Plasmodium falciparum: a method for the amplification of S antigens and its application to laboratory and field samples.

Abstract:

:Polymerase chain reaction amplification of several polymorphic genes has been used to study the population biology of Plasmodium falciparum. S antigen is particularly suitable for such studies, but difficulties in the amplification of this gene have precluded its use. Here we describe a simple method for the amplification of S antigen and show why previous attempts may have been unsuccessful. Data are presented from both laboratory and field isolates.

journal_name

Exp Parasitol

authors

Kyes S,Craig AG,Marsh K,Newbold CI

doi

10.1006/expr.1993.1108

subject

Has Abstract

pub_date

1993-12-01 00:00:00

pages

473-83

issue

4

eissn

0014-4894

issn

1090-2449

pii

S0014-4894(83)71108-2

journal_volume

77

pub_type

杂志文章
  • Leishmania major lipophosphoglycan: discrepancy in Toll-like receptor signaling.

    abstract::Lipophosphoglycan (LPG) is structurally characterized by a series of phosphoglycan repeat units. Cellular LPG, isolated from promastigotes, has a very similar structure to culture supernatant LPG, but differs in the average number of phosphorylated oligosaccharide repeat units and in glycan composition. Comparison of ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2009.09.017

    authors: Kavoosi G,Ardestani SK,Kariminia A,Alimohammadian MH

    更新日期:2010-02-01 00:00:00

  • Echinococcus granulosus: induction of T-independent antibody response against protoscolex glycoconjugates in early experimental infection.

    abstract::In this work CD4-knockout mice were used as a model to analyse the role of CD4+ T cells in the antibody response against Echinococcus granulosus immunization or experimental infection. Results obtained with mice immunized with protoscolex antigens indicated that these contain T-independent antigens. After infection, C...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2008.04.024

    authors: Baz A,Carol H,Fernández V,Mourglia-Ettlin G,Nieto A,Orn A,Dematteis S

    更新日期:2008-08-01 00:00:00

  • Schistosoma mansoni: possible involvement of protein kinase C in linoleic acid-induced proteolytic enzyme release from cercariae.

    abstract::The possible involvement of protein kinase C and Ca2+ metabolism in the proteolytic enzyme release from schistosome cercariae was studied. Cercariae were placed in dechlorinated tap water containing 0.37 mM calcium in the small glass petri dish and exposed to the stimuli (linoleic acid, phorbol esters, and Ca2+ ionoph...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(91)90151-l

    authors: Matsumura K,Mitsui Y,Sato K,Sakamoto M,Aoki Y

    更新日期:1991-04-01 00:00:00

  • Generation of a single-chain variable fragment phage display antibody library from naïve mice panned against Fasciola hepatica antigens.

    abstract::Monoclonal antibodies have a wide range of applications in basic and applied research as well as in the medical and pharmaceutical industries. Phage display antibody libraries offer an alternative to hybridoma technology for the generation of monoclonal antibodies and can be applied to high-throughput screening and fa...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2019.107737

    authors: Norbury LJ,Basałaj K,Bąska P,Zawistowska-Deniziak A,Kalinowska A,Wilkowski P,Wesołowska A,Wędrychowicz H

    更新日期:2019-10-01 00:00:00

  • Antibody response in dogs experimentally infected with Leishmania infantum: infection course antigen markers.

    abstract::Five-month-old beagle dogs were experimentally infected with amastigotes of Leishmania infantum and kept for 14 months. Infection course was monitored by clinical examination, serum protein variations, and levels of specific antibodies against Leishmania estimated by indirect immunofluorescence test and Western blotti...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1996.0018

    authors: Carrera L,Fermín ML,Tesouro M,García P,Rollán E,González JL,Méndez S,Cuquerella M,Alunda JM

    更新日期:1996-03-01 00:00:00

  • Haemonchus contortus: molecular cloning, sequencing, and expression analysis of the gene coding for the small subunit of ribonucleotide reductase.

    abstract::Gastro-intestinal (GI) parasites are of great agricultural importance, annually costing the livestock industry vast amounts in resources to control parasitism. One such GI parasite, Haemonchus contortus, is principally pathogenic to sheep; with the parasite's blood-feeding behaviour causing effects ranging from mild a...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2005.08.001

    authors: Chen Y,Donald D,Savin K,Presidente PJ,Hartman D

    更新日期:2005-12-01 00:00:00

  • Identification and characterization of a Giardia lamblia group-specific gene.

