Abstract:
:The porins of Gram-negative bacteria are responsible for the 'molecular sieve' properties of the outer membrane. They form large water-filled channels which allow the diffusion of hydrophilic molecules into the periplasmic space. Owing to the strong hydrophilicity of their amino acid sequence and the nature of their secondary structure (beta strands), conventional hydropathy methods for predicting membrane topology are useless for this class of protein. The large number of available porin amino acid sequences was exploited to improve the accuracy of the prediction in combination with tools detecting amphipathicity of secondary structure. Using the constraints of beta-sheet structure these porins are predicted to contain 16 membrane-spanning strands, 14 of which are common to the two (enteric and the neisserial) porin subfamilies.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Jeanteur D,Lakey JH,Pattus Fdoi
10.1111/j.1365-2958.1991.tb02145.xsubject
Has Abstractpub_date
1991-09-01 00:00:00pages
2153-64issue
9eissn
0950-382Xissn
1365-2958journal_volume
5pub_type
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