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Intranasal immunization with recombinant Trichinella spiralis serine protease elicits protective immunity in BALB/c mice.

Abstract：

:The aim of this study was to observe the intestinal mucosal/systemic responses triggered by intranasal vaccination using recombinant Trichinella spiralis serine protease (rTsSP) and its capacity to elicit immune protection against larva challenge in a murine model. rTsSP coupled with cholera toxin B subunit (CTB) was used to vaccinate mice via intranasal route. The results revealed that intranasal vaccination with rTsSP plus CTB elicited significantly intestinal local sIgA response and a TsSP-specific systemic antibody response in vaccinated mice. Furthermore, more goblet cells/acidic mucins and IgA-secreting cells were observed in jejunum from vaccinated mice. Anti-rTsSP immune serum strongly recognized the cuticle of various worm stages (muscle larva, intestinal infective larva and adult worm). The level of IFN-γ, IL-4 and IL-10 of rTsSP-vaccinated mice was significantly elevated relative to CTB and PBS control groups. The vaccinated mice exhibited a 71.10% adult reduction at 9 days pi and a 62.10% muscle larva reduction at 42 days pi following larva challenge. Additionally, vaccination with rTsSP also dampened intestinal T. spiralis development and decreased the female fecundity. Our results showed that intranasal vaccination using rTsSP adjuvanted with CTB triggered significantly local sIgA response and systemic concurrent Th1/Th2 response that induced an obvious protection against Trichinella infection.

journal_name

Exp Parasitol

journal_title

Experimental parasitology

authors

Sun GG,Lei JJ,Ren HN,Zhang Y,Guo KX,Long SR,Liu RD,Jiang P,Wang ZQ,Cui J

doi

10.1016/j.exppara.2019.04.006

subject

Has Abstract

pub_date

2019-06-01 00:00:00

pages

1-10

eissn

0014-4894

issn

1090-2449

pii

S0014-4894(19)30013-X

journal_volume

201

pub_type

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