Abstract:
:In order to reveal functional properties of recombination involving short ssDNAs in hyperthermophilic archaea, we evaluated oligonucleotide-mediated transformation (OMT) in Sulfolobus acidocaldarius and Escherichia coli as a function of the molecular properties of the ssDNA substrates. Unmodified ssDNAs as short as 20-22 nt yielded recombinants in both organisms, as did longer DNAs forming as few as 2-5 base pairs on one side of the genomic mutation. The two OMT systems showed similar responses to certain end modifications of the oligonucleotides, but E. coli was found to require a 5' phosphate on 5'-limited ssDNA whereas this requirement was not evident in S. acidocaldarius. The ability of both E. coli and S. acidocaldarius to incorporate short, mismatched ssDNAs into their genomes raises questions about the biological significance of this capability, including its phylogenetic distribution among microorganisms and its impact on genome stability. These questions seem particularly relevant for S. acidocaldarius, as this archaeon has natural competence for OMT, encodes no MutSL homologues and thrives under environmental conditions that accelerate DNA decomposition.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Grogan DW,Stengel KRdoi
10.1111/j.1365-2958.2008.06356.xsubject
Has Abstractpub_date
2008-09-01 00:00:00pages
1255-65issue
5eissn
0950-382Xissn
1365-2958pii
MMI6356journal_volume
69pub_type
杂志文章abstract::We used artificial chromosome inversions to investigate the chromosomal constraints that preserve genome organization in the Gram-positive bacterium Lactococcus lactis. Large inversions, 80-1260 kb in length, disturbing the symmetry of the origin and terminus of the replication axis to various extents, were constructe...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2003.03847.x
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doi:10.1111/j.1365-2958.2008.06170.x
更新日期:2008-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1046/j.1365-2958.2003.03505.x
更新日期:2003-06-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2014-05-01 00:00:00
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更新日期:2004-10-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2005.04554.x
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更新日期:2004-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-04-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2005.04506.x
更新日期:2005-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1365-2958.2006.05495.x
更新日期:2007-01-01 00:00:00
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更新日期:2007-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/mmi.12987
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pub_type: 杂志文章
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更新日期:2001-07-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章,评审
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更新日期:2006-10-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.13653
更新日期:2017-06-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1994-03-01 00:00:00
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更新日期:2011-03-01 00:00:00
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pub_type: 杂志文章
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journal_title:Molecular microbiology
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pub_type: 杂志文章,评审
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更新日期:2001-09-01 00:00:00
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更新日期:2011-10-01 00:00:00
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更新日期:2013-03-01 00:00:00
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更新日期:2000-11-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1992-07-01 00:00:00
abstract::The crystal structures of two active forms of dissimilatory sulphite reductase (Dsr) from Desulfovibrio gigas, Dsr-I and Dsr-II, are compared at 1.76 and 2.05 Å resolution respectively. The dimeric α2β2γ2 structure of Dsr-I contains eight [4Fe-4S] clusters, two saddle-shaped sirohaems and two flat sirohydrochlorins. I...
journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2010-12-01 00:00:00
abstract::The MtrCDE multidrug pump, from Neisseria gonorrhoeae, is assembled from the inner and outer membrane proteins MtrD and MtrE, which are connected by the periplasmic membrane fusion protein MtrC. Although it is clear that MtrD delivers drugs to the channel of MtrE, it remains unclear how drug delivery and channel openi...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.12211
更新日期:2013-05-01 00:00:00