Abstract:
:Opisthorchis viverrini is the only liver fluke that has been proved to be associated with cholangiocarcinoma (CCA). However, the mechanisms by which O. viverrini participates in the carcinogenesis of CCA are still unclear. To understand the biology and host-parasite interaction related to O. viverrini infection, gene expression profiling of this parasite is required. Here, we constructed the first 5' serial analysis of gene expression (5' SAGE) library of the adult O. viverrini and matched the data with the public data of O. viverrini, Clonorchis sinensis and other related Platyhelminthes and Nematodes. We obtained 12,401 unique tag sequences, of which 6515 (53%) could be matched with the 3419 transcript sequences. The two most abundant tag sequences were vitelline B precursor protein and myoglobin. Often several transcription start sites (TSS) were observed for one transcript. This finding may reflect the dynamic nature of transcriptional initiation events of O. viverrini genes in vivo.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Chutiwitoonchai N,Shen Y,Zheng H,Xiong H,Zhao G,Imtawil K,Intapan PM,Wongkham S,Wongkham Cdoi
10.1016/j.exppara.2008.08.004subject
Has Abstractpub_date
2008-12-01 00:00:00pages
306-13issue
4eissn
0014-4894issn
1090-2449pii
S0014-4894(08)00199-9journal_volume
120pub_type
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