Abstract:
:The genes encoding the three terminal enzymes in the threonine biosynthetic pathway, homoserine dehydrogenase (hom), homoserine kinase (thrB) and threonine synthase (thrC) have been isolated from Corynebacterium glutamicum. The C. glutamicum hom and thrB genes were subcloned on a 3.6 kb SalI-generated chromosomal fragment. The C. glutamicum thrC gene was shown not to be linked to the hom-thrB locus. L-methionine represses the cloned homoserine dehydrogenase and homoserine kinase similar to that of the chromosomally encoded hom and thrB gene products. Northern hybridization analysis demonstrates that this repression is mediated at the level of transcription and that hom-thrB represents an operon in C. glutamicum.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Follettie MT,Shin HK,Sinskey AJdoi
10.1111/j.1365-2958.1988.tb00006.xsubject
Has Abstractpub_date
1988-01-01 00:00:00pages
53-62issue
1eissn
0950-382Xissn
1365-2958journal_volume
2pub_type
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