A benzimidazole derivative (RCB20) in vitro induces an activation of energetic pathways on Taenia crassiceps (ORF strain) cysticerci.

abstract：:Members of the zinc-regulated transporter/iron-regulated transporter-like protein (ZIP) family of proteins transport metal ions across cell membranes. Genes encoding ZIPs are present in the genomes of schistosomes. Here, we describe molecular characterisation of six ZIPs (SjZIPA-F) from Schistosoma japonicum. Quantita...

journal_title：Experimental parasitology

authors： Schulte L,Glanfield A,Nawaratna S,Gobert GN,McManus DP,Jones MK

更新日期：2011-04-01 00:00:00

abstract：:Activation of the hypothalamus-pituitary-adrenal axis plays a major role in the suppression of the immune system. We have investigated the effects of repetitive stress on Wistar rats infected with the Y strain of Trypanosoma cruzi and a control group that underwent stressor stimuli by exposure to ether vapor for one m...

journal_title：Experimental parasitology

authors： Santos CD,Caldeira JC,Toldo MP,Prado JC

更新日期：2005-06-01 00:00:00

abstract：:Giardia lamblia consist of heterogeneous isolates that can be divided into at least three groups. Differential screening of a cDNA library with isolate-specific antisera identified a gene which is expressed and found only in Group 3 isolates. This gene, GLORF-C4, is 597 bp in length and predicts a deduced protein of 1...

journal_title：Experimental parasitology

authors： Nash TE,Mowatt MR

更新日期：1992-12-01 00:00:00

abstract：:High-throughput drug screening methods against the intracellular stage of Leishmania have been facilitated by the development of in vitro models of infection. The use of cell lines rather than primary cells facilitates these methods. Peripheral blood mononuclear cell (PBMC) derived macrophages and THP-1 cells were inf...

journal_title：Experimental parasitology

authors： Mehta SR,Zhang XQ,Badaro R,Spina C,Day J,Chang KP,Schooley RT

更新日期：2010-12-01 00:00:00

abstract：:By using a sensitive fluorometric method with Val-Leu-Gly-Arg-3-amino-9-ethylcarbazole (VLGR-AEC) as a substrate, two endopeptidase activities were identified in two fractions of Sephacryl S-200 gel filtration from soluble P. berghei and P. chabaudi extracts. Controls with normal mouse erythrocytes, with leukocytes, a...

journal_title：Experimental parasitology

authors： Bernard F,Mayer R,Picard I,Deguercy A,Monsigny M,Schrevel J

更新日期：1987-08-01 00:00:00

abstract：:Cystic echinococcosis (CE) is caused by the larval stage of Echinococcus granulosus, which in this disease the metacestode develop in visceral organs especially liver and lungs. The disease is present worldwide and affects humans as well as herbivores including cattle, sheep, camels, horses and others. Benzimidazole c...

journal_title：Experimental parasitology

authors： Ahmadnia S,Moazeni M,Mohammadi-Samani S,Oryan A

更新日期：2013-10-01 00:00:00

abstract：:An NADP-linked glutamate dehydrogenase (EC 1.4.1.4) was found in the soluble fraction of Trichomonas vaginalis. Its molecular weight was about 230,000 (gel filtration). The enzyme, partially purified by diafiltration and hydroxyapatite column chromatography, was heat stable (1 hr at 57 C). It catalyzed both the aminat...

journal_title：Experimental parasitology

authors： Turner AC,Lushbaugh WB

更新日期：1988-10-01 00:00:00

abstract：:Three-day-old specific-pathogen-free chickens were subcutaneously immunized with Eimeria tenella recombinant 5401 antigen (100 microg per chicken) with (0.25, 0.5 or 1.0mg per dose) or without ginsenosides, and boosted with the same dosage 14 days later. The chickens were challenged with 6 x 10(4) homologous sporulate...

journal_title：Experimental parasitology

authors： Du A,Hu S,Wang S

更新日期：2005-11-01 00:00:00

abstract：:Infection with Leishmania infantum causes the disease visceral leishmaniasis (VL), which is a serious clinical and veterinary problem. The drugs used to treat canine leishmaniasis (CanL) do not cause complete parasite clearance; they can be toxic, and emerging drug resistance in parasite populations limits their clini...

journal_title：Experimental parasitology

authors： Miret JA,Moreno J,Nieto J,Carter KC,Mullen AB,Ambros L,Rodríguez C,San Andrés MI,González F

更新日期：2021-01-01 00:00:00

abstract：:Parasites of the Leishmania genus require for the growth and viability the de novo synthesis of specific sterols as such as episterol and 5-dehydroepisterol because cholesterol, which is abundant in their mammalian hosts, does not fulfill the parasite sterol requirements. Squalene synthase catalyzes the first committe...

