当前位置： SCI文献检索 > EXPERIMENTAL PARASITOLOGY期刊下所有文献 > Light and scanning electron microscopy study of in vitro effects of artesunate in newly excysted metacercariae of Echinostoma paraensei (Trematoda: Digenea).

Light and scanning electron microscopy study of in vitro effects of artesunate in newly excysted metacercariae of Echinostoma paraensei (Trematoda: Digenea).

Abstract：

:Chemotherapy of food-borne trematodes relies on two drugs, praziquantel and tricabendazole, and there is growing interest in finding alternative therapies. Plant oil extracts have long been used in traditional Chinese medicine as sources of bioactive compounds with antiparasitic activity. Species of the genus Echinostoma are used as good models to test effective compounds against food-borne trematodes. This study evaluated the anthelmintic activity of crude artesunate extracts in vitro on newly excysted metacercariae of Echinostoma paraensei by light and scanning electron microscopy (SEM). The flukes were incubated with 1 μg/mL, 10 μg/mL, 25 μg/mL, 50 μg/mL and 100 μg/mL of artesunate for 4, 12, 24, 48 and 72 h. When the exposure time and concentration of artesunate increased, there were changes in motor activity, tegument damage and death. Blebs and swelling were the most common damages quantified on the tegument. The in vitro study reproduced results described for other immature flukes incubated with artemisinin derivatives. Excysted metacercariae of E. paraensei constitute a good model to study in vitro drug effects.

journal_name

Exp Parasitol

journal_title

Experimental parasitology

authors

Souza JG,Lopes Torres EJ,Garcia JS,Gomes AP,Rodrigues-Silva R,Maldonado A Jr,Machado-Silva JR

doi

10.1016/j.exppara.2017.01.003

subject

Has Abstract

pub_date

2017-03-01 00:00:00

pages

10-16

eissn

0014-4894

issn

1090-2449

pii

S0014-4894(17)30038-3

journal_volume

174

pub_type

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