Biochemical characterization of Leishmania major aquaglyceroporin LmAQP1: possible role in volume regulation and osmotaxis.

abstract：:Streptococcus pneumoniae is one of the most important model organisms for studies on natural genetic transformation in bacteria. The prevalence of this gene exchange mechanism in the genus Streptococcus has not been subjected to systematic investigations, but it has been known for decades that only a few streptococcal...

journal_title：Molecular microbiology

authors： Håvarstein LS

更新日期：2010-11-01 00:00:00

abstract：:Three u.v.-induced mutants of the purine-cytosine permease gene of Saccharomyces cerevisiae, with altered apparent Michaelis constant of transport (Kmapp), were cloned and sequenced. One of the mutants had extensive nucleotide replacement, whereas the other two had a single mutation. To evaluate the contribution of th...

journal_title：Molecular microbiology

authors： Bloch JC,Sychrova H,Souciet JL,Jund R,Chevallier MR

更新日期：1992-10-01 00:00:00

abstract：:Escherichia coli K-12 strain PS1-28-37 carries the multicopy plasmid pPSO28-37 containing a DNA fragment coding for two of the proteins that enable bacteria to utilize sucrose as sole carbon source. One of the different gene products of the plasmid is the outer membrane protein, ScrY. This protein was isolated and pur...

journal_title：Molecular microbiology

authors： Schülein K,Schmid K,Benzl R

更新日期：1991-09-01 00:00:00

abstract：:The efficiency of conjugation of F-like plasmids is regulated by the FinOP fertility inhibition system. The transfer (tra) operon is under the direct control of the TraJ transcriptional activator which, in turn, is negatively regulated by FinP, an antisense RNA, and FinO, a 22 kDa protein. Recently, FinO has been show...

journal_title：Molecular microbiology

authors： van Biesen T,Söderbom F,Wagner EG,Frost LS

更新日期：1993-10-01 00:00:00

abstract：:Dimerization and inactivation of ribosomes in Escherichia coli is a two-step process that involves the binding of ribosome modulation factor (RMF) and hibernation promotion factor (HPF). Lactococcus lactis MG1363 expresses a protein, YfiA(L) (l) , which associates with ribosomes in the stationary phase of growth and i...

journal_title：Molecular microbiology

authors： Puri P,Eckhardt TH,Franken LE,Fusetti F,Stuart MC,Boekema EJ,Kuipers OP,Kok J,Poolman B

更新日期：2014-01-01 00:00:00

abstract：:Two so-called Ter sites, which bind the Escherichia coli Tus protein, are located near the replication origin of plasmid R1. Inactivation of the tus gene caused a large decrease in the stability of maintenance of the R1 mini-derivative pOU47 despite the presence of a functional partition system on the plasmid. Deletio...

journal_title：Molecular microbiology

authors： Krabbe M,Zabielski J,Bernander R,Nordström K

更新日期：1997-05-01 00:00:00

abstract：:The S box transcription termination control system regulates expression of genes involved in methionine metabolism. Expression of the S box regulon, comprised of 11 transcriptional units in Bacillus subtilis, is induced in response to starvation for methionine. We previously demonstrated that S-adenosylmethionine (SAM...

journal_title：Molecular microbiology

authors： McDaniel BA,Grundy FJ,Henkin TM

更新日期：2005-08-01 00:00:00

abstract：:Five amino acids (Y105, Y176, Y189, Y189, W207) that constitute the substrate binding site of PHB depolymerase PhaZ7 were identified. All residues are located at a single surface-exposed location of PhaZ7. Exchange of these amino acids by less hydrophobic, hydrophilic or negatively charged residues reduced binding of ...

journal_title：Molecular microbiology

authors： Jendrossek D,Hermawan S,Subedi B,Papageorgiou AC

更新日期：2013-11-01 00:00:00

abstract：:Antigenic variation in Trypanosoma brucei has selected for the evolution of a massive archive of silent Variant Surface Glycoprotein (VSG) genes, which are activated by recombination into specialized expression sites. Such VSG switching can occur at rates substantially higher than background mutation and is dependent ...

journal_title：Molecular microbiology

authors： Hartley CL,McCulloch R

更新日期：2008-06-01 00:00:00

abstract：:Helicobacter pylori persists deep in the human gastric mucus layer in a harsh, nutrient-poor environment. Survival under these conditions depends on the ability of this human pathogen to invoke starvation/stress responses when needed. Unlike many bacteria, H. pylori lacks starvation/stress-responding alternative sigma...

journal_title：Molecular microbiology

authors： Yang ZX,Zhou YN,Yang Y,Jin DJ

更新日期：2010-08-01 00:00:00

abstract：:Various physiological stimuli trigger the conversion of noninfective Leishmania donovani promastigotes to the infective form. Here, we present the first evidence of the effect of glucose starvation, on virulence and survival of these parasites. Glucose starvation resulted in a decrease in metabolically active parasite...

