Abstract：
:There is evidence which suggests that malaria sporozoites recognize mosquito salivary glands by specific receptor-ligand interactions. We are interested in identifying the putative salivary gland receptor(s) for sporozoite invasion. We used an in vivo bioassay for sporozoite invasion of salivary glands. In this assay, purified sporozoites from mature oocytes of Plasmodium gallinaceum were injected into Aedes aegypti mosquitoes and salivary glands were dissected at different time points after injection. One half of the maximum invasion of salivary glands by sporozoites occurred by 6 hr, and salivary gland sporozoite load did not increase further after 24 hr postinjection. This assay was used to determine the effect of experimental treatments with antibodies and lectins at 24 hr postinjection. We raised a rabbit polyclonal antiserum against female Ae. aegypti salivary glands which recognized tissue-specific determinants in the basal lamina of salivary glands. Purified IgG antibody fraction of the immune serum blocked sporozoite invasion in vivo. We tested a panel of 19 lectins and found 7 which bound to salivary glands. Of these 7, succinylated wheat germ agglutinin and wheat germ agglutinin completely blocked sporozoite invasion; Pisum sativum agglutinin and soybean agglutinin partially blocked; and concanavalin A, Dolichos biflorus agglutinin, and Phaseolus vulgaris erythroagglutinin did not block. Our results suggest that sporozoites interact with glycosylated salivary gland surface molecules which serve as receptors for invasion, and which may be in the salivary gland basal lamina. Because the putative sporozoite receptors contain immunogenic determinants, it is feasible to identify them by an immunological strategy.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Barreau C,Touray M,Pimenta PF,Miller LH,Vernick KDdoi
10.1006/expr.1995.1124subject
Has Abstractpub_date
1995-11-01 00:00:00pages
332-43issue
3eissn
0014-4894issn
1090-2449pii
S0014-4894(85)71124-1journal_volume
81pub_type
杂志文章abstract：:Coccidiosis is a major parasitic disease of poultry industry and an ideal vaccine should induce long-lasting cross-species protective immunity. Broiler chickens (Cobb 500) were inoculated with single, double or triple injections of a synthetic peptide (derived from sequences of Eimeria acervulina and Eimeria tenella a...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2005.03.026
更新日期：2005-08-01 00:00:00
abstract：:In March 2010, a 35 year-old HIV/AIDS female patient was admitted to hospital to start treatment with Highly Active Antiretroviral Therapy (HAART) since during a routine control a dramatic decrease in the CD4(+) levels was detected. At this stage, a nasal swab from each nostril was collected from the patient to includ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2014.02.014
更新日期：2014-11-01 00:00:00
abstract：:Trypanosoma cruzi infection in humans and experimental animals often results in a chronic heart and gut inflammation and a dysfunction known as Chagas' disease. Previous studies have shown that the cellular infiltrate in the hearts of animals with chronic Chagas' disease consists mainly of CD8+ T cells. In this study,...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1996.0106
更新日期：1996-11-01 00:00:00
abstract：:A molecular study was carried out to incriminate sand fly vectors of cutaneous leishmaniasis (CL) in rural areas of Sarakhs district, Khorassane-Razavi Province, northeastern Iran, in 2011. Sand flies of Sergentomyia with three species and Phlebotomus with six species respectively comprised 73.3% and 26.7% of the spec...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2013.07.020
更新日期：2013-10-01 00:00:00
abstract：:This paper describes the in vitro multiplication process of Babesia bigemina sporokinetes in a cell line (IDE8) from Ixodes scapularis ticks. The inoculum was obtained from hemolymph of engorged females of Rhipicephalus (Boophilus) microplus ticks naturally infected with B. bigemina. These ticks had been fed on calves...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2009.03.011
更新日期：2009-07-01 00:00:00
abstract：:The blood dwelling stages of schistosomes have acetylcholinesterase (AChE) and nicotinic-like acetylcholine receptors (nAChR) on their teguments. Both AChE and nAChR are concentrated on the dorsal surface of the adult male, a major surface for nutrient uptake for the worm pair. Exposure of tegumental AChE and nAChR to...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1995.1152
更新日期：1995-12-01 00:00:00
abstract：:How the avermectins cause the elimination of gastrointestinal nematodes from host animals has not yet been clearly identified. Using visual and radiometric parameters to measure oral ingestion in Haemonchus contortus, we showed that ivermectin (IVM) rapidly inhibited ingestion at concentrations > or = 10(-10) M. Motil...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1993.1064
