Abstract：
:This study investigated the genetic characteristics of Toxoplasma gondii samples collected from 62 patients with toxoplasmosis in Sao Paulo State, Brazil. DNA samples were isolated from blood, cerebrospinal fluid and amniotic fluids of 25 patients with cerebral toxoplasmosis and AIDS, two patients with acute toxoplasmosis, 12 patients with ocular toxoplasmosis, six newborns with congenital toxoplasmosis and 17 pregnant women with acute infection. Diagnosis of toxoplasmosis was based in clinical, radiological and laboratory features. Genotyping was performed using multilocus PCR-RFLP genetic markers including SAG1, SAG2, 5'- and 3'-SAG2, alt.SAG2, SAG3, BTUB, GRA6, C22-8, c29-2, L358, PK1 and Apico. Among the 62 clinical samples, 20 (32%) were successfully genotyped at eight or more genetic loci and were grouped to three distinct genotypes. Eighteen samples belonged to ToxoDB Genotype #65 and the other two samples were identified as ToxoDB Genotypes #6 and #71, respectively (http://toxodb.org/toxo/). Patients presenting Genotypes #6 and #71 had severe and atypical cerebral toxoplasmosis, characterized by diffuse encephalitis without extensive brain lesions. These results indicate that T. gondii Genotype #65 may have a high frequency in causing human toxoplasmosis in Sao Paulo State, Brazil. This unusual finding highlights the need to investigate the possible association of parasite genotypes with human toxoplasmosis.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Ferreira IM,Vidal JE,de Mattos Cde C,de Mattos LC,Qu D,Su C,Pereira-Chioccola VLdoi
10.1016/j.exppara.2011.06.002subject
Has Abstractpub_date
2011-10-01 00:00:00pages
190-5issue
2eissn
0014-4894issn
1090-2449pii
S0014-4894(11)00176-7journal_volume
129pub_type
杂志文章abstract：:The sheep tapeworm (Moniezia expansa) and its host Ovis aries were analyzed by inductively coupled plasma optical emission spectrometry (ICP-OES) for their copper, iron, manganese, zinc and lead levels. Element concentrations in cestode parasites were compared to those in various organs (liver, kidney, and muscle) of ...
journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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doi：10.1006/expr.1993.1064
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
pub_type： 杂志文章
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2009.10.011
更新日期：2010-09-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
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更新日期：2009-03-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2006.04.010
更新日期：2006-12-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2015.08.009
更新日期：2015-12-01 00:00:00
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1994.1110
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1995.1023
更新日期：1995-03-01 00:00:00
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journal_title：Experimental parasitology
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