Pathological phenotypes and in vivo DNA cleavage by unrestrained activity of a phosphorothioate-based restriction system in Salmonella.

abstract：:The human fungal pathogen Candida albicans switches its morphology from yeast to hyphal forms. The morphological transition may render C. albicans virulent. Several signaling cascades, including those of the cyclic AMP-protein kinase A and mitogen-activated protein kinase pathways, are responsible for morphogenesis. I...

journal_title：Molecular microbiology

authors： Kim SW,Joo YJ,Chun YJ,Park YK,Kim J

更新日期：2019-09-01 00:00:00

abstract：:Streptococcus lactis NCDO763 harbours a plasmid designated pLP763. The cells harbouring pLP763 are able to grow to a higher density in milk because of their proteinase-positive phenotype (Prt+). The 6.2 kb HindIII-PstI fragment from pLP763 was found to be responsible for the Prt+ phenotype. The DNA fragment contains a...

journal_title：Molecular microbiology

authors： Kiwaki M,Ikemura H,Shimizu-Kadota M,Hirashima A

更新日期：1989-03-01 00:00:00

abstract：:pAM beta 1 is a low-copy-number, promiscuous plasmid from Gram-positive bacteria that replicates by a unidirectional theta-type mode. Its replication is initiated by an original mechanism, involving the positive rate-limiting RepE protein. Here we show that the pAM beta 1-encoded CopF protein is involved in negative r...

journal_title：Molecular microbiology

authors： Le Chatelier E,Ehrlich SD,Jannière L

更新日期：1994-11-01 00:00:00

abstract：:The HPr kinase of Gram-positive bacteria is an ATP-dependent serine protein kinase, which phosphorylates the HPr protein of the bacterial phosphotransferase system (PTS) and is involved in the regulation of carbohydrate metabolism. The hprK gene from Enterococcus faecalis was cloned via polymerase chain reaction (PCR)...

journal_title：Molecular microbiology

authors： Kravanja M,Engelmann R,Dossonnet V,Blüggel M,Meyer HE,Frank R,Galinier A,Deutscher J,Schnell N,Hengstenberg W

更新日期：1999-01-01 00:00:00

abstract：:A transposon insertion screen implicated the yejH gene in the repair of ionizing radiation-induced damage. The yejH gene, which exhibits significant homology to the human transcription-coupled DNA repair gene XPB, is involved in the repair of double-strand DNA breaks. Deletion of yejH significantly sensitized cells to...

journal_title：Molecular microbiology

authors： Chen SH,Byrne RT,Wood EA,Cox MM

更新日期：2015-03-01 00:00:00

abstract：:The Plasmodium falciparum genome sequence has boosted hopes for a new era of malaria research and for the application of comprehensive molecular knowledge to disease control, but formidable obstacles remain: approximately 60% of the predicted P. falciparum proteins have no known functions or homologues, and most life ...

journal_title：Molecular microbiology

authors： Su XZ,Wootton JC

更新日期：2004-09-01 00:00:00

abstract：:In response to recommendations from the Steering Committees responsible for co-ordination of World Health Organization programmes for research on the immunology of leprosy (IMMLEP) and tuberculosis (IMMTUB), a list was prepared summarizing the properties of mycobacterial proteins currently under investigation with res...

journal_title：Molecular microbiology

authors： Young DB,Kaufmann SH,Hermans PW,Thole JE

更新日期：1992-01-01 00:00:00

abstract：:The rotA gene of Escherichia coli encodes a peptidyl-prolyl cis/trans isomerase (PPIase), which is supposed to catalyse protein folding in the periplasm. To investigate the importance of the enzyme, the rotA gene was cloned and a chromosomal deletion mutant was created. The rotA mutant was normally viable. No residual...

journal_title：Molecular microbiology

authors： Kleerebezem M,Heutink M,Tommassen J

更新日期：1995-10-01 00:00:00

abstract：:Holins control the length of the infection cycle of tailed phages (the Caudovirales) by oligomerizing to form lethal holes in the cytoplasmic membrane at a time dictated by their primary structure. Nothing is currently known about the physical basis of their oligomerization or the structure of the oligomers formed by ...

journal_title：Molecular microbiology

authors： Savva CG,Dewey JS,Deaton J,White RL,Struck DK,Holzenburg A,Young R

更新日期：2008-08-01 00:00:00

abstract：:Streptococcus mutans, a commensal of the human oral cavity, can survive in the bloodstream and cause infective endocarditis (IE). However, the virulence factors associated with this manifestation of disease are not known. Here, we demonstrate that AtlA, an autolysin of S. mutans is a newly identified fibronectin (Fn) ...

