Molecular characterization of Eimeria species in macropods.

Abstract:

:A total of 597 faecal samples were collected from western grey kangaroos (Macropus fuliginosus), Euros (M. robustus), red kangaroos (M. rufus) in Western Australia and Eastern Grey Kangaroos (M. giganteus) from Victoria and screened for the presence of Eimeria by PCR at the 18S ribosomal RNA (rRNA) locus. The overall prevalence was 24.3% (145/597). At the 18S rRNA locus, sequences were obtained for 25 of the 145 positives. Phylogenetic analysis indicated that all the macropod-derived Eimeria species grouped in a separate marsupial clade that included Eimeria trichosuri from brushtail possums. At least 6 different clades were identified within the marsupial isolates and many of the genotypes identified are likely to be valid species, however morphological and biological data need to be collected to match sequences to previously characterized Eimeria species or identify if they are new species.

journal_name

Exp Parasitol

authors

Yang R,Fenwick S,Potter A,Elliot A,Power M,Beveridge I,Ryan U

doi

10.1016/j.exppara.2012.07.003

subject

Has Abstract

pub_date

2012-10-01 00:00:00

pages

216-21

issue

2

eissn

0014-4894

issn

1090-2449

pii

S0014-4894(12)00218-4

journal_volume

132

pub_type

杂志文章
  • Transtegumental diffusion of benzimidazole anthelmintics into Moniezia benedeni: correlation with their octanol-water partition coefficients.

    abstract::The experiments described here report on the correlation between the ex vivo diffusion of different benzimidazole (BZD) anthelmintics into the cestode parasite Moniezia benedeni, and their octanol-water partition coefficients (P.C.). The characterisation of the drug diffusion process into target parasites is relevant ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/s0014-4894(03)00060-2

    authors: Mottier ML,Alvarez LI,Pis MA,Lanusse CE

    更新日期:2003-01-01 00:00:00

  • Eimeria tenella: characterization of a 5S ribosomal RNA repeat unit and its use as a species-specific probe.

    abstract::The oocysts of Eimeria tenella, one of the most pathogenic of several species causing chicken coccidiosis, are difficult to distinguish microscopically from several other infective Eimeria species. One copy of a gene coding for 5S ribosomal RNA has been cloned from E. tenella and sequenced. A coding region of 120 nucl...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1993.1008

    authors: Stucki U,Braun R,Roditi I

    更新日期:1993-02-01 00:00:00

  • Rapid concentration and sensitive detection of hookworm ova from wastewater matrices using a real-time PCR method.

    abstract::The risk of human hookworm infections from land application of wastewater matrices could be high in regions with high hookworm prevalence. A rapid, sensitive and specific hookworm detection method from wastewater matrices is required in order to assess human health risks. Currently available methods used to identify h...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2015.08.009

    authors: Gyawali P,Sidhu JP,Ahmed W,Jagals P,Toze S

    更新日期:2015-12-01 00:00:00

  • Rhipicephalus (Boophilus) microplus: clotting time in tick-infested skin varies according to local inflammation and gene expression patterns in tick salivary glands.

    abstract::Ticks deposit saliva at the site of their attachment to a host in order to inhibit haemostasis, inflammation and innate and adaptive immune responses. The anti-haemostatic properties of tick saliva have been described by many studies, but few show that tick infestations or its anti-haemostatic components exert systemi...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2009.12.013

    authors: Carvalho WA,Maruyama SR,Franzin AM,Abatepaulo AR,Anderson JM,Ferreira BR,Ribeiro JM,Moré DD,Augusto Mendes Maia A,Valenzuela JG,Garcia GR,de Miranda Santos IK

    更新日期:2010-04-01 00:00:00

  • Schistosoma mansoni: possible involvement of protein kinase C in linoleic acid-induced proteolytic enzyme release from cercariae.

    abstract::The possible involvement of protein kinase C and Ca2+ metabolism in the proteolytic enzyme release from schistosome cercariae was studied. Cercariae were placed in dechlorinated tap water containing 0.37 mM calcium in the small glass petri dish and exposed to the stimuli (linoleic acid, phorbol esters, and Ca2+ ionoph...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(91)90151-l

    authors: Matsumura K,Mitsui Y,Sato K,Sakamoto M,Aoki Y

    更新日期:1991-04-01 00:00:00

  • Trichinella spiralis muscle larvae excretory/secretory products trigger apoptosis and S-phase arrest of the non-small-cell lung cancer line A549.

