The DNA binding protein Tfx from Methanobacterium thermoautotrophicum: structure, DNA binding properties and transcriptional regulation.

abstract：:The 29 kDa protein of pathogenic Entamoeba histolytica is a cysteine-rich surface antigen which we recently characterized by cDNA sequencing and by using monoclonal antibodies which differentiated between pathogenic and non-pathogenic clinical isolates. To determine the structure and biochemical attributes of this pro...

journal_title：Molecular microbiology

authors： Flores BM,Batzer MA,Stein MA,Petersen C,Diedrich DL,Torian BE

更新日期：1993-03-01 00:00:00

abstract：:Type III secretion systems (T3SSs) are protein injection devices essential for the interaction of many Gram-negative bacteria with eukaryotic cells. While Shigella assembles its T3SS when the environmental conditions are appropriate for invasion, secretion is only activated after physical contact with a host cell. Fir...

journal_title：Molecular microbiology

authors： Roehrich AD,Guillossou E,Blocker AJ,Martinez-Argudo I

更新日期：2013-02-01 00:00:00

abstract：:The Frz pathway of Myxococcus xanthus controls cell reversal frequency to support directional motility during swarming and fruiting body formation. Previously, we showed that phosphorylation of the response regulator FrzZ correlates with reversal frequencies, suggesting that this activity represents the output of the ...

journal_title：Molecular microbiology

authors： Kaimer C,Zusman DR

更新日期：2016-04-01 00:00:00

abstract：:Toxin-antitoxin loci have been identified in almost all free-living prokaryotes, often in high copy numbers. The biological function and molecular targets of the abundant vapBC loci are not yet known. Here we analyse the vapBC loci of Salmonella LT2 and Shigella plasmid pMYSH6000. Both loci encode putative PIN (PilT N...

journal_title：Molecular microbiology

authors： Winther KS,Gerdes K

更新日期：2009-05-01 00:00:00

abstract：:When confronted with a stress factor, bacteria react with a specific stress response, a genetically encoded programme resulting in the transiently enhanced expression of a subset of genes. One of these stress factors is a sudden increase in the external pH. As a first step to understand the response of Bacillus subtil...

journal_title：Molecular microbiology

authors： Wiegert T,Homuth G,Versteeg S,Schumann W

更新日期：2001-07-01 00:00:00

abstract：:PII-like signalling molecules are trimeric proteins composed of 12-13 kDa polypeptides encoded by the glnB gene family. Heterologous expression of a cyanobacterial glnB gene in Escherichia coli leads to an inactivation of E. coli's own PII signalling system. In the present work, we show that this effect is caused by t...

journal_title：Molecular microbiology

authors： Forchhammer K,Hedler A,Strobel H,Weiss V

更新日期：1999-07-01 00:00:00

abstract：:Previous studies with purified variants of the 434 repressor having different operator-binding specificities have demonstrated the interactions of a heterodimeric repressor with a hybrid operator site. The present study investigates the interactions between the 434 repressor and its operator site. The optimum 434 oper...

journal_title：Molecular microbiology

authors： Webster C,Merryweather A,Brammar W

更新日期：1992-02-01 00:00:00

abstract：:Fungal pathogens have evolved sophisticated machinery to precisely balance the fine line between acquiring essential metals and defending against metal toxicity. Iron and copper are essential metals for many processes in both fungal pathogens and their mammalian hosts, but reduce viability when present in excess. Howe...

journal_title：Molecular microbiology

authors： Ding C,Festa RA,Sun TS,Wang ZY

更新日期：2014-07-01 00:00:00

abstract：:The lipoprotein Lpp is the most numerically abundant protein in Escherichia coli, has been investigated for over 40 years, and has served as the paradigmatic bacterial lipoprotein since its initial discovery. It exists in two distinct forms: a 'bound-form', which is covalently bound to the cell's peptidoglycan layer, ...

journal_title：Molecular microbiology

authors： Cowles CE,Li Y,Semmelhack MF,Cristea IM,Silhavy TJ

更新日期：2011-03-01 00:00:00

abstract：:Bordetella bronchiseptica is a pathogen that can acquire iron using its native alcaligin siderophore system, but can also use the catechol xenosiderophore enterobactin via the BfeA outer membrane receptor. Transcription of bfeA is positively controlled by a regulator that requires induction by enterobactin. Catecholam...

