Schistosoma mansoni: developmental arrest of miracidia treated with histone deacetylase inhibitors.

abstract：:The contribution of mononuclear phagocytes to host resistance against acute Chagas disease has been studied in vivo in a rat model inoculated with Trypanosoma cruzi, Y strain. Acute T. cruzi infection triggered a dramatic increase (937%) in peripheral blood monocyte number at day 12 of infection. At this point, histol...

journal_title：Experimental parasitology

authors： Melo RC,Machado CR

更新日期：2001-01-01 00:00:00

abstract：:BmSI-7 and BmSI-6, two Boophilus microplus subtilisin inhibitors (BmSI) were purified and characterized from eggs. The inhibitors isolated by classical purification methods presented molecular masses of 7408 and 7271Da, respectively, by MALDI-TOF-MS. Both BmSI-7 and BmSI-6 inhibited neutrophil elastase (K(i) 0.4 and 0...

journal_title：Experimental parasitology

authors： Sasaki SD,de Lima CA,Lovato DV,Juliano MA,Torquato RJ,Tanaka AS

更新日期：2008-02-01 00:00:00

abstract：:Though parasite cyclophilins are promising new drug targets, Eimeria tenella cyclophilins have not been characterized yet. Here, we describe an 89kDa cyclophilin, designated EtCYP89. It is expressed throughout the developmental cycle of E. tenella, both in the intracellular stages in chicken and in extracellular sporu...

journal_title：Experimental parasitology

authors： Hosse RJ,Krücken J,Bierbaum S,Greif G,Wunderlich F

更新日期：2008-02-01 00:00:00

abstract：:In this report, an immunodominant antigen called BgIRA from Babesia gibsoni is identified and described. A highly repetitive antigen was screened from a cDNA library. The genomic BgIRA gene exists as single cope gene and contains 10 introns. BgIRA plays a dominant role in the immune response in dogs infected with B. g...

journal_title：Experimental parasitology

authors： Jia H,Terkawi MA,Aboge GO,Goo YK,Zhou J,Lee EG,Nishikawa Y,Igarashi I,Fujisaki K,Xuan X

更新日期：2008-01-01 00:00:00

abstract：:Toxoplasma gondii is responsible for fetopathy in farm animals and humans and severe disease in immunocompromised individuals (i.e. AIDS patients). Effective vaccines, inducing protective and long-lasting immunity to this global parasite, are still desired. In the work, we evaluated the immunogenic and immunoprotectiv...

journal_title：Experimental parasitology

authors： Gatkowska J,Gasior A,Kur J,Dlugonska H

更新日期：2008-02-01 00:00:00

abstract：:Nitric oxide (NO), derived from catalysis of inducible NO synthase (iNOS), limits malaria parasite growth in mammals. Transforming growth factor (TGF)-beta1 suppresses iNOS in cells in vitro as well as in vivo in mice, but paradoxically severe malaria in humans is associated with low levels of TGF-beta1. We hypothesiz...

journal_title：Experimental parasitology

authors： Luckhart S,Lieber MJ,Singh N,Zamora R,Vodovotz Y

更新日期：2008-02-01 00:00:00

abstract：:One difficulty facing post-genomic analyses of schistosomes is the limited data on sites of expression of many gene products expressed by the parasites in their hosts. The potential for use of laser microdissection microscopy as a preparative technique for transcriptional and proteomic profiling is reviewed. This tech...

journal_title：Experimental parasitology

authors： Jones MK,Higgins T,Stenzel DJ,Gobert GN

更新日期：2007-11-01 00:00:00

abstract：:Plasmodium falciparum is usually asynchronous during in vitro culture. Highly synchronized cultures of P. falciparum are routinely used in malaria research. Here, we describe a simple synchronization procedure for P. falciparum asexual erythrocytic culture, which involves storage at 4°C for 8-24 h followed by routine ...

journal_title：Experimental parasitology

authors： Yuan L,Hao M,Wu L,Zhao Z,Rosenthal BM,Li X,He Y,Sun L,Feng G,Xiang Z,Cui L,Yang Z

更新日期：2014-05-01 00:00:00

abstract：:An increased level of plasma corticosterone is one manifestation of severe environmental or physiologic stress. The stress response mediated by the hypothalamic-pituitary-adrenal axis is already known to suppress immunoglobulin production and to impair immune function, but there are few studies relating stress and pla...

journal_title：Experimental parasitology

authors： Santos CD,Prado JC Jr,Toldo MP,Levy AM,Franci CR,Caldeira JC

更新日期：2007-12-01 00:00:00

abstract：:Polymerase chain reaction amplification of several polymorphic genes has been used to study the population biology of Plasmodium falciparum. S antigen is particularly suitable for such studies, but difficulties in the amplification of this gene have precluded its use. Here we describe a simple method for the amplifica...

