To ∼P or Not to ∼P? Non-canonical activation by two-component response regulators.


:Bacteria sense and respond to their environment through the use of two-component regulatory systems. The ability to adapt to a wide range of environmental stresses is directly related to the number of two-component systems an organism possesses. Recent advances in this area have identified numerous variations on the archetype systems that employ a sensor kinase and a response regulator. It is now evident that many orphan regulators that lack cognate kinases do not rely on phosphorylation for activation and new roles for unphosphorylated response regulators have been identified. The significance of recent findings and suggestions for further research are discussed.


Mol Microbiol


Molecular microbiology


Desai SK,Kenney LJ




Has Abstract


2017-01-01 00:00:00












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    abstract::The tprK gene sequence of Treponema pallidum subspecies pallidum (T. pallidum) is heterogeneous within and among isolates. Heterogeneity in the tprK open reading frame is localized in seven discrete variable (V) regions, and variability results from apparent base changes, insertions or deletions. The TprK V regions ar...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Centurion-Lara A,LaFond RE,Hevner K,Godornes C,Molini BJ,Van Voorhis WC,Lukehart SA

    更新日期:2004-06-01 00:00:00

  • A nuclear protein NsiA from Epichloë festucae interacts with a MAP kinase MpkB and regulates the expression of genes required for symbiotic infection and hyphal cell fusion.

    abstract::The endophytic fungus Epichloë festucae systemically colonizes the intercellular spaces of cool-season grasses to establish a mutualistic symbiosis. Hyphal growth of the endophyte within the host plant is tightly regulated and synchronized with the growth of the host plant. A genetic screen to identify symbiotic genes...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Tanaka A,Kamiya S,Ozaki Y,Kameoka S,Kayano Y,Saikia S,Akano F,Uemura A,Takagi H,Terauchi R,Maruyama JI,Hammadeh HH,Fleissner A,Scott B,Takemoto D

    更新日期:2020-10-01 00:00:00

  • Suppression of a nuclear frameshift mutation by a mitochondrial tRNA in the yeast Kluyveromyces lactis.

    abstract::A fragment of mitochondrial DNA containing the Kluyveromyces lactis gene for valine-tRNA (tRNAVAL) was isolated as a multicopy suppressor of a respiratory-deficient mutant of this yeast. The mutant produced a truncated Cox14p because of a +1 frameshift mutation in COX14, a nuclear gene encoding a protein imported into...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Fiori A,Francisci S,Falcone C

    更新日期:2002-10-01 00:00:00

  • Aminoacyl-tRNA synthesis in archaea: different but not unique.

    abstract::Accurate aminoacyl-tRNA synthesis is essential for correct translation of the genetic code in all organisms. Whereas many aspects of this process are conserved, others display a surprisingly high level of divergence from the canonical Escherichia coli model system. These differences are most pronounced in archaea wher...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


    authors: Praetorius-Ibba M,Ibba M

    更新日期:2003-05-01 00:00:00

  • Purine biosynthesis in Escherichia coli K12: structure and DNA sequence studies of the purHD locus.

    abstract::The de novo purine biosynthetic enzymes 5-amino-4-imidazolecarboxamide-ribonucleotide (AICAR) transformylase (EC, IMP cyclohydrolase (EC and glycineamide-ribonucleotide (GAR) synthetase (EC are encoded by the purHD locus of Escherichia coli. The DNA sequence of this locus revealed two open ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Flannigan KA,Hennigan SH,Vogelbacker HH,Gots JS,Smith JM

    更新日期:1990-03-01 00:00:00

  • The essential Escherichia coli msbA gene, a multicopy suppressor of null mutations in the htrB gene, is related to the universally conserved family of ATP-dependent translocators.

    abstract::We report the characterization of the msbA gene, isolated as a multicopy suppressor of the HtrB temperature-sensitive phenotype. The msbA gene maps to 20.5 min on the Escherichia coli genetic map and encodes a protein with an estimated molecular mass of 64,460 Da, with the properties of an integral membrane protein. T...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


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    更新日期:1993-01-01 00:00:00

  • A seven-WD40 protein related to human RACK1 regulates mating and virulence in Ustilago maydis.

    abstract::In mammalian cells RACK1 serves as a scaffold protein that has a role in integrating inputs from different signalling pathways and affects translation through association with ribosomes. Ustilago maydis contains a seven-WD40 repeat motif protein designated Rak1, which shows 68% identity to RACK1 and 51% identity to As...