    abstract::Trichinella spiralis (T. spiralis) muscle larvae (ML) excretory/secretory products (ESPs) are antitumor substances extracted from the culture medium of T. spiralis ML. The ESPs inhibit tumor growth and induce tumor cell apoptosis. To explore the effects of these products on the non-small-cell lung cancer (NSCLC) line ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2020.107983

    authors: Wu H,Li M,Shao X,An Z,Du J,Yin H,Pan J,Li S,Zhang Y,Du L

    更新日期:2020-11-01 00:00:00

  • Morphological, genotypic, and physiological characterization of Acanthamoeba isolates from keratitis patients and the domestic environment in Vitoria, Espírito Santo, Brazil.

    abstract::Amoebae of the genus Acanthamoeba are free-living protozoa that can cause granulomatous encephalitis and keratitis in humans. In this study, four clinical and three household dust isolates obtained in Vitória, Espírito Santo, Brazil were characterized by their morphological, genotypic, and physiological properties. Al...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2013.05.013

    authors: Duarte JL,Furst C,Klisiowicz DR,Klassen G,Costa AO

    更新日期:2013-09-01 00:00:00

  • Brugian infections in the peritoneal cavities of laboratory mice: kinetics of infection and cellular responses.

    abstract::Standard, immunocompetent, inbred strains of mice are non-permissive for infection with the human filarial nematode, Brugia malayi or the closely related Brugia pahangi. This non-permissiveness allows one to address the mechanism(s) that might be used by mammalian hosts to eliminate large, multicellular, metazoan, ext...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/s0014-4894(02)00015-2

    authors: Rajan TV,Ganley L,Paciorkowski N,Spencer L,Klei TR,Shultz LD

    更新日期:2002-04-01 00:00:00

  • Hymenolepis diminuta: analysis of the expression of Toll-like receptor genes (TLR2 and TLR4) in the small and large intestines of rats.

    abstract::Toll receptors play a critical role in the rapid activation of innate immune responses to a variety of pathogens. In mammals, Toll-like receptors (TLR) have been found in both immune related cells and other cells. At present little is known about the participation of TLR in host defense mechanisms during parasitic inf...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2011.12.002

    authors: Kosik-Bogacka DI,Wojtkowiak-Giera A,Kolasa A,Salamatin R,Jagodzinski PP,Wandurska-Nowak E

    更新日期:2012-03-01 00:00:00

  • Echinococcus granulosus: study of the in vitro complement activation by protoscoleces by measuring the electric potential difference across the tegumental membrane.

    abstract::Complement activation by protoscoleces of Echinococcus granulosus was studied by analyzing the damage to their tegumental membrane produced by incubation in both normal and hydatid human sera. The state of the apical tegumental membrane was evaluated by measuring the electric potential difference with microelectrodes....

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(92)90211-r

    authors: Ferreira AM,Trecu T,Reisin I

    更新日期:1992-11-01 00:00:00

  • Trypanosoma cruzi: effects of repetitive stress during the development of experimental infection.

    abstract::Activation of the hypothalamus-pituitary-adrenal axis plays a major role in the suppression of the immune system. We have investigated the effects of repetitive stress on Wistar rats infected with the Y strain of Trypanosoma cruzi and a control group that underwent stressor stimuli by exposure to ether vapor for one m...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2005.03.009

    authors: Santos CD,Caldeira JC,Toldo MP,Prado JC

    更新日期:2005-06-01 00:00:00

  • Exploration of fluorescence-based real-time loop-mediated isothermal amplification (LAMP) assay for detection of Isospora suis oocysts.

    abstract::Isospora suis is an intestinal protozoan parasite in pigs. The 2-3 weeks old piglets are most often infected by I. suis because their immune system is not fully developed. The infection exhibits clinical features such as diarrhea and dehydration and seriously affects the economic interests of farmers. The traditional ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2016.03.001

    authors: Huang C,Wen F,Yue L,Chen R,Zhou W,Hu L,Chen M,Wang S

    更新日期:2016-06-01 00:00:00

  • Toxoplasma gondii: P30 peptides recognition pattern in human toxoplasmosis.

    abstract::In this study, human sera reactivity against nine peptides derived from the Toxoplasma gondii P30 protein was assessed by ELISA in patients with different clinical forms of toxoplasmosis. Same as has been reported in mice, sera from congenital, ocular and chronic asymptomatic toxoplasmosis patients recognized more str...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2009.06.017

    authors: Cardona N,de-la-Torre A,Siachoque H,Patarroyo MA,Gomez-Marin JE

    更新日期:2009-10-01 00:00:00

  • Plasmodium falciparum: synchronization of asexual development with aphidicolin, a DNA synthesis inhibitor.