journal_title：Experimental parasitology

authors： Kyes S,Craig AG,Marsh K,Newbold CI

更新日期：1993-12-01 00:00:00

abstract：:Isocitrate lyase (EC 4.1.3.1) from mixed larval populations of Caenorhabditis elegans was stabilized in crude extracts by centrifugation over a 0.2-0.6 M sucrose gradient for 2.5 hr in a vertical rotor (VTi 50) at 210,000g. The peak fractions from this sucrose gradient showed a half-life of 33 hr at 30 C and 225 hr at...

journal_title：Experimental parasitology

authors： Jameel S,McFadden BA

更新日期：1985-06-01 00:00:00

abstract：:Acanthamoeba culbertsoni trophozoites, previously isolated from a human keratitis case with severe intraocular damage, were maintained in axenic culture. Co-incubation of amoebae with MDCK cell monolayers demonstrated an apparent preference of the amoebae to introduce themselves between the cells. The trophozoites app...

journal_title：Experimental parasitology

authors： González-Robles A,Omaña-Molina M,Salazar-Villatoro L,Flores-Maldonado C,Lorenzo-Morales J,Reyes-Batlle M,Arnalich-Montiel F,Martínez-Palomo A

更新日期：2017-12-01 00:00:00

abstract：:Parasite resistance to antimalarial drugs, particularly chloroquine, is the most disturbing problem of malaria chemotherapy. There is evidence that the codon 86Tyr polymorphism of the Pfmdr1 gene is associated with chloroquine resistance in West African Plasmodium falciparum. The association of this and four other cod...

journal_title：Experimental parasitology

authors： Póvoa MM,Adagu IS,Oliveira SG,Machado RL,Miles MA,Warhurst DC

更新日期：1998-01-01 00:00:00

abstract：:Trypanosomatid protozoa lack consensus promoters for RNA polymerase (RNAP) II. However, the artificial insertion of the T7 promoter (P(T7)) and the tetracycline repressor into Trypanosoma brucei cell lines expressing T7RNAP allows P(T7)-driven gene expression to be tetracycline-inducible. These cell lines provide a mo...

journal_title：Experimental parasitology

authors： Yao C,Luo J,Hsiao CH,Donelson JE,Wilson ME

更新日期：2007-07-01 00:00:00

abstract：:Insects manifest effective immune responses that include both cellular and humoral components. Morphological and quantitative aspects of cellular and humoral cooperation during nodule formation in Chrysomya megacephala hemolymph against Saccharomyces cerevisae yeast cells were demonstrated for the first time. The anal...

journal_title：Experimental parasitology

authors： Faraldo AC,Gregório EA,Lello E

更新日期：2008-03-01 00:00:00

abstract：:Cystic echinococcosis (CE) is caused by the larval stage of Echinococcus granulosus, which in this disease the metacestode develop in visceral organs especially liver and lungs. The disease is present worldwide and affects humans as well as herbivores including cattle, sheep, camels, horses and others. Benzimidazole c...

journal_title：Experimental parasitology

authors： Ahmadnia S,Moazeni M,Mohammadi-Samani S,Oryan A

更新日期：2013-10-01 00:00:00

abstract：:A molecular study was carried out to incriminate sand fly vectors of cutaneous leishmaniasis (CL) in rural areas of Sarakhs district, Khorassane-Razavi Province, northeastern Iran, in 2011. Sand flies of Sergentomyia with three species and Phlebotomus with six species respectively comprised 73.3% and 26.7% of the spec...

journal_title：Experimental parasitology

authors： Bakhshi H,Oshaghi MA,Abai MR,Rassi Y,Akhavan AA,Sheikh Z,Mohtarami F,Saidi Z,Mirzajani H,Anjomruz M

更新日期：2013-10-01 00:00:00

abstract：:Schistosomiasis remains a global health problem. In the Mekong river basin, approximately 80,000 people are at risk of infection by Schistosoma mekongi. The parasite's eggs become entrapped in the host's organs and induce massive inflammation, contributing to the pathogenesis of schistosomiasis. In addition, egg antig...

journal_title：Experimental parasitology

authors： Thiangtrongjit T,Adisakwattana P,Limpanont Y,Dekumyoy P,Nuamtanong S,Chusongsang P,Chusongsang Y,Reamtong O

更新日期：2018-08-01 00:00:00

abstract：:The opossum Didelphis marsupialis is known to be among the most important wild reservoirs of Trypanosoma cruzi and one in which the trypanosome may go through both the usual vertebrate intracellular cycle in its tissues and an extracellular cycle in the lumen of its scent glands. The species is highly resistant to hea...

journal_title：Experimental parasitology

authors： Jansen AM,Leon L,Machado GM,da Silva MH,Souza-Leão SM,Deane MP

更新日期：1991-10-01 00:00:00

abstract：:Resistance of human pathogens like Leishmania to drugs is a growing concern where the multidrug-resistant phenotype renders chemotherapy ineffective. The acquired resistance of Leishmania to antimony has promoted intense research on the mechanisms involved but the question has not been resolved yet. In this study we h...

