Abstract：
:Plasmodium falciparum is usually asynchronous during in vitro culture. Highly synchronized cultures of P. falciparum are routinely used in malaria research. Here, we describe a simple synchronization procedure for P. falciparum asexual erythrocytic culture, which involves storage at 4°C for 8-24 h followed by routine culture. When cultures with 27-60% of ring stage were synchronized using this procedure, 70-93% ring stages were obtained after 48 h of culture and relative growth synchrony remained for at least two erythrocytic cycles. To test the suitability of this procedure for subsequent work, drug sensitivity assays were performed using four laboratory strains and four freshly adapted clinical P. falciparum isolates. Parasites synchronized by sorbitol treatment or refrigeration showed similar dose-response curves and comparable IC50 values to four antimalarial drugs. The refrigeration synchronization method is simple, inexpensive, time-saving, and should be especially useful when large numbers of P. falciparum culture are handled.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Yuan L,Hao M,Wu L,Zhao Z,Rosenthal BM,Li X,He Y,Sun L,Feng G,Xiang Z,Cui L,Yang Zdoi
10.1016/j.exppara.2014.03.010subject
Has Abstractpub_date
2014-05-01 00:00:00pages
18-23eissn
0014-4894issn
1090-2449pii
S0014-4894(14)00044-7journal_volume
140pub_type
杂志文章abstract：:About 200 clinically used amphiphilic cationic drugs have been shown to be active in vitro against Trypanosoma cruzi at concentrations of less than or equal to 1 mM. Activity against epimastigote and trypomastigote forms was similar, and in both cases the most potent drugs were litracene, maprotiline, thioproperazine,...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/s0014-4894(85)80020-5
更新日期：1985-08-01 00:00:00
abstract：:In order to promote a local Tunisian product, this study was designed to examine, for the first time, the anti-Acanthamoeba activity (Acanthamoeba castellanii Neff) of the essential oils of Tunisian Citrus sinensis peels (Maltese half-blood) and the effect of viroid plant infection on this activity. To do so, three sa...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2017.09.006
更新日期：2017-12-01 00:00:00
abstract：:This is the first report establishing the existence of glycolipids synthesized by plasmodia, in particular Plasmodium falciparum. Trophozoites, schizonts, gametocytes, and gametes were metabolically labeled in vitro with [3H]glucosamine, [3H]galactose, [3H]glucose, [3H]mannose, [3H]fucose, [32P]inorganic phosphate, or...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(86)90016-0
更新日期：1986-08-01 00:00:00
abstract：:This paper describes the in vitro multiplication process of Babesia bigemina sporokinetes in a cell line (IDE8) from Ixodes scapularis ticks. The inoculum was obtained from hemolymph of engorged females of Rhipicephalus (Boophilus) microplus ticks naturally infected with B. bigemina. These ticks had been fed on calves...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2009.03.011
更新日期：2009-07-01 00:00:00
abstract：:The cDNA clone coding a major vault protein (MVP)-like protein was derived from Echinococcus multilocularis cysts. MVP is a main component of vault particles, which are the largest cytoplasmic ribonucleoprotein particles in eukaryotic cells. We sequenced and characterized E. multilocularis MVP (EmMVP). The nucleotide ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2013.02.015
更新日期：2013-05-01 00:00:00
abstract：:The reactivity to overlapping decapeptides based on sequences of human band 3 protein was determined for 24 rabbit serum samples. A high percentage of the sera recognized sequences of amino acid 650-670, as well as several other sequences representing putative exofacial regions of the band 3 protein. When sera were us...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1996.0006
更新日期：1996-01-01 00:00:00
abstract：:Protein tyrosine phosphatase of regenerating liver (PRL) is a group of phosphatases that has not been broadly studied in protozoan parasites. In humans, PRLs are involved in metastatic cancer, the promotion of cell migration and invasion. PTPs have been increasingly recognized as important effectors of host-pathogen i...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2015.09.014
更新日期：2015-12-01 00:00:00
abstract：:Tc-85, an 85-kDa surface glycoprotein specific for the trypomastigote stage of Trypanosoma cruzi, has been implicated in the invasion of host cells by the parasite. Radioactive palmitic acid was incorporated into Tc-85 immunoprecipitated from the culture medium with the H1A10 monoclonal antibody, suggesting that shedd...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1996.0036
