Abstract：
:Early diagnosis and determining the infective stage are critical for effective therapy of toxoplasmosis. Owing to the progress in biotechnology, commonly used native, non-standardized diagnostic antigens should be replaced by genetically engineered antigens. The recombinant proteins are also promising components of subunit vaccines against Toxoplasma gondii infections. A strategic biological role of rhoptry proteins (ROP) in parasitophorous vacuole biogenesis and virulence of the parasite creates a necessity for an intensive study on the serological activity and immunogenicity of newly developed recombinant ROP antigens. Our findings indicate that all generated preparations of recombinant ROP5 and ROP18 antigens, expressed in Escherichia coli bacteria, are recognized by specific antibodies produced during acute and chronic infections in inbred laboratory mice. We noticed, for the first time, that ROP5 IgM antibodies are an early and sensitive marker of T. gondii infection. The proven immunoreactivity of the obtained preparations has become a premise for a further study on their utility in routine diagnosis of human and animal toxoplasmosis as well as in the immunoprevention of T. gondii infection (as the main or supplementary component of the vaccine).
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Grzybowski MM,Dziadek B,Dziadek J,Gatkowska J,Dzitko K,Długońska Hdoi
10.1016/j.exppara.2015.01.006subject
Has Abstractpub_date
2015-03-01 00:00:00pages
1-6eissn
0014-4894issn
1090-2449pii
S0014-4894(15)00007-7journal_volume
150pub_type
杂志文章abstract：:Root lesion nematodes (RLNs, Pratylenchus species) are a group of economically important migratory endoparasitic plant pathogens that attack host roots of major crops such as wheat and sugarcane, and can reduce crop yields by 7-15%. Pratylenchus thornei and Pratylenchus zeae were treated with double stranded RNA (dsRN...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2012.11.011
更新日期：2013-02-01 00:00:00
abstract：:Experimental infections of chimpanzees with Onchocerca volvulus and cattle with Onchocerca ochengi provide model systems for research in human onchocerciasis. These infections share many similarities from the standpoint of parasite biology, but little is known about the comparability of immune responses in the two sys...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.2000.4487
更新日期：2000-03-01 00:00:00
abstract：:The oxidative metabolism of peritoneal macrophages (PM) and neutrophils from nifedipine (calcium channel blocker)-treated, Plasmodium berghei (NK 65)-infected and normal infected Swiss Albino mice was studied. A significant fall in oxidative metabolism as evidenced by decreased chemiluminescence (CL) response (P less ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(92)90135-w
更新日期：1992-02-01 00:00:00
abstract：:In rats, CL-Brener clone caused high mortality, severe acute myocarditis, and myositis that subsided completely in surviving animals. Accordingly, no parasite kDNA could be amplified in several organs after 4 months. The monoclonal JG strain caused null mortality, acute predominantly focal myocarditis, discrete and fo...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/s0014-4894(03)00119-x
更新日期：2003-05-01 00:00:00
abstract：:The differential effect of fox and pig bile and its corresponding low molecular weight fraction (LMW) was investigated on the in vitro invasion of MDCK-AA7 epithelial cell monolayers by Trichinella spiralis muscle larvae. Seven invasion experiments were performed and a total of 274 cell monolayers were examined. Fox a...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2010.05.013
更新日期：2010-12-01 00:00:00
abstract：:In Trichostrongylus colubriformis-infected rabbits, epithelial cell migration rates and cell transit times along the villi were compared by radioautography on histological slides to normal values from noninfected small intestine. Regions of gut with high (upper jejunum) and low (ileum) burdens of worms were both exami...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(89)90116-1
更新日期：1989-04-01 00:00:00
abstract：:Shiraz district in south of Iran is a classical focus of Cutaneous leishmaniasis (CL) and previous research has consistently documented the etiologic agent to be Leishmania tropica and Leishmania major in urban and rural areas, respectively. However, none of the Phlebotomus sergenti, a known vector for L. tropica, of ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2010.05.020
更新日期：2010-12-01 00:00:00
abstract：:Previous studies have shown that intradermally (ID) injected Brugia pahangi L3 s migrate through various tissues and into the lymphatics of gerbils in a distinct pattern. Excretory/secretory products (ES) produced at the time of invasion of B. pahangi are likely to be important in this early migration phase of the par...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2013.08.007
