To ∼P or Not to ∼P? Non-canonical activation by two-component response regulators.

abstract：:In the past, ultrastructural investigations of Leishmania mexicana amastigotes revealed structures that were tentatively identified as autophagosomes. This study has now provided definitive data that autophagy occurs in the parasite during differentiation both to metacyclic promastigotes and to amastigotes, autophagos...

journal_title：Molecular microbiology

authors： Williams RA,Tetley L,Mottram JC,Coombs GH

更新日期：2006-08-01 00:00:00

abstract：:Helicobacter pylori strains isolated from most patients with peptic ulcer disease and adenocarcinoma express the vacuolating toxin VacA and contain a pathogenicity island named cag. The cag pathogenicity island codes for more than 40 putative proteins with features similar to bacterial secretion systems. One of these ...

journal_title：Molecular microbiology

authors： Spohn G,Beier D,Rappuoli R,Scarlato V

更新日期：1997-10-01 00:00:00

abstract：:The bacterial phosphoenolpyruvate-dependent phosphotransferase system (PTS) consists of a set of cytoplasmic energy-coupling proteins and various integral membrane permeases/sugar phosphotransferases, each specific for a different sugar. We have conducted biochemical analyses of three PTS permeases (enzymes II), the g...

journal_title：Molecular microbiology

authors： Aboulwafa M,Saier M Jr

更新日期：2003-04-01 00:00:00

abstract：:The Helicobacter pylori vacuolating cytotoxin (VacA) induces degenerative vacuolation of sensitive mammalian cell lines. Although evidence is accumulating that VacA enters cells and functions from an intracellular site of action, the biochemical mechanism by which VacA mediates cellular vacuolation has not been establ...

journal_title：Molecular microbiology

authors： Ye D,Blanke SR

更新日期：2002-03-01 00:00:00

abstract：:Sinorhizobium meliloti is a soil bacterium that invades the root nodules it induces on Medicago sativa, whereupon it undergoes an alteration of its cell cycle and differentiates into nitrogen-fixing, elongated and polyploid bacteroid with higher membrane permeability. In Caulobacter crescentus, a related alphaproteoba...

journal_title：Molecular microbiology

authors： Pini F,Frage B,Ferri L,De Nisco NJ,Mohapatra SS,Taddei L,Fioravanti A,Dewitte F,Galardini M,Brilli M,Villeret V,Bazzicalupo M,Mengoni A,Walker GC,Becker A,Biondi EG

更新日期：2013-10-01 00:00:00

abstract：:We show that Yersinia pestis and pesticin-sensitive isolates of Y. pseudotuberculosis possess a common 34 kbp DNA region that has all the hallmarks of a pathogenicity island and is inserted into different asparaginyl tRNA genes at different chromosomal locations in each species. This pathogenicity island (YP-HPI) is m...

journal_title：Molecular microbiology

authors： Hare JM,Wagner AK,McDonough KA

更新日期：1999-01-01 00:00:00

abstract：:We report here the cloning of the arginine repressor gene argR of Bacillus stearothermophilus and the characterization and purification to homogeneity of its product. The deduced amino acid sequence of the 16.8-kDa ArgR subunit shares 72% identity with its mesophilic homologue AhrC of Bacilus subtilis. Sequence analys...

journal_title：Molecular microbiology

authors： Dion M,Charlier D,Wang H,Gigot D,Savchenko A,Hallet JN,Glansdorff N,Sakanyan V

更新日期：1997-07-01 00:00:00

abstract：:Reverse transcriptase, discovered in 1970 in retroviruses, has until recently been found only in eukaryotic organisms. Recently it was shown to occur in two groups of bacteria: myxobacteria and Escherichia coli. The gene for reverse transcriptase is part of a chromosomal genetic element that codes for the production o...

journal_title：Molecular microbiology

authors： Lim D,Maas WK

更新日期：1989-08-01 00:00:00

abstract：:In the mouse model for systemic infection, natural a/alpha strains of C. albicans are more virulent and more competitive than their spontaneous MTL-homozygous offspring, which arise primarily by loss of one chromosome 5 homologue followed by duplication of the retained homologue (uniparental disomy). Deletion of eithe...

journal_title：Molecular microbiology

authors： Wu W,Lockhart SR,Pujol C,Srikantha T,Soll DR

更新日期：2007-06-01 00:00:00

abstract：:Genetic exchange by natural transformation in Streptococcus pneumoniae occurs in a cell-density dependent process and is initiated by a small extracellular signalling molecule, the competence-stimulating peptide (CSP). comC, the gene for this peptide, has previously been identified and encodes a 44 amino acid pre-pept...

