Heterochromatic marks are associated with the repression of secondary metabolism clusters in Aspergillus nidulans.

abstract：:Functional ribosomal RNAs are generated from longer precursor species in every organism known. Maturation of the 5' side of 16S rRNA in Escherichia coli is catalysed in a two-step process by the cooperative action of RNase E and RNase G. However, many bacteria lack RNase E and RNase G orthologues, raising the question...

journal_title：Molecular microbiology

authors： Britton RA,Wen T,Schaefer L,Pellegrini O,Uicker WC,Mathy N,Tobin C,Daou R,Szyk J,Condon C

更新日期：2007-01-01 00:00:00

abstract：:Reversible phase variation between the rugose and smooth colony variants is predicted to be important for the survival of Vibrio cholerae in natural aquatic habitats. Microarray expression profiling studies of the rugose and smooth variants of the same strain led to the identification of 124 differentially regulated g...

journal_title：Molecular microbiology

authors： Yildiz FH,Liu XS,Heydorn A,Schoolnik GK

更新日期：2004-07-01 00:00:00

abstract：:The DNA encoding the zinc metalloprotease of Legionella pneumophila Philadelphia 1 has been isolated and expressed in Escherichia coli. This protein, which is 38,000 Daltons in size, possesses immunological and biochemical properties identical to those previously described for the purified L. pneumophila protease. Per...

journal_title：Molecular microbiology

authors： Quinn FD,Tompkins LS

更新日期：1989-06-01 00:00:00

abstract：:Various features of the regulation of pathways for biodegradation of recalcitrant compounds by Pseudomonas provide insights into the mechanisms by which operons evolve to acquire conditionally active promoters that permit the corresponding genes to be transcribed only when required. The "regulatory noise hypothesis' p...

journal_title：Molecular microbiology

authors： de Lorenzo V,Pérez-Martín J

更新日期：1996-03-01 00:00:00

abstract：:Silent information regulator 2 (Sir2)-related proteins or sirtuins function as NAD(+)-dependent deacetylases or ADP ribosylases that target a range of substrates, thereby influencing chromatin structure and a diverse range of other biological functions. Genes encoding three Sir2-related proteins (SIR2rp1-3) have been ...

journal_title：Molecular microbiology

authors： Alsford S,Kawahara T,Isamah C,Horn D

更新日期：2007-02-01 00:00:00

abstract：:Natural sequence variation was investigated among serogroup A subgroup IV-1 Neisseria meningitidis isolated from diseased patients and healthy carriers in The Gambia, West Africa. The frequencies of DNA import were analysed by sequencing fragments of four linked genes encoding the immunogenic outer membrane proteins T...

journal_title：Molecular microbiology

authors： Linz B,Schenker M,Zhu P,Achtman M

更新日期：2000-06-01 00:00:00

abstract：:Small regulatory RNAs (sRNAs) are well known to command bacterial protein synthesis by modulating the translation and decay of target mRNAs. Most sRNAs are specifically regulated by a cognate transcription factor under certain growth or stress conditions. Investigations of the conserved Hfq-dependent MicM sRNA in Esch...

journal_title：Molecular microbiology

authors： Vogel J

更新日期：2009-09-01 00:00:00

abstract：:Accurate aminoacyl-tRNA synthesis is essential for correct translation of the genetic code in all organisms. Whereas many aspects of this process are conserved, others display a surprisingly high level of divergence from the canonical Escherichia coli model system. These differences are most pronounced in archaea wher...

journal_title：Molecular microbiology

authors： Praetorius-Ibba M,Ibba M

更新日期：2003-05-01 00:00:00

abstract：:Listeria monocytogenes FrvA (Lmo0641) is critical for virulence in the mouse model and is an ortholog of the Bacillus subtilis Fur- and PerR-regulated Fe(II) efflux P1B4 -type ATPase PfeT. Previously, FrvA was suggested to protect against heme toxicity. Here, we demonstrate that an frvA mutant is sensitive to iron int...

journal_title：Molecular microbiology

authors： Pi H,Patel SJ,Argüello JM,Helmann JD

更新日期：2016-06-01 00:00:00

abstract：:The cell walls of Sulfolobales species consist of proteinaceous S-layers assembled from two polypeptides, SlaA and SlaB. We isolated the large S-layer protein of Acidianus ambivalens and both S-layer subunits of Sulfolobus solfataricus and Metallosphaera sedula, respectively. The slaAB genes, lying adjacently in the c...

journal_title：Molecular microbiology

authors： Veith A,Klingl A,Zolghadr B,Lauber K,Mentele R,Lottspeich F,Rachel R,Albers SV,Kletzin A

