abstract：:A full-length cDNA encoding the 24-kDa flagellar Ca(2+)-binding protein (FCaBP) of the Dm28c clone of Trypanosoma cruzi was cloned and characterized. Comparison of the deduced amino acid sequence with those of the FCaBPs of other T. cruzi strains revealed greater than 97% sequence conservation. FCaBP-like genes are fo...

journal_title：Experimental parasitology

authors： Maldonado RA,Linss J,Thomaz N,Olson CL,Engman DM,Goldenberg S

更新日期：1997-07-01 00:00:00

abstract：:We have identified congenic mouse strains that differ dramatically in resistance to infection with the murine malaria parasite Plasmodium yoelii 17X. After infection, BALB/c mice develop severe anemia and a high degree of parasitemia which sometimes results in death. The mtv-7 congenic strain BALB.D2.mlsa, however, de...

journal_title：Experimental parasitology

authors： Swardson CJ,Wassom DL,Avery AC

更新日期：1997-06-01 00:00:00

abstract：:Allelic variation in the Plasmodium falciparum merozoite surface protein 1 (MSP1) gene is expressed as an association of allelic types in variable blocks. In this study, a PCR strategy that can detect 24 different MSP1 association types was used to investigate allelic variation in the MSP1 gene. We identified 236 dist...

journal_title：Experimental parasitology

authors： Kaneko O,Kimura M,Kawamoto F,Ferreira MU,Tanabe K

更新日期：1997-05-01 00:00:00

abstract：:In murine Schistosoma mansoni infections, fewer adult worms develop in male than in female mice infected with the same number of cercariae. To evaluate a potential role for testosterone in this phenomenon, testosterone levels were manipulated in groups of CBA/J mice that were then infected and monitored for survival r...

journal_title：Experimental parasitology

authors： Nakazawa M,Fantappie MR,Freeman GL Jr,Eloi-Santos S,Olsen NJ,Kovacs WJ,Secor WE,Colley DG

更新日期：1997-03-01 00:00:00

abstract：:Murine malarial parasites have long been characterized by their requirement for either antibody-mediated immunity (AMI) or cell-mediated immunity (CMI) for suppression of acute parasitemia, with Plasmodium yoelii reportedly requiring AMI for suppression and P. chabaudi requiring CMI. To assess this characterization in...

journal_title：Experimental parasitology

authors： van der Heyde HC,Pepper B,Batchelder J,Cigel F,Weidanz WP

更新日期：1997-02-01 00:00:00

abstract：:The thymidine kinase gene of Herpes simplex type-1 virus was transfected into several Leishmania species to create drug-sensitive mutants. Expression of the thymidine kinase gene is not by itself harmful to Leishmania cells but it is capable of phosphorylating ganciclovir, a nucleoside analog, into a highly toxic prod...

journal_title：Experimental parasitology

authors： Muyombwe A,Olivier M,Ouellette M,Papadopoulou B

更新日期：1997-01-01 00:00:00

abstract：:We probed DNA from all trypanosomatid genera by slot blot hybridization with an oligonucleotide (SL3') complementary to a sequence of the Phytomonas spliced-leader or mini-exon RNA. The 19-nucleotide probe target site was previously shown to be highly conserved among a limited number of Phytomonas isolates, but diverg...

journal_title：Experimental parasitology

authors： Teixeira MM,Serrano MG,Nunes LR,Campaner M,Buck GA,Camargo EP

更新日期：1996-12-01 00:00:00

abstract：:Schistosoma mansoni muscle fibers contract in response to L-glutamate in a dose-dependent manner (10(-6)-10(-3) M). L-aspartate and D-aspartate are likewise effective in eliciting contraction of the fibers. Mammalian glutamate receptor agonists produce little or no contraction at concentrations as high as 1 mM. In add...