journal_title：Molecular microbiology

authors： Jung CJ,Zheng QH,Shieh YH,Lin CS,Chia JS

更新日期：2009-11-01 00:00:00

abstract：:Enteropathogenic Escherichia coli (EPEC) is a leading cause of infant diarrhoea. EPEC mediates several effects on host epithelial cells, including activation of signal-transduction pathways, cytoskeletal rearrangement along with pedestal and attaching/effacing lesion formation. It has been previously shown that the EP...

journal_title：Molecular microbiology

authors： Kenny B,Lai LC,Finlay BB,Donnenberg MS

更新日期：1996-04-01 00:00:00

abstract：:Differential expression of the genes expressing Pap pili in Escherichia coli was suggested to involve mRNAs with different stabilities. As the result of a post-transcriptional processing event, a papA gene-specific mRNA product (mRNA-A) accumulates in large excess relative to the primary mRNA-BA transcript. Our result...

journal_title：Molecular microbiology

authors： Nilsson P,Uhlin BE

更新日期：1991-07-01 00:00:00

abstract：:The actin-like protein FtsA is present in many eubacteria, and genetic experiments have shown that it plays an important, sometimes essential, role in cell division. Here, we show that Bacillus subtilis FtsA is targeted to division sites in both vegetative and sporulating cells. As in other organisms FtsA is probably ...

journal_title：Molecular microbiology

authors： Feucht A,Lucet I,Yudkin MD,Errington J

更新日期：2001-04-01 00:00:00

abstract：:Iron is a critical nutrient for the growth and survival of most bacterial species. Accordingly, much attention has been paid to the mechanisms by which host organisms sequester iron from invading bacteria and how bacteria acquire iron from their environment. However, under oxidative stress conditions such as those enc...

journal_title：Molecular microbiology

authors： Frawley ER,Fang FC

更新日期：2014-08-01 00:00:00

abstract：:CTXphi is a filamentous phage that encodes cholera toxin, one of the principal virulence factors of Vibrio cholerae. CTXphi is unusual among filamentous phages because it can either replicate as a plasmid or integrate into the V. cholerae chromosome at a specific site. The CTXphi genome has two regions, the 'core' and...

journal_title：Molecular microbiology

authors： Waldor MK,Rubin EJ,Pearson GD,Kimsey H,Mekalanos JJ

更新日期：1997-06-01 00:00:00

abstract：:Soupene et al. [J. Bacteriol. (2003) 185 5611-5626] made the unexpected observation that the presence of a mutation, in the gene for the N-acetylglucosamine repressor, nagC, increased the growth rate of Escherichia coli MG1655 on galactose, an unrelated sugar. We have found that NagC, binds to a single, high-affinity ...

journal_title：Molecular microbiology

authors： El Qaidi S,Allemand F,Oberto J,Plumbridge J

更新日期：2009-01-01 00:00:00

abstract：:We screened the putative rap-phr (response regulator aspartyl-phosphate phosphatase-phosphatase regulator) systems identified in the Bacillus subtilis genome for a rap gene that affects aprE (alkaline protease gene) expression by using a multicopy plasmid. We found that rapG was involved in the regulation of aprE, whi...

journal_title：Molecular microbiology

authors： Ogura M,Shimane K,Asai K,Ogasawara N,Tanaka T

更新日期：2003-09-01 00:00:00

abstract：:Beta-oxidation (beta-ox) occurs exclusively in the peroxisomes of Saccharomyces cerevisiae and other yeasts, leading to the supposition that fungi lack mitochondrial beta-ox. Here we present unequivocal evidence that the filamentous fungus Aspergillus nidulans houses both peroxisomal and mitochondrial beta-ox. While g...

journal_title：Molecular microbiology

authors： Maggio-Hall LA,Keller NP

更新日期：2004-12-01 00:00:00

abstract：:ICP is a chagasin-family natural tight binding inhibitor of Clan CA, family C1 cysteine peptidases (CPs). We investigated the role of ICP in Trypanosoma brucei by generating bloodstream form ICP-deficient mutants (Deltaicp). A threefold increase in CP activity was detected in lysates of Deltaicp, which was restored to...

journal_title：Molecular microbiology

authors： Santos CC,Coombs GH,Lima AP,Mottram JC

更新日期：2007-11-01 00:00:00

abstract：:The DnaA protein of Escherichia coli is a multi-functional protein which, In addition to promoting initiation of replication, can regulate the initiation or termination of transcription of a variety of genes. It acts by binding to DNA at a defined sequence, termed a DnaA-box. Three candidate DnaA-boxes which occur wit...