更新日期：2013-10-01 00:00:00
abstract：:Ninety-one Chilean, 15 Bolivian, and 9 Argentinian Trypanosoma cruzi stocks, isolated from various hosts and vectors, were characterized by schizodeme analysis with EcoRI and MspI endonucleases. The three major similar pattern groups that emerged from this sample correlated with results of isoenzyme analysis. This res...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(92)90178-d
更新日期：1992-09-01 00:00:00
abstract：:A Taenia hydatigena model was used to assess the effect 0, 7, 14, 21, and 28 days of ensilation of minced potato on viability of tapeworm eggs. For infection of lambs, 2,000 T. hydatigena eggs were ensiled for 0, 7, 14, 21 and 28 days in minced potato at 22°C and fed to recently weaned lambs (29.9±0.76 kg). At slaught...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2013.01.003
更新日期：2013-04-01 00:00:00
abstract：:Because traditional methods used for sewage sludge parasitological examinations have low sensitivity, a new, highly effective method (own method - OM) was devised. The principle of this method is to eliminate the flocculent effect on the structure of sewage sludge by mechanically damaging floccules in the presence of ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2016.09.011
更新日期：2016-11-01 00:00:00
abstract：:Non-invasive small animal in vivo imaging is an essential tool in a broad variety of biomedical sciences and enables continuous monitoring of disease progression in order to develop and improve diagnostic, therapeutic and preventive measures. Imaging parasites non-invasively in live animals allows efficient parasite d...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2020.107905
更新日期：2020-07-01 00:00:00
abstract：:In this report, an immunodominant antigen called BgIRA from Babesia gibsoni is identified and described. A highly repetitive antigen was screened from a cDNA library. The genomic BgIRA gene exists as single cope gene and contains 10 introns. BgIRA plays a dominant role in the immune response in dogs infected with B. g...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2007.07.005
更新日期：2008-01-01 00:00:00
abstract：:The rate of phosphorylation of 2-deoxyglucose (2DG) was determined by sequential pulsing of schistosomes (Schistosoma mansoni, S. japonicum, and S. haematobium) with 3H- and 14C-labeled 2-deoxy-D-glucose. Subsequent column chromatographic separation of the neutral [3H]2DG and [14C]2DG from the 3H- and 14C-labeled 2-de...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(87)90059-2
更新日期：1987-12-01 00:00:00
abstract：:Trypanosoma congolense is an important pathogen that wreaks havoc in the livestock industry of the African continent. This study evaluated the in vivo antitrypanosomal activity of geranylacetone and its ameliorative effect on the disease-induced anaemia and organ damages as well as its inhibitory effects against trypa...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2019.04.011
更新日期：2019-06-01 00:00:00
abstract：:Canine leishmaniosis due to Leishmania infantum is a widespread zoonotic disease. Although aminosidine can be an effective treatment, current therapeutic recommendations do not advocate its use, mainly due to concerns regarding the potential nephrotoxicity and ototoxicity of this drug. The aim of this randomized, blin...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2019.107768
更新日期：2019-11-01 00:00:00
abstract：:An efficient technique was developed for separating early and late stages of embryonic development in eggs of Heterodera glycines. This technique takes advantage of density changes that occur during embryogenesis in the developing embryo and egg to partition the egg within a sucrose step gradient. Sorted samples of eg...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2003.12.007
更新日期：2003-11-01 00:00:00
abstract：:By using a sensitive fluorometric method with Val-Leu-Gly-Arg-3-amino-9-ethylcarbazole (VLGR-AEC) as a substrate, two endopeptidase activities were identified in two fractions of Sephacryl S-200 gel filtration from soluble P. berghei and P. chabaudi extracts. Controls with normal mouse erythrocytes, with leukocytes, a...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(87)90013-0
更新日期：1987-08-01 00:00:00
abstract：:Chemoattraction studies were done with Schistosoma mansoni adults from mice. To test for attraction or repulsion, some worm pairs were separated mechanically and the individuals placed in polycarbonate chambers. Experiments were done at 37 C and chambers contained dialysis tube chimneys. In all cases, heterosexual att...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(83)90070-x