journal_title：Molecular microbiology

authors： Cheng Q,Campbell EA,Naughton AM,Johnson S,Masure HR

更新日期：1997-02-01 00:00:00

abstract：:Platelet binding by Streptococcus gordonii strain M99 is strongly correlated with the expression of the large surface glycoprotein GspB. A 14 kb chromosomal region downstream of gspB was previously shown to be required for the expression of this protein. The region encodes SecA2 and SecY2, which are components of an a...

journal_title：Molecular microbiology

authors： Takamatsu D,Bensing BA,Sullam PM

更新日期：2004-04-01 00:00:00

abstract：:Two-component signal transduction (TCST) pathways are regulatory systems that are highly homologous throughout the bacterial kingdom. Their established role in virulence and absence in vertebrates has made TCST an attractive target for therapeutic intervention. However, such systems have yet to yield success in the de...

journal_title：Molecular microbiology

authors： Hubbard JA,MacLachlan LK,King GW,Jones JJ,Fosberry AP

更新日期：2003-09-01 00:00:00

abstract：:When fungi grow on plant or insect surfaces coated with wax polyesters that protect against pathogens, the fungi generally form aerial hyphae to contact the surfaces. Aerial structures such as hyphae and conidiophores are coated with hydrophobins, which are surface-active proteins involved in adhesion to hydrophobic s...

journal_title：Molecular microbiology

authors： Takahashi T,Maeda H,Yoneda S,Ohtaki S,Yamagata Y,Hasegawa F,Gomi K,Nakajima T,Abe K

更新日期：2005-09-01 00:00:00

abstract：:We have identified and characterized three genes, SUT1, SUT2 and SUT3, that encode glucose transporters of the yeast Pichia stipitis. When expressed in a Saccharomyces cerevisiae hxt null mutant strain that is unable to take up monosaccharides, all three proteins restored growth on glucose. Sequencing of the genes rev...

journal_title：Molecular microbiology

authors： Weierstall T,Hollenberg CP,Boles E

更新日期：1999-02-01 00:00:00

abstract：:Pseudomonas aeruginosa releases several extracellular proteins which are secreted via two independent secretion pathways. Alkaline protease (AprA) Is released by its own specific secretion machinery which is an ABC-transporter. Despite sequence similarities between components of ABC-transporters in different bacteria,...

journal_title：Molecular microbiology

authors： Duong F,Lazdunski A,Murgier M

更新日期：1996-08-01 00:00:00

abstract：:Gut bacteria recognize accessible glycan substrates within a complex environment. Carbohydrate binding modules (CBMs) of cell surface glycoside hydrolases often drive binding to the target substrate. Eubacterium rectale, an important butyrate-producing organism in the gut, consumes a limited range of substrates, inclu...

journal_title：Molecular microbiology

authors： Cockburn DW,Suh C,Medina KP,Duvall RM,Wawrzak Z,Henrissat B,Koropatkin NM

更新日期：2018-01-01 00:00:00

abstract：:The NarL and NarP proteins are homologous response regulators of Escherichia coli that control the expression of several operons in response to nitrate and nitrite. A consensus heptameric NarL DNA-binding sequence has been identified, and previous observations suggest that the NarP protein has a similar sequence speci...

journal_title：Molecular microbiology

authors： Darwin AJ,Tyson KL,Busby SJ,Stewart V

更新日期：1997-08-01 00:00:00

abstract：:Cell cycle transitions are often triggered by the proteolysis of key regulatory proteins. In Caulobacter crescentus, the G1-S transition involves the degradation of an essential DNA-binding response regulator, CtrA, by the ClpXP protease. Here, we show that another critical cell cycle regulator, SciP, is also degraded...

journal_title：Molecular microbiology

authors： Gora KG,Cantin A,Wohlever M,Joshi KK,Perchuk BS,Chien P,Laub MT

更新日期：2013-03-01 00:00:00

abstract：:The monocistronic transcript of rpsO undergoes an endonucleolytic cleavage downstream of the coding sequence, which removes the hairpin of the transcription terminator and initiates the rapid degradation of the message. We demonstrate here that the two rne-dependent cleavages, on both sides of the transcription termin...

journal_title：Molecular microbiology

authors： Braun F,Hajnsdorf E,Régnier P

更新日期：1996-03-01 00:00:00

abstract：:Messenger RNA decay in Bacillus subtilis is accomplished by a combination of exoribonucleases and endoribonucleases. Intermediates in the decay process have not been readily detectable, and previous studies on mRNA decay have used a handful of highly expressed transcripts as models. Here, we use RNA-Seq analysis to pr...

