Abstract:
:Cell-cell and cell-surface adherence represents initial steps in forming multicellular aggregates or in establishing cell-surface interactions. The commonly used Saccharomyces cerevisiae laboratory strain S288c carries a flo8 mutation, and is only able to express the flocculin-encoding genes FLO1 and FLO11, when FLO8 is restored. We show here that the two flocculin genes exhibit differences in regulation to execute distinct functions under various environmental conditions. In contrast to the laboratory strain Sigma1278b, haploids of the S288c genetic background require FLO1 for cell-cell and cell-substrate adhesion, whereas FLO11 is required for pseudohyphae formation of diploids. In contrast to FLO11, FLO1 repression requires the Sin4p mediator tail component, but is independent of the repressor Sfl1p. FLO1 regulation also differs from FLO11, because it requires neither the KSS1 MAP kinase cascade nor the pathways which lead to the transcription factors Gcn4p or Msn1p. The protein kinase A pathway and the transcription factors Flo8p and Mss11p are the major regulators for FLO1 expression. Therefore, S. cerevisiae is prepared to simultaneously express two genes of its otherwise silenced FLO reservoir resulting in an appropriate cellular surface for different environments.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Fichtner L,Schulze F,Braus GHdoi
10.1111/j.1365-2958.2007.06014.xsubject
Has Abstractpub_date
2007-12-01 00:00:00pages
1276-89issue
5eissn
0950-382Xissn
1365-2958pii
MMI6014journal_volume
66pub_type
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journal_title:Molecular microbiology
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pub_type: 杂志文章,评审
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pub_type: 杂志文章,评审
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