journal_title：Molecular microbiology

authors： Saini S,Kumar Ghosh A,Singh R,Das S,Abhishek K,Kumar A,Verma S,Mandal A,Hasan Sardar A,Purkait B,Kumar A,Kumar Sinha K,Das P

更新日期：2016-12-01 00:00:00

abstract：:In Candida glabrata, the sirtuins Sir2 and Hst1 control the expression of a wide number of genes including adhesins required for host colonization and niacin transporters needed for growth. Given that these sirtuins can be inactivated during infection, we asked if their inhibition could modify the response of C. glabr...

journal_title：Molecular microbiology

authors： Orta-Zavalza E,Guerrero-Serrano G,Gutiérrez-Escobedo G,Cañas-Villamar I,Juárez-Cepeda J,Castaño I,De Las Peñas A

更新日期：2013-06-01 00:00:00

abstract：:Expression of the cysteine regulon in Salmonella typhimurium and Escherichia coli is controlled by the LysR-type transcriptional activator CysB and by the inducer N-acetyl-L-serine. Sulphide and thiosulphate are anti-inducers. Two highly purified constitutive CysB proteins, CysB(T149M) and CysB(T149P), were found to b...

journal_title：Molecular microbiology

authors： Colyer TE,Kredich NM

更新日期：1996-07-01 00:00:00

abstract：:RelA and SpoT of Gram-negative organisms critically regulate cellular levels of (p)ppGpp. Here, we have dissected the spoT gene function of the cholera pathogen Vibrio cholerae by extensive genetic analysis. Unlike Escherichia coli, V. choleraeDeltarelADeltaspoT cells accumulated (p)ppGpp upon fatty acid or glucose st...

journal_title：Molecular microbiology

authors： Das B,Pal RR,Bag S,Bhadra RK

更新日期：2009-04-01 00:00:00

abstract：:The Helicobacter pylori genome contains a gene (hp1338 or nikR) that encodes a nickel-dependent regulator that is homologous to the Escherichia coli nickel-responsive regulator, NikR. The H. pylori nikR product acts as a pleiotropic metal-dependent regulator. We constructed a non-polar isogenic mutant deleted for the ...

journal_title：Molecular microbiology

authors： Contreras M,Thiberge JM,Mandrand-Berthelot MA,Labigne A

更新日期：2003-08-01 00:00:00

abstract：:The identical partial deep-core structure of Hep alpha 1-3Hep alpha 1-5KDO in Salmonella typhimurium LT2 LPS and Neisseria gonorrhoeae LOS enabled us to isolate a DNA fragment from N. gonorrhoeae that was able to complement the alpha 1,5 LOS heptosyltransferase defect in the S. typhimurium rfaC630 (SA1377) mutant. SDS...

journal_title：Molecular microbiology

authors： Zhou D,Lee NG,Apicella MA

更新日期：1994-11-01 00:00:00

abstract：:Crystallographic structure refinement at very high resolutions of a dozen periplasmic receptors has revealed that, though they have different sizes (26 to 60 kDa) and little sequence homology, they have high tertiary structure similarity. They consist of two distinct globular domains bisected by a cleft or groove wher...

journal_title：Molecular microbiology

authors： Quiocho FA,Ledvina PS

更新日期：1996-04-01 00:00:00

abstract：:Legionella pneumophila can replicate inside amoebae and also alveolar macrophages to cause Legionnaires' Disease in susceptible hosts. When nutrients become limiting, a stringent-like response coordinates the differentiation of L. pneumophila to a transmissive form, a process mediated by the two-component system LetA/...

journal_title：Molecular microbiology

authors： Molofsky AB,Swanson MS

更新日期：2003-10-01 00:00:00

abstract：:Pseudomonas syringae pv. tomato (Pst) strain DC3000 infects the model plants Arabidopsis thaliana and tomato, causing disease symptoms characterized by necrotic lesions surrounded by chlorosis. One mechanism used by Pst DC3000 to infect host plants is the type III protein secretion system, which is thought to deliver ...

journal_title：Molecular microbiology

authors： Zwiesler-Vollick J,Plovanich-Jones AE,Nomura K,Bandyopadhyay S,Joardar V,Kunkel BN,He SY

更新日期：2002-09-01 00:00:00

abstract：:Reactive oxygen species (ROS) are critical components of the antimicrobial repertoire of macrophages, yet the mechanisms by which ROS damage bacteria in the phagosome are unclear. The NADH-dependent phagocytic oxidase produces superoxide, which dismutes to form H(2)O(2). The Barras and Méresse labs use a GFP fusion to...

