Abstract：
:An efficient technique was developed for separating early and late stages of embryonic development in eggs of Heterodera glycines. This technique takes advantage of density changes that occur during embryogenesis in the developing embryo and egg to partition the egg within a sucrose step gradient. Sorted samples of eggs separated with 82% enrichment for pre-gastrula early embryos and 93% enrichment for first and second stage unhatched juveniles as late embryos. Subpopulations enriched for either developmental stage are available for use in generating stage-specific cDNA libraries, normalization of subpopulations to synchronize development, biochemical characterization, and many other uses.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Burgwyn B,Cook RL,Bolla RIdoi
10.1016/j.exppara.2003.12.007keywords:
subject
Has Abstractpub_date
2003-11-01 00:00:00pages
179-83issue
3-4eissn
0014-4894issn
1090-2449pii
S0014489403001760journal_volume
105pub_type
杂志文章abstract：:Experiments were carried out to determine whether there are stage-specific antigens on microfilariae of Brugia pahangi, using sera from Mongolian jirds infected with B. pahangi and monoclonal antibodies against microfilariae of B. pahangi. These studies showed that microfilariae have both stage-specific and nonspecifi...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(86)90130-x
更新日期：1986-02-01 00:00:00
abstract：:The schistosomicidal activity of praziquantel (PZQ) is accompanied by a large influx of calcium into the worms, suggesting that this phenomenon could be the source of the observed muscular contraction, surface disruption and eventual death of the parasite. We have incubated live adult schistosomes in a medium containi...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2008.03.012
更新日期：2008-07-01 00:00:00
abstract：:Coccidiosis is a major parasitic disease of poultry industry and an ideal vaccine should induce long-lasting cross-species protective immunity. Broiler chickens (Cobb 500) were inoculated with single, double or triple injections of a synthetic peptide (derived from sequences of Eimeria acervulina and Eimeria tenella a...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2005.03.026
更新日期：2005-08-01 00:00:00
abstract：:Five adjuvants were examined for their ability to potentiate the immune response of mice to soluble antigens from adult Nematospiroides dubius prepared by affinity chromatography against antibodies from repeatedly infected mice as ligands (IMIgAg). Immunized mice were better protected against N. dubius by IMIgAg injec...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(89)90009-x
更新日期：1989-01-01 00:00:00
abstract：:Ten pairs of Leishmania (Viannia) strains isolated from mucosal and cutaneous lesions of the same patient were analyzed genotypically in order to determine whether populations that had metastasized to mucosal sites differed from those in the cutaneous lesion. The strains were previously characterized by multi locus en...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2004.07.005
更新日期：2004-09-01 00:00:00
abstract：:Purine uptake has been studied in many protozoan parasites in the last few years, and several of the purine transporters have been cloned. In contrast, very little is known about the salvage of preformed pyrimidines by protozoa, and no pyrimidine transporters have been cloned, yet chemotherapy based on pyrimidine nucl...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2006.02.018
更新日期：2006-10-01 00:00:00
abstract：:EhRabB is an Entamoeba histolytica protein involved in phagocytosis. However, proteins that regulate the EhRabB activity are unknown. Here, we report the identification of a putative G protein-coupled receptor of E. histolytica (EhGPCR-1) that binds to EhRabB. By two-hybrid screening, we found a 372-bp cDNA fragment t...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2005.02.014
更新日期：2005-07-01 00:00:00
abstract：:Most of the drugs used for the treatment of trichinellosis show a limited bioavailability and a high degree of resistance. Therefore, this study aimed to characterize the anthelmintic potential activity of nitazoxanide (NTZ) in a rat model of experimental trichinellosis. Animals were divided into three groups; group I...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2016.08.009
更新日期：2016-11-01 00:00:00
abstract：:Living Leishmania amazonensis amastigotes were incubated with radioiodinated N-benzyloxycarbonyl-L-tyrosyl-L-alanyl diazomethane (Z-Tyr-AlaCHN2), an irreversible inhibitor of mammalian cathepsins B and L. Parasite lysates were subjected to electrophoresis in gelatin-containing sodium dodecyl sulfate-acrylamide gels to...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(91)90066-6
更新日期：1991-11-01 00:00:00