更新日期：2009-07-01 00:00:00

abstract：:mscL encodes a channel in Escherichia coli that is opened by membrane stretch force, probably serving as an osmotic gauge. Sequences more or less similar to mscL are found in other bacteria, but the degree of conserved function has been unclear. We subcloned and expressed these putative homologues in E. coli and exami...

journal_title：Molecular microbiology

authors： Moe PC,Blount P,Kung C

更新日期：1998-05-01 00:00:00

abstract：:Certain endospore-forming soil dwelling bacteria are important human, animal or insect pathogens. These organisms produce spores containing an outer layer, the exosporium. The exosporium is the site of interactions between the spore and the soil environment and between the spore and the infected host during the initia...

journal_title：Molecular microbiology

authors： Stewart GC

更新日期：2017-05-01 00:00:00

abstract：UNLABELLED:Haem Nitric oxide/OXygen (H-NOX) binding domains are a family of haemoprotein sensors that are widespread in bacterial genomes, but limited information is available on their function. Legionella pneumophila is the only prokaryote found, thus far, to encode two H-NOX proteins. This paper presents data support...

journal_title：Molecular microbiology

authors： Carlson HK,Vance RE,Marletta MA

更新日期：2010-08-01 00:00:00

abstract：:We have isolated RNA polymerase from Mycobacterium smegmatis and established conditions for specific transcription initiation in vitro. The M. smegmatis enzyme has a strong dependence on supercoiling of the DNA substrate for transcription from mycobacterial promoters. We also show that RNA polymerase is the target for...

journal_title：Molecular microbiology

authors： Levin ME,Hatfull GF

更新日期：1993-04-01 00:00:00

abstract：:The porin oprE gene of Pseudomonas aeruginosa PAO1 was isolated. Its nucleotide sequence indicated that the structural gene of 1383 nucleotide residues encodes a precursor consisting of 460 amino acid residues with a signal peptide of 29 amino acid residues, which was confirmed by the N-terminal 23-amino-acid sequence...

journal_title：Molecular microbiology

authors： Yamano Y,Nishikawa T,Komatsu Y

更新日期：1993-05-01 00:00:00

abstract：:The phsA gene encodes phenoxazinone synthase (PHS), which catalyses the penultimate step in the pathway for actinomycin biosynthesis in Streptomyces antibioticus. The phsA promoter strikingly resembles a putative Streptomyces sigma E cognate promoter, and purified E sigma E holoenzyme transcribed the phsA promoter in ...

journal_title：Molecular microbiology

authors： Jones GH,Paget MS,Chamberlin L,Buttner MJ

更新日期：1997-01-01 00:00:00

abstract：:The Caulobacter crescentus flagellum serves not only as a motility apparatus, but also as a key landmark in the differentiation of this asymmetrically dividing bacterium. A distinctive aspect of flagellum biosynthesis is the periodic expression of the flagellar genes during the cell cycle in a sequence corresponding t...

journal_title：Molecular microbiology

authors： Wu J,Newton A

更新日期：1997-04-01 00:00:00

abstract：:The efficiency of conjugation of F-like plasmids is regulated by the FinOP fertility inhibition system. The transfer (tra) operon is under the direct control of the TraJ transcriptional activator which, in turn, is negatively regulated by FinP, an antisense RNA, and FinO, a 22 kDa protein. Recently, FinO has been show...

journal_title：Molecular microbiology

authors： van Biesen T,Söderbom F,Wagner EG,Frost LS

更新日期：1993-10-01 00:00:00

abstract：:Amino acid sequence analyses have indicated that the amino-terminal part of bacteriophage PRD1 structural protein P7 carries a conserved transglycosylase domain. We analysed wild-type PRD1 and different mutant particles in zymograms and found a glycolytic activity that was associated with protein P7. This is the first...

journal_title：Molecular microbiology

authors： Rydman PS,Bamford DH

更新日期：2000-07-01 00:00:00

abstract：:Polymorphonuclear neutrophils are the most important mammalian host defence cells against infections with Pseudomonas aeruginosa. Screening of a signature tagged mutagenesis library of the non-piliated P. aeruginosa strain TBCF10839 uncovered that transposon inactivation of its pilY1 gene rendered the bacterium more r...

journal_title：Molecular microbiology

authors： Bohn YS,Brandes G,Rakhimova E,Horatzek S,Salunkhe P,Munder A,van Barneveld A,Jordan D,Bredenbruch F,Häussler S,Riedel K,Eberl L,Jensen PØ,Bjarnsholt T,Moser C,Hoiby N,Tümmler B,Wiehlmann L

