Peptide and amino acid metabolism is controlled by an OmpR-family response regulator in Lactobacillus casei.

abstract：:Mycobacterium tuberculosis (Mtb) is the causative agent of the human disease Tuberculosis, and remains a worldwide health threat responsible for ∼1.7 million deaths annually. During infection, Mtb prevents acidification of the engulfing phagosome, thus blocking endocytic progression and eventually leading to stable re...

journal_title：Molecular microbiology

authors： Mann FM,VanderVen BC,Peters RJ

更新日期：2011-03-01 00:00:00

abstract：:We have cloned the gene encoding the TRK transporter of the soil yeast Schwanniomyces occidentalis and obtained the HAK1 trk1 delta and the hak1 delta TRK1 mutant strains. Analyses of the transport capacities of these mutants have shown that (i) the HAK1 and the TRK1 potassium transporters are the only transporters op...

journal_title：Molecular microbiology

authors： Bañuelos MA,Madrid R,Rodríguez-Navarro A

更新日期：2000-08-01 00:00:00

abstract：:ICEMlSym(R7A) of Mesorhizobium loti is an integrative and conjugative element (ICE) that confers the ability to form a nitrogen-fixing symbiosis with Lotus species. Horizontal transfer is activated by TraR and N-acyl-homoserine lactone (AHL), which can stimulate ICE excision in 100% of cells. However, in wild-type cu...

journal_title：Molecular microbiology

authors： Ramsay JP,Major AS,Komarovsky VM,Sullivan JT,Dy RL,Hynes MF,Salmond GP,Ronson CW

更新日期：2013-01-01 00:00:00

abstract：:The DNA encoding the zinc metalloprotease of Legionella pneumophila Philadelphia 1 has been isolated and expressed in Escherichia coli. This protein, which is 38,000 Daltons in size, possesses immunological and biochemical properties identical to those previously described for the purified L. pneumophila protease. Per...

journal_title：Molecular microbiology

authors： Quinn FD,Tompkins LS

更新日期：1989-06-01 00:00:00

abstract：:Most of the structural components of the flagellum of Salmonella typhimurium are exported through a flagellum-specific pathway, which is a member of the family of type III secretory pathways. The export apparatus for this process is poorly understood. A previous study has shown that two proteins, about 23 and 26 kDa i...

journal_title：Molecular microbiology

authors： Fan F,Ohnishi K,Francis NR,Macnab RM

更新日期：1997-12-01 00:00:00

abstract：:The Escherichia coli DNA glycosylase Mug excises 3,N(4)-ethenocytosines (epsilon C) and uracils from DNA, but its biological function is obscure. This is because epsilon C is not found in E. coli DNA, and uracil-DNA glycosylase (Ung), a distinct enzyme, is much more efficient at removing uracils from DNA than Mug. We ...

journal_title：Molecular microbiology

authors： Mokkapati SK,Fernández de Henestrosa AR,Bhagwat AS

更新日期：2001-09-01 00:00:00

abstract：:Natural genetic transformation in Streptococcus pneumoniae is controlled in part by a quorum-sensing system mediated by a peptide pheromone called competence-stimulating peptide (CSP), which acts to coordinate transient activation of genes required for competence. To characterize the transcriptional response and regul...

journal_title：Molecular microbiology

authors： Peterson SN,Sung CK,Cline R,Desai BV,Snesrud EC,Luo P,Walling J,Li H,Mintz M,Tsegaye G,Burr PC,Do Y,Ahn S,Gilbert J,Fleischmann RD,Morrison DA

更新日期：2004-02-01 00:00:00

abstract：:Pseudomonas aeruginosa PAO1, an opportunistic human pathogen, deploys several strategies to resist antibiotics. It uses multidrug efflux pumps, including the MexAB-OprM pump, for antibiotic resistance, and it also produces hydrogen sulfide (H2 S) that provides some defense against antibiotics. MexR functions as a tran...

journal_title：Molecular microbiology

authors： Xuan G,Lü C,Xu H,Chen Z,Li K,Liu H,Liu H,Xia Y,Xun L

更新日期：2020-12-01 00:00:00

abstract：:Protein phosphatases 2C are a family of conserved enzymes involved in many aspects of the cell biology. We reported that, in the yeast Saccharomyces cerevisiae, overexpression of the Ptc3p isoform resulted in increased lithium tolerance in the hypersensitive hal3 background. We have found that the tolerance induced by...