更新日期：1993-08-01 00:00:00
abstract：:Entomopathogenic nematode Heterorhabditis bacteriophora Az29 and Az36 isolates with different virulence against Popillia unipuncta and soil survival time were isolated from the Azorean archipelago (Portugal) and used for the study. RAPD analysis revealed a very low-level of genetic diversity (GD(axenic Az36 isolate)(a...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2011.12.001
更新日期：2012-02-01 00:00:00
abstract：:Standardization of the methods for extraction of DNA from sand flies is essential for obtaining high efficiency during subsequent molecular analyses, such as the new sequencing methods. Information obtained using these methods may contribute substantially to taxonomic, evolutionary, and eco-epidemiological studies. Th...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2017.04.010
更新日期：2017-06-01 00:00:00
abstract：:Toxoplasma gondii is an intra-cellular parasite that infects humans through vertical and horizontal transmission. The cysts remain dormant in the brain of infected humans and can reactivate in immunocompromised hosts resulting in acute toxoplasmic encephalitis which may be fatal. We determined the onset and progressio...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2011.10.004
更新日期：2012-01-01 00:00:00
abstract：:The present study aimed to evaluate the acaricidal efficacy of fluazuron (2.5 mg/kg), administered as a pour-on, in comparison to an injectable formulation containing fluazuron (1.6 mg/kg) + ivermectin (0.63 mg/kg), against Rhipicephalus (Boophilus) microplus in naturally and experimentally infested cattle. Two studie...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2015.02.004
更新日期：2015-06-01 00:00:00
abstract：:Cardiac beta-adrenergic receptors' function was studied in the acute phase of Chagas' disease in mice reinfected with Trypanosoma cruzi Tulahuen strain (Tul) and with parasites isolated from an infected patient (SGO-Z12). Genetic characterization of SGO-Z12 isolates demonstrated that it belongs to the zimodeme Z12, on...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/s0014-4894(03)00096-1
更新日期：2003-03-01 00:00:00
abstract：:Monoclonal antibodies (mAb) directed against Schistosoma mansoni tropomyosin isoform, SMTM (Xu et al. Experimental Parasitology 69, 373-392, 1989), were used to test for cross-reactivity with Biomphalaria glabrata antigens. One mAb (1F10) recognized antigens of 39, 41, and 80 kDa in a snail head/foot antigen preparati...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1994.1028
更新日期：1994-05-01 00:00:00
abstract：:Rosetting is a parasite adhesion phenotype associated with severe malaria in African children. Why parasites form rosettes is unknown, although enhanced invasion or immune evasion have been suggested as possible functions. Previous work showed that rosetting does not enhance parasite invasion under standard in vitro c...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2005.11.007
更新日期：2006-04-01 00:00:00
abstract：:EhRabB is an Entamoeba histolytica protein involved in phagocytosis. However, proteins that regulate the EhRabB activity are unknown. Here, we report the identification of a putative G protein-coupled receptor of E. histolytica (EhGPCR-1) that binds to EhRabB. By two-hybrid screening, we found a 372-bp cDNA fragment t...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2005.02.014
更新日期：2005-07-01 00:00:00
abstract：:Three membrane thermotropic transitions at 8, 20, and 40 degrees C have been detected in human red blood cells (RBC) by using spin-labeled stearic acids. Red blood cells infected in vitro by Plasmodium falciparum showed the disappearance of the 8 degrees C transition and a lowering of the 40 degrees C transition to 32...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(89)90073-8
更新日期：1989-10-01 00:00:00
abstract：:Under a variety of stress conditions, Leishmania species display some morphological and biochemical features characteristic of mammalian programmed cell death or necrosis. Nitroheteroaryl-1,3,4-thiadiazoles induce cell death in Leishmania major (L. major). Putative mechanisms of action of these compounds were investig...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2012.06.002
更新日期：2012-10-01 00:00:00
abstract：:Inbred strains of mice differ in their susceptibility and resistance to challenge infections with Nematospiroides dubius. In our studies, F1 hybrid mice from resistant SJL and susceptible CBA parents were resistant to N. dubius challenge infections. Only 22% of backcrosses to SJL were susceptible while backcrosses to ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(88)90003-3
更新日期：1988-10-01 00:00:00
