Abstract:
:Entamoeba histolytica cells secrete electron-dense granules (EDGs) that have collagenase activity. To study the possible involvement of calmodulin (CaM) on EDG secretion, the effect of several CaM antagonists (TFP, R24571, W-7, W-5, dibucaine and DL-propranolol) was tested on this cellular function. Except for W-5 and dibucaine, the rest of these compounds inhibited EDG secretion. Transmission electron microscopy of collagen-activated trophozoites showed numerous EDGs located in or near the surface membrane. In contrast, trophozoites incubated with TFP showed no EDGs. Protein kinase C inhibitors (H-7, ML-9) had no effect on EDG secretion, suggesting that CaM antagonists acted by selectively inhibiting CaM. These results suggest that a CaM-dependent process is involved in EDG secretion.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Muñoz Mde L,Moreno MA,Pérez-Garcia JN,Tovar GR,Hernandez VIdoi
10.1111/j.1365-2958.1991.tb01919.xsubject
Has Abstractpub_date
1991-07-01 00:00:00pages
1707-14issue
7eissn
0950-382Xissn
1365-2958journal_volume
5pub_type
杂志文章abstract::A number of plasmid-encoded gene systems are thought to stabilize plasmids by killing plasmid-free cells (also termed post-segregational killing or plasmid addiction). Here we analyse the mechanisms of plasmid stabilization by ccd of F, parDE of RP4 and parD of R1, and compare them to hok/sok of R1. To induce synchron...
journal_title:Molecular microbiology
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更新日期:1996-09-01 00:00:00
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pub_type: 杂志文章
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journal_title:Molecular microbiology
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journal_title:Molecular microbiology
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pub_type: 杂志文章
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更新日期:2011-02-01 00:00:00
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pub_type: 杂志文章
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pub_type: 评论,杂志文章
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