    abstract::Giardia lamblia consist of heterogeneous isolates that can be divided into at least three groups. Differential screening of a cDNA library with isolate-specific antisera identified a gene which is expressed and found only in Group 3 isolates. This gene, GLORF-C4, is 597 bp in length and predicts a deduced protein of 1...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(92)90250-e

    authors: Nash TE,Mowatt MR

    更新日期:1992-12-01 00:00:00

  • Haemonchus contortus: in vitro and in vivo anthelmintic activity of aqueous and hydro-alcoholic extracts of Hedera helix.

    abstract::In vitro anthelmintic activity of crude extracts of the ripe fruits of Hedera helix was investigated on eggs and adult nematode parasites Haemonchus contortus. Aqueous extract of H. helix was also evaluated for in vivo anthelmintic activity at dose of 1.13 and 2.25 g/kg in sheep artificially infected with H. contortus...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2007.01.019

    authors: Eguale T,Tilahun G,Debella A,Feleke A,Makonnen E

    更新日期:2007-08-01 00:00:00

  • Diversity of Sarcocystis spp shed by opossums in Brazil inferred with phylogenetic analysis of DNA coding ITS1, cytochrome B, and surface antigens.

    abstract::Although few species of Sarcocystis are known to use marsupials of the genus Didelphis as definitive host, an extensive diversity of alleles of surface antigen genes (sag2, sag3, and sag4) has been described in samples of didelphid opossums in Brazil. In this work, we studied 25 samples of Sarcocystis derived from gas...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2016.02.008

    authors: Valadas SY,da Silva JI,Lopes EG,Keid LB,Zwarg T,de Oliveira AS,Sanches TC,Joppert AM,Pena HF,Oliveira TM,Ferreira HL,Soares RM

    更新日期:2016-05-01 00:00:00

  • Grillotia erinaceus (Cestoda, Trypanorhyncha): localization of neuroactive substances in the plerocercoid, using confocal and electron-microscopic immunocytochemistry.

    abstract::Indirect immunocytochemistry, in conjunction with confocal scanning laser microscopy and electron-microscopic immunogold labeling, has been used to localize neuropeptide and 5-hydroxytryptamine (5-HT) immunoreactivities (IRs) in the plerocercoid (scolex and surrounding blastocyst) of the trypanorhynch tapeworm, Grillo...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1994.1103

    authors: Halton DW,Maule AG,Brennan GP,Shaw C,Stoitsova SR,Johnston CF

    更新日期:1994-11-01 00:00:00

  • Insights into the biological features of the antigenic determinants recognized by four monoclonal antibodies in redia and adult stages of the liver fluke Fasciola hepatica.

    abstract::Fasciola hepatica is a digenean trematode which infects a wide variety of domestic animals and also humans. Previous studies have demonstrated that four monoclonal antibodies (Mabs) against the total extract of F. hepatica redia (named as 1E4, 6G11, 4E5 and 4G11) also recognized the excretion - secretion antigens (ES ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2016.06.010

    authors: Alba A,Sánchez J,Hernández H,Mosqueda M,Rodríguez SY,Capó V,Otero O,Alfonso C,Marcet R,Sarracent J

    更新日期:2016-09-01 00:00:00

  • Trypanosoma evansi: molecular homogeneity as inferred by phenetical analysis of ribosomal internal transcribed spacers DNA of an eclectic parasite.

    abstract::The protozoan Trypanosoma evansi is described as presenting high morphological and genetic similarities among the isolates despite its biological heterogeneity and wide geographical distribution. PCR amplification of the internal transcribed spacers of the ribosomal gene in combination with the coding region of the 5....

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2007.10.003

    authors: de Oliveira Lima AN,da Silva Santos S,Herrera HM,Gama C,Cupolillo E,Jansen AM,Fernandes O

    更新日期:2008-03-01 00:00:00

  • Plasmodium falciparum: variations in the C-terminal cysteine-rich region of the merozoite surface protein-1 in field samples among Indian isolates.

    abstract::The cysteine-rich C-terminal region of the merozoite surface protein-1, MSP-119, of Plasmodium falciparum has been the most promising vaccine target antigen to date, based on protective immunization studies with recombinant proteins in mice and monkey models. To be further developed as a vaccine candidate, it is essen...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1999.4401

    authors: Lalitha PV,Malhotra P,Chattopadhyay R,Chauhan VS

    更新日期:1999-05-01 00:00:00

  • Trypanosoma brucei: molecular cloning and stage-regulated expression of a malate dehydrogenase localized to the mitochondrion.

    abstract::African trypanosomes exhibit dramatic developmental changes in carbohydrate and energy metabolism, with cytochrome-mediated respiration playing an important role only in the insect stages. The parasites possess three isozymes of malate dehydrogenase, each of which has a different subcellular localization and, likely, ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1998.4260

    authors: Anderson SA,Carter V,Parsons M

    更新日期:1998-05-01 00:00:00

  • Schistosoma mansoni: a new parenchymal cell in cercariae.