abstract：:The aim of this study was to evaluate the activity of purinergic enzymes in lymphocytes and cardiac tissue of mice experimentally infected by Trypanosoma cruzi. Twelve female mice were used, divided into two groups (n = 6): uninfected and infected. On day 12 post-infection (PI), the animals were anesthetized and after...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2017.02.002
更新日期：2017-04-01 00:00:00
abstract：:Typically, antimicrobial peptides (AMPs) are short positive charged peptides serving a key role in innate immunity as well as antimicrobial activity. Discovering novel therapeutic agents is considered as an undeniable demand due to increasing microbial species with antibiotic resistance. In this direction, the unique ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2019.107823
更新日期：2020-02-01 00:00:00
abstract：:Previous studies showed that Echinococcus granulosus infection reduces allergic airway inflammation in experimentally infected hosts and the cystic fluid of E. granulosus is known to activate regulatory T (CD4+CD25+Foxp3+T, Treg) cells. To evaluate the effects of cystic fluid of E. granulosus on allergic airway inflam...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2019.02.003
更新日期：2019-03-01 00:00:00
abstract：:Immunity in mice infected with Toxoplasma gondii is dependent upon the ability to generate protective levels of the cytokine IFN-gamma. In this report, we present evidence that the attenuated vaccine strain, ts-4, induces the latter cytokine by both IL-12-dependent and -independent pathways. In contrast, strain ME49 a...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1996.0096
更新日期：1996-11-01 00:00:00
abstract：:This study investigated the genetic characteristics of Toxoplasma gondii samples collected from 62 patients with toxoplasmosis in Sao Paulo State, Brazil. DNA samples were isolated from blood, cerebrospinal fluid and amniotic fluids of 25 patients with cerebral toxoplasmosis and AIDS, two patients with acute toxoplasm...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2011.06.002
更新日期：2011-10-01 00:00:00
abstract：:The experiments described here report on the correlation between the ex vivo diffusion of different benzimidazole (BZD) anthelmintics into the cestode parasite Moniezia benedeni, and their octanol-water partition coefficients (P.C.). The characterisation of the drug diffusion process into target parasites is relevant ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/s0014-4894(03)00060-2
更新日期：2003-01-01 00:00:00
abstract：:The swimming behavior of Poecilia vivipara was evaluated using an image analysis system comparing laboratory-reared uninfected fish before and after experimental infection with different intensities of cercariae of the trematode Acanthocollaritrema umbilicatum. Two experiments were performed, each with 30 fish which w...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2010.10.018
更新日期：2011-02-01 00:00:00
abstract：:Excystation of Giardia lamblia entails differentiation of dormant cysts into parasitic trophozoites. Despite its importance for infection, this transformation is not understood at the cellular or molecular levels. In these studies, we report that excystation entails detection of environmental stimuli across the tough ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1998.4246
更新日期：1998-03-01 00:00:00
abstract：:A rodent model of malaria, Plasmodium berghei was used to assess the antimalarial potential of dinitroaniline herbicides. Trifluralin, pendimethalin, oryzalin, and benfluralin were all active against P. berghei in vitro at, or close to, submicromolar concentrations, with a rank order of potency similar to that against...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/s0014-4894(02)00016-4
更新日期：2002-03-01 00:00:00
abstract：:Twenty-one Trypanosoma cruzi stocks isolated from Triatoma infestans and humans of the Chilean Andean highlands were studied at the genotypic level by schizodeme and molecular karyotype analyses, which allowed a clear distinction of the parasites from those hosts. A phenotypical characterization was performed by prote...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1995.1100
更新日期：1995-08-01 00:00:00
abstract：:Improved methods for efficient excystation of Eimeria should be developed and standardized for future Eimeria-related studies. Here, the effects of different glass bead sizes (0.5, 1, 2, and 2.5 mm), and various vortex speeds (1000, 2000, and 3000 rpm) and durations (30 s, 1, 3, and 5 min) have been examined for Eimer...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2014.01.001
更新日期：2014-03-01 00:00:00
abstract：:Hemocytes derived from a strain (13-16-R1) of Biomphalaria glabrata resistant to Schistosoma mansoni were significantly more likely to bind untreated latex beads than hemocytes from the schistosome-susceptible M line strain. Beads preincubated in 13-16-R1 plasma were more readily bound by both 13-16-R1 and M line hemo...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(92)90182-a
更新日期：1992-09-01 00:00:00
