Abstract:
:The possible involvement of protein kinase C and Ca2+ metabolism in the proteolytic enzyme release from schistosome cercariae was studied. Cercariae were placed in dechlorinated tap water containing 0.37 mM calcium in the small glass petri dish and exposed to the stimuli (linoleic acid, phorbol esters, and Ca2+ ionophore) with or without inhibitors of protein kinase C or Ca2+ metabolism. The proteolytic activity of incubation medium of cercariae thus treated was measured by the azocoll assay. The penetration response of cercariae induced by linoleic acid, a physiological stimulus, was mimicked by phorbol esters. When exposed to phorbol esters, 0.02 to 2 microM of 12-O-tetradecanoylphorbol-13-acetate (TPA) and 0.2 to 2 microM of phorbol-12,13-dibutyrate (PDBu), cercariae ceased the swimming movement, began a rhythmic thrusting of the anterior tip of the parasite, and released the proteolytic enzyme, but they did not shed the tails. Lowering Ca2+ in water by addition of 5 mM ethylene glycol-bis(beta-aminoethyl ether) N,N,N',N'-tetraacetic acid (EGTA), phorbol ester-induced release of enzyme was completely inhibited. Phorbol ester-induced release of enzyme was partially inhibited by 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7), an inhibitor of protein kinase C, at a concentration of 100 microM. H-7 alone, at a concentration of 100 microM, did not affect the swimming movement of cercariae. The cercariae were stimulated to release the enzyme by high concentrations (10 and 100 microM) of the Ca2+ ionophore, A23187, but enzyme was not released by low concentrations (0.5 and 1 microM) of this drug. Cercariae exposed to A23187 behaved differently from those exposed to phorbol esters. They ceased swimming, showed strong muscle contraction, and shed their tail. A23187 stimulated cercariae to release the enzyme in the water containing 5 mM EGTA. A23187-induced enzyme release was not inhibited by N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), a calmodulin antagonist, trifluoperazine (TFP), a better calmodulin antagonist on schistosome, or by verapamil, a Ca2+ channel blocker. Linoleic acid-induced release of enzyme was partially inhibited by 0.5 and 5 mM of EGTA and by 1 to 100 microM of H-7. While it was not inhibited by N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide (H-8) and N-(2-guanidinoethyl)-5-isoquinolinesulfonamide (HA-1004), inhibitors of cyclic nucleotide-dependent protein kinase which were used as negative controls of H-7, W-7, TFP, 8-(N,N-diethylamino)octyl 3,4,5-trimethoxybenzoate (TMB-8), an intracellular Ca2+ antagonist, and verapamil.(ABSTRACT TRUNCATED AT 400 WORDS)
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Matsumura K,Mitsui Y,Sato K,Sakamoto M,Aoki Ydoi
10.1016/0014-4894(91)90151-lsubject
Has Abstractpub_date
1991-04-01 00:00:00pages
311-20issue
3eissn
0014-4894issn
1090-2449pii
0014-4894(91)90151-Ljournal_volume
72pub_type
杂志文章abstract::Trophozoites and cysts of free-living Acanthamoeba castellanii present a serious risk to human health as causative agents of human diseases such as fatal granulomatous amoebic encephalitis and Acanthamoeba keratitis that is reported from various part of the world, also in Poland, with increasing frequency, particularl...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2010.01.025
更新日期:2010-09-01 00:00:00
abstract::In rats, CL-Brener clone caused high mortality, severe acute myocarditis, and myositis that subsided completely in surviving animals. Accordingly, no parasite kDNA could be amplified in several organs after 4 months. The monoclonal JG strain caused null mortality, acute predominantly focal myocarditis, discrete and fo...
