Abstract:
:Members of the zinc-regulated transporter/iron-regulated transporter-like protein (ZIP) family of proteins transport metal ions across cell membranes. Genes encoding ZIPs are present in the genomes of schistosomes. Here, we describe molecular characterisation of six ZIPs (SjZIPA-F) from Schistosoma japonicum. Quantitative PCR analyses of these ZIPs through the lifecycle showed that each is expressed predominantly during the intramammalian stage and are particularly enriched in adult females. Using laser microdissected tissue as template, SjZIPA-D were transcriptionally enriched in female reproductive tissues, SjZIPE was not expressed in specific tissues and SjZIPF was expressed similarly in each tissue. Whole mount in situ hybridization revealed that SjZIPA and SjZIPB were localised to the oesophageal gland of adults and the vitellaria. We have shown that multiple ZIPs are expressed by schistosomes during the intramammalian parasitic phases and propose that the encoded products perform diverse cellular functions related to metal transport in different cells of S. japonicum.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Schulte L,Glanfield A,Nawaratna S,Gobert GN,McManus DP,Jones MKdoi
10.1016/j.exppara.2011.01.002subject
Has Abstractpub_date
2011-04-01 00:00:00pages
768-76issue
4eissn
0014-4894issn
1090-2449pii
S0014-4894(11)00008-7journal_volume
127pub_type
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