Abstract：
:A method called Muprinting has been developed that uses PCR to generate a detailed picture of the bacteriophage Mu transposition sites in chosen domains of the bacterial chromosome. Muprinting experiments in Escherichia coli show that the frequency of phage integration changes dramatically near two repressor binding sites in the lac operon. When the lac operon was repressed, hotspots for Mu transposition were found near the O1 and O2 operators that are proposed to make a repression loop. When cells were grown in lactose, Mu transposition near these operators was greatly diminished. Striking changes in transposition frequencies were limited to the control region and were not found in a region of the lacZ gene lying beyond the O2 operator. Muprints of the bgl operon showed a different pattern; hotspots for Mu transposition detected in sequences upstream of the bglC promoter when the operon was silenced changed when the operon became activated by mutation. By targeting transposition to the regulatory regions around non-expressed genes, Mu may demonstrate a self-restraint mechanism that allows the virus to move through its host genome without disrupting the functions that contribute to a healthy cell physiology.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Wang X,Higgins NPdoi
10.1111/j.1365-2958.1994.tb01054.xsubject
Has Abstractpub_date
1994-05-01 00:00:00pages
665-77issue
4eissn
0950-382Xissn
1365-2958journal_volume
12pub_type
杂志文章abstract：:Engulfment in Bacillus subtilis is mediated by two complementary systems, SpoIID, SpoIIM and SpoIIP (DMP), which are essential for engulfment, and the SpoIIQ-SpoIIIAGH (Q-AH) zipper, which provides a secondary engulfment mechanism and recruits other proteins to the septum. We here identify two mechanisms by which DMP ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2007.05887.x
更新日期：2007-09-01 00:00:00
abstract：:A virulence-associated region in the genome of Dichelobacter nodosus has been shown to contain an integrase gene which is highly related to the integrases of Shigella flexneri phage Sf6 and coliphages P4 and phi R73, together with open reading frames (vapB, C and D) related to genes borne on plasmids in Neisseria gono...
journal_title：Molecular microbiology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2958.1995.mmi_18020201.x
更新日期：1995-10-01 00:00:00
abstract：:Elevated expression of the plasma membrane drug efflux pump proteins Cdr1p and Cdr2p was shown to accompany decreased azole susceptibility in Candida albicans clinical isolates. DNA sequence analysis revealed extensive allelic heterozygosity, particularly of CDR2. Cdr2p alleles showed different abilities to transport ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2006.05357.x
更新日期：2006-10-01 00:00:00
abstract：:On the basis of the published N-terminal amino acid sequence of the soluble lytic transglycosylase 35 (Slt35) of Escherichia coli, an open reading frame (ORF) was cloned from the 60.8 min region of the E. coli chromosome. The nucleotide sequence of the ORF, containing a putative lipoprotein-processing site, was shown ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1995.tb02437.x
更新日期：1995-05-01 00:00:00
abstract：:SigD and SigE (Salmonella invasion gene) are proteins needed for optimal invasion of Salmonella typhimurium into eukaryotic cells in vitro. SigD is a secreted protein and SigE is a putative chaperone required for SigD stability and/or secretion. SigD is secreted by a type III secretion apparatus encoded within a patho...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2000.01772.x
更新日期：2000-02-01 00:00:00
abstract：:Accumulation of stalled ribosomes at the 3' end of mRNA without a stop codon (non-stop mRNA) is supposed to be toxic to bacterial cells. Escherichia coli has at least two distinct systems to rescue such stalled ribosomes: SsrA-dependent trans-translation and ArfA-dependent ribosome rescue. Combination of the ssrA and ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2011.07607.x
更新日期：2011-05-01 00:00:00
abstract：:The nucleotide sequence of the proximal half of the rfb region of Shigella flexneri has been determined, and the genes encoding enzymes involved in the biosynthesis of dTDP-rhamnose have been identified. These genes show strong homology to the rfb genes encoding dTDP-rhamnose biosynthesis in Salmonella enterica serova...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1994.tb00308.x
更新日期：1994-01-01 00:00:00
abstract：:The ferric uptake regulator (Fur) protein is known to act as a Fe2+-dependent transcriptional repressor of bacterial promoters. Here, we show that, in Helicobacter pylori, Fur can mediate the regulation of iron-activated genes in contrast to classical Fur regulation, in which iron acts as a co-repressor. Inactivation ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2001.02696.x
