lysates of Deltaicp, which was restored to...

journal_title：Molecular microbiology

authors： Santos CC,Coombs GH,Lima AP,Mottram JC

更新日期：2007-11-01 00:00:00

abstract：:Prokaryotes protect their genomes from foreign DNA with a diversity of defence mechanisms, including a widespread restriction-modification (R-M) system involving phosphorothioate (PT) modification of the DNA backbone. Unlike classical R-M systems, highly partial PT modification of consensus motifs in bacterial genomes...

journal_title：Molecular microbiology

authors： Cao B,Cheng Q,Gu C,Yao F,DeMott MS,Zheng X,Deng Z,Dedon PC,You D

更新日期：2014-08-01 00:00:00

abstract：:Invasive sporozoite and merozoite stages of malaria parasites that infect mammals enter and subsequently reside in hepatocytes and red blood cells respectively. Each invasive stage may exhibit unique adaptations that allow it to interact with and survive in its distinct host cell environment, and these adaptations are...

journal_title：Molecular microbiology

authors： Kaiser K,Matuschewski K,Camargo N,Ross J,Kappe SH

更新日期：2004-03-01 00:00:00

abstract：:The DnaA protein, which initiates chromosomal replication in Escherichia coli, is negatively regulated by both the sliding clamp of DNA polymerase III holoenzyme and the IdaB protein. We have found that, when the amount of minichromosome is limited in an in vitro replication system, minichromosomal replication-stimula...

journal_title：Molecular microbiology

authors： Su'etsugu M,Kawakami H,Kurokawa K,Kubota T,Takata M,Katayama T

更新日期：2001-04-01 00:00:00

abstract：:The ctaD gene encoding subunit I of the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides has been cloned. The gene encodes a polypeptide of 565 residues which is highly homologous to the sequences of subunit I from other prokaryotic and eukaryotic sources, e.g. 51% identity with that from bovine, and 75% ide...

journal_title：Molecular microbiology

authors： Shapleigh JP,Gennis RB

更新日期：1992-03-01 00:00:00

abstract：:The formation of biofilm results in a major lifestyle switch that is thought to affect the expression of multiple genes and operons. We used DNA arrays to study the global effect of biofilm formation on gene expression in mature Escherichia coli K-12 biofilm. We show that, when biofilm is compared with the exponential...

journal_title：Molecular microbiology

authors： Beloin C,Valle J,Latour-Lambert P,Faure P,Kzreminski M,Balestrino D,Haagensen JA,Molin S,Prensier G,Arbeille B,Ghigo JM

更新日期：2004-02-01 00:00:00

abstract：:The Ca(2+)-dependent protein phosphatase calcineurin is an important regulator of ion transporters from many organisms, including the Saccharomyces cerevisiae vacuolar Ca(2+)/H(+) exchanger Vcx1p. In yeast and plants, cation/H(+) exchangers are important in shaping cytosolic Ca(2+) levels involved in signal transducti...

journal_title：Molecular microbiology

authors： Pittman JK,Cheng NH,Shigaki T,Kunta M,Hirschi KD

更新日期：2004-11-01 00:00:00

abstract：:To investigate the determining factors in the selection of the transcription start points (tsp) by RNA polymerase of Escherichia coli, we systematically deleted or substituted single base pairs (bps) at 25 putative critical positions in the two extended -10 promoters, P1 and P2, of the gal operon. These changes extend...

journal_title：Molecular microbiology

authors： Lewis DE,Adhya S

更新日期：2004-11-01 00:00:00

abstract：:Prior studies have shown that the catalase-deficient pathogen Streptococcus pyogenes (group A streptococcus) has a robust ability to resist oxidative stress that partially involves the transcriptional regulator PerR. However, the extent of the PerR regulon and the contribution of the members of this regulon to virulen...

journal_title：Molecular microbiology

authors： Brenot A,King KY,Caparon MG

更新日期：2005-01-01 00:00:00

abstract：:In Candida glabrata, the sirtuins Sir2 and Hst1 control the expression of a wide number of genes including adhesins required for host colonization and niacin transporters needed for growth. Given that these sirtuins can be inactivated during infection, we asked if their inhibition could modify the response of C. glabr...

journal_title：Molecular microbiology

authors： Orta-Zavalza E,Guerrero-Serrano G,Gutiérrez-Escobedo G,Cañas-Villamar I,Juárez-Cepeda J,Castaño I,De Las Peñas A

更新日期：2013-06-01 00:00:00

abstract：:Cell-cell and cell-surface adherence represents initial steps in forming multicellular aggregates or in establishing cell-surface interactions. The commonly used Saccharomyces cerevisiae laboratory strain S288c carries a flo8 mutation, and is only able to express the flocculin-encoding genes FLO1 and FLO11, when FLO8 ...

journal_title：Molecular microbiology

authors： Fichtner L,Schulze F,Braus GH

更新日期：2007-12-01 00:00:00

abstract：:We have isolated and sequenced a set of deletions stimulated by DNA palindromes in Escherichia coli. All of the deletions are asymmetric with respect to the parental sequence and have occurred at short direct repeats. This is consistent with deletion by strand slippage during DNA replication. The orientation of the as...

journal_title：Molecular microbiology

authors： Pinder DJ,Blake CE,Lindsey JC,Leach DR

更新日期：1998-05-01 00:00:00

abstract：:The diffusible factor synthase XanB2, originally identified in Xanthomonas campestris pv. campestris (Xcc), is highly conserved across a wide range of bacterial species, but its substrate and catalytic mechanism have not yet been investigated. Here, we show that XanB2 is a unique bifunctional chorismatase that hydroly...