journal_title：Experimental parasitology

authors： Miller CL,Day TA,Bennett JL,Pax RA

更新日期：1996-12-01 00:00:00

abstract：:Immunity in mice infected with Toxoplasma gondii is dependent upon the ability to generate protective levels of the cytokine IFN-gamma. In this report, we present evidence that the attenuated vaccine strain, ts-4, induces the latter cytokine by both IL-12-dependent and -independent pathways. In contrast, strain ME49 a...

journal_title：Experimental parasitology

authors： Scharton-Kersten T,Caspar P,Sher A,Denkers EY

更新日期：1996-11-01 00:00:00

abstract：:Trypanosoma cruzi infection in humans and experimental animals often results in a chronic heart and gut inflammation and a dysfunction known as Chagas' disease. Previous studies have shown that the cellular infiltrate in the hearts of animals with chronic Chagas' disease consists mainly of CD8+ T cells. In this study,...

journal_title：Experimental parasitology

authors： Zhang L,Tarleton RL

更新日期：1996-11-01 00:00:00

abstract：:This review focuses on the role of IL-4 in the immune response which develops during infection with the trematode parasite Schistosoma mansoni. The emphasis is on our own recent studies in the mouse model. ...

journal_title：Experimental parasitology

authors： Pearce EJ,Vasconcelos JP,Brunet LR,Sabin EA

更新日期：1996-11-01 00:00:00

abstract：:A systematic study of the distribution of intracellular parasites in the organs and tissues of mice acutely infected (15 days) with the CL strain of Trypanosoma cruzi was performed. Almost all tissues and organs were parasitized with different intensities, including several epithelial cell types. In addition to striat...

journal_title：Experimental parasitology

authors： Lenzi HL,Oliveira DN,Lima MT,Gattass CR

更新日期：1996-10-01 00:00:00

abstract：:At least eight protein members of HSP70 and three of the HSP60 families have been identified in Trypanosoma cruzi; of these, five HSP70 isoforms and one HSP60 isoform were respectively induced by a 2-hr heat-shock treatment at 37 degrees C. Immunoelectronmicroscopy of epimastigote, spheromastigote, and metacyclic cell...

journal_title：Experimental parasitology

authors： Giambiagi-deMarval M,Souto-Padrón T,Rondinelli E

更新日期：1996-08-01 00:00:00

abstract：:Acquisition of iron from lactoferrin and transferrin by a parasitic protozoon Tritrichomonas foetus has been studied in vitro. Specific, time-dependent, and saturable binding of iodinated ligands to the outer membrane of T. foetus at 4 degrees C was demonstrated for 125I-labeled lactoferrin only. About 1.7 x 10(5) bin...

journal_title：Experimental parasitology

authors： Tachezy J,Kulda J,Bahníková I,Suchan P,Rázga J,Schrével J

更新日期：1996-07-01 00:00:00

abstract：:Culture forms of Leishmania (Leishmania) amazonensis (IFLA/BR/67/PH8) produce an extracellular enzyme that hydrolyzes sucrose molecules into their component monosaccharides. This is important because phlebotomine sand flies, the invertebrate hosts of Leishmania, ingest plant sap or aphid and coccid honeydew rich in su...

journal_title：Experimental parasitology

authors： Gontijo NF,Melo MN,Riani EB,Almeida-Silva S,Mares-Guia ML

更新日期：1996-06-01 00:00:00

abstract：:Schistosoma mansoni infection in humans and animals induces abnormal neurobehavioral responses following granuloma formation. In mice, granulomas in the liver are observed 8 weeks after infection, while after 15-20 weeks, the presence of eggs and granulomas in the brain has been reported. In this study, outbred CD-1 f...

journal_title：Experimental parasitology

authors： Fiore M,Moroni R,Alleva E,Aloe L

更新日期：1996-06-01 00:00:00

abstract：:Tc-85, an 85-kDa surface glycoprotein specific for the trypomastigote stage of Trypanosoma cruzi, has been implicated in the invasion of host cells by the parasite. Radioactive palmitic acid was incorporated into Tc-85 immunoprecipitated from the culture medium with the H1A10 monoclonal antibody, suggesting that shedd...

