Plasmodium falciparum: synchronization of asexual development with aphidicolin, a DNA synthesis inhibitor.

Abstract:

:The asexual development cycle of Plasmodium falciparum, a malarial parasite of humans, has been synchronized in culture by treating ring-stage parasites with aphidicolin, an inhibitor of DNA synthesis. Optimization of both the concentration of drug added to ring stage containing red blood cells and the duration of exposure of parasites to drug led to a reversible block of their maturation at the early trophozoite stage. Release of the aphidicolin block led to a synchronous development of parasites that was manifested by about 80% of the new ring stages being produced within a 2- to 3-hr interval.

journal_name

Exp Parasitol

authors

Inselburg J,Banyal HS

doi

10.1016/0014-4894(84)90061-4

subject

Has Abstract

pub_date

1984-02-01 00:00:00

pages

48-54

issue

1

eissn

0014-4894

issn

1090-2449

pii

0014-4894(84)90061-4

journal_volume

57

pub_type

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