journal_title:Experimental parasitology
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doi:10.1016/s0014-4894(03)00119-x
更新日期:2003-05-01 00:00:00
abstract::The oocysts of Eimeria tenella, one of the most pathogenic of several species causing chicken coccidiosis, are difficult to distinguish microscopically from several other infective Eimeria species. One copy of a gene coding for 5S ribosomal RNA has been cloned from E. tenella and sequenced. A coding region of 120 nucl...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1993.1008
更新日期:1993-02-01 00:00:00
abstract::The petroleum ether extract of neem oil and its four fractions separated by column chromatography was diluted at different concentrations with liquid paraffin. The acaricidal bioassay was conducted using a dipping method. The results indicated that the median lethal concentration (LC50) of the petroleum ether extract ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2012.02.007
更新日期:2012-04-01 00:00:00
abstract::In order to examine the role of IFN-gamma, TNFRp55 and iNOS in inflammatory reaction during toxoplasmosis, IFN-gamma(-/-), TNFRp55(-/-) and iNOS(-/-) mice were experimentally infected with Toxoplasma gondii ME-49 strain. The organs of the mice were evaluated for histology and immunohistochemistry in detection of tissu...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2009.05.011
更新日期:2009-09-01 00:00:00
abstract::The sheep tapeworm (Moniezia expansa) and its host Ovis aries were analyzed by inductively coupled plasma optical emission spectrometry (ICP-OES) for their copper, iron, manganese, zinc and lead levels. Element concentrations in cestode parasites were compared to those in various organs (liver, kidney, and muscle) of ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2012.02.027
更新日期:2012-05-01 00:00:00
abstract::The kinetics of specific immunoglobulin M, A and IgG subclasses against Echinostoma caproni (Trematoda: Echinostomatidae) were analyzed in serum and intestinal fluid of two host species (Wistar rats and ICR mice) in which the course of the infection markedly differs. In rats, the worms were rapidly expelled, whereas E...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2007.02.008
更新日期:2007-08-01 00:00:00
abstract::Members of the zinc-regulated transporter/iron-regulated transporter-like protein (ZIP) family of proteins transport metal ions across cell membranes. Genes encoding ZIPs are present in the genomes of schistosomes. Here, we describe molecular characterisation of six ZIPs (SjZIPA-F) from Schistosoma japonicum. Quantita...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2011.01.002
更新日期:2011-04-01 00:00:00
abstract::Living Leishmania amazonensis amastigotes were incubated with radioiodinated N-benzyloxycarbonyl-L-tyrosyl-L-alanyl diazomethane (Z-Tyr-AlaCHN2), an irreversible inhibitor of mammalian cathepsins B and L. Parasite lysates were subjected to electrophoresis in gelatin-containing sodium dodecyl sulfate-acrylamide gels to...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(91)90066-6
更新日期:1991-11-01 00:00:00
abstract::Cutaneous Leishmaniasis (CL) caused by Leishmania aethiopica is a public health and social problem with a sequel of severe and mutilating skin lesions. It is manifested in three forms: localized CL (LCL), mucosal CL (MCL) and diffuse CL (DCL). Unresponsiveness to sodium stibogluconate (Sb(V)) is common in Ethiopian CL...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2013.01.022
更新日期:2013-05-01 00:00:00
abstract::Two different diets for the host and three drug dosage regimens were used to select lines resistant to sulfadoxine and pyrimethamine from the parent strain of the rodent malaria parasite Plasmodium berghei [the N (K173) strain]. A higher yield of resistance was obtained when a high parasitemia was present at the begin...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(83)90034-6
更新日期:1983-06-01 00:00:00
abstract::During its life cycle the malarial parasite Plasmodium forms three invasive stages which have to invade different and specific cells for replication to ensue. Invasion is vital to parasite survival and consequently proteins responsible for invasion are considered to be candidate vaccine/drug targets. Plasmodium perfor...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2007.01.015
更新日期:2007-08-01 00:00:00
abstract::Monoclonal antibodies against infective third-stage larvae (L3) of Brugia pahangi were generated from mice immunized with L3 antigens. The monoclonal antibodies were L3 stage-specific or stage-nonspecific. A BpG1 monoclonal antibody (IgG1 subclass) showing L3 stage-specificity was examined in detail. BpG1 recognized t...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(92)90130-3
更新日期:1992-08-01 00:00:00
abstract::Extracting genomic DNA of pathogenic agents from formalin-fixed specimens is inherently difficult. Storage of samples in formalin results in nucleic acid cross-linking and DNA fragmentation. In this study, DNA was extracted from 45 Giardia-positive stool samples stored in formalin and subjected to PCR amplification ta...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2019.01.010
更新日期:2019-03-01 00:00:00
abstract::Culture forms of Leishmania (Leishmania) amazonensis (IFLA/BR/67/PH8) produce an extracellular enzyme that hydrolyzes sucrose molecules into their component monosaccharides. This is important because phlebotomine sand flies, the invertebrate hosts of Leishmania, ingest plant sap or aphid and coccid honeydew rich in su...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1996.0055
更新日期:1996-06-01 00:00:00
abstract::Recombinant allergens are currently the best option for serodiagnosis of human anisakiasis in terms of sensitivity and specificity. However, previous reports showed high rates of anisakiasis patients who were negative to Ani s 7 and especially to Ani s 1. Recently, Anisakis haemoglobin was described as a major allerge...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2017.08.010