journal_title：Experimental parasitology

authors： Abuin G,Couto AS,de Lederkremer RM,Casal OL,Galli C,Colli W,Alves MJ

更新日期：1996-04-01 00:00:00

abstract：:Five-month-old beagle dogs were experimentally infected with amastigotes of Leishmania infantum and kept for 14 months. Infection course was monitored by clinical examination, serum protein variations, and levels of specific antibodies against Leishmania estimated by indirect immunofluorescence test and Western blotti...

journal_title：Experimental parasitology

authors： Carrera L,Fermín ML,Tesouro M,García P,Rollán E,González JL,Méndez S,Cuquerella M,Alunda JM

更新日期：1996-03-01 00:00:00

abstract：:The reactivity to overlapping decapeptides based on sequences of human band 3 protein was determined for 24 rabbit serum samples. A high percentage of the sera recognized sequences of amino acid 650-670, as well as several other sequences representing putative exofacial regions of the band 3 protein. When sera were us...

journal_title：Experimental parasitology

authors： Crandall I,Guthrie N,Sherman IW

更新日期：1996-01-01 00:00:00

abstract：:Rhoptries are involved in host cell invasion and rhoptry polypeptides, including the Babesia bigemina rhoptry-associated protein-1 (RAP-1), are targets for protective immune responses. Polyclonal antisera produced against isolated rhoptries is directed predominantly against RAP-1 and reacts with both the merozoite and...

journal_title：Experimental parasitology

authors： Vidotto O,McElwain TF,Machado RZ,Perryman LE,Suarez CE,Palmer GH

更新日期：1995-12-01 00:00:00

abstract：:The blood dwelling stages of schistosomes have acetylcholinesterase (AChE) and nicotinic-like acetylcholine receptors (nAChR) on their teguments. Both AChE and nAChR are concentrated on the dorsal surface of the adult male, a major surface for nutrient uptake for the worm pair. Exposure of tegumental AChE and nAChR to...

journal_title：Experimental parasitology

authors： Camacho M,Agnew A

更新日期：1995-12-01 00:00:00

abstract：:The yellow fever mosquito, Aedes aegypti, expresses a gene, Apyrase (Apy), that encodes an ATP-diphosphohydrolase. The product of this gene is a secreted enzyme that facilitates hematophagy by preventing platelet aggregation in the host. Apy gene expression is limited to the cells of the distal-lateral and medial lobe...

journal_title：Experimental parasitology

authors： Smartt CT,Kim AP,Grossman GL,James AA

更新日期：1995-11-01 00:00:00

abstract：:There is evidence which suggests that malaria sporozoites recognize mosquito salivary glands by specific receptor-ligand interactions. We are interested in identifying the putative salivary gland receptor(s) for sporozoite invasion. We used an in vivo bioassay for sporozoite invasion of salivary glands. In this assay,...

journal_title：Experimental parasitology

authors： Barreau C,Touray M,Pimenta PF,Miller LH,Vernick KD

更新日期：1995-11-01 00:00:00

abstract：:Stable RNAs have regions of primary sequence that are nearly identical in every member of the Plasmodium genus and not found in the host or in other common pathogens. Several "genus-conserved" sequences, which flank hypervariable regions, were identified within the small subunit ribosomal RNA of Plasmodium species. Pr...

journal_title：Experimental parasitology

authors： Li J,Wirtz RA,McConkey GA,Sattabongkot J,Waters AP,Rogers MJ,McCutchan TF

更新日期：1995-09-01 00:00:00

abstract：:Twenty-one Trypanosoma cruzi stocks isolated from Triatoma infestans and humans of the Chilean Andean highlands were studied at the genotypic level by schizodeme and molecular karyotype analyses, which allowed a clear distinction of the parasites from those hosts. A phenotypical characterization was performed by prote...