更新日期：2001-12-01 00:00:00
abstract：:The dominant, polA1-independent replicon of pGSH500, rep beta (1.8 kb), consists of a cis-acting oriV region of 245 bp; a repB gene that is essential for autonomous replication and 18, 30 to 36 bp iterons which constitute the inc/cop region. The molecular organization of rep beta resembles that of mini-pCU1 (IncN). Fu...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1993.tb01171.x
更新日期：1993-03-01 00:00:00
abstract：:Silent information regulator 2 (Sir2)-related proteins or sirtuins function as NAD(+)-dependent deacetylases or ADP ribosylases that target a range of substrates, thereby influencing chromatin structure and a diverse range of other biological functions. Genes encoding three Sir2-related proteins (SIR2rp1-3) have been ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2006.05553.x
更新日期：2007-02-01 00:00:00
abstract：:Legionella pneumophila can replicate inside amoebae and also alveolar macrophages to cause Legionnaires' Disease in susceptible hosts. When nutrients become limiting, a stringent-like response coordinates the differentiation of L. pneumophila to a transmissive form, a process mediated by the two-component system LetA/...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2003.03706.x
更新日期：2003-10-01 00:00:00
abstract：:Several Fusarium species produce the polyketide mycotoxin zearalenone (ZEA), a causative agent of hyperestrogenic syndrome in animals that is often found in F. graminearum-infected cereals in temperate regions. The ZEA biosynthetic cluster genes PKS4, PKS13, ZEB1 and ZEB2 encode a reducing polyketide synthase, a non-r...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.13078
更新日期：2015-09-01 00:00:00
abstract：:Overexpression of the MDR1 gene, encoding a multi-drug efflux pump of the major facilitator superfamily, is a major cause of resistance to the widely used antifungal agent fluconazole and other toxic substances in the fungal pathogen Candida albicans. We found that all tested clinical and in vitro generated C. albican...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2008.06309.x
更新日期：2008-08-01 00:00:00
abstract：:In vivo and in vitro analyses indicate that transcription of the argO gene coding for an arginine exporter is regulated by the global transcriptional regulator Lrp, an effect that went by unnoticed in previous genome-scale screenings of the Lrp regulatory network in Escherichia coli. Lrp activates the argO promoter fo...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2009.06950.x
更新日期：2009-12-01 00:00:00
abstract：:The NAC (nitrogen assimilation control) protein from Klebsiella aerogenes is a LysR-like regulator for transcription of several operons involved in nitrogen metabolism, and couples the transcription of these sigma 70-dependent operons to regulation by the sigma 54-dependent NTR system. NAC activates expression of oper...
journal_title：Molecular microbiology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2958.1991.tb01965.x
更新日期：1991-11-01 00:00:00
abstract：:The chromosome ends of human malaria parasites harbour many genes encoding proteins that are exported to the surface of infected red cells, often being involved in host-parasite interactions and immune evasion. Unlike other murine malaria parasites Plasmodium chabaudi undergoes antigenic variation during passage in th...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2003.03491.x
更新日期：2003-06-01 00:00:00
abstract：:Prior studies have shown that the catalase-deficient pathogen Streptococcus pyogenes (group A streptococcus) has a robust ability to resist oxidative stress that partially involves the transcriptional regulator PerR. However, the extent of the PerR regulon and the contribution of the members of this regulon to virulen...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2004.04370.x
更新日期：2005-01-01 00:00:00
abstract：:The four motor proteins PomA, PomB, MotX and MotY, which are believed to be stator proteins, are essential for motility by the Na(+)-driven flagella of Vibrio alginolyticus. When we purified the flagellar basal bodies, MotX and MotY were detected in the basal body, which is the supramolecular complex comprised of the ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2006.05435.x
更新日期：2006-11-01 00:00:00
abstract：:We have determined the sizes of the chromosomes of six Bacillus cereus strains (range 2.4-4.3 Mb) and constructed a physical map of the smallest B. cereus chromosome (2.4 Mb). This map was compared to those of the chromosomes of four B. cereus strains and one B. thuringiensis strain previously determined to be 5.4-6.3...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1994.tb00411.x
