Refrigeration provides a simple means to synchronize in vitro cultures of Plasmodium falciparum.


:Plasmodium falciparum is usually asynchronous during in vitro culture. Highly synchronized cultures of P. falciparum are routinely used in malaria research. Here, we describe a simple synchronization procedure for P. falciparum asexual erythrocytic culture, which involves storage at 4°C for 8-24 h followed by routine culture. When cultures with 27-60% of ring stage were synchronized using this procedure, 70-93% ring stages were obtained after 48 h of culture and relative growth synchrony remained for at least two erythrocytic cycles. To test the suitability of this procedure for subsequent work, drug sensitivity assays were performed using four laboratory strains and four freshly adapted clinical P. falciparum isolates. Parasites synchronized by sorbitol treatment or refrigeration showed similar dose-response curves and comparable IC50 values to four antimalarial drugs. The refrigeration synchronization method is simple, inexpensive, time-saving, and should be especially useful when large numbers of P. falciparum culture are handled.


Exp Parasitol


Yuan L,Hao M,Wu L,Zhao Z,Rosenthal BM,Li X,He Y,Sun L,Feng G,Xiang Z,Cui L,Yang Z




Has Abstract


2014-05-01 00:00:00












  • Trypanosoma cruzi: possible control of parasite transmission by blood transfusion using amphiphilic cationic drugs.

    abstract::About 200 clinically used amphiphilic cationic drugs have been shown to be active in vitro against Trypanosoma cruzi at concentrations of less than or equal to 1 mM. Activity against epimastigote and trypomastigote forms was similar, and in both cases the most potent drugs were litracene, maprotiline, thioproperazine,...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Hammond DJ,Hogg J,Gutteridge WE

    更新日期:1985-08-01 00:00:00

  • The effect of viroid infection of citrus trees on the amoebicidal activity of 'Maltese half-blood' (Citrus sinensis) against trophozoite stage of Acanthamoeba castellanii Neff.

    abstract::In order to promote a local Tunisian product, this study was designed to examine, for the first time, the anti-Acanthamoeba activity (Acanthamoeba castellanii Neff) of the essential oils of Tunisian Citrus sinensis peels (Maltese half-blood) and the effect of viroid plant infection on this activity. To do so, three sa...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Zouaghi G,Najar A,Chiboub O,Sifaoui I,Abderrabba M,Lorenzo Morales J

    更新日期:2017-12-01 00:00:00

  • Plasmodium falciparum and P. knowlesi: initial identification and characterization of malaria synthesized glycolipids.

    abstract::This is the first report establishing the existence of glycolipids synthesized by plasmodia, in particular Plasmodium falciparum. Trophozoites, schizonts, gametocytes, and gametes were metabolically labeled in vitro with [3H]glucosamine, [3H]galactose, [3H]glucose, [3H]mannose, [3H]fucose, [32P]inorganic phosphate, or...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Sherwood JA,Spitalnik SL,Aley SB,Quakyi IA,Howard RJ

    更新日期:1986-08-01 00:00:00

  • Babesia bigemina: in vitro multiplication of sporokinetes in Ixodes scapularis (IDE8) cells.

    abstract::This paper describes the in vitro multiplication process of Babesia bigemina sporokinetes in a cell line (IDE8) from Ixodes scapularis ticks. The inoculum was obtained from hemolymph of engorged females of Rhipicephalus (Boophilus) microplus ticks naturally infected with B. bigemina. These ticks had been fed on calves...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Ribeiro MF,Bastos CV,Vasconcelos MM,Passos LM

    更新日期:2009-07-01 00:00:00

  • Molecular cloning and characterization of major vault protein of Echinococcus multilocularis.

    abstract::The cDNA clone coding a major vault protein (MVP)-like protein was derived from Echinococcus multilocularis cysts. MVP is a main component of vault particles, which are the largest cytoplasmic ribonucleoprotein particles in eukaryotic cells. We sequenced and characterized E. multilocularis MVP (EmMVP). The nucleotide ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Goto A,Kouguchi H,Yamano K,Sawada Y

    更新日期:2013-05-01 00:00:00

  • Plasmodium falciparum: naturally occurring rabbit immunoglobulins recognize human band 3 peptide motifs and malaria-infected red cells.

    abstract::The reactivity to overlapping decapeptides based on sequences of human band 3 protein was determined for 24 rabbit serum samples. A high percentage of the sera recognized sequences of amino acid 650-670, as well as several other sequences representing putative exofacial regions of the band 3 protein. When sera were us...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Crandall I,Guthrie N,Sherman IW