更新日期:2017-10-01 00:00:00
abstract::Increasing evidence indicates miRNAs as critical regulators of gene expression, but little information is available for miRNAs in intestinal diseases. Here, we investigated intestinal infections of male Balb/c mice with the coccidian parasite Eimeria papillata. On day 4 after oral infection, mice were shedding 3150 ± ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2010.11.002
更新日期:2011-02-01 00:00:00
abstract::Microtus fortis is a non-permissive host for Schistosoma japonicum. While M. fortis lymphocytes are known to provide natural resistance against S. japonicum, the specific mechanism remains unclear. A bone marrow transplantation (BMT) model was established using immunodeficient mice, either nude (experiment 1) or V(D)J...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2014.04.005
更新日期:2014-07-01 00:00:00
abstract::High-throughput drug screening methods against the intracellular stage of Leishmania have been facilitated by the development of in vitro models of infection. The use of cell lines rather than primary cells facilitates these methods. Peripheral blood mononuclear cell (PBMC) derived macrophages and THP-1 cells were inf...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2010.06.007
更新日期:2010-12-01 00:00:00
abstract::In 1912 Blastocystis hominis was identified as a new species and classified as a yeast (Brumpt 1912). In the early 1920s several groups confirmed its classification as a yeast, specifically a member of the genus Schizosaccharomyces (discussed by Zierdt et al. 1967). Apart from an occasional case report, the classifica...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(89)90110-0
更新日期:1989-04-01 00:00:00
abstract::BmSI-7 and BmSI-6, two Boophilus microplus subtilisin inhibitors (BmSI) were purified and characterized from eggs. The inhibitors isolated by classical purification methods presented molecular masses of 7408 and 7271Da, respectively, by MALDI-TOF-MS. Both BmSI-7 and BmSI-6 inhibited neutrophil elastase (K(i) 0.4 and 0...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2007.08.003
更新日期:2008-02-01 00:00:00
abstract::Fasciolosis in ruminants is a relentless constraint in the livestock industry across the world. Immuno-prophylactic vaccines against fasciolosis may not come up in near future, rendering the control of this scourge with chemotherapy and snail population control. With the alarming threats of anti-fasciolid drug resista...
journal_title:Experimental parasitology
pub_type: 杂志文章,评审
doi:10.1016/j.exppara.2021.108066
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abstract::Entomopathogenic nematode Heterorhabditis bacteriophora Az29 and Az36 isolates with different virulence against Popillia unipuncta and soil survival time were isolated from the Azorean archipelago (Portugal) and used for the study. RAPD analysis revealed a very low-level of genetic diversity (GD(axenic Az36 isolate)(a...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2011.12.001
更新日期:2012-02-01 00:00:00
abstract::Plasmodium vivax is the predominant species of the human malaria parasite present in the Indian subcontinent. There have been recent reports on Chloroquine (CQ) resistance and severe manifestations shown by P. vivax from different regions of the world including India. This study focuses on Bikaner, India where during ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2012.09.018
更新日期:2012-12-01 00:00:00
abstract::Monoclonal antibodies have a wide range of applications in basic and applied research as well as in the medical and pharmaceutical industries. Phage display antibody libraries offer an alternative to hybridoma technology for the generation of monoclonal antibodies and can be applied to high-throughput screening and fa...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2019.107737
更新日期:2019-10-01 00:00:00
abstract::The aim of this study was to evaluate the effects of selenium and copper on oxidative stress and its performance in lambs experimentally infected with Haemonchus contortus. Twenty-eight five-months old lambs were experimentally infected by the oral route with 5000 third-stage infective larvae and allocated into four g...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2014.06.005
更新日期:2014-09-01 00:00:00
abstract::A Taenia hydatigena model was used to assess the effect 0, 7, 14, 21, and 28 days of ensilation of minced potato on viability of tapeworm eggs. For infection of lambs, 2,000 T. hydatigena eggs were ensiled for 0, 7, 14, 21 and 28 days in minced potato at 22°C and fed to recently weaned lambs (29.9±0.76 kg). At slaught...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2013.01.003
更新日期:2013-04-01 00:00:00
abstract::Leishmaniasis are globally widespread parasitic diseases which often leads to death if left untreated. Currently available drugs present different drawbacks, so there is an urgent need to develop new, safe and cost-effective drugs against leishmaniasis. In this study we tested a small library of trans-stilbene and ter...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2016.03.007
更新日期:2016-07-01 00:00:00
abstract::Shigellosis is a serious public health problem in Korea, because large outbreaks of Shigella sonnei infections were recorded in many parts of the country during the period 1998-2000. However, the epidemiological features of shigellosis are not well known. In this study, we devised conditions suitable for the growth an...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2006.08.002
更新日期:2007-02-01 00:00:00
abstract::The tegumental alterations in adult Fasciola hepatica induced by the experimental fasciolide OZ78 were investigated utilizing scanning electron microscopy (SEM). Twelve weeks post-infection with F. hepatica, rats were treated with a single 100mg/kg oral dose of OZ78 and flukes were recovered from the bile ducts after ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2007.12.015
更新日期:2008-05-01 00:00:00