Abstract:
:The glycosomes of trypanosomatids are essential organelles that are evolutionarily related to peroxisomes of other eukaryotes. The peroxisomal RING proteins-PEX2, PEX10 and PEX12-comprise a network of integral membrane proteins that function in the matrix protein import cycle. Here, we describe PEX10 and PEX12 in Trypanosoma brucei, Leishmania major, and Trypanosoma cruzi. We expressed GFP fusions of each T. brucei coding region in procyclic form T. brucei, where they localized to glycosomes and behaved as integral membrane proteins. Despite the weak transmembrane predictions for TbPEX12, protease protection assays demonstrated that both the N and C termini are cytosolic, similar to mammalian PEX12. GFP fusions of T. cruzi PEX10 and L. major PEX12 also localized to glycosomes in T. brucei indicating that glycosomal membrane protein targeting is conserved across trypanosomatids.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Saveria T,Kessler P,Jensen BC,Parsons Mdoi
10.1016/j.exppara.2006.11.004subject
Has Abstractpub_date
2007-05-01 00:00:00pages
14-24issue
1eissn
0014-4894issn
1090-2449pii
S0014-4894(06)00295-5journal_volume
116pub_type
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journal_title:Experimental parasitology
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