journal_title：Experimental parasitology

authors： Rodrigues JC,Urbina JA,de Souza W

更新日期：2005-12-01 00:00:00

abstract：:Polymerase chain reaction amplification of several polymorphic genes has been used to study the population biology of Plasmodium falciparum. S antigen is particularly suitable for such studies, but difficulties in the amplification of this gene have precluded its use. Here we describe a simple method for the amplifica...

journal_title：Experimental parasitology

authors： Kyes S,Craig AG,Marsh K,Newbold CI

更新日期：1993-12-01 00:00:00

abstract：:A full-length cDNA encoding the 24-kDa flagellar Ca(2+)-binding protein (FCaBP) of the Dm28c clone of Trypanosoma cruzi was cloned and characterized. Comparison of the deduced amino acid sequence with those of the FCaBPs of other T. cruzi strains revealed greater than 97% sequence conservation. FCaBP-like genes are fo...

journal_title：Experimental parasitology

authors： Maldonado RA,Linss J,Thomaz N,Olson CL,Engman DM,Goldenberg S

更新日期：1997-07-01 00:00:00

abstract：:Toxoplasmosis is a widely distributed disease caused by the protozoan Toxoplasma gondii that is mainly transmitted orally. Once ingested, the parasite crosses the intestinal barrier to reach the blood and lymph systems to migrate to other regions of the host. The objective of this study was to evaluate the changes in ...

journal_title：Experimental parasitology

authors： Vicentino-Vieira SL,Nogueira de Melo Gde A,Biondaro Góis M,Martins Moreira N,de Araujo Pereira LG,de Almeida Araújo EJ,Garcia JL,de Mello Gonçales Sant'Ana D

更新日期：2015-09-01 00:00:00

abstract：:The cDNA clone coding a major vault protein (MVP)-like protein was derived from Echinococcus multilocularis cysts. MVP is a main component of vault particles, which are the largest cytoplasmic ribonucleoprotein particles in eukaryotic cells. We sequenced and characterized E. multilocularis MVP (EmMVP). The nucleotide ...

journal_title：Experimental parasitology

authors： Goto A,Kouguchi H,Yamano K,Sawada Y

更新日期：2013-05-01 00:00:00

abstract：:African trypanosomes exhibit dramatic developmental changes in carbohydrate and energy metabolism, with cytochrome-mediated respiration playing an important role only in the insect stages. The parasites possess three isozymes of malate dehydrogenase, each of which has a different subcellular localization and, likely, ...

journal_title：Experimental parasitology

authors： Anderson SA,Carter V,Parsons M

更新日期：1998-05-01 00:00:00

abstract：:To understand the genetic basis of virulence, gene expression profiles of a temperature-sensitive clone (NCts-8, relatively avirulent) and its wild type (NC-1) of Neospora caninum were characterized and compared using a high-density microarray with approximately 63,000 distinct oligonucleotides. This microarray consis...

journal_title：Experimental parasitology

authors： Li RW,Tuo W

更新日期：2011-12-01 00:00:00

abstract：:The contribution of mononuclear phagocytes to host resistance against acute Chagas disease has been studied in vivo in a rat model inoculated with Trypanosoma cruzi, Y strain. Acute T. cruzi infection triggered a dramatic increase (937%) in peripheral blood monocyte number at day 12 of infection. At this point, histol...

journal_title：Experimental parasitology

authors： Melo RC,Machado CR

更新日期：2001-01-01 00:00:00

abstract：:With the aim of determining selectivity and the possible target(s) of nitroheteroaryl-1,3,4-thiadiazoles considered as possible leads for the development of anti-leishmanial agents, we studied 5-nitroimidazole, 5-nitrofuran and 5-nitrothiophene analogs of N-substituted-piperazinyl-1,3,4-thiadiazoles. We investigated 2...

journal_title：Experimental parasitology

authors： Poorrajab F,Ardestani SK,Foroumadi A,Emami S,Kariminia A,Behrouzi-Fardmoghadam M,Shafiee A

更新日期：2009-04-01 00:00:00

abstract：:Six chimpanzees (Pan troglodytes) and six mangabey monkeys (Cercocebus atys) were inoculated with Onchocerca volvulus third-stage larvae (L3) of West African origin. Two chimpanzees each received 200, 300, or 400 L3, while three mangabeys each received either 50 or 250 L3. All six chimpanzees became microfilaria posit...

journal_title：Experimental parasitology

authors： Eberhard ML,Dickerson JW,Tsang VC,Walker EM,Ottesen EA,Chandrashekar R,Weil GJ,Trpis M,Strobert E,Constantinidis I

更新日期：1995-05-01 00:00:00

abstract：:In murine Schistosoma mansoni infections, fewer adult worms develop in male than in female mice infected with the same number of cercariae. To evaluate a potential role for testosterone in this phenomenon, testosterone levels were manipulated in groups of CBA/J mice that were then infected and monitored for survival r...