abstract：:We have identified congenic mouse strains that differ dramatically in resistance to infection with the murine malaria parasite Plasmodium yoelii 17X. After infection, BALB/c mice develop severe anemia and a high degree of parasitemia which sometimes results in death. The mtv-7 congenic strain BALB.D2.mlsa, however, de...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1997.4154
更新日期：1997-06-01 00:00:00
abstract：:Typically, antimicrobial peptides (AMPs) are short positive charged peptides serving a key role in innate immunity as well as antimicrobial activity. Discovering novel therapeutic agents is considered as an undeniable demand due to increasing microbial species with antibiotic resistance. In this direction, the unique ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2019.107823
更新日期：2020-02-01 00:00:00
abstract：:An active Trypanosoma cruzi transmission cycle maintained by wild rodents in the Andean valleys of Cochabamba Bolivia is described. Wild and domestic Triatoma infestans with 60% infection with T. cruzi were found and was evidenced in 47.5% (rodents) and 26.7% (marsupial) by parasitological and/or serologycal methods. ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2006.04.010
更新日期：2006-12-01 00:00:00
abstract：:The cDNA clone coding a major vault protein (MVP)-like protein was derived from Echinococcus multilocularis cysts. MVP is a main component of vault particles, which are the largest cytoplasmic ribonucleoprotein particles in eukaryotic cells. We sequenced and characterized E. multilocularis MVP (EmMVP). The nucleotide ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2013.02.015
更新日期：2013-05-01 00:00:00
abstract：:The development of nanotechnology has generated new means of disease diagnosis and treatment. Infectious diseases, including leishmaniasis, malaria, etc., have benefited from the advent of new nanomaterials and/or nanodevices capable of detecting specific antigens and antibodies with high specificity and low cost. In ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2011.10.015
更新日期：2012-02-01 00:00:00
abstract：:High-throughput drug screening methods against the intracellular stage of Leishmania have been facilitated by the development of in vitro models of infection. The use of cell lines rather than primary cells facilitates these methods. Peripheral blood mononuclear cell (PBMC) derived macrophages and THP-1 cells were inf...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2010.06.007
更新日期：2010-12-01 00:00:00
abstract：:Shiraz district in south of Iran is a classical focus of Cutaneous leishmaniasis (CL) and previous research has consistently documented the etiologic agent to be Leishmania tropica and Leishmania major in urban and rural areas, respectively. However, none of the Phlebotomus sergenti, a known vector for L. tropica, of ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2010.05.020
更新日期：2010-12-01 00:00:00
abstract：:The swimming behavior of Poecilia vivipara was evaluated using an image analysis system comparing laboratory-reared uninfected fish before and after experimental infection with different intensities of cercariae of the trematode Acanthocollaritrema umbilicatum. Two experiments were performed, each with 30 fish which w...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2010.10.018
更新日期：2011-02-01 00:00:00
abstract：:Chemotherapy of food-borne trematodes relies on two drugs, praziquantel and tricabendazole, and there is growing interest in finding alternative therapies. Plant oil extracts have long been used in traditional Chinese medicine as sources of bioactive compounds with antiparasitic activity. Species of the genus Echinost...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2017.01.003
更新日期：2017-03-01 00:00:00
abstract：:Due to the occurrence of genetic recombination, a reliable and discriminatory method to genotype Cryptosporidium isolates at the intra-species level requires the analysis of multiple loci, but a standardised scheme is not currently available. A workshop was held at the Robert Koch Institute, Berlin in 2016 that gather...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2018.06.004
更新日期：2018-08-01 00:00:00
abstract：:Two fasciolicide preparations have been compared in 130 rats experimentally infected with Fasciola hepatica. Parasitological, immunological, and biochemical parameters have been followed to monitor the efficacy of the treatments. While Fascinex (triclabendazole) efficiently cured both male and female rats when adminis...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.2000.4501
更新日期：2000-04-01 00:00:00
abstract：:Giardia lamblia undergo surface antigenic variation in vitro and in vivo. The presence of variant trophozoites can be detected in clones after exposure to cytotoxic monoclonal antibodies. Surviving Giardia (progeny) no longer possess the initial major surface antigen which is replaced by new antigens. Exposure of a cl...