journal_title：Experimental parasitology

authors： Singh N,Sundar S

更新日期：2017-05-01 00:00:00

abstract：:An efficient technique was developed for separating early and late stages of embryonic development in eggs of Heterodera glycines. This technique takes advantage of density changes that occur during embryogenesis in the developing embryo and egg to partition the egg within a sucrose step gradient. Sorted samples of eg...

journal_title：Experimental parasitology

authors： Burgwyn B,Cook RL,Bolla RI

更新日期：2003-11-01 00:00:00

abstract：:In rats, CL-Brener clone caused high mortality, severe acute myocarditis, and myositis that subsided completely in surviving animals. Accordingly, no parasite kDNA could be amplified in several organs after 4 months. The monoclonal JG strain caused null mortality, acute predominantly focal myocarditis, discrete and fo...

journal_title：Experimental parasitology

authors： Franco DJ,Vago AR,Chiari E,Meira FC,Galvão LM,Machado CR

更新日期：2003-05-01 00:00:00

abstract：:African trypanosomes exhibit dramatic developmental changes in carbohydrate and energy metabolism, with cytochrome-mediated respiration playing an important role only in the insect stages. The parasites possess three isozymes of malate dehydrogenase, each of which has a different subcellular localization and, likely, ...

journal_title：Experimental parasitology

authors： Anderson SA,Carter V,Parsons M

更新日期：1998-05-01 00:00:00

abstract：:Neospora caninum causes abortion in cattle and neurological disorders in dogs. The immunological response to this parasite has been described as predominantly of the Th1 type. However, infected primary glial cell cultures release IL-10 and IL-6 but not IFN-γ. This suggests a rather protective response of the glia to a...

journal_title：Experimental parasitology

authors： Jesus EE,Pinheiro AM,Santos AB,Freire SM,Tardy MB,El-Bachá RS,Costa SL,Costa MF

更新日期：2013-03-01 00:00:00

abstract：:Leishmania parasites lack catalase and therefore, their anti-oxidant system hinges primarily upon non-protein thiols; accordingly, depletion of thiols could potentially serve as an effective drug target. We have developed a flow cytometry based assay using 5-chloromethyl fluorescein diacetate based upon its selective ...

journal_title：Experimental parasitology

authors： Sarkar A,Mandal G,Singh N,Sundar S,Chatterjee M

更新日期：2009-08-01 00:00:00

abstract：:One- and two-dimensional gel electrophoresis of the solubilized mitochondrial proteins of bloodstream and procyclic trypomastigote Trypanosoma brucei rhodesiense and radiolabeling of proteins in the presence of cycloheximide were used to identify proteins synthesized in the trypanosome mitochondrion. The proteins whic...

journal_title：Experimental parasitology

authors： Le Febvre RB,Hill GC

更新日期：1986-08-01 00:00:00

abstract：:Matrix metalloproteinases (MMPs) constitute a large family of Zn(2+) and Ca(2+) dependent endopeptidases implicated in tissue remodeling and chronic inflammation. MMPs also play key roles in the activation of growth factors, chemokines and cytokines produced by many cell types, including lymphocytes, granulocytes, and...

journal_title：Experimental parasitology

authors： de Pinho RT,da Silva WS,de Castro Côrtes LM,da Silva Vasconcelos Sousa P,de Araujo Soares RO,Alves CR

更新日期：2014-12-01 00:00:00

abstract：:The anthelmintic fenbendazole (FBZ) induces nuclear DNA fragmentation (DF) in intestinal cells of Haemonchus contortus. The DNA fragments had 3'-OH, which suggests involvement of a neutral DNase. To identify candidate DNase(s) involved, DNase activity in H. contortus intestine and other worm fractions was characterize...

journal_title：Experimental parasitology

authors： Kwak D,Jasmer DP

更新日期：2003-07-01 00:00:00

abstract：:During its life cycle the malarial parasite Plasmodium forms three invasive stages which have to invade different and specific cells for replication to ensue. Invasion is vital to parasite survival and consequently proteins responsible for invasion are considered to be candidate vaccine/drug targets. Plasmodium perfor...

journal_title：Experimental parasitology

authors： Ecker A,Pinto SB,Baker KW,Kafatos FC,Sinden RE

更新日期：2007-08-01 00:00:00

abstract：:The blood dwelling stages of schistosomes have acetylcholinesterase (AChE) and nicotinic-like acetylcholine receptors (nAChR) on their teguments. Both AChE and nAChR are concentrated on the dorsal surface of the adult male, a major surface for nutrient uptake for the worm pair. Exposure of tegumental AChE and nAChR to...

journal_title：Experimental parasitology

authors： Camacho M,Agnew A

更新日期：1995-12-01 00:00:00