更新日期：1983-10-01 00:00:00
abstract：:Chagas' disease is caused by unicellular parasite Trypanosoma cruzi (T. cruzi). It is endemic throughout Latin America, but nowadays has become a global challenge due to tourism and migration. Non-treated infection may result in health-threatening complications and lead to death. Current medications for this infection...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2016.12.013
更新日期：2017-01-01 00:00:00
abstract：:Purine uptake has been studied in many protozoan parasites in the last few years, and several of the purine transporters have been cloned. In contrast, very little is known about the salvage of preformed pyrimidines by protozoa, and no pyrimidine transporters have been cloned, yet chemotherapy based on pyrimidine nucl...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2006.02.018
更新日期：2006-10-01 00:00:00
abstract：:We probed DNA from all trypanosomatid genera by slot blot hybridization with an oligonucleotide (SL3') complementary to a sequence of the Phytomonas spliced-leader or mini-exon RNA. The 19-nucleotide probe target site was previously shown to be highly conserved among a limited number of Phytomonas isolates, but diverg...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1996.0119
更新日期：1996-12-01 00:00:00
abstract：:Entamoeba histolytica programmed cell death (PCD) induced by G418 is characterized by the release of important amounts of intracellular calcium from reservoirs. Nevertheless, no typical caspases have been detected in the parasite, the PCD phenotype is inhibited by the cysteine protease inhibitor E-64. These results st...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2015.10.005
更新日期：2015-12-01 00:00:00
abstract：:The aim of the current investigation was to assess the impacts of methanolic extract of Allium sativum (MEAS) on IL-4 (a cytokine derived from Th2 cells) and IFN-ɣ (a cytokine derived from Th1 cells) levels in mice infected with Echinococcus granulosus. Sixty healthy BALB/c female mice were used in this study. Each an...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2020.108042
更新日期：2021-01-01 00:00:00
abstract：:Schistosoma mansoni infection in humans and animals induces abnormal neurobehavioral responses following granuloma formation. In mice, granulomas in the liver are observed 8 weeks after infection, while after 15-20 weeks, the presence of eggs and granulomas in the brain has been reported. In this study, outbred CD-1 f...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1996.0047
更新日期：1996-06-01 00:00:00
abstract：:The expression and activity of topoisomerase I (PfTopoI) has been examined during the intraerythrocytic stages of the Plasmodium falciparum life cycle. The promoter is inactive during the early ring stage and becomes active only during the later trophozoite and schizont stages. The PfTOP1 transcript starts to accumula...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1998.4362
更新日期：1999-02-01 00:00:00
abstract：:An active Trypanosoma cruzi transmission cycle maintained by wild rodents in the Andean valleys of Cochabamba Bolivia is described. Wild and domestic Triatoma infestans with 60% infection with T. cruzi were found and was evidenced in 47.5% (rodents) and 26.7% (marsupial) by parasitological and/or serologycal methods. ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2006.04.010
更新日期：2006-12-01 00:00:00
abstract：:A limiting dilution assay (LDA) was developed for the quantification of Trypanosoma cruzi in the heart and blood of infected mice. Three groups of swiss mice were injected ip with "CL", "Colombiana," and "Y" strains. At 1-day intervals after infection, blood and the heart were removed. Serial blood dilutions in LIT me...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(90)90099-x
更新日期：1990-02-01 00:00:00
abstract：:The stability of the gene encoding TSA417, a 72-kDa variant-specific surface protein (VSP) produced by trophozoites of Giardia intestinalis isolate WB-C6, was investigated in isolates of similar (Assemblage A / Group I) or distinct (Assemblage A / Group II) genotype. Using primers specific for the WB-C6 tsa417 gene, D...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1998.4325
更新日期：1998-11-01 00:00:00
abstract：:The aim of this study was to analyze the antioxidant status and oxidative profile in serum and liver of rats experimentally infected with Fasciola hepatica and its relationship with pathological findings. Twenty-four rats were divided into two groups: group A consisted of 12 healthy rats and group B of 12 rats infecte...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2015.08.008
更新日期：2015-12-01 00:00:00