journal_title：Experimental parasitology

authors： Nakazawa M,Fantappie MR,Freeman GL Jr,Eloi-Santos S,Olsen NJ,Kovacs WJ,Secor WE,Colley DG

更新日期：1997-03-01 00:00:00

abstract：:The glycosomes of trypanosomatids are essential organelles that are evolutionarily related to peroxisomes of other eukaryotes. The peroxisomal RING proteins-PEX2, PEX10 and PEX12-comprise a network of integral membrane proteins that function in the matrix protein import cycle. Here, we describe PEX10 and PEX12 in Tryp...

journal_title：Experimental parasitology

authors： Saveria T,Kessler P,Jensen BC,Parsons M

更新日期：2007-05-01 00:00:00

abstract：:Antimony unresponsiveness in mucocutaneous and visceral leishmaniasis is a serious clinical problem. Information on the mechanisms and characteristics of drug resistance in parasites that suggest chemotherapeutic strategies to overcome resistance is of practical importance. We developed nine lines of Leishmania resist...

journal_title：Experimental parasitology

authors： Grögl M,Oduola AM,Cordero LD,Kyle DE

更新日期：1989-07-01 00:00:00

abstract：:Lipophosphoglycan (LPG) is structurally characterized by a series of phosphoglycan repeat units. Cellular LPG, isolated from promastigotes, has a very similar structure to culture supernatant LPG, but differs in the average number of phosphorylated oligosaccharide repeat units and in glycan composition. Comparison of ...

journal_title：Experimental parasitology

authors： Kavoosi G,Ardestani SK,Kariminia A,Alimohammadian MH

更新日期：2010-02-01 00:00:00

abstract：:Large numbers (10(4) to greater than 10(5)/ml) of Type I water-resistant Giardia lamblia cysts were produced in vitro under conditions that are characteristic of the human intestinal lumen. We define Type I cyst morphology as oval shaped, smooth, and refractile, with cyst wall, axostyle, and median body visible in rel...

journal_title：Experimental parasitology

authors： Gillin FD,Boucher SE,Rossi SS,Reiner DS

更新日期：1989-08-01 00:00:00

abstract：:The diagnostic potential of recombinant E/S antigens of the lymphatic filaria Brugia malayi was investigated by Western blot. A cDNA expression library was constructed using B. malayi male adult worm mRNA, and E/S recombinants were identified with a rabbit antiserum raised against E/S products collected in vitro from ...

journal_title：Experimental parasitology

authors： Kumari S,Lillibridge CD,Bakeer M,Lowrie RC Jr,Jayaraman K,Philipp MT

更新日期：1994-12-01 00:00:00

abstract：:Infective larvae of Strongyloides spp. have been shown to contain azocollytic enzymes which may aid in host skin penetration. Attempts to demonstrate classical, neutral pH-active collagenase activity in Strongyloides ratti were unsuccessful. In the current study, we investigated the presence of acidic collagenolytic a...

journal_title：Experimental parasitology

authors： Rege AA,Dresden MH

更新日期：1987-12-01 00:00:00

abstract：:Schistosomiasis remains a global health problem. In the Mekong river basin, approximately 80,000 people are at risk of infection by Schistosoma mekongi. The parasite's eggs become entrapped in the host's organs and induce massive inflammation, contributing to the pathogenesis of schistosomiasis. In addition, egg antig...

journal_title：Experimental parasitology

authors： Thiangtrongjit T,Adisakwattana P,Limpanont Y,Dekumyoy P,Nuamtanong S,Chusongsang P,Chusongsang Y,Reamtong O

更新日期：2018-08-01 00:00:00

abstract：:Serine proteases have been identified as important molecules that are involved in many parasitic infections, and these molecules have also been suggested to play important roles in Trichinella spiralis infections. In the present study, the antigenic serine protease gene Ts-ADSp-7, which was screened from a cDNA librar...

journal_title：Experimental parasitology

authors： Gao H,Tang B,Bai X,Wang L,Wu X,Shi H,Wang X,Liu X,Liu M

更新日期：2018-12-01 00:00:00

abstract：:Chagas disease is a tropical and systemic disease caused by the parasite Trypanosoma cruzi. This parasite has been divided into six Discrete Typing Units (DTU's) due to its high genetic diversity. T. cruzi I (TcI) is the most prevalent DTU in Colombia and recently associated to cardiomyopathies. The aim of this study ...

journal_title：Experimental parasitology

authors： Ramírez JD,Duque MC,Montilla M,Cucunubá Z,Guhl F

更新日期：2012-12-01 00:00:00

abstract：:Automated extraction of DNA for testing of laboratory samples is an attractive alternative to labour-intensive manual methods when higher throughput is required. However, it is important to maintain the maximum detection sensitivity possible to reduce the occurrence of type II errors (false negatives; failure to detec...

journal_title：Experimental parasitology

authors： Dunlop J,Thompson CK,Godfrey SS,Thompson RC

更新日期：2014-11-01 00:00:00