    更新日期:1996-01-01 00:00:00

  • Characterization of the protein tyrosine phosphatase PRL from Entamoeba histolytica.

    abstract::Protein tyrosine phosphatase of regenerating liver (PRL) is a group of phosphatases that has not been broadly studied in protozoan parasites. In humans, PRLs are involved in metastatic cancer, the promotion of cell migration and invasion. PTPs have been increasingly recognized as important effectors of host-pathogen i...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Ramírez-Tapia AL,Baylón-Pacheco L,Espíritu-Gordillo P,Rosales-Encina JL

    更新日期:2015-12-01 00:00:00

  • Trypanosoma cruzi: the Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor.

    abstract::Tc-85, an 85-kDa surface glycoprotein specific for the trypomastigote stage of Trypanosoma cruzi, has been implicated in the invasion of host cells by the parasite. Radioactive palmitic acid was incorporated into Tc-85 immunoprecipitated from the culture medium with the H1A10 monoclonal antibody, suggesting that shedd...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Abuin G,Couto AS,de Lederkremer RM,Casal OL,Galli C,Colli W,Alves MJ

    更新日期:1996-04-01 00:00:00

  • Trypanosoma musculi: characterization of the T-lymphocyte dependency of immunity by selective immunomodulation of the mouse, Mus musculus.

    abstract::Trypanosoma musculi is a hemoflagellate parasite producing a characteristic course of infection in the mouse, terminating in elimination of the parasite from the vascular system with permanent immunity to reinfection. While this elimination is known to require functional T lymphocytes, the role of these cells has rema...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: House RV,Dean JH

    更新日期:1988-10-01 00:00:00

  • Insights into the biological features of the antigenic determinants recognized by four monoclonal antibodies in redia and adult stages of the liver fluke Fasciola hepatica.

    abstract::Fasciola hepatica is a digenean trematode which infects a wide variety of domestic animals and also humans. Previous studies have demonstrated that four monoclonal antibodies (Mabs) against the total extract of F. hepatica redia (named as 1E4, 6G11, 4E5 and 4G11) also recognized the excretion - secretion antigens (ES ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Alba A,Sánchez J,Hernández H,Mosqueda M,Rodríguez SY,Capó V,Otero O,Alfonso C,Marcet R,Sarracent J

    更新日期:2016-09-01 00:00:00

  • Acanthamoeba spp.: in vitro effects of clinical isolates on murine macrophages, osteosarcoma and HeLa cells.

    abstract::Three different cell lines (murine macrophages, HeLa and osteosarcoma cells) were assayed in order to check for the manifestation of the cytopathic effects of three strains of Acanthamoeba recently isolated in our laboratory from contact lens cases: CLC-16, CLC-41.r and CLC-51-l. Adhesion and cytotoxicity assays were ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Martín-Navarro CM,Lorenzo-Morales J,Machín RP,López-Arencibia A,Valladares B,Piñero JE

    更新日期:2010-09-01 00:00:00

  • Optimized protocols for improving the likelihood of cloning recombinant progeny from Plasmodium yoelii genetic crosses.

    abstract::Genetic cross is a powerful tool for studying malaria genes contributing to drug resistance, parasite development, and pathogenesis. Cloning and identification of recombinant progeny (RP) is laborious and expensive, especially when a large proportion of progeny derived from self-fertilization are present in the unclon...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Qi Y,Zhu F,Li J,Fu Y,Pattaradilokrat S,Hong L,Liu S,Huang F,Xu W,Su XZ

    更新日期:2013-01-01 00:00:00

  • Comparative immunoprophylactic efficacy of Haemonchus contortus recombinant enolase (rHcENO) and Con A purified native glycoproteins in sheep.

    abstract::Haemonchus contortus is the most economically important blood feeding nematode parasite of sheep and goats all over the world. Enolase in helminth parasites is a multi-functional enzyme which involves in glycolysis and host tissue invasion. In this study, the recombinant H. contortus enolase (rHcENO) was evaluated for...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Kalyanasundaram A,Jawahar S,Ilangopathy M,Palavesam A,Raman M

    更新日期:2015-07-01 00:00:00

  • Schistosoma mansoni: cercaricidal effect of 2-tetradecenoic acid, a penetration stimulant.

    abstract::Schistosoma mansoni cercariae are stimulated by 2-tetradecenoic acid (TDA) to penetrate agar substrates. TDA simultaneously causes tegumental transformation similar to that seen when cercariae transform to schistosomula, reduces the Cercarienhüllen reaction in immune human serum, and reduces larval tolerance to water....

