Abstract:
:A 4.0 kb EcoRI fragment of Bacillus subtilis conferring thiostrepton resistance was cloned and characterized. By nucleotide sequencing of the relevant region, six open reading frames were established, which corresponded to a part of spo0H, a ribosomal protein gene (rpmG), an unidentified open reading frame (orfE), a transcription antiterminator gene nusG, and ribosomal protein genes rplK and rplA. The orfE-encoded 59-amino-acid polypeptide had a low, but significant, sequence similarity with the carboxy-terminal region of the Escherichia coli SecE protein. A cold-sensitive secE mutation of E. coli was complemented by the plasmid-borne orfE sequence. Furthermore, the normal processing of a proOmpA protein was observed when the secE cold-sensitive strain carried an orfE plasmid, indicating that orfE is the secE homologue of B. subtilis. The B. subtilis secE has only one transmembrane sequence compared to the three in E. coli.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Jeong SM,Yoshikawa H,Takahashi Hdoi
10.1111/j.1365-2958.1993.tb00910.xsubject
Has Abstractpub_date
1993-10-01 00:00:00pages
133-42issue
1eissn
0950-382Xissn
1365-2958journal_volume
10pub_type
杂志文章abstract::CoiA is a transient protein expressed specifically during competence and required for genetic transformation in Streptococcus pneumoniae, but not for DNA uptake. It is widely conserved among Gram-positive bacteria but its function is unknown. Here we report that although the rate of DNA uptake was not affected in a co...
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