Abstract:
:Plasmodium falciparum is usually asynchronous during in vitro culture. Highly synchronized cultures of P. falciparum are routinely used in malaria research. Here, we describe a simple synchronization procedure for P. falciparum asexual erythrocytic culture, which involves storage at 4°C for 8-24 h followed by routine culture. When cultures with 27-60% of ring stage were synchronized using this procedure, 70-93% ring stages were obtained after 48 h of culture and relative growth synchrony remained for at least two erythrocytic cycles. To test the suitability of this procedure for subsequent work, drug sensitivity assays were performed using four laboratory strains and four freshly adapted clinical P. falciparum isolates. Parasites synchronized by sorbitol treatment or refrigeration showed similar dose-response curves and comparable IC50 values to four antimalarial drugs. The refrigeration synchronization method is simple, inexpensive, time-saving, and should be especially useful when large numbers of P. falciparum culture are handled.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Yuan L,Hao M,Wu L,Zhao Z,Rosenthal BM,Li X,He Y,Sun L,Feng G,Xiang Z,Cui L,Yang Zdoi
10.1016/j.exppara.2014.03.010subject
Has Abstractpub_date
2014-05-01 00:00:00pages
18-23eissn
0014-4894issn
1090-2449pii
S0014-4894(14)00044-7journal_volume
140pub_type
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