更新日期：1996-04-01 00:00:00
abstract：:Trypanosoma musculi is a hemoflagellate parasite producing a characteristic course of infection in the mouse, terminating in elimination of the parasite from the vascular system with permanent immunity to reinfection. While this elimination is known to require functional T lymphocytes, the role of these cells has rema...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(88)90013-6
更新日期：1988-10-01 00:00:00
abstract：:Fasciola hepatica is a digenean trematode which infects a wide variety of domestic animals and also humans. Previous studies have demonstrated that four monoclonal antibodies (Mabs) against the total extract of F. hepatica redia (named as 1E4, 6G11, 4E5 and 4G11) also recognized the excretion - secretion antigens (ES ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2016.06.010
更新日期：2016-09-01 00:00:00
abstract：:Three different cell lines (murine macrophages, HeLa and osteosarcoma cells) were assayed in order to check for the manifestation of the cytopathic effects of three strains of Acanthamoeba recently isolated in our laboratory from contact lens cases: CLC-16, CLC-41.r and CLC-51-l. Adhesion and cytotoxicity assays were ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2009.10.011
更新日期：2010-09-01 00:00:00
abstract：:Genetic cross is a powerful tool for studying malaria genes contributing to drug resistance, parasite development, and pathogenesis. Cloning and identification of recombinant progeny (RP) is laborious and expensive, especially when a large proportion of progeny derived from self-fertilization are present in the unclon...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2012.10.011
更新日期：2013-01-01 00:00:00
abstract：:Haemonchus contortus is the most economically important blood feeding nematode parasite of sheep and goats all over the world. Enolase in helminth parasites is a multi-functional enzyme which involves in glycolysis and host tissue invasion. In this study, the recombinant H. contortus enolase (rHcENO) was evaluated for...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2015.04.016
更新日期：2015-07-01 00:00:00
abstract：:Schistosoma mansoni cercariae are stimulated by 2-tetradecenoic acid (TDA) to penetrate agar substrates. TDA simultaneously causes tegumental transformation similar to that seen when cercariae transform to schistosomula, reduces the Cercarienhüllen reaction in immune human serum, and reduces larval tolerance to water....
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(84)90037-7
更新日期：1984-10-01 00:00:00
abstract：:Infectivity of the multicellular pathogen Schistosoma mansoni for the human host is dependent upon the ability of free-living cercariae to transform rapidly into parasitic schistosomula. The biochemical pathways that regulate this transitional period are unknown. The role of protein phosphorylation was investigated by...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(91)90024-q
更新日期：1991-08-01 00:00:00
abstract：:Rabbit antisera were raised against an 18 amino acid-long peptide that corresponds to the predicted sequence of the carboxy-terminal, nontriple helical region of the Haemonchus contortus 3A3 collagen gene. This sequence is highly conserved and diagnostic for members of the col-l collagen family, which includes the 3A3...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(90)90098-w
更新日期：1990-02-01 00:00:00
abstract：:Giardia lamblia is a protozoan pathogen with distinct cytoskeletal structures, including median bodies and eight flagella. In this study, we examined components comprising G. lamblia flagella. Crude flagellar extracts were prepared from G. lamblia trophozoites, and analyzed by two-dimensional (2-D) gel electrophoresis...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2013.07.010
更新日期：2013-10-01 00:00:00
abstract：:How the avermectins cause the elimination of gastrointestinal nematodes from host animals has not yet been clearly identified. Using visual and radiometric parameters to measure oral ingestion in Haemonchus contortus, we showed that ivermectin (IVM) rapidly inhibited ingestion at concentrations > or = 10(-10) M. Motil...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1993.1064
更新日期：1993-08-01 00:00:00
abstract：:The schistosomicidal activity of praziquantel (PZQ) is accompanied by a large influx of calcium into the worms, suggesting that this phenomenon could be the source of the observed muscular contraction, surface disruption and eventual death of the parasite. We have incubated live adult schistosomes in a medium containi...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2008.03.012