更新日期：2009-02-01 00:00:00

abstract：:In contrast to many type II restriction enzymes, dimeric proteins that cleave DNA at individual recognition sites 4-6 bp long, the SfiI endonuclease is a tetrameric protein that binds to two copies of an elongated sequence before cutting the DNA at both sites. The mode of action of the SfiI endonuclease thus seems mor...

journal_title：Molecular microbiology

authors： Bilcock DT,Halford SE

更新日期：1999-02-01 00:00:00

abstract：:Prior studies have shown that the catalase-deficient pathogen Streptococcus pyogenes (group A streptococcus) has a robust ability to resist oxidative stress that partially involves the transcriptional regulator PerR. However, the extent of the PerR regulon and the contribution of the members of this regulon to virulen...

journal_title：Molecular microbiology

authors： Brenot A,King KY,Caparon MG

更新日期：2005-01-01 00:00:00

abstract：:The cloned Citrobacter freundii ampC beta-lactamase is inducible in the presence of its regulatory gene ampR in Escherichia coli (Lindberg et al., 1985). The basal level of expression and inducibility are affected by two E. coli proteins encoded by the closely linked ampD and ampE genes. Deletion of both genes led to ...

journal_title：Molecular microbiology

authors： Lindquist S,Galleni M,Lindberg F,Normark S

更新日期：1989-08-01 00:00:00

abstract：:We have identified gene fusions of polyamine biosynthetic enzymes S-adenosylmethionine decarboxylase (AdoMetDC, speD) and aminopropyltransferase (speE) orthologues in diverse bacterial phyla. Both domains are functionally active and we demonstrate the novel de novo synthesis of the triamine spermidine from the diamine...

journal_title：Molecular microbiology

authors： Green R,Hanfrey CC,Elliott KA,McCloskey DE,Wang X,Kanugula S,Pegg AE,Michael AJ

更新日期：2011-08-01 00:00:00

abstract：:The K1 killer toxin of Saccharomyces cerevisiae consists of 103- and 83-residue alpha and beta components whose derivation, from a 316-residue precursor preprotoxin, requires processing at the alpha N-terminus (after ProArg-44), the alpha C-terminus (after ArgArg-149) and at the beta N-terminus (after LysArg-233). The...

journal_title：Molecular microbiology

authors： Zhu YS,Zhang XY,Cartwright CP,Tipper DJ

更新日期：1992-02-01 00:00:00

abstract：:Laboratory-based evolution and whole-genome sequencing can link genotype and phenotype. We used evolution of acid resistance in exponential phase Escherichia coli to study resistance to a lethal stress. Iterative selection at pH 2.5 generated five populations that were resistant to low pH in early exponential phase. G...

journal_title：Molecular microbiology

authors： Johnson MD,Bell J,Clarke K,Chandler R,Pathak P,Xia Y,Marshall RL,Weinstock GM,Loman NJ,Winn PJ,Lund PA

更新日期：2014-09-01 00:00:00

abstract：:Gram-negative bacteria have evolved numerous pathways to secrete proteins across their complex cell envelopes. Here, we describe a protein secretion system that uses a holin membrane protein in tandem with a cell wall-editing enzyme to mediate the secretion of substrate proteins from the periplasm to the cell exterior...

journal_title：Molecular microbiology

authors： Palmer T,Finney AJ,Saha CK,Atkinson GC,Sargent F

更新日期：2020-09-04 00:00:00

abstract：:Cryptococcus neoformans is a pathogenic yeast that causes life-threatening meningoencephalitis and grows well on mycological media regardless of inoculum size. Interestingly, a deletion of the global repressor TUP1 in C. neoformans uncovered a density-dependent growth phenotype reminiscent of the quorum-sensing phenom...

journal_title：Molecular microbiology

authors： Lee H,Chang YC,Nardone G,Kwon-Chung KJ

更新日期：2007-05-01 00:00:00

abstract：:Expression of the 987P gene cluster is activated by the adjacent IS1 element of an STpa transposon. Nucleotide sequence analysis of the 987P-DNA region contiguous with this IS1 element revealed the presence of an open reading frame designated fapR, encoding a basic protein of 260 amino acid residues with a molecular m...

journal_title：Molecular microbiology

authors： Klaasen P,de Graaf FK

更新日期：1990-10-01 00:00:00

abstract：:We have cloned a gene encoding a polygalacturonase (PG) in the filamentous fungus Aspergillus niger RH5344. The structural gene comprises 1141 bp coding for 362 amino acids and the open reading frame is disrupted by one intron of 52 bp. Eukaryotic consensus sequences for transcription regulation are found only in devi...

journal_title：Molecular microbiology

authors： Ruttkowski E,Khanh NQ,Wientjes FJ,Gottschalk M

更新日期：1991-06-01 00:00:00