journal_title：Experimental parasitology
pub_type： 杂志文章,评审
doi：10.1016/0014-4894(89)90104-5
更新日期：1989-02-01 00:00:00
abstract：:Schistosomiasis remains a global health problem. In the Mekong river basin, approximately 80,000 people are at risk of infection by Schistosoma mekongi. The parasite's eggs become entrapped in the host's organs and induce massive inflammation, contributing to the pathogenesis of schistosomiasis. In addition, egg antig...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2018.07.002
更新日期：2018-08-01 00:00:00
abstract：:Monoclonal antibodies have a wide range of applications in basic and applied research as well as in the medical and pharmaceutical industries. Phage display antibody libraries offer an alternative to hybridoma technology for the generation of monoclonal antibodies and can be applied to high-throughput screening and fa...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2019.107737
更新日期：2019-10-01 00:00:00
abstract：:Spleen cells from BALB/c mice were fused with myeloma cells following infection of the mice with Trichinella spiralis larvae and an ip booster injection with larval homogenate antigen. A monoclonal antibody (Mab), designated as TS 3G6 which did not react with sera or tissue extracts from noninfected mice, rats, and gu...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(91)90018-r
更新日期：1991-08-01 00:00:00
abstract：:In recent years, considerable attention has been given to identify new antileishmanial products derived from medicinal plants, although, to date, no new effective compound has been recently applied to treat leishmaniasis. In the present study, the antileishmanial activity of a water extract from Zingiber officinalis R...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2016.03.026
更新日期：2016-07-01 00:00:00
abstract：:Infectivity of the multicellular pathogen Schistosoma mansoni for the human host is dependent upon the ability of free-living cercariae to transform rapidly into parasitic schistosomula. The biochemical pathways that regulate this transitional period are unknown. The role of protein phosphorylation was investigated by...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(91)90024-q
更新日期：1991-08-01 00:00:00
abstract：:Human cysticercosis caused by Taenia crassiceps is unusual; however, it is an useful experimental model for cysticercosis studies. Benzimidazole derivatives are important antihelminthic drugs widely used against helminths. A novel compound 6-chloro-5-(1-naphthyloxy) -2-(trifluoromethyl)-1H-benzimidazole (RCB20) is a b...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2016.11.002
更新日期：2017-01-01 00:00:00
abstract：:Parasites of wildlife inhabiting urbanised and peri-urban environments are of interest regarding wildlife population health, and also veterinary public health in the case of parasites that can also infect humans and domestic animals. This study aimed to: identify, and estimate the prevalence of, species of Eimeria par...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2016.09.012
更新日期：2016-11-01 00:00:00
abstract：:Toxoplasma gondii is responsible for fetopathy in farm animals and humans and severe disease in immunocompromised individuals (i.e. AIDS patients). Effective vaccines, inducing protective and long-lasting immunity to this global parasite, are still desired. In the work, we evaluated the immunogenic and immunoprotectiv...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2007.07.003
更新日期：2008-02-01 00:00:00
abstract：:Larvae of Rhinoestrus spp. (Diptera, Oestridae) infect nasal and sinus cavities of horses, causing a nasal myiasis characterized by severe respiratory distress. Presently, the diagnosis of horse nasal botfly relies on the observation of clinical signs, on the post mortem retrieval of larvae or on molecular assays perf...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2009.11.014
更新日期：2010-04-01 00:00:00