journal_title：Molecular microbiology

authors： Slauch JM

更新日期：2011-05-01 00:00:00

abstract：:Renewed interest in gene amplification stems from its importance in evolution and a variety of medical problems ranging from drug resistance to cancer. However, amplified DNA segments (amplicons) are not fully characterized in any organism. Here we report a novel Acinetobacter baylyi system for genome-wide studies. Am...

journal_title：Molecular microbiology

authors： Seaton SC,Elliott KT,Cuff LE,Laniohan NS,Patel PR,Neidle EL

更新日期：2012-02-01 00:00:00

abstract：:Listeria monocytogenes FrvA (Lmo0641) is critical for virulence in the mouse model and is an ortholog of the Bacillus subtilis Fur- and PerR-regulated Fe(II) efflux P1B4 -type ATPase PfeT. Previously, FrvA was suggested to protect against heme toxicity. Here, we demonstrate that an frvA mutant is sensitive to iron int...

journal_title：Molecular microbiology

authors： Pi H,Patel SJ,Argüello JM,Helmann JD

更新日期：2016-06-01 00:00:00

abstract：:Each year, several million tons of dimethylsulfoniopropionate (DMSP) are produced by marine phytoplankton and bacteria as an important osmolyte to regulate their cellular osmosis. Microbial breakdown of DMSP to the volatile gas dimethylsulfide (DMS) plays an important role in global biogeochemical cycles of the sulphu...

journal_title：Molecular microbiology

authors： Chen Y,Schäfer H

更新日期：2019-06-01 00:00:00

abstract：:The group A streptococcus (GAS) causes a variety of human diseases, including toxic shock syndrome and necrotizing fasciitis, which are both associated with significant mortality. Even the superficial self-limiting diseases caused by GAS, such as pharyngitis, impose a significant economic burden on society. GAS can ca...

journal_title：Molecular microbiology

authors： Churchward G

更新日期：2007-04-01 00:00:00

abstract：:Protein phosphatases 2C are a family of conserved enzymes involved in many aspects of the cell biology. We reported that, in the yeast Saccharomyces cerevisiae, overexpression of the Ptc3p isoform resulted in increased lithium tolerance in the hypersensitive hal3 background. We have found that the tolerance induced by...

journal_title：Molecular microbiology

authors： Ruiz A,González A,García-Salcedo R,Ramos J,Ariño J

更新日期：2006-10-01 00:00:00

abstract：:We report a novel strategy for selecting mutations that mislocalize lipoproteins within the Escherichia coli cell envelope and describe the mutants obtained. A strain carrying a deletion of the chromosomal malE gene, coding for the periplasmic maltose-binding protein (MalE), cannot use maltose unless a wild-type copy ...

journal_title：Molecular microbiology

authors： Seydel A,Gounon P,Pugsley AP

更新日期：1999-11-01 00:00:00

abstract：:The earliest event in bacterial cell division is the formation of a Z ring, composed of the tubulin-like FtsZ protein, at the division site at midcell. This ring then recruits several other division proteins and together they drive the formation of a division septum between two replicated chromosomes. Here we show tha...

journal_title：Molecular microbiology

authors： Peters PC,Migocki MD,Thoni C,Harry EJ

更新日期：2007-04-01 00:00:00

abstract：:The Incl1 conjugative plasmid Collb-P9 carries a psiB gene that prevents induction of the SOS response in host bacteria. This locus is located 2.5 kb downstream of the ssb (single-stranded DNA-binding protein) gene in the leading region. This portion of Collb is strikingly similar to part of the leading region of the ...

journal_title：Molecular microbiology

authors： Jones AL,Barth PT,Wilkins BM

更新日期：1992-03-01 00:00:00

abstract：:A region of 7.8 kb of the plasmid pMB2 from Enterococcus faecalis S-48 carrying the information necessary for production and immunity of the peptide antibiotic AS-48 has been cloned and sequenced. It contains the as-48A structural gene plus five open reading frames (as-48B, as-48C, as-48C1, as-48D and as-48D1). Beside...

journal_title：Molecular microbiology

authors： Martínez-Bueno M,Valdivia E,Gálvez A,Coyette J,Maqueda M

更新日期：1998-01-01 00:00:00

abstract：:During persistent infection, the intracellular bacterial pathogen Chlamydia trachomatis is viable but severely attenuates the production of new, infectious elementary bodies (EBs). To investigate the reasons for this lack of new EB output, we analysed the expression of chlamydial genes encoding products required for D...

journal_title：Molecular microbiology

authors： Gérard HC,Krausse-Opatz B,Wang Z,Rudy D,Rao JP,Zeidler H,Schumacher HR,Whittum-Hudson JA,Köhler L,Hudson AP

更新日期：2001-08-01 00:00:00