    abstract::A new type of cell has been identified in cercariae of Schistosoma mansoni. The perikarya (cell bodies) of these cells were located in the body (midsegment), in an area oral to the acetabulum (ventral sucker). Cytoplasmic processes extending from the perikarya ramified throughout the parenchyma of the anterior organ (...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(86)90132-3

    authors: Dorsey CH,Cousin CE

    更新日期:1986-02-01 00:00:00

  • Prevalence and characterization of Cryptosporidium spp. in dairy cattle in Nile River delta provinces, Egypt.

    abstract::Molecular characterizations of Cryptosporidium spp. in dairy cattle in industrialized nations have mostly shown a dominance of Cryptosporidium parvum, especially its IIa subtypes in pre-weaned calves. Few studies, however, have been conducted on the distribution of Cryptosporidium species and C. parvum subtypes in var...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2013.09.002

    authors: Amer S,Zidan S,Adamu H,Ye J,Roellig D,Xiao L,Feng Y

    更新日期:2013-11-01 00:00:00

  • Small animal in vivo imaging of parasitic infections: A systematic review.

    abstract::Non-invasive small animal in vivo imaging is an essential tool in a broad variety of biomedical sciences and enables continuous monitoring of disease progression in order to develop and improve diagnostic, therapeutic and preventive measures. Imaging parasites non-invasively in live animals allows efficient parasite d...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2020.107905

    authors: Novobilský A,Höglund J

    更新日期:2020-07-01 00:00:00

  • Schistosoma mansoni: surface membrane stability in vitro and in vivo.

    abstract::The human complement component C3b is known to bind in vitro to the surfaces of all developmental stages of schistosomes as a consequence of complement activation by the alternative pathway. C3b bound to Schistosoma mansoni parasites has now been used in combination with fluorescent labeled antibodies against C3b to l...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(86)90027-5

    authors: Ruppel A,McLaren DJ

    更新日期:1986-10-01 00:00:00

  • Leishmania donovani: surface membrane acid phosphatase blocks neutrophil oxidative metabolite production.

    abstract::We show that a purified preparation of the prominent tartrate-resistant acid phosphatase (E.C.3.1.3.2), isolated from the external surface of the intracellular parasite Leishmania donovani (promastigote form), inhibits toxic oxidative metabolite production of neutrophils. Preincubation of a neutrophil suspension (2.5 ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(85)90039-6

    authors: Remaley AT,Glew RH,Kuhns DB,Basford RE,Waggoner AS,Ernst LA,Pope M

    更新日期:1985-12-01 00:00:00

  • Toxoplasma gondii: over-expression of lactate dehydrogenase enhances differentiation under alkaline conditions.

    abstract::Toxoplasma gondii, an intracellular parasite, has two distinctive growth stages, namely rapidly growing tachyzoites and slowly growing bradyzoites. Here we report a unique physiological function of the last committed glycolytic enzyme of T. gondii, lactate dehydrogenase (TgLDH), which is present in two isoforms and ex...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2009.01.016

    authors: Liwak U,Ananvoranich S

    更新日期:2009-06-01 00:00:00

  • Cryptosporidium species and Cryptosporidium parvum subtypes in dairy calves and goat kids reared under traditional farming systems in Turkey.

    abstract::Molecular characterizations of Cryptosporidium spp. in ruminants reared under traditional animal management systems are scarce and studies conducted thus far have revealed largely an absence of the pathogenic and zoonotic species Cryptosporidium parvum in pre-weaned animals. In this study, we examined Cryptosporidium ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2016.06.014

    authors: Taylan-Ozkan A,Yasa-Duru S,Usluca S,Lysen C,Ye J,Roellig DM,Feng Y,Xiao L

    更新日期:2016-11-01 00:00:00

  • Movable computer ruler (MCR): a new method for measuring the size of Toxoplasma gondii cysts, tachyzoites and other selected parasites.

    abstract::A new method for measuring the size of parasites and other objects using optical microscopy was developed using a specifically designed movable computer ruler (MCR) derived from digital images of a stage micrometer. Subsequently, MCR can be superimposed on images of parasites to measure their size. MCR derived from th...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2011.10.008

    authors: Otify YZ

    更新日期:2012-01-01 00:00:00

  • Oxidative stress associated with pathological lesions in the liver of rats experimentally infected by Fasciola hepatica.

