Abstract:
:Advances in automated fluorescence microscopy have made snapshot and time-lapse imaging of bacterial cells commonplace, yet fundamental challenges remain in analysis. The vast quantity of data collected in high-throughput experiments requires a fast and reliable automated method to analyze fluorescence intensity and localization, cell morphology and proliferation as well as other descriptors. Inspired by effective yet tractable methods of population-level analysis using flow cytometry, we have developed a framework and tools for facilitating analogous analyses in image cytometry. These tools can both visualize and gate (generate subpopulations) more than 70 cell descriptors, including cell size, age and fluorescence. The method is well suited to multi-well imaging, analysis of bacterial cultures with high cell density (thousands of cells per frame) and complete cell cycle imaging. We give a brief description of the analysis of four distinct applications to emphasize the broad applicability of the tool.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Cass JA,Stylianidou S,Kuwada NJ,Traxler B,Wiggins PAdoi
10.1111/mmi.13591subject
Has Abstractpub_date
2017-03-01 00:00:00pages
818-828issue
5eissn
0950-382Xissn
1365-2958journal_volume
103pub_type
杂志文章abstract::The Escherichia coli urinary tract isolate C1212 contains two pyelonephritis-associated pili (pap) DNA sequences designated here as pap-17 and pap-21. Each of these pap sequences encodes antigenically-distinct pilin monomers, pilin-17 and pilin-21, respectively. Most individual strain C1212 cells isolated from a singl...
journal_title:Molecular microbiology
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doi:10.1111/j.1365-2958.1987.tb01940.x
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journal_title:Molecular microbiology
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pub_type: 杂志文章
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更新日期:2000-04-01 00:00:00
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journal_title:Molecular microbiology
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journal_title:Molecular microbiology
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:2006-09-01 00:00:00
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pub_type: 评论,杂志文章
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journal_title:Molecular microbiology
pub_type: 杂志文章
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journal_title:Molecular microbiology
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pub_type: 杂志文章
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更新日期:2007-07-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2002.03085.x
更新日期:2002-08-01 00:00:00
abstract::Plasmodium vivax is one of four Plasmodium species that cause human malaria. P. vivax and a related simian malaria parasite, Plasmodium knowlesi, invade erythrocytes by binding the Duffy antigen/receptor for chemokines (DARC) through their respective Duffy binding proteins. Here we show that tyrosines 30 and 41 of DAR...
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journal_title:Molecular microbiology
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更新日期:2001-03-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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journal_title:Molecular microbiology
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1995.mmi_18010045.x
更新日期:1995-10-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2014-08-01 00:00:00
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更新日期:2003-03-01 00:00:00