journal_title：Molecular microbiology

authors： Zhou L,Wang JY,Wu J,Wang J,Poplawsky A,Lin S,Zhu B,Chang C,Zhou T,Zhang LH,He YW

更新日期：2013-01-01 00:00:00

abstract：:The enteric gamma-proteobacterium Photorhabdus luminescens kills a wide range of insects, whilst also maintaining a mutualistic relationship with soil nematodes from the family Heterorhabditis. Pathogenicity is associated with bacterial exponential growth, whilst mutualism is associated with post-exponential (stationa...

journal_title：Molecular microbiology

authors： Bager R,Roghanian M,Gerdes K,Clarke DJ

更新日期：2016-05-01 00:00:00

abstract：:Advances in automated fluorescence microscopy have made snapshot and time-lapse imaging of bacterial cells commonplace, yet fundamental challenges remain in analysis. The vast quantity of data collected in high-throughput experiments requires a fast and reliable automated method to analyze fluorescence intensity and l...

journal_title：Molecular microbiology

authors： Cass JA,Stylianidou S,Kuwada NJ,Traxler B,Wiggins PA

更新日期：2017-03-01 00:00:00

abstract：:Expression of the gene pcnB, encoding the dispensable Escherichia coli poly(A) polymerase (PAPI), which is toxic when overproduced, was investigated. Its promoter was identified and found to be moderately strong when used to express a beta-galactosidase reporter. Expression levels were not affected by increasing or de...

journal_title：Molecular microbiology

authors： Binns N,Masters M

更新日期：2002-06-01 00:00:00

abstract：:Motile bacteria sense their physical and chemical environment through highly cooperative, ordered arrays of chemoreceptors. These signalling complexes phosphorylate a response regulator which in turn governs flagellar motor reversals, driving cells towards favourable environments. The structural changes that translate...

journal_title：Molecular microbiology

authors： Briegel A,Ames P,Gumbart JC,Oikonomou CM,Parkinson JS,Jensen GJ

更新日期：2013-09-01 00:00:00

abstract：:The ro mutants of Neurospora crassa are defective in nuclear migration and hyphal morphogenesis. Several of the ro loci have recently been shown to encode components of the dynein/dynactin motor complex. Here we report on the cloning and characterization of the ro-2 gene which codes for a novel 80 kDa protein that has...

journal_title：Molecular microbiology

authors： Vierula PJ,Mais JM

更新日期：1997-04-01 00:00:00

abstract：:The unfolded protein response (UPR) is an important stress signalling pathway involved in the cellular development and environmental adaptation of fungi. We investigated the importance of the UPR pathway in the pathogenicity of the plant necrotrophic fungus Alternaria brassicicola, which causes black spot disease on a...

journal_title：Molecular microbiology

authors： Joubert A,Simoneau P,Campion C,Bataillé-Simoneau N,Iacomi-Vasilescu B,Poupard P,François JM,Georgeault S,Sellier E,Guillemette T

更新日期：2011-03-01 00:00:00

abstract：:A new locus required for type 4 pilus biogenesis by Pseudomonas aeruginosa has been identified. A pilE mutant, designated MJ-6, was broadly resistant to pili-specific phages and unable to translocate across solid surfaces by the pilus-dependent mechanism of twitching motility (Twt-). Immunoblot analysis demonstrated t...

journal_title：Molecular microbiology

authors： Russell MA,Darzins A

更新日期：1994-09-01 00:00:00

abstract：:The 136 codon (408 bp) denA gene encoding endonuclease II (Endoll) of bacteriophage T4 was unambiguously identified through sequencing and subsequent cloning. Endoll prepared from cloned DNA through coupled in vitro transcription-translation nicked and cut DNA in vitro in a sequence-specific manner. In vitro (and in v...

journal_title：Molecular microbiology

authors： Carlson K,Kosturko LD,Nyström AC

更新日期：1999-03-01 00:00:00

abstract：:DivIB, DivIC and FtsL are bacterial proteins essential for cell division, which show interdependencies for their stabilities and localization. We have reconstituted in vitro a trimeric complex consisting of the recombinant extracellular domains of the three proteins from Streptococcus pneumoniae. The extracellular dom...

journal_title：Molecular microbiology

authors： Noirclerc-Savoye M,Le Gouëllec A,Morlot C,Dideberg O,Vernet T,Zapun A

更新日期：2005-01-01 00:00:00

abstract：:Helicobacter pylori is a human gastric pathogen associated with gastric and duodenal ulcers as well as gastric cancer. Mounting evidence suggests this pathogen's motility is prerequisite for successful colonization of human gastric tissues. Here, we isolated an H. pylori G27 HP0518 mutant exhibiting altered motility i...

journal_title：Molecular microbiology

authors： Asakura H,Churin Y,Bauer B,Boettcher JP,Bartfeld S,Hashii N,Kawasaki N,Mollenkopf HJ,Jungblut PR,Brinkmann V,Meyer TF

更新日期：2010-12-01 00:00:00

abstract：:We have previously described HpuAB, a two-component receptor that mediates binding to haemoglobin (Hb), haemoglobin-haptoglobin (Hb-Hp) and apo-haptoglobin (Hp). In this communication, we constructed non-polar mutations in the hpuA and hpuB loci to examine the individual roles of HpuA and HpuB. Our results indicate th...

journal_title：Molecular microbiology

authors： Lewis LA,Gipson M,Hartman K,Ownbey T,Vaughn J,Dyer DW

更新日期：1999-06-01 00:00:00