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Haas W

    更新日期:1984-10-01 00:00:00

  • Schistosoma mansoni: protein phosphorylation during transformation of cercariae to schistosomula.

    abstract::Infectivity of the multicellular pathogen Schistosoma mansoni for the human host is dependent upon the ability of free-living cercariae to transform rapidly into parasitic schistosomula. The biochemical pathways that regulate this transitional period are unknown. The role of protein phosphorylation was investigated by...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Wiest PM,Olds GR,Bowen WD

    更新日期:1991-08-01 00:00:00

  • Haemonchus contortus: evidence that the 3A3 collagen gene is a member of an evolutionarily conserved family of nematode cuticle collagens.

    abstract::Rabbit antisera were raised against an 18 amino acid-long peptide that corresponds to the predicted sequence of the carboxy-terminal, nontriple helical region of the Haemonchus contortus 3A3 collagen gene. This sequence is highly conserved and diagnostic for members of the col-l collagen family, which includes the 3A3...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Cox GN,Shamansky LM,Boisvenue RJ

    更新日期:1990-02-01 00:00:00

  • Identification of α-11 giardin as a flagellar and surface component of Giardia lamblia.

    abstract::Giardia lamblia is a protozoan pathogen with distinct cytoskeletal structures, including median bodies and eight flagella. In this study, we examined components comprising G. lamblia flagella. Crude flagellar extracts were prepared from G. lamblia trophozoites, and analyzed by two-dimensional (2-D) gel electrophoresis...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Kim J,Lee HY,Lee MA,Yong TS,Lee KH,Park SJ

    更新日期:2013-10-01 00:00:00

  • Haemonchus contortus: ivermectin-induced paralysis of the pharynx.

    abstract::How the avermectins cause the elimination of gastrointestinal nematodes from host animals has not yet been clearly identified. Using visual and radiometric parameters to measure oral ingestion in Haemonchus contortus, we showed that ivermectin (IVM) rapidly inhibited ingestion at concentrations > or = 10(-10) M. Motil...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Geary TG,Sims SM,Thomas EM,Vanover L,Davis JP,Winterrowd CA,Klein RD,Ho NF,Thompson DP

    更新日期:1993-08-01 00:00:00

  • Schistosoma mansoni: lack of correlation between praziquantel-induced intra-worm calcium influx and parasite death.

    abstract::The schistosomicidal activity of praziquantel (PZQ) is accompanied by a large influx of calcium into the worms, suggesting that this phenomenon could be the source of the observed muscular contraction, surface disruption and eventual death of the parasite. We have incubated live adult schistosomes in a medium containi...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Pica-Mattoccia L,Orsini T,Basso A,Festucci A,Liberti P,Guidi A,Marcatto-Maggi AL,Nobre-Santana S,Troiani AR,Cioli D,Valle C

    更新日期:2008-07-01 00:00:00

  • Ascaris suum: role of ecdysteroids in molting.

    abstract::Three studies were conducted to examine the function of ecdysteroids in the development of parasitic nematodes. Ecdysone and 20-hydroxyecdysone were extracted, separated chromatographically, and measured in the reproductive tracts of adult female Ascaris suum. Perienteric fluid and the body wall did not contain measur...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Fleming MW

    更新日期:1985-10-01 00:00:00

  • Characterization of an antigenic serine protease in the Trichinella spiralis adult.

    abstract::Serine proteases have been identified as important molecules that are involved in many parasitic infections, and these molecules have also been suggested to play important roles in Trichinella spiralis infections. In the present study, the antigenic serine protease gene Ts-ADSp-7, which was screened from a cDNA librar...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Gao H,Tang B,Bai X,Wang L,Wu X,Shi H,Wang X,Liu X,Liu M

    更新日期:2018-12-01 00:00:00

  • Entamoeba histolytica: identification of EhGPCR-1, a novel putative G protein-coupled receptor that binds to EhRabB.

    abstract::EhRabB is an Entamoeba histolytica protein involved in phagocytosis. However, proteins that regulate the EhRabB activity are unknown. Here, we report the identification of a putative G protein-coupled receptor of E. histolytica (EhGPCR-1) that binds to EhRabB. By two-hybrid screening, we found a 372-bp cDNA fragment t...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Picazarri K,Luna-Arias JP,Carrillo E,Orozco E,Rodriguez MA

