Abstract：
:In this study we show for the first time the intracellular distribution of a K39 kinesin homologue in Leishmania donovani, a medically important parasite of humans. Further, we demonstrated that this motor protein is expressed in both the insect and mammalian developmental forms (i.e. promastigote and amastigotes) of this organism. Moreover, in both of these parasite developmental stages, immunofluorescence indicated that the LdK39 kinesin accumulated at anterior and posterior cell poles and that it displayed a peripheral localization consistent with the cortical cytoskeleton. Using a molecular approach, we identified, cloned and characterized the first complete open reading frame for the gene (LdK39) encoding this large (> 358 kDa) motor protein in L. donovani. Based on these observations, we subsequently used a homologous episomal expression system to dissect and express the functional domains that constitute the native molecule. Cell fractionation experiments demonstrated that LdK39 was soluble and that it bound to detergent-extracted cytoskeletons of these parasites in an ATP-dependent manner. The cumulative results of these experiments are consistent with LdK39 functioning as an ATP-dependent kinesin which binds to and travels along the cortical cytoskeleton of this important human pathogen.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Gerald NJ,Coppens I,Dwyer DMdoi
10.1111/j.1365-2958.2006.05487.xsubject
Has Abstractpub_date
2007-02-01 00:00:00pages
962-79issue
4eissn
0950-382Xissn
1365-2958pii
MMI5487journal_volume
63pub_type
杂志文章abstract：:A 25 kbp DNA fragment from the chromosome of Rhodobacter capsulatus B10 carrying hydrogenase (hup) determinants was completely sequenced. Coding regions corresponding to 20 open reading frames were identified. The R. capsulatus hydrogenase-specific gene (hup and hyp) products bear significant structural identity to hy...
journal_title：Molecular microbiology
pub_type： 杂志文章
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journal_title：Molecular microbiology
pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：1994-05-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
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journal_title：Molecular microbiology
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journal_title：Molecular microbiology
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