    abstract::The asexual development cycle of Plasmodium falciparum, a malarial parasite of humans, has been synchronized in culture by treating ring-stage parasites with aphidicolin, an inhibitor of DNA synthesis. Optimization of both the concentration of drug added to ring stage containing red blood cells and the duration of exp...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(84)90061-4

    authors: Inselburg J,Banyal HS

    更新日期:1984-02-01 00:00:00

  • Characterization and expression of proteases during Trypanosoma cruzi metacyclogenesis.

    abstract::Investigation of protease activities during the transformation of Trypanosoma cruzi epimastigotes into metacyclic trypomastigoes (metacyclo-genesis) revealed three major components with apparent molecular weights of 65, 52, and 40 kDa. The 65-kDa protease is a metacyclic trypomastigote stage-specific protease with an ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(91)90006-i

    authors: Bonaldo MC,d'Escoffier LN,Salles JM,Goldenberg S

    更新日期:1991-07-01 00:00:00

  • Trypanosoma cruzi: Induction of benznidazole resistance in vivo and its modulation by in vitro culturing and mice infection.

    abstract::Through a continuous in vivo drug pressure protocol, using mice as experimental model, we induced benznidazole resistance in Trypanosoma cruzi stocks. Full resistance was obtained for four out of five T. cruzi stocks analyzed. However, the number of benznidazole doses (40-180), as well as the time (4-18 months) necess...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2008.09.007

    authors: Dos Santos FM,Caldas S,de Assis Cáu SB,Crepalde GP,de Lana M,Machado-Coelho GL,Veloso VM,Bahia MT

    更新日期:2008-12-01 00:00:00

  • Acanthamoeba culbertsoni: analysis of amoebic adhesion and invasion on extracellular matrix components collagen I and laminin-1.

    abstract::Acanthamoeba are free-living amoebae found in most environments that can cause brain and corneal infections. To infect humans, these pathogens must interact with host cells and the extracellular matrix (ECM). In order to define the mode by which amoebae recognize ECM components and process this recognition, we analyze...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2009.08.004

    authors: Rocha-Azevedo Bd,Jamerson M,Cabral GA,Marciano-Cabral F

    更新日期:2010-09-01 00:00:00

  • A colorimetric method for the evaluation of anti-giardial drugs in vitro.

    abstract::The aim of this study was to develop a simple and reliable method to determine the viability of Giardia intestinalis after incubation with an anti-giardial agent by using a colorimetric method. Factors that may affect the optical density value were systematically evaluated. The most suitable conditions were obtained w...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2010.09.006

    authors: Hounkong K,Sawangjaroen N,Phongpaichit S

    更新日期:2011-02-01 00:00:00

  • Vaccination of sheep with purified cysteine proteinases of Fasciola hepatica decreases worm fecundity.

    abstract::There has been some evidence from several parasite systems that proteinases might have potential as protective antigens against parasitic infection. A cysteine proteinase complex identified in the regurgitant of adult F. hepatica was examined in this context. The thiol-cathepsin-related proteinases of M(r) 28,000 were...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1994.1014

    authors: Wijffels GL,Salvatore L,Dosen M,Waddington J,Wilson L,Thompson C,Campbell N,Sexton J,Wicker J,Bowen F

    更新日期:1994-03-01 00:00:00

  • Plasmodium berghei: partial purification and characterization of the mitochondrial cytochrome c oxidase.

    abstract::Mitochondria from a rodent malarial parasite (Plasmodium berghei) were successfully purified by differential centrifugation and 22% Percoll density gradient separation. The purified mitochondria from the erythrocytic stages of the parasite had a density of 1.05 and were found to be heterogeneous by transmission electr...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1993.1070

    authors: Krungkrai J,Krungkrai SR,Bhumiratana A

    更新日期:1993-09-01 00:00:00

  • Effects of IFN-γ, TNF-α, IL-10 and TGF-β on Neospora caninum infection in rat glial cells.

    abstract::Neospora caninum causes abortion in cattle and neurological disorders in dogs. The immunological response to this parasite has been described as predominantly of the Th1 type. However, infected primary glial cell cultures release IL-10 and IL-6 but not IFN-γ. This suggests a rather protective response of the glia to a...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2012.11.016

    authors: Jesus EE,Pinheiro AM,Santos AB,Freire SM,Tardy MB,El-Bachá RS,Costa SL,Costa MF

    更新日期:2013-03-01 00:00:00

  • Trichostrongylus colubriformis: epithelial cell migration in the proximal and distal small intestine of infected rabbits.

    abstract::In Trichostrongylus colubriformis-infected rabbits, epithelial cell migration rates and cell transit times along the villi were compared by radioautography on histological slides to normal values from noninfected small intestine. Regions of gut with high (upper jejunum) and low (ileum) burdens of worms were both exami...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(89)90116-1

    authors: Hoste H,Mariana JC

    更新日期:1989-04-01 00:00:00

  • Light and scanning electron microscopy study of in vitro effects of artesunate in newly excysted metacercariae of Echinostoma paraensei (Trematoda: Digenea).