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    pub_type: 杂志文章


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    更新日期:2011-09-01 00:00:00

  • Characterization and modelling of VanT: a novel, membrane-bound, serine racemase from vancomycin-resistant Enterococcus gallinarum BM4174.

    abstract::Sequence determination of a region downstream from the vanXYc gene in Enterococcus gallinarum BM4174 revealed an open reading frame, designated vanT, that encodes a 698-amino-acid polypeptide with an amino-terminal domain containing 10 predicted transmembrane segments. The protein contained a highly conserved pyridoxa...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


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    更新日期:1999-03-01 00:00:00

  • New erythromycin derivatives from Saccharopolyspora erythraea using sugar O-methyltransferases from the spinosyn biosynthetic gene cluster.

    abstract::Using a previously developed expression system based on the erythromycin-producing strain of Saccharopolyspora erythraea, O-methyltransferases from the spinosyn biosynthetic gene cluster of Saccharopolyspora spinosa have been shown to modify a rhamnosyl sugar attached to a 14-membered polyketide macrolactone. The spnI...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


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    更新日期:2001-09-01 00:00:00

  • Cell wall-anchored 5'-nucleotidases in Gram-positive cocci.

    abstract::5'-nucleotidases (5'-NTs) are enzymes that catalyze the hydrolysis of nucleoside monophosphates to produce nucleosides and phosphate. Since the identification of adenosine synthase A (AdsA) in Staphylococcus aureus in 2009, several other 5'-NTs have been discovered in Gram-positive cocci, mainly in streptococci. Despi...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


    authors: Soh KY,Loh JMS,Proft T

    更新日期:2020-04-01 00:00:00

  • Spatiotemporal control of FlgZ activity impacts Pseudomonas aeruginosa flagellar motility.

    abstract::The c-di-GMP-binding effector protein FlgZ has been demonstrated to control motility in the opportunistic pathogen Pseudomonas aeruginosa and it was suggested that c-di-GMP-bound FlgZ impedes motility via its interaction with the MotCD stator. To further understand how motility is downregulated in P. aeruginosa and to...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


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    更新日期:2019-06-01 00:00:00

  • Helicobacter pylori HP0518 affects flagellin glycosylation to alter bacterial motility.

    abstract::Helicobacter pylori is a human gastric pathogen associated with gastric and duodenal ulcers as well as gastric cancer. Mounting evidence suggests this pathogen's motility is prerequisite for successful colonization of human gastric tissues. Here, we isolated an H. pylori G27 HP0518 mutant exhibiting altered motility i...

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    pub_type: 杂志文章


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    更新日期:2010-12-01 00:00:00

  • Ureaplasma antigenic variation beyond MBA phase variation: DNA inversions generating chimeric structures and switching in expression of the MBA N-terminal paralogue UU172.

    abstract::Phase variation of the major ureaplasma surface membrane protein, the multiple-banded antigen (MBA), with its counterpart, the UU376 protein, was recently discussed as a result of DNA inversion occurring at specific inverted repeats. Two similar inverted repeats to the ones within the mba locus were found in the genom...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Zimmerman CU,Rosengarten R,Spergser J

    更新日期:2011-02-01 00:00:00

  • Light-dependent gene activation in Aspergillus nidulans is strictly dependent on phytochrome and involves the interplay of phytochrome and white collar-regulated histone H3 acetylation.

    abstract::The ability for light sensing is found from bacteria to humans but relies only on a small number of evolutionarily conserved photoreceptors. A large number of fungi react to light, mostly to blue light. Aspergillus nidulans also responds to red light using a phytochrome light sensor, FphA, for the control of hundreds ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Hedtke M,Rauscher S,Röhrig J,Rodríguez-Romero J,Yu Z,Fischer R

    更新日期:2015-08-01 00:00:00

  • A novel RNA polymerase-binding protein controlling genes involved in spore germination in Bacillus subtilis.

    abstract::A growing class of proteins regulates transcription through interaction with DNA-dependent RNA polymerase. Here we report that a recently identified, highly conserved sporulation gene ylyA encodes a novel RNA polymerase-binding protein that influences the expression of genes under the control of the late-acting, sporu...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Traag BA,Ramirez-Peralta A,Wang Erickson AF,Setlow P,Losick R