journal_title：Molecular microbiology

authors： Smith RW,McAteer S,Masters M

更新日期：1996-07-01 00:00:00

abstract：:Formation of the asymmetrically located septum divides sporulating bacilli into two distinct cells: the mother cell and the prespore. The rigidifying wall material in the septum is subsequently removed by autolysis. Examination of published electron micrographs indicates that the two septal membranes then fuse to form...

journal_title：Molecular microbiology

authors： Higgins ML,Piggot PJ

更新日期：1992-09-01 00:00:00

abstract：:Protection against maternal malaria has been associated with the acquisition of a specific antibody response that prevents adhesion of Plasmodium falciparum-infected erythrocytes to the glycosaminoglycan chondroitin-4-sulphate (CSA), which is present in the placental intervillous space. These antibodies are directed a...

journal_title：Molecular microbiology

authors： Andrews KT,Pirrit LA,Przyborski JM,Sanchez CP,Sterkers Y,Ricken S,Wickert H,Lépolard C,Avril M,Scherf A,Gysin J,Lanzer M

更新日期：2003-08-01 00:00:00

abstract：:The Crp/Fnr family of transcriptional regulators play central roles in transcriptional control of diverse physiological responses, and are activated by a surprising diversity of mechanisms. MrpC is a Crp/Fnr homolog that controls the Myxococcus xanthus developmental program. A long-standing model proposed that MrpC ac...

journal_title：Molecular microbiology

authors： Feeley BE,Bhardwaj V,McLaughlin PT,Diggs S,Blaha GM,Higgs PI

更新日期：2019-11-01 00:00:00

abstract：:Defensins represent an evolutionarily conserved group of small peptides with potent antibacterial activities. We report here that extracellular proteinases secreted by the human pathogens Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus pyogenes release dermatan sulphate by degrading dermatan sulphate-c...

journal_title：Molecular microbiology

authors： Schmidtchen A,Frick IM,Björck L

更新日期：2001-02-01 00:00:00

abstract：:Bacteria tightly regulate intracellular zinc levels to ensure sufficient zinc to support essential functions, while preventing toxicity. The bacterial response to zinc limitation includes the expression of putative zinc metallochaperones belonging to subfamily 1 of the COG0523 family of G3E GTPases. However, the clien...

journal_title：Molecular microbiology

authors： Chandrangsu P,Huang X,Gaballa A,Helmann JD

更新日期：2019-09-01 00:00:00

abstract：:The bacterial RNA-binding protein Hfq functions in post-transcriptional regulation of gene expression. There is evidence in a range of bacteria for specific subcellular localization of Hfq; however, the mechanism and role of Hfq localization remain unclear. Cyanobacteria harbour a subfamily of Hfq that is structurally...

journal_title：Molecular microbiology

authors： Schuergers N,Ruppert U,Watanabe S,Nürnberg DJ,Lochnit G,Dienst D,Mullineaux CW,Wilde A

更新日期：2014-05-01 00:00:00

abstract：:Most of the structural components of the flagellum of Salmonella typhimurium are exported through a flagellum-specific pathway, which is a member of the family of type III secretory pathways. The export apparatus for this process is poorly understood. A previous study has shown that two proteins, about 23 and 26 kDa i...

journal_title：Molecular microbiology

authors： Fan F,Ohnishi K,Francis NR,Macnab RM

更新日期：1997-12-01 00:00:00

abstract：:Protein translocation across the bacterial cytoplasmic membrane has been studied extensively in Escherichia coli. The identification of the components involved and subsequent reconstitution of the purified translocation reaction have defined the minimal constituents that allowed extensive biochemical characterization ...

journal_title：Molecular microbiology

authors： Manting EH,Driessen AJ

更新日期：2000-07-01 00:00:00

abstract：:SsrA is a tmRNA involved in tagging polypeptides on stalled ribosomes. The resulting fusion proteins are then degraded. We purified endogenous SsrA-tagged proteins by means of a genetically engineered SsrA and identified some of them. Analysis of the proteins suggested that they are tagged at their C-terminal extremit...

journal_title：Molecular microbiology

authors： Collier J,Binet E,Bouloc P

更新日期：2002-08-01 00:00:00

abstract：:MexAB-OprM is a multidrug efflux system that contributes to intrinsic and acquired multidrug resistance in Pseudomonas aeruginosa, the latter as a result of mutational hyperexpression of the mexAB-oprM operon. While efflux gene hyperexpression typically results from mutations in the linked mexR repressor gene, it also...

journal_title：Molecular microbiology

authors： Cao L,Srikumar R,Poole K

更新日期：2004-09-01 00:00:00