    更新日期:2005-07-01 00:00:00

  • Glucose-6-phosphate dehydrogenase from Plasmodium berghei: kinetic and electrophoretic characterization.

    abstract::Evidence is given for the existence of a parasite-specific glucose-6-phosphate dehydrogenase (G6PD) in Plasmodium berghei by characterization of its kinetic and electrophoretic properties. From infected rat erythrocytes the parasites were isolated, washed, and lysed. G6PD was purified by affinity chromatography with 2...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Buckwitz D,Jacobasch G,Kuckelkorn U,Plonka A,Gerth C

    更新日期:1990-04-01 00:00:00

  • Characterization and cellular distribution of heat-shock proteins HSP70 and HSP60 in Trypanosoma cruzi.

    abstract::At least eight protein members of HSP70 and three of the HSP60 families have been identified in Trypanosoma cruzi; of these, five HSP70 isoforms and one HSP60 isoform were respectively induced by a 2-hr heat-shock treatment at 37 degrees C. Immunoelectronmicroscopy of epimastigote, spheromastigote, and metacyclic cell...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Giambiagi-deMarval M,Souto-Padrón T,Rondinelli E

    更新日期:1996-08-01 00:00:00

  • Towards tissue specific transcriptomics and expression pattern analysis in schistosomes using laser microdissection microscopy.

    abstract::One difficulty facing post-genomic analyses of schistosomes is the limited data on sites of expression of many gene products expressed by the parasites in their hosts. The potential for use of laser microdissection microscopy as a preparative technique for transcriptional and proteomic profiling is reviewed. This tech...

    journal_title:Experimental parasitology

    pub_type: 杂志文章,评审


    authors: Jones MK,Higgins T,Stenzel DJ,Gobert GN

    更新日期:2007-11-01 00:00:00

  • Trypanosoma rhodesiense: mitochondrial proteins of bloodstream and procyclic trypomastigotes.

    abstract::One- and two-dimensional gel electrophoresis of the solubilized mitochondrial proteins of bloodstream and procyclic trypomastigote Trypanosoma brucei rhodesiense and radiolabeling of proteins in the presence of cycloheximide were used to identify proteins synthesized in the trypanosome mitochondrion. The proteins whic...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Le Febvre RB,Hill GC

    更新日期:1986-08-01 00:00:00

  • Schistosoma mansoni: surface membrane stability in vitro and in vivo.

    abstract::The human complement component C3b is known to bind in vitro to the surfaces of all developmental stages of schistosomes as a consequence of complement activation by the alternative pathway. C3b bound to Schistosoma mansoni parasites has now been used in combination with fluorescent labeled antibodies against C3b to l...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Ruppel A,McLaren DJ

    更新日期:1986-10-01 00:00:00

  • Plasmodium falciparum: variations in the C-terminal cysteine-rich region of the merozoite surface protein-1 in field samples among Indian isolates.

    abstract::The cysteine-rich C-terminal region of the merozoite surface protein-1, MSP-119, of Plasmodium falciparum has been the most promising vaccine target antigen to date, based on protective immunization studies with recombinant proteins in mice and monkey models. To be further developed as a vaccine candidate, it is essen...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Lalitha PV,Malhotra P,Chattopadhyay R,Chauhan VS

    更新日期:1999-05-01 00:00:00

  • Copper and selenium: auxiliary measure to control infection by Haemonchus contortus in lambs.

    abstract::The aim of this study was to evaluate the effects of selenium and copper on oxidative stress and its performance in lambs experimentally infected with Haemonchus contortus. Twenty-eight five-months old lambs were experimentally infected by the oral route with 5000 third-stage infective larvae and allocated into four g...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Leal ML,Pivoto FL,Fausto GC,Aires AR,Grando TH,Roos DH,Sudati JH,Wagner C,Costa MM,Molento MB,da Rocha JB

    更新日期:2014-09-01 00:00:00

  • Massive excystation of Toxoplasma gondii sporozoites.

    abstract::In the present study, several stimuli were tested to liberate Toxoplasma gondii sporozoites from oocysts. Incubation in Na(2)CO(3)-CO(2) buffer at 40 degrees C with ultrasound, followed by incubation at 40 degrees C with bile or deoxycholic acid, was the only method that resulted in 95% liberation of motile infective ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章


    authors: Freyre A,Falcón J

    更新日期:2004-05-01 00:00:00