journal_title：Experimental parasitology

authors： Gonzalez J,Muñoz S,Ortiz S,Anacona D,Salgado S,Galleguillos M,Neira I,Sagua H,Solari A

更新日期：1995-08-01 00:00:00

abstract：:Members of a serologically cross-reacting family of proteins including Ag332 and Pf11.1, megadalton proteins of schizont-infected red blood cells, and gametocytes, respectively, and Pf155-RESA, a 155-kDa protein of ring-infected red blood cells, have been reported to share amino acid repeat sequences. These repeats ar...

journal_title：Experimental parasitology

authors： Barnes DA,Wollish W,Nelson RG,Leech JH,Petersen C

更新日期：1995-08-01 00:00:00

abstract：:The effect of a synthetic cecropin-like peptide, Shiva-3, on in vitro ookinete development and on the early sporogonic stages of Plasmodium berghei in the midgut of Anopheles albimanus was investigated. Peptide concentrations of 75 and 100 microM were effective (P < 0.05) in reducing ookinete production and the number...

journal_title：Experimental parasitology

authors： Rodriguez MC,Zamudio F,Torres JA,Gonzalez-Ceron L,Possani LD,Rodriguez MH

更新日期：1995-06-01 00:00:00

abstract：:Six chimpanzees (Pan troglodytes) and six mangabey monkeys (Cercocebus atys) were inoculated with Onchocerca volvulus third-stage larvae (L3) of West African origin. Two chimpanzees each received 200, 300, or 400 L3, while three mangabeys each received either 50 or 250 L3. All six chimpanzees became microfilaria posit...

journal_title：Experimental parasitology

authors： Eberhard ML,Dickerson JW,Tsang VC,Walker EM,Ottesen EA,Chandrashekar R,Weil GJ,Trpis M,Strobert E,Constantinidis I

更新日期：1995-05-01 00:00:00

abstract：:The in vitro growth of Plasmodium falciparum was reduced by 35 and 43% through high concentrations (5 mmole/liter) of magnesium in RPMI medium and magnesium-free medium, respectively, after 48 hr, whereas no significant inhibition could be observed under these conditions after 24 hr cultivation in the respective mediu...

journal_title：Experimental parasitology

authors： Hess FI,Kilian A,Söllner W,Nothdurft HD,Pröll S,Löscher T

更新日期：1995-03-01 00:00:00

abstract：:A murine model system was developed to study the induction and mechanism of protective immunity to L3 of Strongyloides stercoralis. L3 were implanted in BALB/cByJ mice in diffusion chambers constructed with 0.1- or 2.0-microns-pore-size membranes. Parasites survived equally well regardless or membrane type for 7 days,...

journal_title：Experimental parasitology

authors： Abraham D,Rotman HL,Haberstroh HF,Yutanawiboonchai W,Brigandi RA,Leon O,Nolan TJ,Schad GA

更新日期：1995-03-01 00:00:00

abstract：:Partial nucleotide sequences of large subunit ribosomal RNA (LSU rRNA) coding genes of Theileria parva and T. taurotragi were determined. Alignment of the T. parva and T. taurotragi sequences revealed species-specific regions within the LSU rRNA genes. A major species-variable region, located between the first and sec...

journal_title：Experimental parasitology

authors： Bishop R,Allsopp B,Spooner P,Sohanpal B,Morzaria S,Gobright E

更新日期：1995-02-01 00:00:00

abstract：:Toxoplasma gondii and the related Apicomplexan protozoan pathogens, Plasmodium, Cryptosporidium, and Eimeria, are obligate intracellular parasites which cause severe disease in their hosts. The recent development of transient transfection of Toxoplasma permits the development of strategies utilizing "reverse genetics"...

journal_title：Experimental parasitology

authors： Kim K,Boothroyd JC

更新日期：1995-02-01 00:00:00

abstract：:The diagnostic potential of recombinant E/S antigens of the lymphatic filaria Brugia malayi was investigated by Western blot. A cDNA expression library was constructed using B. malayi male adult worm mRNA, and E/S recombinants were identified with a rabbit antiserum raised against E/S products collected in vitro from ...