更新日期：2008-07-01 00:00:00
abstract：:Three studies were conducted to examine the function of ecdysteroids in the development of parasitic nematodes. Ecdysone and 20-hydroxyecdysone were extracted, separated chromatographically, and measured in the reproductive tracts of adult female Ascaris suum. Perienteric fluid and the body wall did not contain measur...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(85)90024-4
更新日期：1985-10-01 00:00:00
abstract：:Serine proteases have been identified as important molecules that are involved in many parasitic infections, and these molecules have also been suggested to play important roles in Trichinella spiralis infections. In the present study, the antigenic serine protease gene Ts-ADSp-7, which was screened from a cDNA librar...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2018.09.009
更新日期：2018-12-01 00:00:00
abstract：:EhRabB is an Entamoeba histolytica protein involved in phagocytosis. However, proteins that regulate the EhRabB activity are unknown. Here, we report the identification of a putative G protein-coupled receptor of E. histolytica (EhGPCR-1) that binds to EhRabB. By two-hybrid screening, we found a 372-bp cDNA fragment t...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2005.02.014
更新日期：2005-07-01 00:00:00
abstract：:Evidence is given for the existence of a parasite-specific glucose-6-phosphate dehydrogenase (G6PD) in Plasmodium berghei by characterization of its kinetic and electrophoretic properties. From infected rat erythrocytes the parasites were isolated, washed, and lysed. G6PD was purified by affinity chromatography with 2...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(90)90108-o
更新日期：1990-04-01 00:00:00
abstract：:At least eight protein members of HSP70 and three of the HSP60 families have been identified in Trypanosoma cruzi; of these, five HSP70 isoforms and one HSP60 isoform were respectively induced by a 2-hr heat-shock treatment at 37 degrees C. Immunoelectronmicroscopy of epimastigote, spheromastigote, and metacyclic cell...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1996.0081
更新日期：1996-08-01 00:00:00
abstract：:One difficulty facing post-genomic analyses of schistosomes is the limited data on sites of expression of many gene products expressed by the parasites in their hosts. The potential for use of laser microdissection microscopy as a preparative technique for transcriptional and proteomic profiling is reviewed. This tech...
journal_title：Experimental parasitology
pub_type： 杂志文章,评审
doi：10.1016/j.exppara.2007.06.004
更新日期：2007-11-01 00:00:00
abstract：:One- and two-dimensional gel electrophoresis of the solubilized mitochondrial proteins of bloodstream and procyclic trypomastigote Trypanosoma brucei rhodesiense and radiolabeling of proteins in the presence of cycloheximide were used to identify proteins synthesized in the trypanosome mitochondrion. The proteins whic...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(86)90011-1
更新日期：1986-08-01 00:00:00
abstract：:The human complement component C3b is known to bind in vitro to the surfaces of all developmental stages of schistosomes as a consequence of complement activation by the alternative pathway. C3b bound to Schistosoma mansoni parasites has now been used in combination with fluorescent labeled antibodies against C3b to l...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(86)90027-5
更新日期：1986-10-01 00:00:00
abstract：:The cysteine-rich C-terminal region of the merozoite surface protein-1, MSP-119, of Plasmodium falciparum has been the most promising vaccine target antigen to date, based on protective immunization studies with recombinant proteins in mice and monkey models. To be further developed as a vaccine candidate, it is essen...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1999.4401
更新日期：1999-05-01 00:00:00
abstract：:The aim of this study was to evaluate the effects of selenium and copper on oxidative stress and its performance in lambs experimentally infected with Haemonchus contortus. Twenty-eight five-months old lambs were experimentally infected by the oral route with 5000 third-stage infective larvae and allocated into four g...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2014.06.005
更新日期：2014-09-01 00:00:00
abstract：:In the present study, several stimuli were tested to liberate Toxoplasma gondii sporozoites from oocysts. Incubation in Na(2)CO(3)-CO(2) buffer at 40 degrees C with ultrasound, followed by incubation at 40 degrees C with bile or deoxycholic acid, was the only method that resulted in 95% liberation of motile infective ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2004.03.014
更新日期：2004-05-01 00:00:00