journal_title：Molecular microbiology

authors： Armstrong SK,Brickman TJ,Suhadolc RJ

更新日期：2012-05-01 00:00:00

abstract：:Factors that enhance the transmission of pathogens are poorly understood. We show that Vibrio cholerae shed in human 'rice-water' stools have a 10-fold lower oral infectious dose in an animal model than in vitro grown V. cholerae, which may aid in transmission during outbreaks. Furthermore, we identify a bacterial fac...

journal_title：Molecular microbiology

authors： Butler SM,Nelson EJ,Chowdhury N,Faruque SM,Calderwood SB,Camilli A

更新日期：2006-04-01 00:00:00

abstract：:The best studied role of ubiquitination is to mark proteins for destruction by the proteasome but, in addition, it has recently been shown to promote macromolecular assembly and function, and alter protein function, thus playing a regulatory role distinct from protein degradation. Deubiquinating enzymes, the ubiquitin...

journal_title：Molecular microbiology

authors： Lockington RA,Kelly JM

更新日期：2001-06-01 00:00:00

abstract：:A growing class of proteins regulates transcription through interaction with DNA-dependent RNA polymerase. Here we report that a recently identified, highly conserved sporulation gene ylyA encodes a novel RNA polymerase-binding protein that influences the expression of genes under the control of the late-acting, sporu...

journal_title：Molecular microbiology

authors： Traag BA,Ramirez-Peralta A,Wang Erickson AF,Setlow P,Losick R

更新日期：2013-07-01 00:00:00

abstract：:A number of strains of the widespread aerobic soil bacterium, Bacillus sphaericus, possess crystalline inclusions of a toxin lethal to a variety of insect (larvae) which are vectors of major tropical diseases. Partial amino acid sequence data from one strain, B. sphaericus 2362 have permitted us to design oligonucleot...

journal_title：Molecular microbiology

authors： Hindley J,Berry C

更新日期：1987-09-01 00:00:00

abstract：:We have characterized a number of mutations in fnr that interfere with FNR-dependent transcription activation at two promoters where the FNR-binding site is centred around 41 1/2 bp upstream from the transcription start site. The substituted residues in all but one of these FNR mutants are clustered around a presumed ...

journal_title：Molecular microbiology

authors： Bell A,Busby S

更新日期：1994-01-01 00:00:00

abstract：:Ustilago maydis is able to initiate pathogenic development after fusion of two haploid cells with different mating type. On the maize leaf surface, the resulting dikaryon switches to filamentous growth, differentiates appressoria and penetrates the host. Here, we report on the plant signals required for filament forma...

journal_title：Molecular microbiology

authors： Mendoza-Mendoza A,Berndt P,Djamei A,Weise C,Linne U,Marahiel M,Vranes M,Kämper J,Kahmann R

更新日期：2009-02-01 00:00:00

abstract：:AtzR is a LysR-type regulator responsible for activation of the cyanuric acid utilization operon atzDEF. AtzR binds the PatzDEF promoter region at a strong recognition element, designated the repressor binding site, and a weaker binding determinant, the activator binding site (ABS). AtzR activates transcription in res...

journal_title：Molecular microbiology

authors： Porrúa O,Platero AI,Santero E,Del Solar G,Govantes F

更新日期：2010-04-01 00:00:00

abstract：:Bordetella bronchiseptica is a common respiratory tract pathogen of many mammalian species. Nucleotide sequences from the locus involved in coordinate regulation of B. pertussis virulence factors, vir, were shown to have a high degree of homology to chromosomal DNA from virulent (Vir+) and avirulent (Vir-) strains of ...

journal_title：Molecular microbiology

authors： Monack DM,Arico B,Rappuoli R,Falkow S

更新日期：1989-12-01 00:00:00

abstract：:Bordetella pertussis regulates expression of its virulence factors such as pertussis toxin (Ptx) via the bvg locus, which encodes a two-component system composed of a sensor protein, BvgS, and a transcription activator, BvgA. We used a ptx-lac fusion on the B. pertussis chromosome to analyse promoter activation by alt...

journal_title：Molecular microbiology

authors： Marques RR,Carbonetti NH

更新日期：1997-06-01 00:00:00

abstract：:In response to discrete environmental cues, Saccharomyces cerevisiae cells adjust patterns of gene expression and protein activity to optimize metabolism. Nutrient-sensing systems situated in the plasma membrane (PM) of yeast have only recently been discovered. Ssy1p is one of three identified components of the Ssy1p-...