journal_title：Molecular microbiology

authors： Ruiz A,González A,García-Salcedo R,Ramos J,Ariño J

更新日期：2006-10-01 00:00:00

abstract：:The Bacillus subtilis GlnR transcription factor regulates gene expression in response to changes in nitrogen availability. Glutamine synthetase transmits the nitrogen regulatory signal to GlnR. The DNA-binding activity of GlnR is activated by a transient protein-protein interaction with feedback-inhibited glutamine sy...

journal_title：Molecular microbiology

authors： Wray LV Jr,Fisher SH

更新日期：2008-04-01 00:00:00

abstract：:The Escherichia coli treA gene encodes an osmotically inducible periplasmic trehalase. A strain carrying a treA-lacZ transcriptional fusion was constructed. The beta-galactosidase activity produced in this strain growing exponentially in a medium of high osmotic pressure was 10-fold higher than that produced in a medi...

journal_title：Molecular microbiology

authors： Repoila F,Gutierrez C

更新日期：1991-03-01 00:00:00

abstract：:The stalk is a universal structure of the large ribosomal subunit involved in the function of translation factors. The bacterial stalk is highly stable but its stability is notably reduced in eukaryotes, favouring a translation regulatory activity of this ribosomal domain, which has not been reported in prokaryotes. T...

journal_title：Molecular microbiology

authors： Santos C,Ballesta JP

更新日期：2005-10-01 00:00:00

abstract：:Accurate aminoacyl-tRNA synthesis is essential for correct translation of the genetic code in all organisms. Whereas many aspects of this process are conserved, others display a surprisingly high level of divergence from the canonical Escherichia coli model system. These differences are most pronounced in archaea wher...

journal_title：Molecular microbiology

authors： Praetorius-Ibba M,Ibba M

更新日期：2003-05-01 00:00:00

abstract：:The recent development of specific probes for lipid molecules has led to the discovery of lipid domains in bacterial membranes, that is, of membrane areas differing in lipid composition. A view of the membrane as a patchwork is replacing the assumption of lipid homogeneity inherent in the fluid mosaic model of Singer ...

journal_title：Molecular microbiology

authors： Matsumoto K,Kusaka J,Nishibori A,Hara H

更新日期：2006-09-01 00:00:00

abstract：:Pseudomonas syringae pv. tomato (Pst) strain DC3000 infects the model plants Arabidopsis thaliana and tomato, causing disease symptoms characterized by necrotic lesions surrounded by chlorosis. One mechanism used by Pst DC3000 to infect host plants is the type III protein secretion system, which is thought to deliver ...

journal_title：Molecular microbiology

authors： Zwiesler-Vollick J,Plovanich-Jones AE,Nomura K,Bandyopadhyay S,Joardar V,Kunkel BN,He SY

更新日期：2002-09-01 00:00:00

abstract：:The fibronectin binding protein, FnBPA, is a multifunctional microbial surface component recognizing adhesive matrix molecule (MSCRAMM) that promotes bacterial adherence to immobilized fibrinogen and elastin via the N-terminal A domain. The binding site for fibrinogen and elastin was localized to subdomains N2N3. A th...

journal_title：Molecular microbiology

authors： Keane FM,Loughman A,Valtulina V,Brennan M,Speziale P,Foster TJ

更新日期：2007-02-01 00:00:00

abstract：:Enhancement of enzyme thermostability by protein engineering gives us information about the thermostabilization mechanism as well as advantages for industrial use of enzymes. In this study, we enhanced the thermostability of endoglucanase EngB, one component of the cellulase complex (cellulosome) from Clostridium cell...

journal_title：Molecular microbiology

authors： Murashima K,Kosugi A,Doi RH

更新日期：2002-08-01 00:00:00

abstract：:The genes of nicotine dehydrogenase (NDH) were identified, cloned and sequenced from the catabolic plasmid pAO1 of Arthrobacter nicotinovorans. In immediate proximity to this gene cluster is the beginning of the 6-hydroxy-L-niotine oxidase (6-HLNO) gene. NDH is composed of three subunits (A, B and C) of M(r) 30,011, 1...

journal_title：Molecular microbiology

authors： Grether-Beck S,Igloi GL,Pust S,Schilz E,Decker K,Brandsch R

更新日期：1994-09-01 00:00:00

abstract：:A system where archaeal gene expression could be controlled by simple manipulation of growth conditions would enable the construction of conditional lethal mutants in essential genes, and permit the controlled overproduction of proteins in their native host. As tools for the genetic manipulation of Haloferax volcanii ...

journal_title：Molecular microbiology

authors： Large A,Stamme C,Lange C,Duan Z,Allers T,Soppa J,Lund PA

更新日期：2007-12-01 00:00:00

abstract：:A decline in our ability to successfully treat patients with malaria infections of the parasitic protozoan Plasmodium falciparum with cheap quinoline drugs has led to a huge escalation in morbidity and mortality in recent years. Many approaches have been taken, including classical genetics, reverse genetics and molecu...

