Abstract:
:Cell wall lipids of Mycobacterium tuberculosis containing multiple methylbranched fatty acids play critical roles in pathogenesis and thus offer targets for new antimycobacterial drugs. Mycocerosicacid synthase gene (mas) encodes the enzyme that produces one class of such acids. Seven mas-like genes (msls) were identified in the genome. One of them, msl3, originally annotated as two separate genes, pks 3 and pks 4, is now shown to constitute a single open reading frame, which encodes a 220.3 kDa protein. Msl3 was disrupted using a phage mediated delivery system and the gene replacement in the mutant was confirmed by polymerase chain reaction analysis of the flanking regions of the introduced disrupted gene and by Southern analysis. Biochemical analysis showed that the msl3 mutant does not produce mycolipanoic acids and mycolipenic(phthienoic) acids, the major constituents of polyacyl trehaloses and thus lacks this cell wall lipid, but synthesizes all of the other classes of lipids. The absence of the major acyl chains that anchor the surface-exposed acyltrehaloses causes a novel growth morphology; the cells stick to each other, most probably via the intercellular interaction between the exposed hydrophobic cell surfaces, manifesting a bead-like growth morphology without affecting the overall growth rate.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Dubey VS,Sirakova TD,Kolattukudy PEdoi
10.1046/j.1365-2958.2002.03119.xsubject
Has Abstractpub_date
2002-09-01 00:00:00pages
1451-9issue
5eissn
0950-382Xissn
1365-2958pii
3119journal_volume
45pub_type
杂志文章abstract::Transcription of lysine genes in Saccharomyces cerevisiae is dependent on Lys14p and on alpha-aminoadipate semialdehyde (alphaAASA), an intermediate of the pathway. The two-thirds C-terminal end of Lys14p is sufficient to ensure the activation function of the protein and its modulation by alphaAASA. Here, we show that...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2002.02854.x
更新日期:2002-03-01 00:00:00
abstract::A segment of the exotoxin A gene of Pseudomonas aeruginosa, coding for the N-terminal end of domain I and domain II of the toxin (ETA), was genetically fused to the diphtheria toxin gene of Corynebacterium diphtheriae, coding for the N-terminal end of A fragment of diphtheria toxin (DT). The resulting hybrid protein (...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1992.tb00849.x
更新日期:1992-05-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1996.363898.x
更新日期:1996-01-01 00:00:00
abstract::The Gram-positive pathogen Corynebacterium diphtheriae exports through the Sec apparatus many extracellular proteins that include the key virulence factors diphtheria toxin and the adhesive pili. How these proteins attain their native conformations after translocation as unfolded precursors remains elusive. The fact t...
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pub_type: 杂志文章
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更新日期:2015-12-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2003.03596.x
更新日期:2003-08-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.12337
更新日期:2013-09-01 00:00:00
abstract::The exosporium is the outermost layer of the Bacillus anthracis spore. The predominant protein on the exosporium surface is BclA, a collagen-like glycoprotein. BclA is incorporated on the spore surface late in the B. anthracis sporulation pathway. A second collagen-like protein, BclB, has been shown to be surface-expo...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2008.06420.x
更新日期:2008-10-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 评论
doi:10.1111/mmi.13651
更新日期:2017-05-01 00:00:00
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更新日期:1995-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1365-2958.2011.07854.x
更新日期:2011-11-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1999.01266.x
更新日期:1999-02-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2001.02493.x
更新日期:2001-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/mmi.12599
更新日期:2014-06-01 00:00:00
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pub_type: 杂志文章,收录出版
doi:10.1111/j.1365-2958.2007.05928.x
更新日期:2007-10-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2012.07994.x
更新日期:2012-05-01 00:00:00
abstract::Encased within the 280 kb genome in the capsid of the giant myovirus φKZ is an unusual cylindrical proteinaceous 'inner body' of highly ordered structure. We present here mass spectrometry, bioinformatic and biochemical studies that reveal novel information about the φKZ head and the complex inner body. The identifica...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2012.08025.x
更新日期:2012-04-01 00:00:00
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pub_type: 评论,杂志文章
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更新日期:2014-10-01 00:00:00
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更新日期:2014-10-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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journal_title:Molecular microbiology
pub_type: 杂志文章,评审
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更新日期:1995-05-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1995.tb02263.x
更新日期:1995-02-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2010-04-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1999-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-10-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1996-02-01 00:00:00