Abstract:
:The present work evaluates the anti-Giardia activity of Syzygium aromaticum and its major compound eugenol. The effects were evaluated on parasite growth, adherence, viability and ultrastructure. S. aromaticum essential oil (IC(50)=134 μg/ml) and eugenol (IC(50)=101 μg/ml) inhibited the growth of G. lamblia. The essential oil inhibited trophozoites adherence since the first hour of incubation and was able to kill almost 50% of the parasites population in a time dependent manner. The eugenol inhibited G. lamblia trophozoites adherence since the third hour and not induce cell lyses. The main morphological alterations were modifications on the cell shape, presence of precipitates in the cytoplasm, autophagic vesicles, internalization of flagella and ventral disc, membrane blebs, and intracellular and nuclear clearing. Taken together, our findings lead us to propose that eugenol was responsible for the anti-giardial activity of the S. aromaticum essential oil and both have potential for use as therapeutic agents against giardiasis.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Machado M,Dinis AM,Salgueiro L,Custódio JB,Cavaleiro C,Sousa MCdoi
10.1016/j.exppara.2011.01.011subject
Has Abstractpub_date
2011-04-01 00:00:00pages
732-9issue
4eissn
0014-4894issn
1090-2449pii
S0014-4894(11)00022-1journal_volume
127pub_type
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