journal_title：Experimental parasitology

authors： Kumari S,Lillibridge CD,Bakeer M,Lowrie RC Jr,Jayaraman K,Philipp MT

更新日期：1994-12-01 00:00:00

abstract：:The transport of the megadalton protein Pf332 was studied during the asexual bloodstage development of Plasmodium falciparum. Four mouse monoclonal antibodies, produced against a recombinant polypeptide derived from the Pf332 protein, were used to analyze the kinetics of synthesis, the subcellular location, and transp...

journal_title：Experimental parasitology

authors： Hinterberg K,Scherf A,Gysin J,Toyoshima T,Aikawa M,Mazie JC,da Silva LP,Mattei D

更新日期：1994-11-01 00:00:00

abstract：:Indirect immunocytochemistry, in conjunction with confocal scanning laser microscopy and electron-microscopic immunogold labeling, has been used to localize neuropeptide and 5-hydroxytryptamine (5-HT) immunoreactivities (IRs) in the plerocercoid (scolex and surrounding blastocyst) of the trypanorhynch tapeworm, Grillo...

journal_title：Experimental parasitology

authors： Halton DW,Maule AG,Brennan GP,Shaw C,Stoitsova SR,Johnston CF

更新日期：1994-11-01 00:00:00

abstract：:As an approach to better understand the molecular mechanisms underlying the differential cellular response to larval Schistosoma mansoni observed in Biomphalaria glabrata, we have analyzed hemocyte membrane-associated polypeptides from inbred susceptible and resistant strains of B. glabrata. A combination of technique...

journal_title：Experimental parasitology

authors： Coustau C,Yoshino TP

更新日期：1994-08-01 00:00:00

abstract：:The blood and spleen lymphocyte subsets and parasite burdens in blood, lungs, and brain were determined serially in C57B1/6 mice infected with an avirulent strain of Toxoplasma gondii. Five mice were sacrificed at various time points after infection; Thy1-2+, Ly5+, CD4+, CD8+, and Ly6c+ lymphocyte subsets and macropha...

journal_title：Experimental parasitology

authors： Carrof B,Levacher-Clergeot M,Chau F,Pocidalo JJ,Derouin F

更新日期：1994-06-01 00:00:00

abstract：:Monoclonal antibodies (mAb) directed against Schistosoma mansoni tropomyosin isoform, SMTM (Xu et al. Experimental Parasitology 69, 373-392, 1989), were used to test for cross-reactivity with Biomphalaria glabrata antigens. One mAb (1F10) recognized antigens of 39, 41, and 80 kDa in a snail head/foot antigen preparati...

journal_title：Experimental parasitology

authors： Weston D,Allen B,Thakur A,LoVerde PT,Kemp WM

更新日期：1994-05-01 00:00:00

abstract：:Feeding behavior and some antihemostatic properties of the saliva of Rhodnius prolixus infected with Trypanosoma rangeli were studied and compared with those of uninfected controls. Insects with salivary gland infection pierced host skin more often and drew less blood and at a lower rate than controls when feeding on ...

journal_title：Experimental parasitology

authors： Garcia ES,Mello CB,Azambuja P,Ribeiro JM

更新日期：1994-05-01 00:00:00

abstract：:There has been some evidence from several parasite systems that proteinases might have potential as protective antigens against parasitic infection. A cysteine proteinase complex identified in the regurgitant of adult F. hepatica was examined in this context. The thiol-cathepsin-related proteinases of M(r) 28,000 were...

journal_title：Experimental parasitology

authors： Wijffels GL,Salvatore L,Dosen M,Waddington J,Wilson L,Thompson C,Campbell N,Sexton J,Wicker J,Bowen F

更新日期：1994-03-01 00:00:00