journal_title：Molecular microbiology

authors： Duraisingh MT,Refour P

更新日期：2005-08-01 00:00:00

abstract：:Summary Successful malaria transmission from the mosquito vector to the mammalian host depends crucially on active sporozoite motility. Sporozoite locomotion and host cell invasion are driven by the parasite's own actin/myosin motor. A unique feature of this motor machinery is the presence of very short subpellicular ...

journal_title：Molecular microbiology

authors： Ganter M,Schüler H,Matuschewski K

更新日期：2009-12-01 00:00:00

abstract：:We identified several linked genes of a lactose regulon in Rhizobium meliloti. These were lacZ, the structural gene for beta-galactosidase; lacR, the lactose repressor gene; and two genes encoding proteins of unknown function, lacW and lacX. Insertion mutants in lacW and lacZ belonged to a single genetic complementati...

journal_title：Molecular microbiology

authors： Jelesko JG,Leigh JA

更新日期：1994-01-01 00:00:00

abstract：:Early diverged extant organisms, which may serve as convenient laboratory models to look for and study evolutionary ancient features of eukaryotic cell biology, are rare. The diplomonad Giardia intestinalis, a protozoan parasite known to cause diarrhoeal disease, has become an increasingly popular object of basic rese...

journal_title：Molecular microbiology

authors： Hehl AB,Marti M

更新日期：2004-07-01 00:00:00

abstract：:The bacterial flagellum contains a specialized secretion apparatus in its base that pumps certain protein subunits through the growing structure to their sites of installation beyond the membrane. A related apparatus functions in the injectisomes of gram-negative pathogens to export virulence factors into host cells. ...

journal_title：Molecular microbiology

authors： Erhardt M,Wheatley P,Kim EA,Hirano T,Zhang Y,Sarkar MK,Hughes KT,Blair DF

更新日期：2017-04-01 00:00:00

abstract：:The FNR protein of Escherichia coli is a regulatory protein that activates the transcription of its target genes in response to oxygen limitation. Site-directed mutagenesis was used to show that a 28-residue N-terminal segment containing three cysteines is essential for normal FNR function. The cysteine residue which ...

journal_title：Molecular microbiology

authors： Spiro S,Guest JR

更新日期：1988-11-01 00:00:00

abstract：:The ability of Salmonella enterica serovar Typhimurium to cause disease depends upon the co-ordinated expression of many genes located around the Salmonella chromosome. Specific pathogenicity loci, termed Salmonella pathogenicity islands, have been shown to be crucial for the invasion and survival of Salmonella within...

journal_title：Molecular microbiology

authors： Wilson RL,Libby SJ,Freet AM,Boddicker JD,Fahlen TF,Jones BD

更新日期：2001-01-01 00:00:00

abstract：:The Escherichia coli marRAB operon specifies two regulatory proteins, MarR (which represses) and MarA (which activates expression of the operon). The latter controls expression of multiple other chromosomal genes implicated in cell physiology, multiple drug resistance and virulence. Using randomly cloned E. coli DNA f...

journal_title：Molecular microbiology

authors： Domain F,Bina XR,Levy SB

更新日期：2007-10-01 00:00:00

abstract：:When Escherichia coli is exposed to redox-cycling drugs, its SoxR transcription factor is activated by oxidation of its [2Fe-2S] cluster. In aerobic cells these drugs generate superoxide, and because superoxide dismutase (SOD) is a member of the SoxRS regulon, superoxide was initially thought to be the activator of So...

journal_title：Molecular microbiology

authors： Gu M,Imlay JA

更新日期：2011-03-01 00:00:00

abstract：:A bioassay was developed to identify stimuli that promote the transcriptional induction of the algD operon for alginate biosynthesis in Pseudomonas aeruginosa. Strain PAO1 carried the algD promoter fused to a chloramphenicol acetyl-transferase cartridge (PalgD-cat), and > 50 compounds were tested for promoting chloram...

journal_title：Molecular microbiology

authors： Wood LF,Leech AJ,Ohman DE

更新日期：2006-10-01 00:00:00

abstract：:The exosporium is the outermost layer of the Bacillus anthracis spore. The predominant protein on the exosporium surface is BclA, a collagen-like glycoprotein. BclA is incorporated on the spore surface late in the B. anthracis sporulation pathway. A second collagen-like protein, BclB, has been shown to be surface-expo...

journal_title：Molecular microbiology

authors： Thompson BM,Stewart GC

更新日期：2008-10-01 00:00:00