journal_title：Molecular microbiology

authors： Liu B,Deikus G,Bree A,Durand S,Kearns DB,Bechhofer DH

更新日期：2014-10-01 00:00:00

abstract：:Alternative oxidase (AOX) is a respiratory oxidase found in certain eukaryotes and bacteria; however, its role in bacterial physiology is unclear. Exploiting the genetic tractability of the bacterium Vibrio fischeri, we explore the regulation of aox expression and AOX function. Using quantitative PCR and reporter assa...

journal_title：Molecular microbiology

authors： Dunn AK,Karr EA,Wang Y,Batton AR,Ruby EG,Stabb EV

更新日期：2010-07-01 00:00:00

abstract：:The recently identified recA gene of the naturally transformable bacterium Streptococcus pneumoniae has been further characterized by constructing a recA null mutation and by investigating its regulation. The recA mutation has been shown to confer both DNA repair (as judged from sensitivity to u.v. and methyl methane ...

journal_title：Molecular microbiology

authors： Martin B,García P,Castanié MP,Claverys JP

更新日期：1995-01-01 00:00:00

abstract：:ICEMlSym(R7A) of Mesorhizobium loti is an integrative and conjugative element (ICE) that confers the ability to form a nitrogen-fixing symbiosis with Lotus species. Horizontal transfer is activated by TraR and N-acyl-homoserine lactone (AHL), which can stimulate ICE excision in 100% of cells. However, in wild-type cu...

journal_title：Molecular microbiology

authors： Ramsay JP,Major AS,Komarovsky VM,Sullivan JT,Dy RL,Hynes MF,Salmond GP,Ronson CW

更新日期：2013-01-01 00:00:00

abstract：:In Saccharomyces cerevisiae MATa cells, export of the a-factor mating pheromone is mediated by Ste6p, a member of the ATP-binding cassette (ABC) superfamily of transporters and a close homologue of mammalian multidrug transporter P-glycoproteins (Pgps). We have used functional complementation of a ste6delta mutation t...

journal_title：Molecular microbiology

authors： Raymond M,Dignard D,Alarco AM,Mainville N,Magee BB,Thomas DY

更新日期：1998-02-01 00:00:00

abstract：:GS-1 (ncu04189) is a protein required for the synthesis of beta-1,3-glucan in Neurospora crassa. As chitin, beta-1,3-glucan is a morphogenetically relevant component of the fungal cell wall. Previously, we showed that chitin synthases are delivered to the growing hyphal tip of N. crassa by secretory microvesicles that...

journal_title：Molecular microbiology

authors： Verdín J,Bartnicki-Garcia S,Riquelme M

更新日期：2009-12-01 00:00:00

abstract：:Overexpression of the MDR1 gene, encoding a multi-drug efflux pump of the major facilitator superfamily, is a major cause of resistance to the widely used antifungal agent fluconazole and other toxic substances in the fungal pathogen Candida albicans. We found that all tested clinical and in vitro generated C. albican...

journal_title：Molecular microbiology

authors： Dunkel N,Blass J,Rogers PD,Morschhäuser J

更新日期：2008-08-01 00:00:00

abstract：:Streptococcus lactis NCDO763 harbours a plasmid designated pLP763. The cells harbouring pLP763 are able to grow to a higher density in milk because of their proteinase-positive phenotype (Prt+). The 6.2 kb HindIII-PstI fragment from pLP763 was found to be responsible for the Prt+ phenotype. The DNA fragment contains a...

journal_title：Molecular microbiology

authors： Kiwaki M,Ikemura H,Shimizu-Kadota M,Hirashima A

更新日期：1989-03-01 00:00:00

abstract：:Many types of microorganisms, including both prokaryotes and eukaryotes, have developed mechanisms to adapt to severe osmotic stress. In this study, we isolated multicopy suppressor genes for a Schizosaccharomyces pombe mutant, which exhibited the clear phenotype of being osmosensitive for growth (Osms) on agar plates...

journal_title：Molecular microbiology

authors： Ohmiya R,Yamada H,Nakashima K,Aiba H,Mizuno T

更新日期：1995-12-01 00:00:00

abstract：:Previous studies with purified variants of the 434 repressor having different operator-binding specificities have demonstrated the interactions of a heterodimeric repressor with a hybrid operator site. The present study investigates the interactions between the 434 repressor and its operator site. The optimum 434 oper...

journal_title：Molecular microbiology

authors： Webster C,Merryweather A,Brammar W

更新日期：1992-02-01 00:00:00

abstract：:Lactose is an abundant dietary carbohydrate metabolized by the dental pathogen Streptococcus mutans. Lactose metabolism presents both classic diauxic behaviors and long-term memory, where the bacteria can pause for >11 h before initiating growth on lactose. Here, we explored mechanisms contributing to unusual aspects ...

journal_title：Molecular microbiology

authors： Zeng L,Burne RA

更新日期：2020-09-03 00:00:00