更新日期：1994-07-01 00:00:00
abstract：:We report the characterization of the msbA gene, isolated as a multicopy suppressor of the HtrB temperature-sensitive phenotype. The msbA gene maps to 20.5 min on the Escherichia coli genetic map and encodes a protein with an estimated molecular mass of 64,460 Da, with the properties of an integral membrane protein. T...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1993.tb01098.x
更新日期：1993-01-01 00:00:00
abstract：:Certain strains of Pichia acaciae and Wingea robertsiae (synonym Debaryomyces robertsiae) harbour extranuclear genetic elements that confer a killer phenotype to their host. Such killer plasmids (pPac1-2 of P. acaciae and pWR1A of W. robertsiae) were sequenced and compared with the zymocin encoding pGKL1 of Kluyveromy...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2004.04119.x
更新日期：2004-07-01 00:00:00
abstract：:We present structural and biochemical evidence for a redox switch in the archaeal transcriptional regulator SurR of Pyrococcus furiosus, a hyperthermophilic anaerobe. P. furiosus produces H(2) during fermentation, but undergoes a metabolic shift to produce H(2) S when elemental sulfur (S(0) ) becomes available. Change...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2010.07275.x
更新日期：2010-09-01 00:00:00
abstract：:When confronted with a stress factor, bacteria react with a specific stress response, a genetically encoded programme resulting in the transiently enhanced expression of a subset of genes. One of these stress factors is a sudden increase in the external pH. As a first step to understand the response of Bacillus subtil...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2001.02489.x
更新日期：2001-07-01 00:00:00
abstract：:The chromosomal DpnII gene cassette of Streptococcus pneumoniae encodes two methyltransferases and an endonuclease. One methyltransferase acts on double-stranded and the other on single-stranded DNA. Two mRNAs are transcribed from the cassette. One, a SigA promoter transcript, includes all three genes; the other inclu...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2000.01777.x
更新日期：2000-03-01 00:00:00
abstract：:A series of lipoproteins was detected in the membrane fraction of Yersinia enterocolitica W227, a typical strain from serotype O:9. At least two of them, YlpA and YlpB, are encoded by the pYV plasmid. The sequence of ylpA reveals the presence of a typical lipoprotein signal peptide. The mature YlpA protein would be 22...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1990.tb02070.x
更新日期：1990-09-01 00:00:00
abstract：:In this work, we have studied the in vitro translational features of a bicistronic mRNA of the extremely thermophilic Archaeon Sulfolobus solfataricus, with the aim of determining the nature of the cis-acting signals controlling the recognition of the translation initiation sites in the Archaea. We found that the most...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.1999.01615.x
更新日期：1999-10-01 00:00:00
abstract：:The Escherichia coli fructose repressor, FruR, is known to regulate expression of several genes concerned with carbon utilization. Using a previously derived consensus sequence for FruR binding, additional potential operators were identified and tested for FruR binding in DNA band migration retardation assays. Operato...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1995.tb02339.x
更新日期：1995-06-01 00:00:00
abstract：:Recent progress in the analysis of mutants of the Escherichia coli lactose carrier function is reviewed, with special emphasis on the structural basis for energy barriers which prevent 'forbidden' conformational changes. Mutations which break down the barriers to forbidden isomerizations involving the binary carrier:s...
journal_title：Molecular microbiology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2958.1990.tb02053.x
更新日期：1990-09-01 00:00:00
abstract：:Clp ATPases, which include the ubiquitous HSP100 family, are classified according to their structural features and sequence similarities. During the course of the Bacillus subtilis genome sequencing project, we identified a gene encoding a new member of the HSP100 family. We designated this protein ClpE, as it is the ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.1999.01374.x
更新日期：1999-05-01 00:00:00
abstract：:A number of plasmid-encoded gene systems are thought to stabilize plasmids by killing plasmid-free cells (also termed post-segregational killing or plasmid addiction). Here we analyse the mechanisms of plasmid stabilization by ccd of F, parDE of RP4 and parD of R1, and compare them to hok/sok of R1. To induce synchron...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1995.mmi_17020211.x
更新日期：1995-07-01 00:00:00