    abstract::Chemotherapy of food-borne trematodes relies on two drugs, praziquantel and tricabendazole, and there is growing interest in finding alternative therapies. Plant oil extracts have long been used in traditional Chinese medicine as sources of bioactive compounds with antiparasitic activity. Species of the genus Echinost...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2017.01.003

    authors: Souza JG,Lopes Torres EJ,Garcia JS,Gomes AP,Rodrigues-Silva R,Maldonado A Jr,Machado-Silva JR

    更新日期:2017-03-01 00:00:00

  • Caenorhabditis elegans and Panagrellus redivivus: enzyme-mediated modification of chemotaxis.

    abstract::Treatment with mannosidase or sialidase completely inhibited chemotactic responses of Caenorhabditis elegans wild type, C. elegans mutants CB1377 (daf-6)X and CB1379 (che-3)I, and Panagrellus redivivus to a source of attractants. Trypsin (EC3.4.21.4) caused a partial reduction in the level of chemoresponse. Normal che...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(84)90044-4

    authors: Jansson HB,Jeyaprakash A,Damon RA Jr,Zuckerman BM

    更新日期:1984-12-01 00:00:00

  • Intranasal immunization with recombinant Trichinella spiralis serine protease elicits protective immunity in BALB/c mice.

    abstract::The aim of this study was to observe the intestinal mucosal/systemic responses triggered by intranasal vaccination using recombinant Trichinella spiralis serine protease (rTsSP) and its capacity to elicit immune protection against larva challenge in a murine model. rTsSP coupled with cholera toxin B subunit (CTB) was ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2019.04.006

    authors: Sun GG,Lei JJ,Ren HN,Zhang Y,Guo KX,Long SR,Liu RD,Jiang P,Wang ZQ,Cui J

    更新日期:2019-06-01 00:00:00

  • Involvement of the Leishmania donovani virulence factor A2 in protection against heat and oxidative stress.

    abstract::Leishmania is an obligate intracellular protozoan parasite that infects cells of the reticulo-endothelial system. Host defences against Leishmania include fever and oxidant production, and the parasite has developed a number of defence mechanisms to neutralize the host response. The Leishmania donovani A2 family of pr...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2012.06.001

    authors: McCall LI,Matlashewski G

    更新日期:2012-10-01 00:00:00

  • Characterisation of iron regulatory protein 1A and 1B in the blood-feeding copepod Lepeophtheirus salmonis.

    abstract::During its parasitic life stages, the marine ectoparasitic copepod Lepeophtheirus salmonis ingests large amounts of host blood, which contains high amounts of iron. Iron is an essential micronutrient, but also toxic in high dosages, and blood-feeding parasites like the salmon louse must thus possess an efficient syste...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2015.06.010

    authors: Tröße C,Kongshaug H,Dondrup M,Nilsen F

    更新日期:2015-10-01 00:00:00

  • Ascaris suum: role of ecdysteroids in molting.

    abstract::Three studies were conducted to examine the function of ecdysteroids in the development of parasitic nematodes. Ecdysone and 20-hydroxyecdysone were extracted, separated chromatographically, and measured in the reproductive tracts of adult female Ascaris suum. Perienteric fluid and the body wall did not contain measur...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(85)90024-4

    authors: Fleming MW

    更新日期:1985-10-01 00:00:00

  • Molecular analysis of zinc transporters in Schistosoma japonicum.

    abstract::Members of the zinc-regulated transporter/iron-regulated transporter-like protein (ZIP) family of proteins transport metal ions across cell membranes. Genes encoding ZIPs are present in the genomes of schistosomes. Here, we describe molecular characterisation of six ZIPs (SjZIPA-F) from Schistosoma japonicum. Quantita...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2011.01.002

    authors: Schulte L,Glanfield A,Nawaratna S,Gobert GN,McManus DP,Jones MK

    更新日期:2011-04-01 00:00:00

  • Cellular and transcriptional changes during excystation of Giardia lamblia in vitro.

    abstract::Excystation of Giardia lamblia entails differentiation of dormant cysts into parasitic trophozoites. Despite its importance for infection, this transformation is not understood at the cellular or molecular levels. In these studies, we report that excystation entails detection of environmental stimuli across the tough ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1998.4246

    authors: Hetsko ML,McCaffery JM,Svärd SG,Meng TC,Que X,Gillin FD

    更新日期:1998-03-01 00:00:00