    更新日期:2013-07-01 00:00:00

  • Interaction of the sensor module of Mycobacterium tuberculosis H37Rv KdpD with members of the Lpr family.

    abstract::The genetic and biochemical mechanisms by which Mycobacterium tuberculosis senses and responds to the complex environment that it encounters during infection and persistence within the host remain unknown. In a number of bacterial species, the Kdp signal transduction pathway appears to be the primary response to envir...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Steyn AJ,Joseph J,Bloom BR

    更新日期:2003-02-01 00:00:00

  • Multiple GATA sites: protein binding and physiological relevance for the regulation of the proline transporter gene of Aspergillus nidulans.

    abstract::In Aspergillus nidulans, proline can serve both as a carbon and a nitrogen source. The transcription of the prnB gene, encoding the proline transporter, is efficiently repressed only by the simultaneous presence of ammonium and glucose. Thus, repression of this gene demands the activation of the CreA repressor and the...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Gómez D,García I,Scazzocchio C,Cubero B

    更新日期:2003-10-01 00:00:00

  • Group A streptococcal growth phase-associated virulence factor regulation by a novel operon (Fas) with homologies to two-component-type regulators requires a small RNA molecule.

    abstract::A novel growth phase-associated two-component-type regulator, Fas (fibronectin/fibrinogen binding/haemolytic activity/streptokinase regulator), of Streptococcus pyogenes was identified in the M1 genome sequence, based on homologies to the histidine protein kinase (HPK) and response regulator (RR) part of the Staphyloc...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Kreikemeyer B,Boyle MD,Buttaro BA,Heinemann M,Podbielski A

    更新日期:2001-01-01 00:00:00

  • The periplasmic domains of Vibrio cholerae ToxR and ToxS are forming a strong heterodimeric complex independent on the redox state of ToxR cysteines.

    abstract::The transmembrane protein ToxR plays a key role in the virulence expression system of Vibrio cholerae. The activity of ToxR is dependent on its periplasmic sensor domain (ToxRp) and on the inner membrane protein ToxS. Herein, we present the Nuclear Magnetic Resonance NMR solution structure of the sensory ToxRp contain...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Gubensäk N,Wagner GE,Schrank E,Falsone FS,Berger TMI,Pavkov-Keller T,Reidl J,Zangger K

    更新日期:2020-12-23 00:00:00

  • Charting and unzipping the surface layer of Corynebacterium glutamicum with the atomic force microscope.

    abstract::Bacterial surface layers (S-layers) are extracellular protein networks that act as molecular sieves and protect a large variety of archaea and bacteria from hostile environments. Atomic force microscopy (AFM) was used to asses the S-layer of Coryne-bacterium glutamicum formed of PS2 proteins that assemble into hexamer...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Scheuring S,Stahlberg H,Chami M,Houssin C,Rigaud JL,Engel A

    更新日期:2002-05-01 00:00:00

  • Molecular mechanisms of virstatin resistance by non-O1/non-O139 strains of Vibrio cholerae.

    abstract::Virstatin is a previously described small molecule inhibitor of Vibrio cholerae virulence. We have demonstrated that the molecule inhibits the activity of the transcriptional activator ToxT, thereby preventing elaboration of the toxin co-regulated pilus (TCP) and cholera toxin in vitro and in vivo in O1 strains of V. ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Shakhnovich EA,Sturtevant D,Mekalanos JJ

    更新日期:2007-12-01 00:00:00

  • SaPI mutations affecting replication and transfer and enabling autonomous replication in the absence of helper phage.

    abstract::The SaPIs are chromosomal islands in staphylococci and other Gram-positive bacteria that carry genes for superantigens, virulence factors, resistance and certain metabolic functions. They have intimate relationships with certain temperate phages involving phage-induced excision, replication and efficient packaging in ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Ubeda C,Maiques E,Barry P,Matthews A,Tormo MA,Lasa I,Novick RP,Penadés JR

    更新日期:2008-02-01 00:00:00

  • Novel carbohydrate binding modules in the surface anchored α-amylase of Eubacterium rectale provide a molecular rationale for the range of starches used by this organism in the human gut.

