Abstract：
:Acanthamoeba spp., known to cause keratitis and granulomatous encephalitis in humans, are frequently isolated from a variety of water sources. Here we report for the first time the characterization of an Acanthamoeba sp. (ACC01) isolated from tap water in Brazil. This organism is currently being maintained in an axenic growth medium. Phylogenetic analysis based on SSU rRNA gene sequences positioned the new isolate in genotype T4, closest to the keratitis-causing isolate, A. polyphaga ATCC 30461 ( approximately 99% similarity). Acanthamoeba ACC01 and A. polyphaga 30461 both grew at 37 degrees C and were osmotically resistant, multiplying in hyperosmolar medium. Both isolates secreted comparable amounts of proteolytic enzymes, including serine peptidases that were optimally active at a near neutral/alkaline pH and resolved identically in gelatin gels. Incubation of gels at pH 4.0 with 2mM DTT also indicated the secretion of similar cysteine peptidases. Altogether, the results point to the pathogenic potential of Acanthamoeba ACC01.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Magliano AC,da Silva FM,Teixeira MM,Alfieri SCdoi
10.1016/j.exppara.2009.07.006subject
Has Abstractpub_date
2009-11-01 00:00:00pages
231-5issue
3eissn
0014-4894issn
1090-2449pii
S0014-4894(09)00191-Xjournal_volume
123pub_type
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
pub_type： 杂志文章
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journal_title：Experimental parasitology
pub_type： 杂志文章
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journal_title：Experimental parasitology
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更新日期：2014-11-01 00:00:00
