Abstract：
:Trichomonas vaginalis is a human urogenital pathogen that causes trichomoniasis, the most common nonviral, parasitic sexually transmitted infection in the world. At present, little is known regarding the degree of strain variability of T. vaginalis. A classification method for T. vaginalis strains would be a useful tool in the study of the epidemiology, drug resistance, pathogenesis and transmission of T. vaginalis. Eight different types of actin genes have been identified by PCR-RFLP in T. vaginalis; the purpose of this study is to determine the genotypes of this parasite in Karaj city, Iran. Forty-five clinical T. vaginalis isolates from vaginal secretions and urine sediment were collected from Karaj city from 2012 through 2014. DNA was extracted and the actin gene was amplified by nested-PCR; all samples were positive. To determine the genetic differences, sequencing on seven samples was conducted. Then, all PCR products were digested with HindII, MseI, and RsaI restriction enzymes. Of 45 isolates, 23 samples (51.1%) were of actin genotype G, 11 samples (24.4%) of genotype E, six samples (13.3%) of genotype H, three samples (6.6%) of genotype I, and two samples (4.4%) were mixed genotypes of G and E. Genetic diversity of T. vaginalis isolates is notable. The actin genotype G may be the dominant genotype in Karaj city, Iran.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Momeni Z,Sadraei J,Kazemi B,Dalimi Adoi
10.1016/j.exppara.2015.10.011subject
Has Abstractpub_date
2015-12-01 00:00:00pages
259-63eissn
0014-4894issn
1090-2449pii
S0014-4894(15)30058-8journal_volume
159pub_type
杂志文章abstract：:Baccharis dracunculifolia DC (common name "alecrim-do-campo" in Brazil) is a plant with widespread distribution in South America that is the botanical origin of green propolis. The aim of this study was to evaluate the chemical composition and acaricidal activity of the essential oil of B. dracunculifolia and its cons...
journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
pub_type： 杂志文章
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1996.0036
更新日期：1996-04-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
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更新日期：2002-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.exppara.2007.01.019
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Experimental parasitology
pub_type： 杂志文章
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2005.02.014
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journal_title：Experimental parasitology
pub_type： 杂志文章
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journal_title：Experimental parasitology
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Experimental parasitology
pub_type： 杂志文章
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2008.08.004
更新日期：2008-12-01 00:00:00
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
pub_type： 杂志文章
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更新日期：2008-07-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2004.06.006
更新日期：2004-07-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2006.03.007
更新日期：2006-11-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1993.1066
更新日期：1993-08-01 00:00:00
abstract：:One difficulty facing post-genomic analyses of schistosomes is the limited data on sites of expression of many gene products expressed by the parasites in their hosts. The potential for use of laser microdissection microscopy as a preparative technique for transcriptional and proteomic profiling is reviewed. This tech...
journal_title：Experimental parasitology
pub_type： 杂志文章,评审
doi：10.1016/j.exppara.2007.06.004
更新日期：2007-11-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(84)90056-0
更新日期：1984-02-01 00:00:00
abstract：:Leishmania is an obligate intracellular protozoan parasite that infects cells of the reticulo-endothelial system. Host defences against Leishmania include fever and oxidant production, and the parasite has developed a number of defence mechanisms to neutralize the host response. The Leishmania donovani A2 family of pr...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2012.06.001
更新日期：2012-10-01 00:00:00