    abstract::Gut bacteria recognize accessible glycan substrates within a complex environment. Carbohydrate binding modules (CBMs) of cell surface glycoside hydrolases often drive binding to the target substrate. Eubacterium rectale, an important butyrate-producing organism in the gut, consumes a limited range of substrates, inclu...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Cockburn DW,Suh C,Medina KP,Duvall RM,Wawrzak Z,Henrissat B,Koropatkin NM

    更新日期:2018-01-01 00:00:00

  • Many of the genes required for mating in Saccharomyces cerevisiae are also required for mating in Candida albicans.

    abstract::Candida albicans is the single, most frequently isolated human fungal pathogen. As with most fungal pathogens, the factors which contribute to pathogenesis in C. albicans are not known, despite more than a decade of molecular genetic analysis. Candida albicans was thought to be asexual until the discovery of the MTL l...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Magee BB,Legrand M,Alarco AM,Raymond M,Magee PT

    更新日期:2002-12-01 00:00:00

  • The β₃-integrin ligand of Borrelia burgdorferi is critical for infection of mice but not ticks.

    abstract::P66 is a Borrelia burgdorferi surface protein with β₃ integrin binding and channel forming activities. In this study, the role of P66 in mammalian and tick infection was examined. B. burgdorferiΔp66 strains were not infectious in wild-type, TLR2⁻/⁻- or MyD88⁻/⁻-deficient mice. Strains with p66 restored to the chromoso...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Ristow LC,Miller HE,Padmore LJ,Chettri R,Salzman N,Caimano MJ,Rosa PA,Coburn J

    更新日期:2012-09-01 00:00:00

  • Functional analysis of the Bacillus subtilis morphogenetic spore coat protein CotE.

    abstract::Bacterial spores are surrounded by a multilayered proteinaceous shell called the coat. In Bacillus subtilis, a coat protein called CotE guides the assembly of a major subset of coat proteins. To understand how CotE carries out its role in coat morphogenesis, we subjected its gene to mutagenesis and studied the effects...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Little S,Driks A

    更新日期:2001-11-01 00:00:00

  • Replication mutants of Staphylococcus aureus macrolide-lincosamide-streptogramin B resistance plasmid pT48.

    abstract::Copy-number mutants of Staphylococcus aureus macrolide-lincosamide-streptogramin B (MLS) resistance plasmid pT48 were isolated by their resistance to the non-inducing macrolide, tylosin. One mutant plasmid, pcopD3, showed a three- to five-fold cis-dominant increase in copy number, and nucleotide sequence analysis reve...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Catchpole I,Dyke KG

    更新日期:1991-04-01 00:00:00

  • Experimental genome evolution: large-scale genome rearrangements associated with resistance to replacement of a chromosomal restriction-modification gene complex.

    abstract::Type II restriction enzymes are paired with modification enzymes that protect type II restriction sites from cleavage by methylating them. A plasmid carrying a type II restriction-modification gene complex is not easily replaced by an incompatible plasmid because loss of the former leads to cell death through chromoso...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Handa N,Nakayama Y,Sadykov M,Kobayashi I

    更新日期:2001-05-01 00:00:00

  • Characterization of integration host factor (IHF) binding upstream of the cysteine-rich protein operon (omcAB) promoter of Chlamydia trachomatis LGV serovar L2.

    abstract::Chlamydiae are bacterial parasites that carry out a distinct developmental cycle within host cells; however, the mechanisms by which these organisms regulate stage-specific gene expression are not known. We identified a DNA element located between nucleotide (nt) -135 and -90 upstream from the transcription start poin...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Zhong J,Douglas AL,Hatch TP

    更新日期:2001-07-01 00:00:00

  • Candida albicans lacking the frataxin homologue: a relevant yeast model for studying the role of frataxin.

    abstract::We cloned the CaYFH1 gene that encodes the yeast frataxin homologue in Candida albicans. CaYFH1 was expressed in Deltayfh1 Saccharomyces cerevisiae cells, where it compensated for all the phenotypes tested except for the lack of cytochromes. Double DeltaCayfh1/DeltaCayfh1 mutant had severe defective growth, accumulate...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Santos R,Buisson N,Knight SA,Dancis A,Camadro JM,Lesuisse E

    更新日期:2004-10-01 00:00:00