journal_title：Molecular microbiology

authors： Forsberg H,Gilstring CF,Zargari A,Martínez P,Ljungdahl PO

更新日期：2001-10-01 00:00:00

abstract：:Tst, the gene for toxic shock syndrome toxin-1 (TSST-1), is part of a 15.2 kb genetic element in Staphylococcus aureus that is absent in TSST-1-negative strains. The prototype, in RN4282, is flanked by a 17 nucleotide direct repeat and contains genes for a second possible superantigen toxin, a Dichelobacter nodosus Va...

journal_title：Molecular microbiology

authors： Lindsay JA,Ruzin A,Ross HF,Kurepina N,Novick RP

更新日期：1998-07-01 00:00:00

abstract：:Genetic recombination impacts on neisserial biology in two ways: (i) specific loci undergo rearrangement at high frequency leading to the formation of many different alleles; and (ii) Neisseria, being naturally competent for DNA transformation, provide a means to disseminate the novel alleles throughout a population. ...

journal_title：Molecular microbiology

authors： Hill SA

更新日期：1996-05-01 00:00:00

abstract：:Bacterial autotransporters consist of an N-terminal 'passenger domain' that is transported into the extracellular space by an unknown mechanism and a C-terminal 'beta-domain' that forms a beta-barrel in the outer membrane. Recent studies have revealed that fully assembled autotransporters have an unusual architecture ...

journal_title：Molecular microbiology

authors： Ieva R,Skillman KM,Bernstein HD

更新日期：2008-01-01 00:00:00

abstract：:The stable maintenance of low-copy-number plasmids requires active partitioning, with the most common mechanism in prokaryotes involving the ATPase ParA. ParA proteins undergo intricate spatiotemporal relocations across the nucleoid, dynamics that function to position plasmids at equally spaced intervals. This spacing...

journal_title：Molecular microbiology

authors： Howard M,Gerdes K

更新日期：2010-10-01 00:00:00

abstract：:Choline-binding proteins (CBPs) from Streptococcus pneumoniae are involved in several important processes. Inactivation of zmpB, a gene that encodes a surface-located putative zinc metalloprotease, in a S. pneumoniae serotype 4 strain was recently reported to reveal a composite phenotype, including extensive chain for...

journal_title：Molecular microbiology

authors： Bergé M,García P,Iannelli F,Prère MF,Granadel C,Polissi A,Claverys JP

更新日期：2001-03-01 00:00:00

abstract：:The DNA repeat region of fcrA76, the gene encoding a group A streptococcal Fc-binding protein, was subcloned in-frame into an Escherichia coli plasmid expression vector. The expressed protein product displayed the same Fc-binding properties as the full-length Fc-binding protein expressed from fcrA76. The affinity-puri...

journal_title：Molecular microbiology

authors： Heath DG,Boyle MD,Cleary PP

更新日期：1990-12-01 00:00:00

abstract：:Proteinaceous, hair-like appendages known as fimbriae or pili commonly extend from the surface of prokaryotic cells and serve important functions such as cell adhesion, biofilm formation, motility and DNA transfer. Here we show that a novel group of archaea from cold, sulphidic springs has developed cell surface appen...

journal_title：Molecular microbiology

authors： Moissl C,Rachel R,Briegel A,Engelhardt H,Huber R

更新日期：2005-04-01 00:00:00

abstract：:Bacteria sense and respond to their environment through the use of two-component regulatory systems. The ability to adapt to a wide range of environmental stresses is directly related to the number of two-component systems an organism possesses. Recent advances in this area have identified numerous variations on the a...

journal_title：Molecular microbiology

authors： Desai SK,Kenney LJ

更新日期：2017-01-01 00:00:00

abstract：:The classic expression pattern of the Fis global regulatory protein during batch culture consists of a high peak in the early logarithmic phase of growth, followed by a sharp decrease through mid-exponential growth phase until Fis is almost undetectable at the end of the exponential phase. We discovered that this patt...

journal_title：Molecular microbiology

authors： O Cróinín T,Dorman CJ

更新日期：2007-10-01 00:00:00

abstract：:The Escherichia coli nusG gene product is required for transcription termination by phage HK022 Nun protein at the lambda nutR site in vivo. We show that it is also essential for Nun termination at lambda nutL. Three recessive mis-sense nusG mutations have been isolated that inhibit termination by Nun at lambda nutR. ...

journal_title：Molecular microbiology

authors： Burova E,Hung SC,Chen J,Court DL,Zhou JG,Mogilnitskiy G,Gottesman ME

更新日期：1999-03-01 00:00:00