    abstract::The aim of this study was to analyze the antioxidant status and oxidative profile in serum and liver of rats experimentally infected with Fasciola hepatica and its relationship with pathological findings. Twenty-four rats were divided into two groups: group A consisted of 12 healthy rats and group B of 12 rats infecte...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2015.08.008

    authors: Bottari NB,Mendes RE,Lucca NJ,Schwertz CI,Henker LC,Olsson DC,Piva MM,Sangoi M,Campos LP,Moresco RN,Jaques JA,Da silva AS

    更新日期:2015-12-01 00:00:00

  • Glycosidases in Leishmania and their importance for Leishmania in phlebotomine sandflies with special reference to purification and characterization of a sucrase.

    abstract::Culture forms of Leishmania (Leishmania) amazonensis (IFLA/BR/67/PH8) produce an extracellular enzyme that hydrolyzes sucrose molecules into their component monosaccharides. This is important because phlebotomine sand flies, the invertebrate hosts of Leishmania, ingest plant sap or aphid and coccid honeydew rich in su...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1996.0055

    authors: Gontijo NF,Melo MN,Riani EB,Almeida-Silva S,Mares-Guia ML

    更新日期:1996-06-01 00:00:00

  • Standardization of DNA extraction from sand flies: Application to genotyping by next generation sequencing.

    abstract::Standardization of the methods for extraction of DNA from sand flies is essential for obtaining high efficiency during subsequent molecular analyses, such as the new sequencing methods. Information obtained using these methods may contribute substantially to taxonomic, evolutionary, and eco-epidemiological studies. Th...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2017.04.010

    authors: Casaril AE,de Oliveira LP,Alonso DP,de Oliveira EF,Gomes Barrios SP,de Oliveira Moura Infran J,Fernandes WS,Oshiro ET,Ferreira AMT,Ribolla PEM,de Oliveira AG

    更新日期:2017-06-01 00:00:00

  • A family of serine protease inhibitors (serpins) in the cattle tick Rhipicephalus (Boophilus) microplus.

    abstract::Proteins belonging to the serine protease inhibitor (serpin) superfamily play essential roles in many organisms. In arthropods these proteins are involved in innate immune system, morphogenesis and development. In mammals serpins regulate pathways that are essential to life such as blood coagulation, fibrinolysis, inf...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2013.12.001

    authors: Tirloni L,Seixas A,Mulenga A,Vaz Ida S Jr,Termignoni C

    更新日期:2014-02-01 00:00:00

  • Phylogenetic position of Leishmania isolates from Khyber Pakhtunkhwa province of Pakistan.

    abstract::Several species of the genus Leishmania are causative agents of cutaneous leishmaniasis in Pakistan. This study aimed to determine phylogenetic placement of Leishmania species causing cutaneous leishmaniasis in Khyber Pakhtunkhwa province, Pakistan (34 Leishmania tropica, 3 Leishmania infantum), in-relation to species...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2016.05.006

    authors: Khan NH,Messenger LA,Wahid S,Sutherland CJ

    更新日期:2016-08-01 00:00:00

  • Preventive and therapeutic effects of auxotrophic Edwardsiella tarda mutant harboring CpG 1668 motif-enriched plasmids against scuticociliatosis in olive flounder (Paralichthys olivaceus).

    abstract::Previously generated two auxotrophic genes knockout Edwardsiella tarda (Δalr Δasd E. tarda) was used as a delivery vehicle for CpG 1668 motif-enriched plasmids (pL-CpG30), and potential of the Δalr Δasd E. tarda-mediated delivery of CpG motifs in both prevention and therapeutic treatment of scuticociliatosis caused by...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2014.06.007

    authors: Kang YJ,Choi SH,Kim KH

    更新日期:2014-09-01 00:00:00

  • Plasmodium chabaudi chabaudi: B-1 cell expansion correlates with semiresistance in BALB/cJ mice.

    abstract::The largest obstacle impeding the development of an effective malaria vaccine is the incomplete understanding of how the immune response is regulated during infection. B-1a cells, a poorly understood subcategory of B lymphocytes, produce nonpathologic autoantibodies of low affinity which have been shown to have distin...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.2001.4622

    authors: Yoder BJ,Goodrum KJ

    更新日期:2001-06-01 00:00:00

  • Brugia pahangi: production of a monoclonal antibody reactive with the surface of infective larvae.

    abstract::Monoclonal antibodies against infective third-stage larvae (L3) of Brugia pahangi were generated from mice immunized with L3 antigens. The monoclonal antibodies were L3 stage-specific or stage-nonspecific. A BpG1 monoclonal antibody (IgG1 subclass) showing L3 stage-specificity was examined in detail. BpG1 recognized t...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(92)90130-3

    authors: Oikawa Y,Ikeda T,Horii Y,Fujita K,Tsukidate S

    更新日期:1992-08-01 00:00:00